Antibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [6]
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Validation data
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- Product number
- MHCD4517 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD45 Monoclonal Antibody (HI30), PE-Texas Red
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- The R-phycoerythrin (PE)-Texas Red conjugate permits simultaneous multicolor labeling and detection of multiple targets with excitation by a single excitation source-the 488 nm line of the argon-ion laser.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- HI30
- Vial size
- 500 µL
- Storage
- 4° C, store in dark
Submitted references Preventive efficacy of a tenofovir alafenamide fumarate nanofluidic implant in SHIV-challenged nonhuman primates.
Immunoprophylactic and immunotherapeutic control of hormone receptor-positive breast cancer.
Human Tumor-Associated Macrophage and Monocyte Transcriptional Landscapes Reveal Cancer-Specific Reprogramming, Biomarkers, and Therapeutic Targets.
Dynamic change in natural killer cell type in the human ocular mucosa in situ as means of immune evasion by adenovirus infection.
Defining human dendritic cell progenitors by multiparametric flow cytometry.
Coronary artery endothelial cells and microparticles increase expression of VCAM-1 in myocardial infarction.
Interleukin-17-producing innate lymphoid cells and the NLRP3 inflammasome facilitate obesity-associated airway hyperreactivity.
Pons-Faudoa FP, Sizovs A, Shelton KA, Momin Z, Niles JA, Bushman LR, Xu J, Chua CYX, Nichols JE, Demaria S, Ittmann MM, Hawkins T, Rooney JF, Marzinke MA, Kimata JT, Anderson PL, Nehete PN, Arduino RC, Ferrari M, Sastry KJ, Grattoni A
Advanced therapeutics 2021 Mar;4(3)
Advanced therapeutics 2021 Mar;4(3)
Immunoprophylactic and immunotherapeutic control of hormone receptor-positive breast cancer.
Buqué A, Bloy N, Perez-Lanzón M, Iribarren K, Humeau J, Pol JG, Levesque S, Mondragon L, Yamazaki T, Sato A, Aranda F, Durand S, Boissonnas A, Fucikova J, Senovilla L, Enot D, Hensler M, Kremer M, Stoll G, Hu Y, Massa C, Formenti SC, Seliger B, Elemento O, Spisek R, André F, Zitvogel L, Delaloge S, Kroemer G, Galluzzi L
Nature communications 2020 Jul 30;11(1):3819
Nature communications 2020 Jul 30;11(1):3819
Human Tumor-Associated Macrophage and Monocyte Transcriptional Landscapes Reveal Cancer-Specific Reprogramming, Biomarkers, and Therapeutic Targets.
Cassetta L, Fragkogianni S, Sims AH, Swierczak A, Forrester LM, Zhang H, Soong DYH, Cotechini T, Anur P, Lin EY, Fidanza A, Lopez-Yrigoyen M, Millar MR, Urman A, Ai Z, Spellman PT, Hwang ES, Dixon JM, Wiechmann L, Coussens LM, Smith HO, Pollard JW
Cancer cell 2019 Apr 15;35(4):588-602.e10
Cancer cell 2019 Apr 15;35(4):588-602.e10
Dynamic change in natural killer cell type in the human ocular mucosa in situ as means of immune evasion by adenovirus infection.
Yawata N, Selva KJ, Liu YC, Tan KP, Lee AW, Siak J, Lan W, Vania M, Arundhati A, Tong L, Li J, Mehta JS, Yawata M
Mucosal immunology 2016 Jan;9(1):159-70
Mucosal immunology 2016 Jan;9(1):159-70
Defining human dendritic cell progenitors by multiparametric flow cytometry.
Breton G, Lee J, Liu K, Nussenzweig MC
Nature protocols 2015 Sep;10(9):1407-22
Nature protocols 2015 Sep;10(9):1407-22
Coronary artery endothelial cells and microparticles increase expression of VCAM-1 in myocardial infarction.
Radecke CE, Warrick AE, Singh GD, Rogers JH, Simon SI, Armstrong EJ
Thrombosis and haemostasis 2015 Mar;113(3):605-16
Thrombosis and haemostasis 2015 Mar;113(3):605-16
Interleukin-17-producing innate lymphoid cells and the NLRP3 inflammasome facilitate obesity-associated airway hyperreactivity.
Kim HY, Lee HJ, Chang YJ, Pichavant M, Shore SA, Fitzgerald KA, Iwakura Y, Israel E, Bolger K, Faul J, DeKruyff RH, Umetsu DT
Nature medicine 2014 Jan;20(1):54-61
Nature medicine 2014 Jan;20(1):54-61
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Ammonium chloride lysed whole blood was stained with mouse anti human CD45 PE-Texas Red (Product # MHCD4517), washed, and analyzed on a flow cytometer with a 488nm excitation source.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 3 IL-1beta production and M1 macrophages are increased in the lungs of obese mice (a-f). Total RNA was extracted from the lung and adipose tissue for qRT-PCR. Fold induction of Il1b ( a,d ), Il6 ( b,e ), and Il23 ( c,f ) were calculated based on GAPDH expression. * p
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 2 HFD induced AHR requires the presence of IL-17A a . Cells were isolated from lungs of mice fed on normal chow or on the HFD, and supernatants were analyzed for IL-17A by ELISA. b. Comparison of bodyweight gain between WT and Il17 -/- mice on either chow or HFD. NS, WT mice on HFD vs Il17 -/- mice on HFD (Student's t-test). c. AHR was measured using mice represented in Fig. 2b. Graph represents the changes in lung resistance (R L ). * p
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 6 Treatment of obese mice with anti-IL-1, prevents AHR a. Schedule of feeding and anti-IL1R antagonist treatment. To block IL-1beta (and IL-1alpha), obese mice were treated with IL1R antagonist (50 mg/kg) subcutaneously for 7 days before measuring AHR. b . AHR was measured 24hr after last IL1R antagonist treatment. Graph represents the changes in lung resistance (R L ). *** p