Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [2]
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Validation data
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- Product number
- NBP1-79127AF647 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal CD45 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. The mouse monoclonal antibody HI30 recognizes all isoforms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets. HLDA IV
- Reactivity
- Human, Simian
- Host
- Mouse
- Conjugate
- Red dye
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C in the dark.
Submitted references Evaluation of PD-L1/PD-1 on circulating tumor cells in patients with advanced non-small cell lung cancer.
Kallergi G, Vetsika EK, Aggouraki D, Lagoudaki E, Koutsopoulos A, Koinis F, Katsarlinos P, Trypaki M, Messaritakis I, Stournaras C, Georgoulias V, Kotsakis A
Therapeutic advances in medical oncology 2018;10:1758834017750121
Therapeutic advances in medical oncology 2018;10:1758834017750121
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Cell Surface): CD45 Antibody (HI30) [Alexa Fluor (R) 647] [NBP1-79127AF647] - Cell surface staining of PBMC's (Lymphocyte gate at 1 x 10^6 cells/ml) with CD45 antibody (orange) stained at a dilution of 1:500. Shown with mIgG1 (AF647 conjugated) Isotype control (blue).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): CD45 Antibody (HI30) [Alexa Fluor (R) 647] [NBP1-79127AF647] - Intracellular staining of hPBMC cells (1 x 10^6 cells/ml) with AlexaFluor 647 conjugated anti-CD45 antibody (blue) stained at a dilution of 1:500. Shown with the Istotype control (orange).