Antibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [3]
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- Product number
- Q10069 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD45RA Monoclonal Antibody (MEM-56), Qdot™ 655
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Qdot™ Antibody (Ab) conjugates possess a bright fluorescence emission that makes them well suited for the detection of low-abundance extracellular proteins. Approximately the same size as R-phycoerythrin (R-PE) and compatible with existing organic fluorophore conjugates, Qdot™ Ab conjugates can be excited with any wavelength below their emission maximum, but are best excited by UV or violet light. The narrow, symmetric emission profiles of Qdot Ab conjugates allow for minimal compensation when using a single excitation source, and the very long stoke shifts enable better, more efficient multicolor assays using the 405 nm violet laser. Available in multiple colors for use in flow cytometry, these advantages make Qdot Ab conjugates powerful tools for antibody labeling and staining. Staining: Stain cells in any standard staining buffer, such as phosphate buffered saline (PBS) with 1% bovine serum albumin (BSA). We recommend analysis of cells within 18 hours of staining. If dilute reagent is used, dilute only the quantity of reagent to be used within one day. Qdot Ab conjugates may be mixed with other antibodies, but use the diluted conjugates on the day of dilution. Qdot Ab conjugates can be used for surface staining applications with most conventional sample preparation reagents, such as Cal-Lyse™ Lysing Solution and FIX & PERM™ reagents, with minimal effect on fluorescence. We have observed some batches of BD FACS™ Lysing Solution to interfere with Qdot Ab conjugate fluorescence. Each lot has been tested by flow cytometry using human peripheral blood leukocytes. The isotype control for this antibody is mouse IgG2a, Cat. No. Q10015. Instrument setup: Qdot Ab conjugates are excited optimally with UV or 405 nm light, although excitation can be obtained with any wavelength below the emission maximum of a given Qdot™ nanocrystal, such as with a 488-nm laser. Qdot Ab conjugates can be used on cytometers that do not have UV or violet excitation sources as long as they have appropriate emission filters. Make sure the cytometer has an appropriate emission filter for the Qdot Ab conjugate being used; alternate filters can be used as long as they capture the emission maximum, but filter width impacts spectral overlap corrections. And be sure to check for Qdot Ab conjugate emission in any channel that can capture nanocrystal emission off of other lasers on the cytometer. For Cat. No. Q10069: peak excitation 405 (488) nm/peak emission 655 nm; recommended filter 655/20 nm. Store reagents at 2-8°C in the dark. Do not freeze. Because Qdot nanocrystals are conjugated to biological materials, some loss of activity may be observed with prolonged storage. When stored as instructed, expires six months from date of receipt unless otherwise indicated on product label. Qdot Ab conjugates are photostable, and do not need to be protected from light. However, if using Qdot Ab conjugates in combination with conventional fluorochrome conjugated antibodies, minimize light exposure during handling, incubation with cells, and prior to analysis. The Qdot Ab conjugates contain cadmium and selenium in an inorganic crystalline form. Dispose of the material in compliance with all applicable local, state, and federal regulations for disposal of these classes of material. For more information on the composition of these materials, consult the Safety Data Sheets (SDSs).
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- MEM-56
- Vial size
- 100 µL
- Storage
- 4° C, store in dark
Submitted references Broadly reactive human CD4(+) T cells against Enterobacteriaceae are found in the naïve repertoire and are clonally expanded in the memory repertoire.
Deciphering and predicting CD4+ T cell immunodominance of influenza virus hemagglutinin.
T Cell Repertoire Maturation Induced by Persistent and Latent Viral Infection Is Insufficient to Induce Costimulation Blockade Resistant Organ Allograft Rejection in Mice.
Detailed characterization of human Mycobacterium tuberculosis specific HLA-E restricted CD8(+) T cells.
The Transcription Factor Hobit Identifies Human Cytotoxic CD4(+) T Cells.
Strain-specific Plasmodium falciparum multifunctional CD4(+) T cell cytokine expression in Malian children immunized with the FMP2.1/AS02A vaccine candidate.
Optimization of the cytokine secretion assay for human IL-2 in single and combination assays.
Cytokine diversity in the Th1-dominated human anti-influenza response caused by variable cytokine expression by Th1 cells, and a minor population of uncommitted IL-2+IFNγ- Thpp cells.
Ki-67 expression reveals strong, transient influenza specific CD4 T cell responses after adult vaccination.
CD8+ T cell immunity to 2009 pandemic and seasonal H1N1 influenza viruses.
Cassotta A, Goldstein JD, Durini G, Jarrossay D, Baggi Menozzi F, Venditti M, Russo A, Falcone M, Lanzavecchia A, Gagliardi MC, Latorre D, Sallusto F
European journal of immunology 2021 Mar;51(3):648-661
European journal of immunology 2021 Mar;51(3):648-661
Deciphering and predicting CD4+ T cell immunodominance of influenza virus hemagglutinin.
Cassotta A, Paparoditis P, Geiger R, Mettu RR, Landry SJ, Donati A, Benevento M, Foglierini M, Lewis DJM, Lanzavecchia A, Sallusto F
The Journal of experimental medicine 2020 Oct 5;217(10)
The Journal of experimental medicine 2020 Oct 5;217(10)
T Cell Repertoire Maturation Induced by Persistent and Latent Viral Infection Is Insufficient to Induce Costimulation Blockade Resistant Organ Allograft Rejection in Mice.
Espinosa JR, Mou D, Adams BW, DiBernardo LR, MacDonald AL, McRae M, Miller AN, Song M, Stempora LL, Wang J, Iwakoshi NN, Kirk AD
Frontiers in immunology 2018;9:1371
Frontiers in immunology 2018;9:1371
Detailed characterization of human Mycobacterium tuberculosis specific HLA-E restricted CD8(+) T cells.
Prezzemolo T, van Meijgaarden KE, Franken KLMC, Caccamo N, Dieli F, Ottenhoff THM, Joosten SA
European journal of immunology 2018 Feb;48(2):293-305
European journal of immunology 2018 Feb;48(2):293-305
The Transcription Factor Hobit Identifies Human Cytotoxic CD4(+) T Cells.
Oja AE, Vieira Braga FA, Remmerswaal EB, Kragten NA, Hertoghs KM, Zuo J, Moss PA, van Lier RA, van Gisbergen KP, Hombrink P
Frontiers in immunology 2017;8:325
Frontiers in immunology 2017;8:325
Strain-specific Plasmodium falciparum multifunctional CD4(+) T cell cytokine expression in Malian children immunized with the FMP2.1/AS02A vaccine candidate.
Graves SF, Kouriba B, Diarra I, Daou M, Niangaly A, Coulibaly D, Keita Y, Laurens MB, Berry AA, Vekemans J, Ripley Ballou W, Lanar DE, Dutta S, Gray Heppner D, Soisson L, Diggs CL, Thera MA, Doumbo OK, Plowe CV, Sztein MB, Lyke KE
Vaccine 2016 May 17;34(23):2546-55
Vaccine 2016 May 17;34(23):2546-55
Optimization of the cytokine secretion assay for human IL-2 in single and combination assays.
Deng N, Mosmann TR
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2015 Aug;87(8):777-83
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2015 Aug;87(8):777-83
Cytokine diversity in the Th1-dominated human anti-influenza response caused by variable cytokine expression by Th1 cells, and a minor population of uncommitted IL-2+IFNγ- Thpp cells.
Deng N, Weaver JM, Mosmann TR
PloS one 2014;9(5):e95986
PloS one 2014;9(5):e95986
Ki-67 expression reveals strong, transient influenza specific CD4 T cell responses after adult vaccination.
Li X, Miao H, Henn A, Topham DJ, Wu H, Zand MS, Mosmann TR
Vaccine 2012 Jun 29;30(31):4581-4
Vaccine 2012 Jun 29;30(31):4581-4
CD8+ T cell immunity to 2009 pandemic and seasonal H1N1 influenza viruses.
Scheible K, Zhang G, Baer J, Azadniv M, Lambert K, Pryhuber G, Treanor JJ, Topham DJ
Vaccine 2011 Mar 3;29(11):2159-68
Vaccine 2011 Mar 3;29(11):2159-68
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Qdot® 655 anti-human CD45RA conjugate 655/20 band pass filter
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL