MHCD4505

antibody from Invitrogen Antibodies

Targeting: PTPRC CD45, GP180, LCA, T200

Provider product page for MHCD4505

Flow cytometry

Other assay

Antibody data

Antibody Data
Antigen structure
References [17]
Comments [0]
Validations
Flow cytometry [1]
Other assay [6]

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Product number: MHCD4505 - Provider product page
Provider: Invitrogen Antibodies
Product name: CD45 Monoclonal Antibody (HI30), APC
Antibody type: Monoclonal
Antigen: Other
Description: Allophycocyanin (APC) is a stable and highly soluble phycobiliprotein that provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing exceptional quantum yields and molar extinction coefficients.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: HI30
Vial size: 500 µL
Storage: 4° C, store in dark

PTPRC protein structure - MHCD4505 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 KD of L3MBTL1 Primes the Hematopoietic Potential of iPSCs (A) Endogenous L3MBTL1 expression was assessed in iPSCs at different time points during the mesodermal, hematopoietic, and erythroid differentiation using quantitative real-time PCR. The data represent the mean +- SD of the three independent experiments. (B) Strategy diagram. L3MBTL1 expression is efficiently knocked down as assessed by western blot assay in undifferentiated iPSCs. Tubulin served as the loading control. (C) KD of L3MBTL1 increases the expression of mesodermal-specific transcription factors while it decreases expression of key markers of endodermal and ectodermal lineages, as shown in L3MBTL1-KD undifferentiated iPSCs by qPCR compared to controls. Data indicate the relative expression level of the gene of interest, normalized by GAPDH . The reported fold changes have been calculated by DeltaDeltaCt analysis versus control cells. The data represent the mean +- SD of the three independent experiments. ** p < 0.01 by Student's t test. (D) KD of L3MBTL1 increases CFU capacity of iPSC-derived HSCs. Cells from day 10 hEBs were plated in methylcellulose and colonies were scored after 15 days. The data represent the mean +- SD of the three independent experiments. * p < 0.05 by Student's t test. (E) KD of L3MBTL1 promotes the emergence of early hemogenic precursor cells. EBs were harvested at day 10, prior to CD45 emergence, and analyzed by flow cytometry for CD31 and CD34 expression. Refer t

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 10.1371/journal.ppat.1009210.g001 Fig 1 Increased EPHA2 expression leads to higher EBV infection efficiency in cell lines. (A and B) EPHA2 mRNA was measured by RT-qPCR (A) and protein quantified by flow cytometry (B). (C) At 4 dpi, EBV transfer-infected cells were stained with CD45-APC antibody and analyzed by flow cytometry. (D-J) After EGF treatment or lentiviral overexpression of EPHA2 in AdAH cells, EPHA2 expression was measured by RT-qPCR (D and G) or flow cytometry (H) and EBV transfer-infection efficiency was evaluated by fluorescence microscopy (E and J) and flow cytometry (F and I). (K and L) AdAH cells were incubated with EPHA2 ligand ephrinA1 or anti-EPHA2 antibody, infected by transfer infection and infected epithelial cells were measured by flow cytometry (K) and fluorescence microscopy (L). (A), (C), (D), (F), (G), (I) and (K) represent means with SD from three independent experiments. RT-qPCR results in (A), (D) and (G) were normalized to GAPDH expression and then to Akata B cells, sample without EGF or native cells, respectively. (E), (J), (L) show representative images from three independent experiments. Scale in E and L: 400 mum. Scale in J: 200 mum.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: 10.1371/journal.ppat.1009210.g003 Fig 3 Despite comparable EPHA2 expression levels in organoids vs. epithelial cell lines there is no efficient EBV infection in human gastric organoids. (A) Scheme depicting B cell-mediated transfer infection of organoid-derived monolayers. (B) EBV transfer-infected organoid-derived monolayers were checked at 6 dpi by fluorescence microscopy. Scale: 200 mum. Representative image of at least three independent experiments. (C) At 6 dpi immunofluorescence was performed on EBV transfer-infected organoid-derived monolayers for epithelial marker Occludin, GFP-expressing EBV and lymphocyte marker CD45. DNA was counterstained with Hoechst. (I) depicts close-up of infected primary epithelial/organoid cell (GFP+, Occludin+ and CD45-). (II) depicts close-up of infected remaining B cells (GFP+, CD45+). Scale: 200 mum. Representative images of three independent experiments. (D) Flow cytometry gating strategy for evaluation of EBV infection efficiency. Left plot depicts FSC/SSC with gated cell population in P1. Middle plot depicts FSC/PI with gated viable cells in R1. Right plot depicts CD45-APC/EBV-GFP displaying localization of different cell populations. Q1: CD45+/GFP- = uninfected B cells, Q2: CD45+/GFP+ = infected B cells, Q3: CD45-/GFP- = uninfected epithelial cells and Q4: CD45-/GFP+ = infected epithelial cells. (E) At 4-6 dpi, EBV transfer-infected organoid-derived monolayers from different donors were analyzed for EBV infection rate by flow cytomet