Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- V2399 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- CD45 Antibody
- Antibody type
- Monoclonal
- Antigen
- Stimulated human leukocytes were used as the immunogen for the CD45 antibody.
- Description
- Protein G purified antibody
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 135-4C5
- Vial size
- 20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)
- Storage
- CD45 antibody (with azide) can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
Submitted references Inflammatory bowel disease-associated interleukin-33 is preferentially expressed in ulceration-associated myofibroblasts.
Characterization of galectin-9-induced death of Jurkat T cells.
Tissue-factor-bearing microvesicles arise from lipid rafts and fuse with activated platelets to initiate coagulation.
Regulation of the tyrosine kinase-dependent adhesion pathway in human lymphocytes through CD45.
The role of CD44, CD45, CD45RO, CD46 and CD55 as potential anti-adhesion molecules involved in the binding of human tonsillar T cells to phorbol 12-myristate 13-acetate-differentiated U-937 cells.
Immunophenotypic differences between osteoclasts and macrophage polykaryons: immunohistological distinction and implications for osteoclast ontogeny and function.
Differential effects of anti-CD45 monoclonal antibody on human B cell proliferation: a monoclonal antibody recognizing a neuraminidase-sensitive epitope of the T200 molecule enhances anti-immunoglobulin-induced proliferation.
Sponheim J, Pollheimer J, Olsen T, Balogh J, Hammarström C, Loos T, Kasprzycka M, Sørensen DR, Nilsen HR, Küchler AM, Vatn MH, Haraldsen G
The American journal of pathology 2010 Dec;177(6):2804-15
The American journal of pathology 2010 Dec;177(6):2804-15
Characterization of galectin-9-induced death of Jurkat T cells.
Lu LH, Nakagawa R, Kashio Y, Ito A, Shoji H, Nishi N, Hirashima M, Yamauchi A, Nakamura T
Journal of biochemistry 2007 Feb;141(2):157-72
Journal of biochemistry 2007 Feb;141(2):157-72
Tissue-factor-bearing microvesicles arise from lipid rafts and fuse with activated platelets to initiate coagulation.
Del Conde I, Shrimpton CN, Thiagarajan P, López JA
Blood 2005 Sep 1;106(5):1604-11
Blood 2005 Sep 1;106(5):1604-11
Regulation of the tyrosine kinase-dependent adhesion pathway in human lymphocytes through CD45.
Wagner N, Engel P, Tedder TF
Journal of immunology (Baltimore, Md. : 1950) 1993 Jun 1;150(11):4887-99
Journal of immunology (Baltimore, Md. : 1950) 1993 Jun 1;150(11):4887-99
The role of CD44, CD45, CD45RO, CD46 and CD55 as potential anti-adhesion molecules involved in the binding of human tonsillar T cells to phorbol 12-myristate 13-acetate-differentiated U-937 cells.
King PD, Batchelor AH, Lawlor P, Katz DR
European journal of immunology 1990 Feb;20(2):363-8
European journal of immunology 1990 Feb;20(2):363-8
Immunophenotypic differences between osteoclasts and macrophage polykaryons: immunohistological distinction and implications for osteoclast ontogeny and function.
Athanasou NA, Quinn J
Journal of clinical pathology 1990 Dec;43(12):997-1003
Journal of clinical pathology 1990 Dec;43(12):997-1003
Differential effects of anti-CD45 monoclonal antibody on human B cell proliferation: a monoclonal antibody recognizing a neuraminidase-sensitive epitope of the T200 molecule enhances anti-immunoglobulin-induced proliferation.
Alsinet E, Inglés-Esteve J, Vilella R, Lozano F, Milá J, Rojo I, Martorell J, Vives J, Gayá A
European journal of immunology 1990 Dec;20(12):2801-4
European journal of immunology 1990 Dec;20(12):2801-4
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of human tonsil with CD45 antibody
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- SDS-PAGE Analysis of Purified, BSA-Free CD45 Antibody (clone 135-4C5). Confirmation of Integrity and Purity of the Antibody.