MHCD4531

antibody from Invitrogen Antibodies

Targeting: PTPRC CD45, GP180, LCA, T200

Provider product page for MHCD4531

Flow cytometry

Other assay

Antibody data

Antibody Data
Antigen structure
References [12]
Comments [0]
Validations
Flow cytometry [2]
Other assay [2]

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Product number: MHCD4531 - Provider product page
Provider: Invitrogen Antibodies
Product name: CD45 Monoclonal Antibody (HI30), PerCP
Antibody type: Monoclonal
Antigen: Other
Description: PerCP conjugates are excited by the 488 nm line of the argon-ion laser and have a large Stokes shift, providing minimal spectral overlap into the R-PE (phycoerythrin) channel.
Reactivity: Human
Host: Mouse
Conjugate: Red dye
Isotype: IgG
Antibody clone number: HI30
Vial size: 500 µL
Storage: 4° C, store in dark

PTPRC protein structure - MHCD4531 shown in red.

Submitted references Mucosal-associated invariant T cell responses differ by sex in COVID-19.

Yu C, Littleton S, Giroux NS, Mathew R, Ding S, Kalnitsky J, Yang Y, Petzold E, Chung HA, Rivera GO, Rotstein T, Xi R, Ko ER, Tsalik EL, Sempowski GD, Denny TN, Burke TW, McClain MT, Woods CW, Shen X, Saban DR
Med (New York, N.Y.) 2021 Jun 11;2(6):755-772.e5

Differentiation Potential of Early- and Late-Passage Adipose-Derived Mesenchymal Stem Cells Cultured under Hypoxia and Normoxia.

Zhao AG, Shah K, Freitag J, Cromer B, Sumer H
Stem cells international 2020;2020:8898221

Osteopontin/secreted phosphoprotein-1 behaves as a molecular brake regulating the neuroinflammatory response to chronic viral infection.

Mahmud FJ, Du Y, Greif E, Boucher T, Dannals RF, Mathews WB, Pomper MG, Sysa-Shah P, Metcalf Pate KA, Lyons C, Carlson B, Chacona M, Brown AM
Journal of neuroinflammation 2020 Sep 17;17(1):273

The Presence of Survivin on B Cells from Myasthenia Gravis Patients and the Potential of an Antibody to a Modified Survivin Peptide to Alleviate Weakness in an Animal Model.

Zhang X, Ciesielski M, Fenstermaker RA, Kaminski HJ, Kusner LL
Journal of immunology (Baltimore, Md. : 1950) 2020 Oct 1;205(7):1743-1751

Leukocyte-Derived Extracellular Vesicles in Blood with and without EpCAM Enrichment.

Nanou A, Zeune LL, Terstappen LWMM
Cells 2019 Aug 20;8(8)

Scanning Electron Microscopy of Circulating Tumor Cells and Tumor-Derived Extracellular Vesicles.

Nanou A, Crespo M, Flohr P, De Bono JS, Terstappen LWMM
Cancers 2018 Oct 31;10(11)

Toward a real liquid biopsy in metastatic breast and prostate cancer: Diagnostic LeukApheresis increases CTC yields in a European prospective multicenter study (CTCTrap).

Andree KC, Mentink A, Zeune LL, Terstappen LWMM, Stoecklein NH, Neves RP, Driemel C, Lampignano R, Yang L, Neubauer H, Fehm T, Fischer JC, Rossi E, Manicone M, Basso U, Marson P, Zamarchi R, Loriot Y, Lapierre V, Faugeroux V, Oulhen M, Farace F, Fowler G, Sousa Fontes M, Ebbs B, Lambros M, Crespo M, Flohr P, de Bono JS
International journal of cancer 2018 Nov 15;143(10):2584-2591

Use of a novel antigen expressing system to study the Salmonella enterica serovar Typhi protein recognition by T cells.

Salerno-Gonçalves R, Tettelin H, Lou D, Steiner S, Rezwanul T, Guo Q, Picking WD, Nene V, Sztein MB
PLoS neglected tropical diseases 2017 Sep;11(9):e0005912

CD40 Expression in Fibrocytes Is Induced by TSH: Potential Synergistic Immune Activation.

Mester T, Raychaudhuri N, Gillespie EF, Chen H, Smith TJ, Douglas RS
PloS one 2016;11(9):e0162994

Thyrotropin receptor and CD40 mediate interleukin-8 expression in fibrocytes: implications for thyroid-associated ophthalmopathy (an American Ophthalmological Society thesis).

Douglas RS, Mester T, Ginter A, Kim DS
Transactions of the American Ophthalmological Society 2014;112:26-37

Teprotumumab, an IGF-1R blocking monoclonal antibody inhibits TSH and IGF-1 action in fibrocytes.

Chen H, Mester T, Raychaudhuri N, Kauh CY, Gupta S, Smith TJ, Douglas RS
The Journal of clinical endocrinology and metabolism 2014 Sep;99(9):E1635-40

Accurate quantitation of D+ fetomaternal hemorrhage by flow cytometry using a novel reagent to eliminate granulocytes from analysis.

Kumpel B, Hazell M, Guest A, Dixey J, Mushens R, Bishop D, Wreford-Bush T, Lee E
Transfusion 2014 May;54(5):1305-16

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Human peripheral blood lymphocytes were stained with PerCP conjugated anti-human CD45 monoclonal antibody (Product # MHCD4531). The negative profile represents unstained cells.
Conjugate: Red dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Human peripheral blood lymphocytes were stained with PerCP conjugated anti-human CD45 monoclonal antibody (Product # MHCD4531). The negative profile represents unstained cells.
Conjugate: Red dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL
Conjugate: Red dye

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Flow cytometry of CD cell surface markers for cells cultured under hypoxia and normoxia. The positive CD markers for MSCs as detected by the fluorescent antibodies anti-CD73 FITC, anti-CD105 PE, and anti-CD90 PE Cy7. The negative markers of MSCs were detected using anti-CD14 FITC, anti-CD45 PerCP, anti-CD34-R-PE, and anti-CD19 PE-Cy7 antibodies. Unstained cell for each condition was used as negative controls.
Conjugate: Red dye