AM09001PU-N

antibody from OriGene

Targeting: PTPRC CD45, GP180, LCA, T200

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AM09001PU-N

Provider

OriGene

Product name

CD45 (PTPRC) mouse monoclonal antibody, clone 3G4, Purified

Antibody type

Monoclonal

Description

CD45 (PTPRC) mouse monoclonal antibody, clone 3G4, Purified

Host

Mouse

Conjugate

Unconjugated

Epitope

PTPRC

Isotype

IgG

Antibody clone number

3G4

Vial size

100 µl

Concentration

1.0 mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis on Jurkat cells: Cell lysates of Jurkat (20 ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human CD45 (1:2000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemistry: CD45 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Tonsil at 10 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Validation comment

IHC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Immunohistochemistry: CD45 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Thymus at 10 ug/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Validation comment

IHC