MHCD4529

antibody from Invitrogen Antibodies

Targeting: PTPRC CD45, GP180, LCA, T200

Flow cytometry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MHCD4529

Provider

Invitrogen Antibodies

Product name

CD45 Monoclonal Antibody (HI30), Alexa Fluor™ 700

Antibody type

Monoclonal

Antigen

Other

Description

The Alexa Fluor® 700 dye conjugate provides an excellent spectral match to the common long-wavelength excitation sources, with a high extinction coefficient.

Reactivity

Human

Host

Mouse

Conjugate

Near infrared dye

Isotype

IgG

Antibody clone number

HI30

Vial size

500 µL

Storage

4° C, store in dark

References

Submitted references

The genetic basis and cell of origin of mixed phenotype acute leukaemia.

Alexander TB, Gu Z, Iacobucci I, Dickerson K, Choi JK, Xu B, Payne-Turner D, Yoshihara H, Loh ML, Horan J, Buldini B, Basso G, Elitzur S, de Haas V, Zwaan CM, Yeoh A, Reinhardt D, Tomizawa D, Kiyokawa N, Lammens T, De Moerloose B, Catchpoole D, Hori H, Moorman A, Moore AS, Hrusak O, Meshinchi S, Orgel E, Devidas M, Borowitz M, Wood B, Heerema NA, Carrol A, Yang YL, Smith MA, Davidsen TM, Hermida LC, Gesuwan P, Marra MA, Ma Y, Mungall AJ, Moore RA, Jones SJM, Valentine M, Janke LJ, Rubnitz JE, Pui CH, Ding L, Liu Y, Zhang J, Nichols KE, Downing JR, Cao X, Shi L, Pounds S, Newman S, Pei D, Guidry Auvil JM, Gerhard DS, Hunger SP, Inaba H, Mullighan CG
Nature 2018 Oct;562(7727):373-379

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Chronic stress is associated with reduced circulating hematopoietic progenitor cell number: A maternal caregiving model.

Aschbacher K, Milush JM, Gilbert A, Almeida C, Sinclair E, Epling L, Grenon SM, Marco EJ, Puterman E, Epel E
Brain, behavior, and immunity 2017 Jan;59:245-252

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Dynamic change in natural killer cell type in the human ocular mucosa in situ as means of immune evasion by adenovirus infection.

Yawata N, Selva KJ, Liu YC, Tan KP, Lee AW, Siak J, Lan W, Vania M, Arundhati A, Tong L, Li J, Mehta JS, Yawata M
Mucosal immunology 2016 Jan;9(1):159-70

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Endovascular biopsy: Strategy for analyzing gene expression profiles of individual endothelial cells obtained from human vessels(✩).

Sun Z, Lawson DA, Sinclair E, Wang CY, Lai MD, Hetts SW, Higashida RT, Dowd CF, Halbach VV, Werb Z, Su H, Cooke DL
Biotechnology reports (Amsterdam, Netherlands) 2015 Sep;7:157-165

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OMIP-012: Phenotypic and numeric determination of human leukocyte reconstitution in humanized mice.

Long BR, Stoddart CA
Cytometry. Part A : the journal of the International Society for Analytical Cytology 2012 Aug;81(8):646-8

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Conjugate

Near infrared dye

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Conjugate

Near infrared dye

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Conjugate

Near infrared dye

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Conjugate

Near infrared dye

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 2 FACS gating strategy for EC collection. Seven cell surface markers and one viability marker were used to gate the EC candidates. Cells were first gated to exclude debris, doublets and dead cells identified by positive Aqua Amine stain. After gating on the viable single cells, the leukocytes (CD45 + ), macrophages (CD11b + ) and platelets (CD42b + ) were eliminated. EC candidates were first selected by CD31 and CD34, and then CD105 and CD146.

Conjugate

Near infrared dye