Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [2]
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Validation data
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- Product number
- MA5-18142 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD45 Monoclonal Antibody (MEM-28), Alexa Fluor™ 647
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- This antibody reacts with an extracellular epitope on all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets. This antibody will not cross-react with equine.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Red dye
- Isotype
- IgG
- Antibody clone number
- MEM-28
- Vial size
- 100 Tests
- Storage
- 4° C, store in dark, DO NOT FREEZE!
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD45 (MEM-28) Alexa Fluor® 647 Monoclonal antibody (Product # MA5-18142) using a dilution of 4 µL reagent/100 µL of peripheral whole blood.
- Conjugate
- Red dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Separation of human CD45 positive lymphocytes (red-filled) from CD45 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD45 (MEM-28) Alexa Fluor® 647 Monoclonal antibody (Product # MA5-18142) using a dilution of 4 µL reagent/100 µL of peripheral whole blood.
- Conjugate
- Red dye