Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- V2245 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- CD45RB Antibody
- Antibody type
- Monoclonal
- Description
- This highly specific CD45RB antibody is suitable for use in Flow cytometry/Immunofluorescence/Western blot/Immunohistochemistry applications with human samples.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- BRA-11
- Vial size
- 20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)
- Concentration
- 0.2 mg/ml, 1 mg/ml
- Storage
- The CD45RB antibody (with azide) can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.
Submitted references Repression of the PDCD2 gene by BCL6 and the implications for the pathogenesis of human B and T cell lymphomas.
Eosinophils from patients with asthma express higher levels of the pan-leucocyte receptor CD45 and the isoform CD45RO.
CD100 is associated with CD45 at the surface of human T lymphocytes. Role in T cell homotypic adhesion.
Baron BW, Zeleznik-Le N, Baron MJ, Theisler C, Huo D, Krasowski MD, Thirman MJ, Baron RM, Baron JM
Proceedings of the National Academy of Sciences of the United States of America 2007 May 1;104(18):7449-54
Proceedings of the National Academy of Sciences of the United States of America 2007 May 1;104(18):7449-54
Eosinophils from patients with asthma express higher levels of the pan-leucocyte receptor CD45 and the isoform CD45RO.
Blaylock MG, Lipworth BJ, Dempsey OJ, Duncan CJ, Lee DK, Lawrie A, Douglas JG, Walsh GM
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 2003 Jul;33(7):936-41
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology 2003 Jul;33(7):936-41
CD100 is associated with CD45 at the surface of human T lymphocytes. Role in T cell homotypic adhesion.
Herold C, Elhabazi A, Bismuth G, Bensussan A, Boumsell L
Journal of immunology (Baltimore, Md. : 1950) 1996 Dec 15;157(12):5262-8
Journal of immunology (Baltimore, Md. : 1950) 1996 Dec 15;157(12):5262-8
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of CD45RB antibody and human Raji lysate. Expected molecular weight: 147-220 kDa depending on glycosylation level.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of human tonsil stained with CD45RB antibody (BRA-11).
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC staining of FFPE human tonsil tissue with CD45RB antibody (clone BRA-11). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry staining of PFA-fixed human Raji cells with CD45RB antibody (clone BRA-11); Red=isotype control, Blue= CD45RB antibody.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE analysis of purified, BSA-free CD45RB antibody (clone BRA-11) as confirmation of integrity and purity.
