Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- FAB114R-025UG - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CD45 Alexa Fluor® 647-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse CD45. Recognizes all CD45 isoforms as well as the known mouse CD45 alloantigens (CD45-1/Ly 5a and CD45-2/Ly 5b) (15).
- Reactivity
- Mouse
- Host
- Rat
- Conjugate
- Red dye
- Isotype
- IgG
- Antibody clone number
- 30-F11
- Vial size
- 25 ug
- Concentration
- 0.2 mg/ml
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Single-cell transcriptomes of the regenerating intestine reveal a revival stem cell.
Ayyaz A, Kumar S, Sangiorgi B, Ghoshal B, Gosio J, Ouladan S, Fink M, Barutcu S, Trcka D, Shen J, Chan K, Wrana JL, Gregorieff A
Nature 2019 May;569(7754):121-125
Nature 2019 May;569(7754):121-125
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CD45 in Mouse Bone Marrow Cells by Flow Cytometry. Mouse bone marrow cells were stained with Rat Anti-Mouse TER-119 PE-conjugated Monoclonal Antibody (Catalog # FAB1125P) and either (A) Rat Anti-Mouse CD45 Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # FAB114R) or (B) Rat IgG2B Alexa Fluor 647 Isotype Control (Catalog # IC013R). View our protocol for Staining Membrane-associated Proteins.