Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Other assay [2]
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- Product number
- MHCD45RO01 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD45RO Monoclonal Antibody (UCHL1), FITC
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Commonly used, FITC conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Green dye
- Isotype
- IgG
- Antibody clone number
- UCHL1
- Vial size
- 500 µL
- Storage
- 4° C, store in dark
Submitted references Long-term in vitro persistence of magnetic properties after magnetic bead-based cell separation of T cells.
Interferon-alpha and -beta inhibit the in vitro differentiation of immunocompetent human dendritic cells from CD14(+) precursors.
Laghmouchi A, Hoogstraten C, Falkenburg JHF, Jedema I
Scandinavian journal of immunology 2020 Sep;92(3):e12924
Scandinavian journal of immunology 2020 Sep;92(3):e12924
Interferon-alpha and -beta inhibit the in vitro differentiation of immunocompetent human dendritic cells from CD14(+) precursors.
McRae BL, Nagai T, Semnani RT, van Seventer JM, van Seventer GA
Blood 2000 Jul 1;96(1):210-7
Blood 2000 Jul 1;96(1):210-7
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 1 Positively selected memory T cells undergoing no or limited proliferation retain magnetic properties. A, Dot plots of representative (from nine experiments in total) viable positively selected (CD45RO pos ) memory T cells and control, viable untouched isolated (CD45RA neg ) memory T cells at day 0. The gating was initially performed on lymphocytes using forward and sideward scatter, and then, cells were plotted for CD45RO and CD45RA. B, Positively selected memory T cells were cultured for 2 wk under different stimulation conditions (representative dotplots (from n = 3) of viable cells are shown, starting from left: without stimulation (cytokines only) resulting in no/minimal PKH dilution (no/minimal proliferation (cytokines only)); partial stimulation inducing proliferation of a portion of T cells (allo-reactive T cell response), a-specific stimulation inducing proliferation of almost all cells (PHA stimulation)). The gating on the lymphocytes was done using forward and sideward scatter, followed by plotting for CD3 and PKH to track the cell division. C, Both positively selected and untouched isolated memory T cells were applied after the 2 wk of in vitro culture onto MACS columns without additional magnetic bead labeling. Quantification was performed on viable cells, the cell frequencies in the flow-through fractions (grey squares) and the column-retained fractions (black circles) are presented as percentages of total viable cells applied onto the separation columns
- Conjugate
- Green dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 3 Residual magnetic properties disturb purities in repetitive isolation rounds. A, Representative (of n = 3) dotplots of viable cells are shown for allo-reactive T cell responses generated from positively selected memory T cells or untouched memory T cells (the quadrants were set based on the unstained samples). The gating of lymphocytes was based on the forward and sideward scatter after which they were plotted for CD3 and CD137 expression. After 2 wk in vitro culture of the two-differential isolated memory T cell populations, the cells were restimulated with HLA-mismatched EBV-LCL. CD137 (activation marker) staining was performed 24 h after the restimulation. To isolate the allo-reactive (activated) T cells, staining with CD137-APC and then indirect isolation with anti-APC magnetic beads was performed. After application on the separation columns, both the column-retained fractions and the flow-through fractions were analyzed for CD137 expression to assess the purity of the allo-reactive (activated/CD137 pos ) T cell isolation. B, Scatter plot with the quantification of three independent experiments shows the percentages of CD137 pos cells (black circles) and CD137 neg cells (grey squares) of total for both the CD137 negative fraction and the CD137 positive fraction. The experiment was repeated three times using three different donors. The statistical analysis was performed with t test in Prism 8 ( P -value: *
- Conjugate
- Green dye