Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- AB0100-200 - Provider product page
- Provider
- SICGEN
- Proper citation
- SICGEN Cat#AB0100-200, RRID:AB_2333126
- Product name
- Anti-CD45
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody to CD45. It is a 180-220 kDa type I transmembrane glycoprotein and a member of the protein tyrosine phosphatase (PTP) family. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus is classified as a receptor type PTP. It is expressed in all hematopoietic cells, but highest on lymphocytes. PTPs are known to be signalling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitosis, and oncogenic transformation. It has intrinsic tyrosine phosphatase activity and it is essential for T and B cell antigen receptor-mediated activation. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signalling. CD45 also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signalling. The cross-linking of CD45 induces apoptosis in lymphocytes.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Donkey, Feline, Goat, Guinea Pig, Hamster, Horse, Porcine, Rabbit, Sheep, Simian, Other
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
EAEDLQVGQVELGGGPGAGSLQPLALEGSLQGT-E
VEDPQVAQLELGGGPGAGDLQTLALEVARQ- Isotype
- IgG
- Vial size
- 600 µg
- Concentration
- 3 mg/ml
- Storage
- Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
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- SICGEN (provider)
- Main image
- Experimental details
- Endogenous CD45 detected with AB0100 at 1/500 dilution; lysate at 100 µg per lane
- Sample type
- Jurkat cell line
- Primary Ab dilution
- 1/500
- Conjugate
- Unconjugated
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1/10,000