MHCD4530TR

antibody from Invitrogen Antibodies

Targeting: PTPRC CD45, GP180, LCA, T200

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MHCD4530TR

Provider

Invitrogen Antibodies

Product name

CD45 Monoclonal Antibody (HI30), Pacific Orange™

Antibody type

Monoclonal

Antigen

Other

Description

Our Pacific Orange™ dye is optimally excited by the violet laser, and it is recommended that a 575, 585 or 600 nm band pass filter be used for optimal detection. Pacific Blue™ and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm and 551 nm, respectively, facilitating two-color analysis.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

HI30

Vial size

25 Tests

Storage

4° C, store in dark

References

Submitted references

Improving NK cell function in multiple myeloma with NKTR-255, a novel polymer-conjugated human IL-15.

Fernandez RA, Mayoral JE, Pierre-Louis L, Yao Y, Xu Y, Mu S, Martinez-Lopez J, Primo D, Miyazaki T, Prabhala R, Anderson KC, Overwijk WW, Munshi NC, Fulciniti M
Blood advances 2023 Jan 10;7(1):9-19

---

Comparison of Two Strategies to Generate Antigen-Specific Human Monoclonal Antibodies: Which Method to Choose for Which Purpose?

Ehlers AM, den Hartog Jager CF, Kardol-Hoefnagel T, Katsburg MMD, Knulst AC, Otten HG
Frontiers in immunology 2021;12:660037

---

Enhancer Hijacking Drives Oncogenic BCL11B Expression in Lineage-Ambiguous Stem Cell Leukemia.

Montefiori LE, Bendig S, Gu Z, Chen X, Pölönen P, Ma X, Murison A, Zeng A, Garcia-Prat L, Dickerson K, Iacobucci I, Abdelhamed S, Hiltenbrand R, Mead PE, Mehr CM, Xu B, Cheng Z, Chang TC, Westover T, Ma J, Stengel A, Kimura S, Qu C, Valentine MB, Rashkovan M, Luger S, Litzow MR, Rowe JM, den Boer ML, Wang V, Yin J, Kornblau SM, Hunger SP, Loh ML, Pui CH, Yang W, Crews KR, Roberts KG, Yang JJ, Relling MV, Evans WE, Stock W, Paietta EM, Ferrando AA, Zhang J, Kern W, Haferlach T, Wu G, Dick JE, Klco JM, Haferlach C, Mullighan CG
Cancer discovery 2021 Nov;11(11):2846-2867

---

Loss of Optineurin Drives Cancer Immune Evasion via Palmitoylation-Dependent IFNGR1 Lysosomal Sorting and Degradation.

Du W, Hua F, Li X, Zhang J, Li S, Wang W, Zhou J, Wang W, Liao P, Yan Y, Li G, Wei S, Grove S, Vatan L, Zgodziński W, Majewski M, Wallner G, Chen H, Kryczek I, Fang JY, Zou W
Cancer discovery 2021 Jul;11(7):1826-1843

---

Integration of T helper and BCR signals governs enhanced plasma cell differentiation of memory B cells by regulation of CD45 phosphatase activity.

Szodoray P, Andersen TK, Heinzelbecker J, Imbery JF, Huszthy PC, Stanford SM, Bogen B, Landsverk OB, Bottini N, Tveita A, Munthe LA, Nakken B
Cell reports 2021 Aug 10;36(6):109525

---

Maturation-associated gene expression profiles during normal human bone marrow erythropoiesis.

Mello FV, Land MGP, Costa ES, Teodósio C, Sanchez ML, Bárcena P, Peres RT, Pedreira CE, Alves LR, Orfao A
Cell death discovery 2019;5:69

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Scalable high-throughput acoustophoresis in arrayed plastic microchannels.

Dubay R, Lissandrello C, Swierk P, Moore N, Doty D, Fiering J
Biomicrofluidics 2019 May;13(3):034105

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Influence of nasal polyp tissue on the differentiation and activation of T lymphocytes in a co-culture system.

Ickrath P, Scherzad A, Kleinsasser N, Ginzkey C, Hagen R, Hackenberg S
Biomedical reports 2019 Feb;10(2):119-126

---

Optimization and testing of dried antibody tube: The EuroFlow LST and PIDOT tubes as examples.

van der Velden VHJ, Flores-Montero J, Perez-Andres M, Martin-Ayuso M, Crespo O, Blanco E, Kalina T, Philippé J, Bonroy C, de Bie M, Te Marvelde J, Teodosio C, Corral Mateos A, Kanderová V, van der Burg M, Van Hoof D, van Dongen JJM, Orfao A
Journal of immunological methods 2019 Dec;475:112287

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Targeting of colony-stimulating factor 1 receptor (CSF1R) in the CLL microenvironment yields antineoplastic activity in primary patient samples.

Edwards V DK, Sweeney DT, Ho H, Eide CA, Rofelty A, Agarwal A, Liu SQ, Danilov AV, Lee P, Chantry D, McWeeney SK, Druker BJ, Tyner JW, Spurgeon SE, Loriaux MM
Oncotarget 2018 May 15;9(37):24576-24589

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Immunophenotyping and transcriptional profiling of in vitro cultured human adipose tissue derived stem cells.

Mieczkowska A, Schumacher A, Filipowicz N, Wardowska A, Zieliński M, Madanecki P, Nowicka E, Langa P, Deptuła M, Zieliński J, Kondej K, Renkielska A, Buckley PG, Crossman DK, Crowley MR, Czupryn A, Mucha P, Sachadyn P, Janus Ł, Skowron P, Rodziewicz-Motowidło S, Cichorek M, Pikuła M, Piotrowski A
Scientific reports 2018 Jul 27;8(1):11339

---

Immunodeficiency in Bloom's Syndrome.

Schoenaker MHD, Henriet SS, Zonderland J, van Deuren M, Pan-Hammarström Q, Posthumus-van Sluijs SJ, Pico-Knijnenburg I, Weemaes CMR, IJspeert H
Journal of clinical immunology 2018 Jan;38(1):35-44

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Accumulation of CD69+ tissue‑resident memory T cells in the nasal polyps of patients with chronic rhinosinusitis.

Ickrath P, Kleinsasser N, Ding X, Ginzkey C, Beyersdorf N, Hagen R, Kerkau T, Hackenberg S
International journal of molecular medicine 2018 Aug;42(2):1116-1124

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Genetic defects in PI3Kδ affect B-cell differentiation and maturation leading to hypogammaglobulineamia and recurrent infections.

Wentink M, Dalm V, Lankester AC, van Schouwenburg PA, Schölvinck L, Kalina T, Zachova R, Sediva A, Lambeck A, Pico-Knijnenburg I, van Dongen JJ, Pac M, Bernatowska E, van Hagen M, Driessen G, van der Burg M
Clinical immunology (Orlando, Fla.) 2017 Mar;176:77-86

---

The IgE and basophil responses to Ara h 2 and Ara h 6 are good predictors of peanut allergy in children.

van Erp FC, Knol EF, Pontoppidan B, Meijer Y, van der Ent CK, Knulst AC
The Journal of allergy and clinical immunology 2017 Jan;139(1):358-360.e8

---

Dynamic change in natural killer cell type in the human ocular mucosa in situ as means of immune evasion by adenovirus infection.

Yawata N, Selva KJ, Liu YC, Tan KP, Lee AW, Siak J, Lan W, Vania M, Arundhati A, Tong L, Li J, Mehta JS, Yawata M
Mucosal immunology 2016 Jan;9(1):159-70

---

Plasmacytoid dendritic cells suppress HIV-1 replication but contribute to HIV-1 induced immunopathogenesis in humanized mice.

Li G, Cheng M, Nunoya J, Cheng L, Guo H, Yu H, Liu YJ, Su L, Zhang L
PLoS pathogens 2014 Jul;10(7):e1004291

---

Immune response to JC virus T antigen in patients with and without colorectal neoplasia.

Butcher LD, Garcia M, Arnold M, Ueno H, Goel A, Boland CR
Gut microbes 2014 Jul 1;5(4):468-75

---

Human plasmacytoid dendritic cells regulate IFN-α production through activation-induced splicing of IL-18Rα.

Chao Y, Kaliaperumal N, Chretien AS, Tang S, Lee B, Poidinger M, Fairhurst AM, Connolly JE
Journal of leukocyte biology 2014 Dec;96(6):1037-46

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Characterization of human antiviral adaptive immune responses during hepatotropic virus infection in HLA-transgenic human immune system mice.

Billerbeck E, Horwitz JA, Labitt RN, Donovan BM, Vega K, Budell WC, Koo GC, Rice CM, Ploss A
Journal of immunology (Baltimore, Md. : 1950) 2013 Aug 15;191(4):1753-64

---

In vitro immunomodulation of a whole blood IFN-γ release assay enhances T cell responses in subjects with latent tuberculosis infection.

Gaur RL, Suhosk MM, Banaei N
PloS one 2012;7(10):e48027

---

EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes.

van Dongen JJ, Lhermitte L, Böttcher S, Almeida J, van der Velden VH, Flores-Montero J, Rawstron A, Asnafi V, Lécrevisse Q, Lucio P, Mejstrikova E, Szczepański T, Kalina T, de Tute R, Brüggemann M, Sedek L, Cullen M, Langerak AW, Mendonça A, Macintyre E, Martin-Ayuso M, Hrusak O, Vidriales MB, Orfao A, EuroFlow Consortium (EU-FP6, LSHB-CT-2006-018708)
Leukemia 2012 Sep;26(9):1908-75

---

FcRL5 as a target of antibody-drug conjugates for the treatment of multiple myeloma.

Elkins K, Zheng B, Go M, Slaga D, Du C, Scales SJ, Yu SF, McBride J, de Tute R, Rawstron A, Jack AS, Ebens A, Polson AG
Molecular cancer therapeutics 2012 Oct;11(10):2222-32

---

Rapid ex vivo isolation and long-term culture of human Th17 cells.

Streeck H, Cohen KW, Jolin JS, Brockman MA, Meier A, Power KA, Waring MT, Alter G, Altfeld M
Journal of immunological methods 2008 Apr 20;333(1-2):115-25

---

Mobilization of bone marrow-derived stem cells after myocardial infarction and left ventricular function.

Leone AM, Rutella S, Bonanno G, Abbate A, Rebuzzi AG, Giovannini S, Lombardi M, Galiuto L, Liuzzo G, Andreotti F, Lanza GA, Contemi AM, Leone G, Crea F
European heart journal 2005 Jun;26(12):1196-204

---

Analysis of CD10+ hairy cell leukemia.

Jasionowski TM, Hartung L, Greenwood JH, Perkins SL, Bahler DW
American journal of clinical pathology 2003 Aug;120(2):228-35

---

Prospective isolation of human clonogenic common myeloid progenitors.

Manz MG, Miyamoto T, Akashi K, Weissman IL
Proceedings of the National Academy of Sciences of the United States of America 2002 Sep 3;99(18):11872-7

---

Precise CD4 T-cell counting using red diode laser excitation: for richer, for poorer.

Janossy G, Jani IV, Kahan M, Barnett D, Mandy F, Shapiro H
Cytometry 2002 Apr 15;50(2):78-85

---

Short-term, serum-free, static culture of cord blood-derived CD34+ cells: effects of FLT3-L and MIP-1alpha on in vitro expansion of hematopoietic progenitor cells.

Capmany G, Querol S, Cancelas JA, García J
Haematologica 1999 Aug;84(8):675-82

---

Illegitimate transcription: its use for studying genetic abnormalities in lymphoblastoid cells from patients with Glanzmann thrombasthenia.

Negrier C, Vinciguerra C, Attali O, Grenier C, Larcher ME, Dechavanne M
British journal of haematology 1998 Jan;100(1):33-9

---

Illegitimate transcription: its use for studying genetic abnormalities in lymphoblastoid cells from patients with Glanzmann thrombasthenia.

Negrier C, Vinciguerra C, Attali O, Grenier C, Larcher ME, Dechavanne M
British journal of haematology 1998 Jan;100(1):33-9

---

Growth of human hematopoietic cells in immunodeficient mice conditioned with cyclophosphamide and busulfan.

Basch RS, Quito FL, Beh J, Hirst JA
Stem cells (Dayton, Ohio) 1997;15(4):314-23

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human PBMCs stained with: CD45 Pacific Orange™ (Product # MHCD4530TR), CD4 PerCP-Cy®5.5 (Product # A15858), CD20 APC, CD14 APC-Cy®7 (Product # A15453), CD19 Pacific Green™ (Product # C11210), CD3 Alexa Fluor® 700 (CD0329), HLA-DR PE-Cy®7 (Product # A18558), CD8 Pacific Blue™ (Product # MHCD0828), CD33 FITC (Product # A16185), and CD11c PE (Product # A18674) using the Attune® NxT Acoustic Focusing Cytometer with 405 nm excitation and 440/50 emission filter (Pacific Blue™), 512/25 emission filter (Pacific Green™), and 603/48 emission filter (Pacific Orange™); 488 nm excitation and 530/30 emission filter (FITC) and 695/50 emission filter (PerCP-Cy®5.5); 561 nm excitation and 585/16 emission filter (PE) and 780/60 emission filter (PE-Cy®7); 637 nm excitation and 660/20 emission filter (APC), 720/30 emission filter (Alexa Fluor®700), and 780/60 emission filter (APC-Cy®7). Within the CD3 negative CD45 positive gate, B cells can be identified based on expression of CD19 and CD20. Conventional dendritic cells in peripheral blood are generally negative for T and B cell lineage markers and co-express the integrin CD11c and HLA-DR. Values noted are a percent of the parent CD19 negative CD20 negative gate (top value, no parenthesis) or percent of FSC/SSC lymphocyte gate (bottom value, in parentheses).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis using a CD45 monoclonal antibody (Product # MHCD4530).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human PBMCs stained with: CD45 Pacific Orange™ (Product # MHCD4530TR), CD4 PerCP-Cy®5.5 (Product # A15858), CD20 APC, CD14 APC-Cy®7 (Product # A15453), CD19 Pacific Green™ (Product # C11210), CD3 Alexa Fluor® 700 (CD0329), HLA-DR PE-Cy®7 (Product # A18558), CD8 Pacific Blue™ (Product # MHCD0828), CD33 FITC (Product # A16185), and CD11c PE (Product # A18674) using the Attune® NxT Acoustic Focusing Cytometer with 405 nm excitation and 440/50 emission filter (Pacific Blue™), 512/25 emission filter (Pacific Green™), and 603/48 emission filter (Pacific Orange™); 488 nm excitation and 530/30 emission filter (FITC) and 695/50 emission filter (PerCP-Cy®5.5); 561 nm excitation and 585/16 emission filter (PE) and 780/60 emission filter (PE-Cy®7); 637 nm excitation and 660/20 emission filter (APC), 720/30 emission filter (Alexa Fluor®700), and 780/60 emission filter (APC-Cy®7). Within the CD3 negative CD45 positive gate, B cells can be identified based on expression of CD19 and CD20. Conventional dendritic cells in peripheral blood are generally negative for T and B cell lineage markers and co-express the integrin CD11c and HLA-DR. Values noted are a percent of the parent CD19 negative CD20 negative gate (top value, no parenthesis) or percent of FSC/SSC lymphocyte gate (bottom value, in parentheses).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis using a CD45 monoclonal antibody (Product # MHCD4530).