Antibody data
- Antibody Data
- Antigen structure
- References [327]
- Comments [0]
- Validations
- Immunohistochemistry [3]
- Flow cytometry [1]
- Other assay [107]
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- Product number
- 14-0452-85 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD45R (B220) Monoclonal Antibody (RA3-6B2), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen. Applications Reported: The RA3-6B2 antibody has been reported for use in flow cytometric analysis, immunoprecipitation, and immunohistochemical staining of frozen or formalin-fixed paraffin embedded tissue sections. RA3-6B2 has also been reported in functional studies of B cells. (Please use Functional Grade purified RA3-6B2, Product # 16-0452, in functional assays). Applications Tested: The RA3-6B2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. The RA3-6B2 antibody has been tested by immunohistochemistry of formalin-fixed paraffin embedded mouse tissue using low and high pH antigen retrieval and can be used at less than or equal to 5 µg/mL. The RA3-6B2 antibody has been tested by immunohistochemistry of frozen mouse tissue and can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human, Mouse
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- RA3-6B2
- Vial size
- 500 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Increased susceptibility to organic dust exposure-induced inflammatory lung disease with enhanced rheumatoid arthritis-associated autoantigen expression in HLA-DR4 transgenic mice.
Trained immunity induction by the inactivated mucosal vaccine MV130 protects against experimental viral respiratory infections.
Stroke induces disease-specific myeloid cells in the brain parenchyma and pia.
Lenvatinib for effectively treating antiangiogenic drug-resistant nasopharyngeal carcinoma.
Transient expansion and myofibroblast conversion of adipogenic lineage precursors mediate bone marrow repair after radiation.
Low Dose Chronic Angiotensin II Induces Selective Senescence of Kidney Endothelial Cells.
A rapid CRISPR competitive assay for in vitro and in vivo discovery of potential drug targets affecting the hematopoietic system.
High-throughput analysis of lung immune cells in a combined murine model of agriculture dust-triggered airway inflammation with rheumatoid arthritis.
Role of CXCL5 in Regulating Chemotaxis of Innate and Adaptive Leukocytes in Infected Lungs Upon Pulmonary Influenza Infection.
Polyvinyl alcohol hydrolysis rate and molecular weight influence human and murine HSC activity ex vivo.
Type 2 diabetic mice enter a state of spontaneous hibernation-like suspended animation following accumulation of uric acid.
Metabolic preconditioning in CD4+ T cells restores inducible immune tolerance in lupus-prone mice.
Targeting Ovarian Carcinoma with TSP-1:CD47 Antagonist TAX2 Activates Anti-Tumor Immunity.
Mechanism of a COVID-19 nanoparticle vaccine candidate that elicits a broadly neutralizing antibody response to SARS-CoV-2 variants.
Impaired HA-specific T follicular helper cell and antibody responses to influenza vaccination are linked to inflammation in humans.
The Role of IgM Antibodies in T Cell Lymphoma Protection in a Novel Model Resembling Anaplastic Large Cell Lymphoma.
Hypoxia-induced miR-210 modulates the inflammatory response and fibrosis upon acute ischemia.
Monocyte-derived dendritic cells link localized secretory IgA deficiency to adaptive immune activation in COPD.
Early innate and adaptive immune perturbations determine long-term severity of chronic virus and Mycobacterium tuberculosis coinfection.
The folate cycle enzyme MTHFD2 induces cancer immune evasion through PD-L1 up-regulation.
Murine myeloid cell MCPIP1 suppresses autoimmunity by regulating B-cell expansion and differentiation.
Ibrutinib does not prevent kidney fibrosis following acute and chronic injury.
Defining the Mechanistic Correlates of Protection Conferred by Whole-Cell Vaccination against Pseudomonas aeruginosa Acute Murine Pneumonia.
Proteostasis in dendritic cells is controlled by the PERK signaling axis independently of ATF4.
Histone deacetylase 3 represses cholesterol efflux during CD4(+) T-cell activation.
Carvacrol ameliorates acute campylobacteriosis in a clinical murine infection model.
Blood-Borne ST6GAL1 Regulates Immunoglobulin Production in B Cells.
Myeloid Cell CK2 Regulates Inflammation and Resistance to Bacterial Infection.
Shp1 Loss Enhances Macrophage Effector Function and Promotes Anti-Tumor Immunity.
Androgen Receptor Signaling Positively Regulates Monocytic Development.
Ischemia Reperfusion Injury Triggers CXCL13 Release and B-Cell Recruitment After Allogenic Kidney Transplantation.
Pituitary Adenylate Cyclase-Activating Polypeptide Alleviates Intestinal, Extra-Intestinal and Systemic Inflammatory Responses during Acute Campylobacter jejuni-induced Enterocolitis in Mice.
The Host-Specific Intestinal Microbiota Composition Impacts Campylobacter coli Infection in a Clinical Mouse Model of Campylobacteriosis.
The lysophospholipase D enzyme Gdpd3 is required to maintain chronic myelogenous leukaemia stem cells.
Aging-Affected MSC Functions and Severity of Periodontal Tissue Destruction in a Ligature-Induced Mouse Periodontitis Model.
Cep55 overexpression promotes genomic instability and tumorigenesis in mice.
Toll-Like Receptor-4 Is Involved in Mediating Intestinal and Extra-Intestinal Inflammation in Campylobacter coli-Infected Secondary Abiotic IL-10(-/-) Mice.
Immune-modulatory Properties of the Octapeptide NAP in Campylobacter jejuni Infected Mice Suffering from Acute Enterocolitis.
Toll-Like Receptor-4 Dependent Intestinal and Systemic Sequelae Following Peroral Campylobacter coli Infection of IL10 Deficient Mice Harboring a Human Gut Microbiota.
Overcoming Immunological Challenges to Helper-Dependent Adenoviral Vector-Mediated Long-Term CFTR Expression in Mouse Airways.
The conundrum of colonization resistance against Campylobacter reloaded: The gut microbota composition in conventional mice does not prevent from Campylobacter coli infection.
Inflammatory osteolysis is regulated by site-specific ISGylation of the scaffold protein NEMO.
Fucoxanthin Ameliorates Atopic Dermatitis Symptoms by Regulating Keratinocytes and Regulatory Innate Lymphoid Cells.
Synergistic factors control kinase-phosphatase organization in B-cells engaged with supported bilayers.
Secreted Phospholipase A(2) Group X Acts as an Adjuvant for Type 2 Inflammation, Leading to an Allergen-Specific Immune Response in the Lung.
Inflammation shapes pathogenesis of murine arrhythmogenic cardiomyopathy.
TIAM2S Mediates Serotonin Homeostasis and Provokes a Pro-Inflammatory Immune Microenvironment Permissive for Colorectal Tumorigenesis.
Microbiota composition and inflammatory immune responses upon peroral application of the commercial competitive exclusion product Aviguard® to microbiota-depleted wildtype mice.
Inflammatory Immune Responses and Gut Microbiota Changes Following Campylobacter coli Infection of IL-10(-/-) Mice with Chronic Colitis.
B cells are sufficient to prime the dominant CD4+ Tfh response to Plasmodium infection.
T-B Lymphocyte Interactions Promote Type 1 Diabetes Independently of SLAM-Associated Protein.
New insights on repeated acoustic injury: Augmentation of cochlear susceptibility and inflammatory reaction resultant of prior acoustic injury.
MYC Instructs and Maintains Pancreatic Adenocarcinoma Phenotype.
Immunopathological properties of the Campylobacter jejuni flagellins and the adhesin CadF as assessed in a clinical murine infection model.
Inducible IL-7 Hyperexpression Influences Lymphocyte Homeostasis and Function and Increases Allograft Rejection.
Tpl2 Protects Against Fulminant Hepatitis Through Mobilization of Myeloid-Derived Suppressor Cells.
Bacteroides fragilis polysaccharide A induces IL-10 secreting B and T cells that prevent viral encephalitis.
Cellular localization and regulation of receptors and enzymes of the endocannabinoid system in intestinal and systemic inflammation.
Inhibition of T cell activation and function by the adaptor protein CIN85.
Aging Induces an Nlrp3 Inflammasome-Dependent Expansion of Adipose B Cells That Impairs Metabolic Homeostasis.
Syndecan 1 is a critical mediator of macropinocytosis in pancreatic cancer.
Bone marrow dendritic cells regulate hematopoietic stem/progenitor cell trafficking.
The Hematopoietic Oxidase NOX2 Regulates Self-Renewal of Leukemic Stem Cells.
Anti-commensal IgG Drives Intestinal Inflammation and Type 17 Immunity in Ulcerative Colitis.
Antigen-presenting ILC3 regulate T cell-dependent IgA responses to colonic mucosal bacteria.
Proapoptotic BIM Impacts B Lymphoid Homeostasis by Limiting the Survival of Mature B Cells in a Cell-Autonomous Manner.
Dysregulated TRAF3 and BCL2 Expression Promotes Multiple Classes of Mature Non-hodgkin B Cell Lymphoma in Mice.
ATF3 Sustains IL-22-Induced STAT3 Phosphorylation to Maintain Mucosal Immunity Through Inhibiting Phosphatases.
Systemic ST6Gal-1 Is a Pro-survival Factor for Murine Transitional B Cells.
Identification of Distinct Unmutated Chronic Lymphocytic Leukemia Subsets in Mice Based on Their T Cell Dependency.
Tumor-immune profiling of murine syngeneic tumor models as a framework to guide mechanistic studies and predict therapy response in distinct tumor microenvironments.
High-affinity IgM(+) memory B cells are defective in differentiation into IgM antibody-secreting cells by re-stimulation with a T cell-dependent antigen.
LaminA/C regulates epigenetic and chromatin architecture changes upon aging of hematopoietic stem cells.
Targeted delivery of immune therapeutics to lymph nodes prolongs cardiac allograft survival.
Microbial symbionts regulate the primary Ig repertoire.
Establishment of regulatory elements during erythro-megakaryopoiesis identifies hematopoietic lineage-commitment points.
Early cellular interactions and immune transcriptome profiles in human factor VIII-exposed hemophilia A mice.
A prominent air pollutant, Indeno[1,2,3-cd]pyrene, enhances allergic lung inflammation via aryl hydrocarbon receptor.
Host Tumor Suppressor p18(INK4c) Functions as a Potent Cell-Intrinsic Inhibitor of Murine Gammaherpesvirus 68 Reactivation and Pathogenesis.
Adrenergic nerve degeneration in bone marrow drives aging of the hematopoietic stem cell niche.
Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis.
Anti-inflammatory Effects of the Octapeptide NAP in Human Microbiota-Associated Mice Suffering from Subacute Ileitis.
miR-143/145 differentially regulate hematopoietic stem and progenitor activity through suppression of canonical TGFβ signaling.
Group 2 Innate Lymphoid Cells Attenuate Inflammatory Arthritis and Protect from Bone Destruction in Mice.
A non-cell-autonomous role for Pml in the maintenance of leukemia from the niche.
Targeting murine leukemic stem cells by antibody functionalized mesoporous silica nanoparticles.
Oxysterol Sensing through the Receptor GPR183 Promotes the Lymphoid-Tissue-Inducing Function of Innate Lymphoid Cells and Colonic Inflammation.
Tanshinone IIA and Astragaloside IV promote the angiogenesis of mesenchymal stem cell-derived endothelial cell-like cells via upregulation of Cx37, Cx40 and Cx43.
Multiple Levels of Control Determine How E4bp4/Nfil3 Regulates NK Cell Development.
Immunomodulatory role of Keratin 76 in oral and gastric cancer.
Inactivation of mTORC1 Signaling in Osterix-Expressing Cells Impairs B-cell Differentiation.
Multidrug-resistant Pseudomonas aeruginosa aggravates inflammatory responses in murine chronic colitis.
Absence of the Non-Signalling Chemerin Receptor CCRL2 Exacerbates Acute Inflammatory Responses In Vivo.
Blocking CTLA-4 while priming with a whole cell vaccine reshapes the oligoclonal T cell infiltrate and eradicates tumors in an orthotopic glioma model.
The Interleukin (IL)-1R1 pathway is a critical negative regulator of PyMT-mediated mammary tumorigenesis and pulmonary metastasis.
Early derivation of IgM memory cells and bone marrow plasmablasts.
Autophagy is a signature of a signaling network that maintains hematopoietic stem cells.
Multiple functional therapeutic effects of TnP: A small stable synthetic peptide derived from fish venom in a mouse model of multiple sclerosis.
Human and Mouse Hematopoietic Stem Cells Are a Depot for Dormant Mycobacterium tuberculosis.
Early IL-6 signalling promotes IL-27 dependent maturation of regulatory T cells in the lungs and resolution of viral immunopathology.
Polymicrobial sepsis impairs bystander recruitment of effector cells to infected skin despite optimal sensing and alarming function of skin resident memory CD8 T cells.
DNA-binding of the Tet-transactivator curtails antigen-induced lymphocyte activation in mice.
The microanatomic segregation of selection by apoptosis in the germinal center.
EZH2 enables germinal centre formation through epigenetic silencing of CDKN1A and an Rb-E2F1 feedback loop.
Treatment with Allogenic Mesenchymal Stromal Cells in a Murine Model of Systemic Lupus Erythematosus.
Oncostatin M drives intestinal inflammation and predicts response to tumor necrosis factor-neutralizing therapy in patients with inflammatory bowel disease.
Differential cytokine contributions of perivascular haematopoietic stem cell niches.
Bispecific antibody targets multiple Pseudomonas aeruginosa evasion mechanisms in the lung vasculature.
Functional characterization of the lysosomal membrane protein TMEM192 in mice.
LRCH1 interferes with DOCK8-Cdc42-induced T cell migration and ameliorates experimental autoimmune encephalomyelitis.
Th2 responses are primed by skin dendritic cells with distinct transcriptional profiles.
Astaxanthin attenuates total body irradiation-induced hematopoietic system injury in mice via inhibition of oxidative stress and apoptosis.
The podoplanin-CLEC-2 axis inhibits inflammation in sepsis.
Alternative activation generates IL-10 producing type 2 innate lymphoid cells.
Differential Role of B Cells and IL-17 Versus IFN-γ During Early and Late Rejection of Pig Islet Xenografts in Mice.
Irgm1 coordinately regulates autoimmunity and host defense at select mucosal surfaces.
Epithelial Fli1 deficiency drives systemic autoimmunity and fibrosis: Possible roles in scleroderma.
Combined antiangiogenic and anti-PD-L1 therapy stimulates tumor immunity through HEV formation.
Orally administered β-glucan attenuates the Th2 response in a model of airway hypersensitivity.
PD-1 pathway-mediated regulation of islet-specific CD4(+) T cell subsets in autoimmune diabetes.
CRISPR-Mediated Triple Knockout of SLAMF1, SLAMF5 and SLAMF6 Supports Positive Signaling Roles in NKT Cell Development.
Proliferation and Recruitment Contribute to Myocardial Macrophage Expansion in Chronic Heart Failure.
Reprogramming the Local Lymph Node Microenvironment Promotes Tolerance that Is Systemic and Antigen Specific.
LYN- and AIRE-mediated tolerance checkpoint defects synergize to trigger organ-specific autoimmunity.
M-CSF improves protection against bacterial and fungal infections after hematopoietic stem/progenitor cell transplantation.
Blood Stem Cell Activity Is Arrested by Th1-Mediated Injury Preventing Engraftment following Nonmyeloablative Conditioning.
Deficiency of base excision repair enzyme NEIL3 drives increased predisposition to autoimmunity.
Balancing intestinal and systemic inflammation through cell type-specific expression of the aryl hydrocarbon receptor repressor.
B-cell survival and development controlled by the coordination of NF-κB family members RelB and cRel.
Chronic Gastrointestinal Nematode Infection Mutes Immune Responses to Mycobacterial Infection Distal to the Gut.
TNF and CD28 Signaling Play Unique but Complementary Roles in the Systemic Recruitment of Innate Immune Cells after Staphylococcus aureus Enterotoxin A Inhalation.
Bmp signaling in colonic mesenchyme regulates stromal microenvironment and protects from polyposis initiation.
Group 2 innate lymphoid cells utilize the IRF4-IL-9 module to coordinate epithelial cell maintenance of lung homeostasis.
The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells.
A20 Deficiency in Lung Epithelial Cells Protects against Influenza A Virus Infection.
Postnatal lethality and abnormal development of foregut and spleen in Ndrg4 mutant mice.
miR-150 regulates obesity-associated insulin resistance by controlling B cell functions.
This paper is the winner of an SFB Award in the Hospital Intern, Residency category: Peptide biomaterials raising adaptive immune responses in wound healing contexts.
Murine iPSC-Derived Macrophages as a Tool for Disease Modeling of Hereditary Pulmonary Alveolar Proteinosis due to Csf2rb Deficiency.
Resident T Cells Are Unable To Control Herpes Simplex Virus-1 Activity in the Brain Ependymal Region during Latency.
Trypanosoma cruzi Causes Paralyzing Systemic Necrotizing Vasculitis Driven by Pathogen-Specific Type I Immunity in Mice.
Protective Role for B-1b B Cells and IgM in Obesity-Associated Inflammation, Glucose Intolerance, and Insulin Resistance.
NFκB1 is essential to prevent the development of multiorgan autoimmunity by limiting IL-6 production in follicular B cells.
Type I IFN Does Not Promote Susceptibility to Foodborne Listeria monocytogenes.
LNK/SH2B3 regulates IL-7 receptor signaling in normal and malignant B-progenitors.
Vitamin A Impairs the Reprogramming of Tregs into IL-17-Producing Cells during Intestinal Inflammation.
The immunosenescence-related gene Zizimin2 is associated with early bone marrow B cell development and marginal zone B cell formation.
Evaluation of the BDCA2-DTR Transgenic Mouse Model in Chronic and Acute Inflammation.
Mitochondrial ATP transporter Ant2 depletion impairs erythropoiesis and B lymphopoiesis.
Inflammation rapidly reorganizes mouse bone marrow B cells and their environment in conjunction with early IgM responses.
Metformin ameliorates ionizing irradiation-induced long-term hematopoietic stem cell injury in mice.
Endothelial Gata5 transcription factor regulates blood pressure.
mTORC1 and mTORC2 selectively regulate CD8⁺ T cell differentiation.
Limited miR-17-92 overexpression drives hematologic malignancies.
Enhanced CD8 T cell responses through GITR-mediated costimulation resolve chronic viral infection.
LKB1 inhibition of NF-κB in B cells prevents T follicular helper cell differentiation and germinal center formation.
Peptide-MHC-I from Endogenous Antigen Outnumber Those from Exogenous Antigen, Irrespective of APC Phenotype or Activation.
Disruption of p21-activated kinase 1 gene diminishes atherosclerosis in apolipoprotein E-deficient mice.
SOCS3 Deficiency in Myeloid Cells Promotes Tumor Development: Involvement of STAT3 Activation and Myeloid-Derived Suppressor Cells.
Control of signaling-mediated clearance of apoptotic cells by the tumor suppressor p53.
Structural and functional features of central nervous system lymphatic vessels.
Comparison of tamoxifen and letrozole response in mammary preneoplasia of ER and aromatase overexpressing mice defines an immune-associated gene signature linked to tamoxifen resistance.
The CLEC-2-podoplanin axis controls the contractility of fibroblastic reticular cells and lymph node microarchitecture.
CCR7-dependent trafficking of RORγ⁺ ILCs creates a unique microenvironment within mucosal draining lymph nodes.
F-box protein FBXW7 inhibits cancer metastasis in a non-cell-autonomous manner.
Role of TP53 mutations in the origin and evolution of therapy-related acute myeloid leukaemia.
Small-molecule inhibitors targeting INK4 protein p18(INK4C) enhance ex vivo expansion of haematopoietic stem cells.
The E-Id protein axis modulates the activities of the PI3K-AKT-mTORC1-Hif1a and c-myc/p19Arf pathways to suppress innate variant TFH cell development, thymocyte expansion, and lymphomagenesis.
ELF5 Drives Lung Metastasis in Luminal Breast Cancer through Recruitment of Gr1+ CD11b+ Myeloid-Derived Suppressor Cells.
Aging-associated inflammation promotes selection for adaptive oncogenic events in B cell progenitors.
MHC-mismatched mixed chimerism augments thymic regulatory T-cell production and prevents relapse of EAE in mice.
Depletion of B220(+)NK1.1(+) cells enhances the rejection of established melanoma by tumor-specific CD4(+) T cells.
TNF signals are dispensable for the generation of CD23+ CD21/35-high CD1d-high B cells in inflamed lymph nodes.
Deficient Natural Killer Dendritic Cell Responses Underlay the Induction of Theiler's Virus-Induced Autoimmunity.
Kindlin-3-mediated integrin adhesion is dispensable for quiescent but essential for activated hematopoietic stem cells.
Serotonin and insulin-like peptides modulate leucokinin-producing neurons that affect feeding and water homeostasis in Drosophila.
Splenic red pulp macrophages are intrinsically superparamagnetic and contaminate magnetic cell isolates.
Setd1a regulates progenitor B-cell-to-precursor B-cell development through histone H3 lysine 4 trimethylation and Ig heavy-chain rearrangement.
Identification of Toll-like receptor 9 as parapoxvirus ovis-sensing receptor in plasmacytoid dendritic cells.
Nfatc2 and Tob1 have non-overlapping function in T cell negative regulation and tumorigenesis.
Purinergic signaling on leukocytes infiltrating the LPS-injured lung.
Activated macrophage survival is coordinated by TAK1 binding proteins.
B-cell-intrinsic hepatitis C virus expression leads to B-cell-lymphomagenesis and induction of NF-κB signalling.
Administration of the resveratrol analogues isorhapontigenin and heyneanol-A protects mice hematopoietic cells against irradiation injuries.
Antigen signal strength during priming determines effector CD4 T cell function and antigen sensitivity during influenza virus challenge.
Thymic epithelium determines a spontaneous chronic neuritis in Icam1(tm1Jcgr)NOD mice.
A NOTCH1-driven MYC enhancer promotes T cell development, transformation and acute lymphoblastic leukemia.
CD28 expression is required after T cell priming for helper T cell responses and protective immunity to infection.
Progressive replacement of embryo-derived cardiac macrophages with age.
Cutting edge: CD8+ recent thymic emigrants exhibit increased responses to low-affinity ligands and improved access to peripheral sites of inflammation.
Internalization and TLR-dependent type I interferon production by monocytes in response to Toxoplasma gondii.
INAM plays a critical role in IFN-γ production by NK cells interacting with polyinosinic-polycytidylic acid-stimulated accessory cells.
Lasting antibody responses are mediated by a combination of newly formed and established bone marrow plasma cells drawn from clonally distinct precursors.
Transcription elongation factor ELL2 drives Ig secretory-specific mRNA production and the unfolded protein response.
Costimulation via the tumor-necrosis factor receptor superfamily couples TCR signal strength to the thymic differentiation of regulatory T cells.
MyD88-dependent immunity to a natural model of vaccinia virus infection does not involve Toll-like receptor 2.
Memory B cells form in aged mice despite impaired affinity maturation and germinal center kinetics.
Combined MEK and JAK inhibition abrogates murine myeloproliferative neoplasm.
Intrinsic TGF-β signaling promotes age-dependent CD8+ T cell polyfunctionality attrition.
12-Hydroxyheptadecatrienoic acid promotes epidermal wound healing by accelerating keratinocyte migration via the BLT2 receptor.
Ly49 family receptors are required for cancer immunosurveillance mediated by natural killer cells.
Paradoxical resistance to high-fat diet-induced obesity and altered macrophage polarization in mineralocorticoid receptor-overexpressing mice.
Lipoxin A₄ modulates adaptive immunity by decreasing memory B-cell responses via an ALX/FPR2-dependent mechanism.
B cell-specific loss of Lyn kinase leads to autoimmunity.
Central memory CD4+ T cells are responsible for the recombinant Bacillus Calmette-Guérin ΔureC::hly vaccine's superior protection against tuberculosis.
Rhof promotes murine marginal zone B cell development.
APRIL mediates peritoneal B-1 cell homeostasis.
In utero depletion of fetal hematopoietic stem cells improves engraftment after neonatal transplantation in mice.
Recognition of tumor cells by Dectin-1 orchestrates innate immune cells for anti-tumor responses.
The transcription factor E4bp4/Nfil3 controls commitment to the NK lineage and directly regulates Eomes and Id2 expression.
IκBε is a key regulator of B cell expansion by providing negative feedback on cRel and RelA in a stimulus-specific manner.
TDP2-dependent non-homologous end-joining protects against topoisomerase II-induced DNA breaks and genome instability in cells and in vivo.
A New Murine Model for Gastrointestinal Anthrax Infection.
In vivo knockdown of TAK1 accelerates bone marrow proliferation/differentiation and induces systemic inflammation.
Transient B-cell depletion combined with apoptotic donor splenocytes induces xeno-specific T- and B-cell tolerance to islet xenografts.
COP1 targets C/EBPα for degradation and induces acute myeloid leukemia via Trib1.
Macrophage microRNA-155 promotes cardiac hypertrophy and failure.
A component of the mir-17-92 polycistronic oncomir promotes oncogene-dependent apoptosis.
Neuronal and nonneuronal cholinergic structures in the mouse gastrointestinal tract and spleen.
A canonical to non-canonical Wnt signalling switch in haematopoietic stem-cell ageing.
MIP-1α/CCL3-mediated maintenance of leukemia-initiating cells in the initiation process of chronic myeloid leukemia.
Natural IgG antibodies provide innate protection against ficolin-opsonized bacteria.
Ebf1 and c-Myb repress rag transcription downstream of Stat5 during early B cell development.
Natural killer cells are required for extramedullary hematopoiesis following murine cytomegalovirus infection.
Critical role of SAP in progression and reactivation but not maintenance of T cell-dependent humoral immunity.
SAP modulates B cell functions in a genetic background-dependent manner.
Histone demethylase Lsd1 represses hematopoietic stem and progenitor cell signatures during blood cell maturation.
RIP1-driven autoinflammation targets IL-1α independently of inflammasomes and RIP3.
Reciprocal effects of rab7 deletion in activated and neglected T cells.
MYBL2 is a sub-haploinsufficient tumor suppressor gene in myeloid malignancy.
Avirulent Toxoplasma gondii generates therapeutic antitumor immunity by reversing immunosuppression in the ovarian cancer microenvironment.
Adhesion to substrates induces dendritic cell endothelization and decreases immunological response.
Shared and distinct transcriptional programs underlie the hybrid nature of iNKT cells.
TiF1-gamma plays an essential role in murine hematopoiesis and regulates transcriptional elongation of erythroid genes.
The intramembrane protease SPPL2a promotes B cell development and controls endosomal traffic by cleavage of the invariant chain.
Cbx4 regulates the proliferation of thymic epithelial cells and thymus function.
T cell-positive selection uses self-ligand binding strength to optimize repertoire recognition of foreign antigens.
HDL and Glut1 inhibition reverse a hypermetabolic state in mouse models of myeloproliferative disorders.
The kinase mTOR modulates the antibody response to provide cross-protective immunity to lethal infection with influenza virus.
An intact immune system is required for the anticancer activities of histone deacetylase inhibitors.
Temporal requirements for B cells in the establishment of CD4 T cell memory.
Hematopoietic progenitor cell lines with myeloid and lymphoid potential.
The β2 integrin-kindlin-3 interaction is essential for T-cell homing but dispensable for T-cell activation in vivo.
Myeloid-derived suppressor cells play crucial roles in the regulation of mouse collagen-induced arthritis.
Inflammatory neovascularization during graft-versus-host disease is regulated by αv integrin and miR-100.
T cell factor 1 is required for group 2 innate lymphoid cell generation.
Extra-thymic physiological T lineage progenitor activity is exclusively confined to cells expressing either CD127, CD90, or high levels of CD117.
Dominant Role of CD80-CD86 Over CD40 and ICOSL in the Massive Polyclonal B Cell Activation Mediated by LAT(Y136F) CD4(+) T Cells.
Incomplete TCR-β allelic exclusion accelerates spontaneous autoimmune arthritis in K/BxN TCR transgenic mice.
Lymphotoxin-beta receptor blockade induces inflammation and fibrosis in tolerized cardiac allografts.
NK cells are required for costimulatory blockade induced tolerance to vascularized allografts.
Activation-induced cytidine deaminase-initiated off-target DNA breaks are detected and resolved during S phase.
PIDDosome-independent tumor suppression by Caspase-2.
Molecular definition of the identity and activation of natural killer cells.
Kruppel-like factor 7 overexpression suppresses hematopoietic stem and progenitor cell function.
Peroxisome proliferator-activated receptor γ B cell-specific-deficient mice have an impaired antibody response.
A role for GPx3 in activity of normal and leukemia stem cells.
Dll4-Notch signaling in Flt3-independent dendritic cell development and autoimmunity in mice.
CD70-driven costimulation induces survival or Fas-mediated apoptosis of T cells depending on antigenic load.
Exposure to nicotine adversely affects the dendritic cell system and compromises host response to vaccination.
Zbtb46 expression distinguishes classical dendritic cells and their committed progenitors from other immune lineages.
Fibroblast growth factor-7 partially reverses murine thymocyte progenitor aging by repression of Ink4a.
Lung B cells promote early pathogen dissemination and hasten death from inhalation anthrax.
Identification of a genetic locus controlling bacteria-driven colitis and associated cancer through effects on innate inflammation.
IL-21 promotes lupus-like disease in chronic graft-versus-host disease through both CD4 T cell- and B cell-intrinsic mechanisms.
Role of mast cells and basophils in IgE responses and in allergic airway hyperresponsiveness.
Critical role of B cell lymphoma 10 in BAFF-regulated NF-κB activation and survival of anergic B cells.
MicroRNA profiling identifies microRNA-155 as an adverse mediator of cardiac injury and dysfunction during acute viral myocarditis.
PAD4-mediated neutrophil extracellular trap formation is not required for immunity against influenza infection.
FTY720 reduces post-ischemic brain lymphocyte influx but does not improve outcome in permanent murine cerebral ischemia.
Genetics and the environment converge to dysregulate N-glycosylation in multiple sclerosis.
The inflammasome adaptor ASC regulates the function of adaptive immune cells by controlling Dock2-mediated Rac activation and actin polymerization.
NKT cells are required for complete Freund's adjuvant-mediated protection from autoimmune diabetes.
Anti-CD180 (RP105) activates B cells to rapidly produce polyclonal Ig via a T cell and MyD88-independent pathway.
Chemokines control naive CD8+ T cell selection of optimal lymph node antigen presenting cells.
Identification of semaphorin 4B as a negative regulator of basophil-mediated immune responses.
L2pB1: a new player in autoimmunity.
Recent thymic emigrants are biased against the T-helper type 1 and toward the T-helper type 2 effector lineage.
RNA-seq analysis of 2 closely related leukemia clones that differ in their self-renewal capacity.
Nuclear export of the NF-κB inhibitor IκBα is required for proper B cell and secondary lymphoid tissue formation.
A subset of interleukin-21+ chemokine receptor CCR9+ T helper cells target accessory organs of the digestive system in autoimmunity.
T-bet and eomesodermin are required for T cell-mediated antitumor immune responses.
Glycosidic Tn-based vaccines targeting dermal dendritic cells favor germinal center B-cell development and potent antibody response in the absence of adjuvant.
Store-operated Ca2+ entry through ORAI1 is critical for T cell-mediated autoimmunity and allograft rejection.
Notch dimerization is required for leukemogenesis and T-cell development.
Steroid-resistant lymphatic remodeling in chronically inflamed mouse airways.
Dendritic cell (DC)-specific targeting reveals Stat3 as a negative regulator of DC function.
Separation of graft-versus-host disease from graft-versus-leukemia responses by targeting CC-chemokine receptor 7 on donor T cells.
Mad3 negatively regulates B cell differentiation in the spleen by inducing Id2 expression.
Intermediate-term hematopoietic stem cells with extended but time-limited reconstitution potential.
AUF1 is involved in splenic follicular B cell maintenance.
Suppression of B-cell lymphomagenesis by the BH3-only proteins Bmf and Bad.
The PI3K isoforms p110alpha and p110delta are essential for pre-B cell receptor signaling and B cell development.
A novel heat-shock protein coinducer boosts stress protein Hsp70 to activate T cell regulation of inflammation in autoimmune arthritis.
Toll-like receptor 3-mediated suppression of TRAMP prostate cancer shows the critical role of type I interferons in tumor immune surveillance.
Gene expression profiling of leukemia stem cells.
PI3 kinase signals BCR-dependent mature B cell survival.
Id1 represses osteoclast-dependent transcription and affects bone formation and hematopoiesis.
Chemokine-like receptor-1 expression by central nervous system-infiltrating leukocytes and involvement in a model of autoimmune demyelinating disease.
Chronic CD70-driven costimulation impairs IgG responses by instructing T cells to inhibit germinal center B cell formation through FasL-Fas interactions.
A critical role of TAK1 in B-cell receptor-mediated nuclear factor kappaB activation.
Dynamic imaging of T cell-parasite interactions in the brains of mice chronically infected with Toxoplasma gondii.
Critical roles of lysosomal acid lipase in T cell development and function.
Oncogenic Kras initiates leukemia in hematopoietic stem cells.
CD93 is required for maintenance of antibody secretion and persistence of plasma cells in the bone marrow niche.
AID constrains germinal center size by rendering B cells susceptible to apoptosis.
Apoptotic cells promote their own clearance and immune tolerance through activation of the nuclear receptor LXR.
Migratory and antigen presentation functions of IFN-producing killer dendritic cells.
Peripheral dendritic cells are essential for both the innate and adaptive antiviral immune responses in the central nervous system.
Fundamental differences in the dynamics of CNS lesion development and composition in MP4- and MOG peptide 35-55-induced experimental autoimmune encephalomyelitis.
Age-dependent tolerance to an endogenous tumor-associated antigen.
The receptor tyrosine kinase Flt3 is required for dendritic cell development in peripheral lymphoid tissues.
Donor major histocompatibility complex class I expression determines the outcome of prenatal transplantation.
Regulatory role of B cells in a murine model of allergic airway disease.
Regulatory role of B cells in a murine model of allergic airway disease.
Activation-induced cytidine deaminase deficiency causes organ-specific autoimmune disease.
p27 deficiency cooperates with Bcl-2 but not Bax to promote T-cell lymphoma.
Phospholipase Cgamma2 contributes to light-chain gene activation and receptor editing.
N-glycan processing deficiency promotes spontaneous inflammatory demyelination and neurodegeneration.
Selective depletion of Foxp3+ regulatory T cells induces a scurfy-like disease.
Murine CXCL14 is dispensable for dendritic cell function and localization within peripheral tissues.
Small intestine lamina propria dendritic cells promote de novo generation of Foxp3 T reg cells via retinoic acid.
Identification of a radio-resistant and cycling dermal dendritic cell population in mice and men.
Essential role of phospholipase C gamma 2 in early B-cell development and Myc-mediated lymphomagenesis.
A role for brain-derived neurotrophic factor in B cell development.
Impaired immune responses and B-cell proliferation in mice lacking the Id3 gene.
Identification of monoclonal antibodies for immunohistochemical staining of feline B lymphocytes in frozen and formalin-fixed paraffin-embedded tissues.
Poole JA, Mikuls TR, Thiele GM, Gaurav R, Nelson AJ, Duryee MJ, Mitra A, Hunter C, Wyatt TA, England BR, Ascherman DP
Respiratory research 2022 Jun 18;23(1):160
Respiratory research 2022 Jun 18;23(1):160
Trained immunity induction by the inactivated mucosal vaccine MV130 protects against experimental viral respiratory infections.
Brandi P, Conejero L, Cueto FJ, Martínez-Cano S, Dunphy G, Gómez MJ, Relaño C, Saz-Leal P, Enamorado M, Quintas A, Dopazo A, Amores-Iniesta J, Del Fresno C, Nistal-Villán E, Ardavín C, Nieto A, Casanovas M, Subiza JL, Sancho D
Cell reports 2022 Jan 4;38(1):110184
Cell reports 2022 Jan 4;38(1):110184
Stroke induces disease-specific myeloid cells in the brain parenchyma and pia.
Beuker C, Schafflick D, Strecker JK, Heming M, Li X, Wolbert J, Schmidt-Pogoda A, Thomas C, Kuhlmann T, Aranda-Pardos I, A-Gonzalez N, Kumar PA, Werner Y, Kilic E, Hermann DM, Wiendl H, Stumm R, Meyer Zu Hörste G, Minnerup J
Nature communications 2022 Feb 17;13(1):945
Nature communications 2022 Feb 17;13(1):945
Lenvatinib for effectively treating antiangiogenic drug-resistant nasopharyngeal carcinoma.
Sun Q, Wang Y, Ji H, Sun X, Xie S, Chen L, Li S, Zeng W, Chen R, Tang Q, Zuo J, Hou L, Hosaka K, Lu Y, Liu Y, Ye Y, Yang Y
Cell death & disease 2022 Aug 19;13(8):724
Cell death & disease 2022 Aug 19;13(8):724
Transient expansion and myofibroblast conversion of adipogenic lineage precursors mediate bone marrow repair after radiation.
Zhong L, Yao L, Holdreith N, Yu W, Gui T, Miao Z, Elkaim Y, Li M, Gong Y, Pacifici M, Maity A, Busch TM, Joeng KS, Cengel K, Seale P, Tong W, Qin L
JCI insight 2022 Apr 8;7(7)
JCI insight 2022 Apr 8;7(7)
Low Dose Chronic Angiotensin II Induces Selective Senescence of Kidney Endothelial Cells.
Khan I, Schmidt MO, Kallakury B, Jain S, Mehdikhani S, Levi M, Mendonca M, Welch W, Riegel AT, Wilcox CS, Wellstein A
Frontiers in cell and developmental biology 2021;9:782841
Frontiers in cell and developmental biology 2021;9:782841
A rapid CRISPR competitive assay for in vitro and in vivo discovery of potential drug targets affecting the hematopoietic system.
Shen Y, Jiang L, Iyer VS, Raposo B, Dubnovitsky A, Boddul SV, Kasza Z, Wermeling F
Computational and structural biotechnology journal 2021;19:5360-5370
Computational and structural biotechnology journal 2021;19:5360-5370
High-throughput analysis of lung immune cells in a combined murine model of agriculture dust-triggered airway inflammation with rheumatoid arthritis.
Gaurav R, Mikuls TR, Thiele GM, Nelson AJ, Niu M, Guda C, Eudy JD, Barry AE, Wyatt TA, Romberger DJ, Duryee MJ, England BR, Poole JA
PloS one 2021;16(2):e0240707
PloS one 2021;16(2):e0240707
Role of CXCL5 in Regulating Chemotaxis of Innate and Adaptive Leukocytes in Infected Lungs Upon Pulmonary Influenza Infection.
Guo L, Li N, Yang Z, Li H, Zheng H, Yang J, Chen Y, Zhao X, Mei J, Shi H, Worthen GS, Liu L
Frontiers in immunology 2021;12:785457
Frontiers in immunology 2021;12:785457
Polyvinyl alcohol hydrolysis rate and molecular weight influence human and murine HSC activity ex vivo.
Sudo K, Yamazaki S, Wilkinson AC, Nakauchi H, Nakamura Y
Stem cell research 2021 Oct;56:102531
Stem cell research 2021 Oct;56:102531
Type 2 diabetic mice enter a state of spontaneous hibernation-like suspended animation following accumulation of uric acid.
Zhao Y, Cheng R, Zhao Y, Ge W, Yang Y, Ding Z, Xu X, Wang Z, Wu Z, Zhang J
The Journal of biological chemistry 2021 Oct;297(4):101166
The Journal of biological chemistry 2021 Oct;297(4):101166
Metabolic preconditioning in CD4+ T cells restores inducible immune tolerance in lupus-prone mice.
Wilson CS, Stocks BT, Hoopes EM, Rhoads JP, McNew KL, Major AS, Moore DJ
JCI insight 2021 Oct 8;6(19)
JCI insight 2021 Oct 8;6(19)
Targeting Ovarian Carcinoma with TSP-1:CD47 Antagonist TAX2 Activates Anti-Tumor Immunity.
Jeanne A, Sarazin T, Charlé M, Moali C, Fichel C, Boulagnon-Rombi C, Callewaert M, Andry MC, Diesis E, Delolme F, Rioult D, Dedieu S
Cancers 2021 Oct 7;13(19)
Cancers 2021 Oct 7;13(19)
Mechanism of a COVID-19 nanoparticle vaccine candidate that elicits a broadly neutralizing antibody response to SARS-CoV-2 variants.
Zhang YN, Paynter J, Sou C, Fourfouris T, Wang Y, Abraham C, Ngo T, Zhang Y, He L, Zhu J
Science advances 2021 Oct 22;7(43):eabj3107
Science advances 2021 Oct 22;7(43):eabj3107
Impaired HA-specific T follicular helper cell and antibody responses to influenza vaccination are linked to inflammation in humans.
Hill DL, Whyte CE, Innocentin S, Lee JL, Dooley J, Wang J, James EA, Lee JC, Kwok WW, Zand MS, Liston A, Carr EJ, Linterman MA
eLife 2021 Nov 2;10
eLife 2021 Nov 2;10
The Role of IgM Antibodies in T Cell Lymphoma Protection in a Novel Model Resembling Anaplastic Large Cell Lymphoma.
Jiang C, Zhao ML, Ramos L, Dobaczewska K, Herbert R, Hobbie K, Mikulski Z, Verkoczy L, Diaz M
Journal of immunology (Baltimore, Md. : 1950) 2021 May 15;206(10):2468-2477
Journal of immunology (Baltimore, Md. : 1950) 2021 May 15;206(10):2468-2477
Hypoxia-induced miR-210 modulates the inflammatory response and fibrosis upon acute ischemia.
Zaccagnini G, Greco S, Longo M, Maimone B, Voellenkle C, Fuschi P, Carrara M, Creo P, Maselli D, Tirone M, Mazzone M, Gaetano C, Spinetti G, Martelli F
Cell death & disease 2021 May 1;12(5):435
Cell death & disease 2021 May 1;12(5):435
Monocyte-derived dendritic cells link localized secretory IgA deficiency to adaptive immune activation in COPD.
Richmond BW, Mansouri S, Serezani A, Novitskiy S, Blackburn JB, Du RH, Fuseini H, Gutor S, Han W, Schaff J, Vasiukov G, Xin MK, Newcomb DC, Jin L, Blackwell TS, Polosukhin VV
Mucosal immunology 2021 Mar;14(2):431-442
Mucosal immunology 2021 Mar;14(2):431-442
Early innate and adaptive immune perturbations determine long-term severity of chronic virus and Mycobacterium tuberculosis coinfection.
Xu W, Snell LM, Guo M, Boukhaled G, Macleod BL, Li M, Tullius MV, Guidos CJ, Tsao MS, Divangahi M, Horwitz MA, Liu J, Brooks DG
Immunity 2021 Mar 9;54(3):526-541.e7
Immunity 2021 Mar 9;54(3):526-541.e7
The folate cycle enzyme MTHFD2 induces cancer immune evasion through PD-L1 up-regulation.
Shang M, Yang H, Yang R, Chen T, Fu Y, Li Y, Fang X, Zhang K, Zhang J, Li H, Cao X, Gu J, Xiao J, Zhang Q, Liu X, Yu Q, Wang T
Nature communications 2021 Mar 29;12(1):1940
Nature communications 2021 Mar 29;12(1):1940
Murine myeloid cell MCPIP1 suppresses autoimmunity by regulating B-cell expansion and differentiation.
Dobosz E, Lorenz G, Ribeiro A, Würf V, Wadowska M, Kotlinowski J, Schmaderer C, Potempa J, Fu M, Koziel J, Lech M
Disease models & mechanisms 2021 Mar 18;14(3)
Disease models & mechanisms 2021 Mar 18;14(3)
Ibrutinib does not prevent kidney fibrosis following acute and chronic injury.
Belliere J, Casemayou A, Colliou E, El Hachem H, Kounde C, Piedrafita A, Feuillet G, Schanstra JP, Faguer S
Scientific reports 2021 Jun 7;11(1):11985
Scientific reports 2021 Jun 7;11(1):11985
Defining the Mechanistic Correlates of Protection Conferred by Whole-Cell Vaccination against Pseudomonas aeruginosa Acute Murine Pneumonia.
Sen-Kilic E, Blackwood CB, Huckaby AB, Horspool AM, Weaver KL, Malkowski AC, Witt WT, Bevere JR, Damron FH, Barbier M
Infection and immunity 2021 Jan 19;89(2)
Infection and immunity 2021 Jan 19;89(2)
Proteostasis in dendritic cells is controlled by the PERK signaling axis independently of ATF4.
Mendes A, Gigan JP, Rodriguez Rodrigues C, Choteau SA, Sanseau D, Barros D, Almeida C, Camosseto V, Chasson L, Paton AW, Paton JC, Argüello RJ, Lennon-Duménil AM, Gatti E, Pierre P
Life science alliance 2021 Feb;4(2)
Life science alliance 2021 Feb;4(2)
Histone deacetylase 3 represses cholesterol efflux during CD4(+) T-cell activation.
Wilfahrt D, Philips RL, Lama J, Kizerwetter M, Shapiro MJ, McCue SA, Kennedy MM, Rajcula MJ, Zeng H, Shapiro VS
eLife 2021 Dec 2;10
eLife 2021 Dec 2;10
Carvacrol ameliorates acute campylobacteriosis in a clinical murine infection model.
Mousavi S, Schmidt AM, Escher U, Kittler S, Kehrenberg C, Thunhorst E, Bereswill S, Heimesaat MM
Gut pathogens 2020;12:2
Gut pathogens 2020;12:2
Blood-Borne ST6GAL1 Regulates Immunoglobulin Production in B Cells.
Irons EE, Punch PR, Lau JTY
Frontiers in immunology 2020;11:617
Frontiers in immunology 2020;11:617
Myeloid Cell CK2 Regulates Inflammation and Resistance to Bacterial Infection.
Larson SR, Bortell N, Illies A, Crisler WJ, Matsuda JL, Lenz LL
Frontiers in immunology 2020;11:590266
Frontiers in immunology 2020;11:590266
Shp1 Loss Enhances Macrophage Effector Function and Promotes Anti-Tumor Immunity.
Myers DR, Abram CL, Wildes D, Belwafa A, Welsh AMN, Schulze CJ, Choy TJ, Nguyen T, Omaque N, Hu Y, Singh M, Hansen R, Goldsmith MA, Quintana E, Smith JAM, Lowell CA
Frontiers in immunology 2020;11:576310
Frontiers in immunology 2020;11:576310
Androgen Receptor Signaling Positively Regulates Monocytic Development.
Consiglio CR, Gollnick SO
Frontiers in immunology 2020;11:519383
Frontiers in immunology 2020;11:519383
Ischemia Reperfusion Injury Triggers CXCL13 Release and B-Cell Recruitment After Allogenic Kidney Transplantation.
Kreimann K, Jang MS, Rong S, Greite R, von Vietinghoff S, Schmitt R, Bräsen JH, Schiffer L, Gerstenberg J, Vijayan V, Dittrich-Breiholz O, Wang L, Karsten CM, Gwinner W, Haller H, Immenschuh S, Gueler F
Frontiers in immunology 2020;11:1204
Frontiers in immunology 2020;11:1204
Pituitary Adenylate Cyclase-Activating Polypeptide Alleviates Intestinal, Extra-Intestinal and Systemic Inflammatory Responses during Acute Campylobacter jejuni-induced Enterocolitis in Mice.
Heimesaat MM, Mousavi S, Kløve S, Genger C, Weschka D, Tamas A, Reglodi D, Bereswill S
Pathogens (Basel, Switzerland) 2020 Sep 30;9(10)
Pathogens (Basel, Switzerland) 2020 Sep 30;9(10)
The Host-Specific Intestinal Microbiota Composition Impacts Campylobacter coli Infection in a Clinical Mouse Model of Campylobacteriosis.
Heimesaat MM, Genger C, Klove S, Weschka D, Mousavi S, Bereswill S
Pathogens (Basel, Switzerland) 2020 Sep 29;9(10)
Pathogens (Basel, Switzerland) 2020 Sep 29;9(10)
The lysophospholipase D enzyme Gdpd3 is required to maintain chronic myelogenous leukaemia stem cells.
Naka K, Ochiai R, Matsubara E, Kondo C, Yang KM, Hoshii T, Araki M, Araki K, Sotomaru Y, Sasaki K, Mitani K, Kim DW, Ooshima A, Kim SJ
Nature communications 2020 Sep 17;11(1):4681
Nature communications 2020 Sep 17;11(1):4681
Aging-Affected MSC Functions and Severity of Periodontal Tissue Destruction in a Ligature-Induced Mouse Periodontitis Model.
Aung KT, Akiyama K, Kunitomo M, Mun AY, Tosa I, Nguyen HTT, Zhang J, Kohno T, Ono M, Hara ES, Kuboki T
International journal of molecular sciences 2020 Oct 30;21(21)
International journal of molecular sciences 2020 Oct 30;21(21)
Cep55 overexpression promotes genomic instability and tumorigenesis in mice.
Sinha D, Nag P, Nanayakkara D, Duijf PHG, Burgess A, Raninga P, Smits VAJ, Bain AL, Subramanian G, Wall M, Finnie JW, Kalimutho M, Khanna KK
Communications biology 2020 Oct 21;3(1):593
Communications biology 2020 Oct 21;3(1):593
Toll-Like Receptor-4 Is Involved in Mediating Intestinal and Extra-Intestinal Inflammation in Campylobacter coli-Infected Secondary Abiotic IL-10(-/-) Mice.
Kløve S, Genger C, Weschka D, Mousavi S, Bereswill S, Heimesaat MM
Microorganisms 2020 Nov 27;8(12)
Microorganisms 2020 Nov 27;8(12)
Immune-modulatory Properties of the Octapeptide NAP in Campylobacter jejuni Infected Mice Suffering from Acute Enterocolitis.
Heimesaat MM, Mousavi S, Kløve S, Genger C, Weschka D, Giladi E, Bereswill S, Gozes I
Microorganisms 2020 May 26;8(6)
Microorganisms 2020 May 26;8(6)
Toll-Like Receptor-4 Dependent Intestinal and Systemic Sequelae Following Peroral Campylobacter coli Infection of IL10 Deficient Mice Harboring a Human Gut Microbiota.
Kløve S, Genger C, Mousavi S, Weschka D, Bereswill S, Heimesaat MM
Pathogens (Basel, Switzerland) 2020 May 18;9(5)
Pathogens (Basel, Switzerland) 2020 May 18;9(5)
Overcoming Immunological Challenges to Helper-Dependent Adenoviral Vector-Mediated Long-Term CFTR Expression in Mouse Airways.
Cao H, Duan R, Hu J
Genes 2020 May 18;11(5)
Genes 2020 May 18;11(5)
The conundrum of colonization resistance against Campylobacter reloaded: The gut microbota composition in conventional mice does not prevent from Campylobacter coli infection.
Genger C, Kløve S, Mousavi S, Bereswill S, Heimesaat MM
European journal of microbiology & immunology 2020 May 1;10(2):80-90
European journal of microbiology & immunology 2020 May 1;10(2):80-90
Inflammatory osteolysis is regulated by site-specific ISGylation of the scaffold protein NEMO.
Adapala NS, Swarnkar G, Arra M, Shen J, Mbalaviele G, Ke K, Abu-Amer Y
eLife 2020 Mar 23;9
eLife 2020 Mar 23;9
Fucoxanthin Ameliorates Atopic Dermatitis Symptoms by Regulating Keratinocytes and Regulatory Innate Lymphoid Cells.
Natsume C, Aoki N, Aoyama T, Senda K, Matsui M, Ikegami A, Tanaka K, Azuma YT, Fujita T
International journal of molecular sciences 2020 Mar 22;21(6)
International journal of molecular sciences 2020 Mar 22;21(6)
Synergistic factors control kinase-phosphatase organization in B-cells engaged with supported bilayers.
Núñez MF, Wisser K, Veatch SL
Molecular biology of the cell 2020 Mar 19;31(7):667-682
Molecular biology of the cell 2020 Mar 19;31(7):667-682
Secreted Phospholipase A(2) Group X Acts as an Adjuvant for Type 2 Inflammation, Leading to an Allergen-Specific Immune Response in the Lung.
Ogden HL, Lai Y, Nolin JD, An D, Frevert CW, Gelb MH, Altemeier WA, Hallstrand TS
Journal of immunology (Baltimore, Md. : 1950) 2020 Jun 15;204(12):3097-3107
Journal of immunology (Baltimore, Md. : 1950) 2020 Jun 15;204(12):3097-3107
Inflammation shapes pathogenesis of murine arrhythmogenic cardiomyopathy.
Lubos N, van der Gaag S, Gerçek M, Kant S, Leube RE, Krusche CA
Basic research in cardiology 2020 Jun 12;115(4):42
Basic research in cardiology 2020 Jun 12;115(4):42
TIAM2S Mediates Serotonin Homeostasis and Provokes a Pro-Inflammatory Immune Microenvironment Permissive for Colorectal Tumorigenesis.
Chan YL, Lai WC, Chen JS, Tseng JT, Chuang PC, Jou J, Lee CT, Sun HS
Cancers 2020 Jul 8;12(7)
Cancers 2020 Jul 8;12(7)
Microbiota composition and inflammatory immune responses upon peroral application of the commercial competitive exclusion product Aviguard® to microbiota-depleted wildtype mice.
Heimesaat MM, Weschka D, Kløve S, Genger C, Biesemeier N, Mousavi S, Bereswill S
European journal of microbiology & immunology 2020 Jul 27;10(3):139-146
European journal of microbiology & immunology 2020 Jul 27;10(3):139-146
Inflammatory Immune Responses and Gut Microbiota Changes Following Campylobacter coli Infection of IL-10(-/-) Mice with Chronic Colitis.
Heimesaat MM, Genger C, Biesemeier N, Klove S, Weschka D, Mousavi S, Bereswill S
Pathogens (Basel, Switzerland) 2020 Jul 11;9(7)
Pathogens (Basel, Switzerland) 2020 Jul 11;9(7)
B cells are sufficient to prime the dominant CD4+ Tfh response to Plasmodium infection.
Arroyo EN, Pepper M
The Journal of experimental medicine 2020 Feb 3;217(2)
The Journal of experimental medicine 2020 Feb 3;217(2)
T-B Lymphocyte Interactions Promote Type 1 Diabetes Independently of SLAM-Associated Protein.
Bonami RH, Nyhoff LE, McNitt DH, Hulbert C, Felton JL, Kendall PL, Thomas JW
Journal of immunology (Baltimore, Md. : 1950) 2020 Dec 15;205(12):3263-3276
Journal of immunology (Baltimore, Md. : 1950) 2020 Dec 15;205(12):3263-3276
New insights on repeated acoustic injury: Augmentation of cochlear susceptibility and inflammatory reaction resultant of prior acoustic injury.
Zhang C, Frye MD, Sun W, Sharma A, Manohar S, Salvi R, Hu BH
Hearing research 2020 Aug;393:107996
Hearing research 2020 Aug;393:107996
MYC Instructs and Maintains Pancreatic Adenocarcinoma Phenotype.
Sodir NM, Kortlever RM, Barthet VJA, Campos T, Pellegrinet L, Kupczak S, Anastasiou P, Swigart LB, Soucek L, Arends MJ, Littlewood TD, Evan GI
Cancer discovery 2020 Apr;10(4):588-607
Cancer discovery 2020 Apr;10(4):588-607
Immunopathological properties of the Campylobacter jejuni flagellins and the adhesin CadF as assessed in a clinical murine infection model.
Schmidt AM, Escher U, Mousavi S, Tegtmeyer N, Boehm M, Backert S, Bereswill S, Heimesaat MM
Gut pathogens 2019;11:24
Gut pathogens 2019;11:24
Inducible IL-7 Hyperexpression Influences Lymphocyte Homeostasis and Function and Increases Allograft Rejection.
Schreiber M, Weigelt M, Karasinsky A, Anastassiadis K, Schallenberg S, Petzold C, Bonifacio E, Kretschmer K, Hommel A
Frontiers in immunology 2019;10:742
Frontiers in immunology 2019;10:742
Tpl2 Protects Against Fulminant Hepatitis Through Mobilization of Myeloid-Derived Suppressor Cells.
Xu J, Pei S, Wang Y, Liu J, Qian Y, Huang M, Zhang Y, Xiao Y
Frontiers in immunology 2019;10:1980
Frontiers in immunology 2019;10:1980
Bacteroides fragilis polysaccharide A induces IL-10 secreting B and T cells that prevent viral encephalitis.
Ramakrishna C, Kujawski M, Chu H, Li L, Mazmanian SK, Cantin EM
Nature communications 2019 May 14;10(1):2153
Nature communications 2019 May 14;10(1):2153
Cellular localization and regulation of receptors and enzymes of the endocannabinoid system in intestinal and systemic inflammation.
Grill M, Hasenoehrl C, Kienzl M, Kargl J, Schicho R
Histochemistry and cell biology 2019 Jan;151(1):5-20
Histochemistry and cell biology 2019 Jan;151(1):5-20
Inhibition of T cell activation and function by the adaptor protein CIN85.
Kong MS, Hashimoto-Tane A, Kawashima Y, Sakuma M, Yokosuka T, Kometani K, Onishi R, Carpino N, Ohara O, Kurosaki T, Phua KK, Saito T
Science signaling 2019 Feb 5;12(567)
Science signaling 2019 Feb 5;12(567)
Aging Induces an Nlrp3 Inflammasome-Dependent Expansion of Adipose B Cells That Impairs Metabolic Homeostasis.
Camell CD, Günther P, Lee A, Goldberg EL, Spadaro O, Youm YH, Bartke A, Hubbard GB, Ikeno Y, Ruddle NH, Schultze J, Dixit VD
Cell metabolism 2019 Dec 3;30(6):1024-1039.e6
Cell metabolism 2019 Dec 3;30(6):1024-1039.e6
Syndecan 1 is a critical mediator of macropinocytosis in pancreatic cancer.
Yao W, Rose JL, Wang W, Seth S, Jiang H, Taguchi A, Liu J, Yan L, Kapoor A, Hou P, Chen Z, Wang Q, Nezi L, Xu Z, Yao J, Hu B, Pettazzoni PF, Ho IL, Feng N, Ramamoorthy V, Jiang S, Deng P, Ma GJ, Den P, Tan Z, Zhang SX, Wang H, Wang YA, Deem AK, Fleming JB, Carugo A, Heffernan TP, Maitra A, Viale A, Ying H, Hanash S, DePinho RA, Draetta GF
Nature 2019 Apr;568(7752):410-414
Nature 2019 Apr;568(7752):410-414
Bone marrow dendritic cells regulate hematopoietic stem/progenitor cell trafficking.
Zhang J, Supakorndej T, Krambs JR, Rao M, Abou-Ezzi G, Ye RY, Li S, Trinkaus K, Link DC
The Journal of clinical investigation 2019 Apr 30;129(7):2920-2931
The Journal of clinical investigation 2019 Apr 30;129(7):2920-2931
The Hematopoietic Oxidase NOX2 Regulates Self-Renewal of Leukemic Stem Cells.
Adane B, Ye H, Khan N, Pei S, Minhajuddin M, Stevens BM, Jones CL, D'Alessandro A, Reisz JA, Zaberezhnyy V, Gasparetto M, Ho TC, Kelly KK, Myers JR, Ashton JM, Siegenthaler J, Kume T, Campbell EL, Pollyea DA, Becker MW, Jordan CT
Cell reports 2019 Apr 2;27(1):238-254.e6
Cell reports 2019 Apr 2;27(1):238-254.e6
Anti-commensal IgG Drives Intestinal Inflammation and Type 17 Immunity in Ulcerative Colitis.
Castro-Dopico T, Dennison TW, Ferdinand JR, Mathews RJ, Fleming A, Clift D, Stewart BJ, Jing C, Strongili K, Labzin LI, Monk EJM, Saeb-Parsy K, Bryant CE, Clare S, Parkes M, Clatworthy MR
Immunity 2019 Apr 16;50(4):1099-1114.e10
Immunity 2019 Apr 16;50(4):1099-1114.e10
Antigen-presenting ILC3 regulate T cell-dependent IgA responses to colonic mucosal bacteria.
Melo-Gonzalez F, Kammoun H, Evren E, Dutton EE, Papadopoulou M, Bradford BM, Tanes C, Fardus-Reid F, Swann JR, Bittinger K, Mabbott NA, Vallance BA, Willinger T, Withers DR, Hepworth MR
The Journal of experimental medicine 2019 Apr 1;216(4):728-742
The Journal of experimental medicine 2019 Apr 1;216(4):728-742
Proapoptotic BIM Impacts B Lymphoid Homeostasis by Limiting the Survival of Mature B Cells in a Cell-Autonomous Manner.
Liu R, King A, Bouillet P, Tarlinton DM, Strasser A, Heierhorst J
Frontiers in immunology 2018;9:592
Frontiers in immunology 2018;9:592
Dysregulated TRAF3 and BCL2 Expression Promotes Multiple Classes of Mature Non-hodgkin B Cell Lymphoma in Mice.
Perez-Chacon G, Adrados M, Vallejo-Cremades MT, Lefebvre S, Reed JC, Zapata JM
Frontiers in immunology 2018;9:3114
Frontiers in immunology 2018;9:3114
ATF3 Sustains IL-22-Induced STAT3 Phosphorylation to Maintain Mucosal Immunity Through Inhibiting Phosphatases.
Glal D, Sudhakar JN, Lu HH, Liu MC, Chiang HY, Liu YC, Cheng CF, Shui JW
Frontiers in immunology 2018;9:2522
Frontiers in immunology 2018;9:2522
Systemic ST6Gal-1 Is a Pro-survival Factor for Murine Transitional B Cells.
Irons EE, Lau JTY
Frontiers in immunology 2018;9:2150
Frontiers in immunology 2018;9:2150
Identification of Distinct Unmutated Chronic Lymphocytic Leukemia Subsets in Mice Based on Their T Cell Dependency.
Pal Singh S, de Bruijn MJW, de Almeida MP, Meijers RWJ, Nitschke L, Langerak AW, Pillai SY, Stadhouders R, Hendriks RW
Frontiers in immunology 2018;9:1996
Frontiers in immunology 2018;9:1996
Tumor-immune profiling of murine syngeneic tumor models as a framework to guide mechanistic studies and predict therapy response in distinct tumor microenvironments.
Yu JW, Bhattacharya S, Yanamandra N, Kilian D, Shi H, Yadavilli S, Katlinskaya Y, Kaczynski H, Conner M, Benson W, Hahn A, Seestaller-Wehr L, Bi M, Vitali NJ, Tsvetkov L, Halsey W, Hughes A, Traini C, Zhou H, Jing J, Lee T, Figueroa DJ, Brett S, Hopson CB, Smothers JF, Hoos A, Srinivasan R
PloS one 2018;13(11):e0206223
PloS one 2018;13(11):e0206223
High-affinity IgM(+) memory B cells are defective in differentiation into IgM antibody-secreting cells by re-stimulation with a T cell-dependent antigen.
Tashiro Y, Murakami A, Hara Y, Shimizu T, Kubo M, Goitsuka R, Kishimoto H, Azuma T
Scientific reports 2018 Sep 28;8(1):14559
Scientific reports 2018 Sep 28;8(1):14559
LaminA/C regulates epigenetic and chromatin architecture changes upon aging of hematopoietic stem cells.
Grigoryan A, Guidi N, Senger K, Liehr T, Soller K, Marka G, Vollmer A, Markaki Y, Leonhardt H, Buske C, Lipka DB, Plass C, Zheng Y, Mulaw MA, Geiger H, Florian MC
Genome biology 2018 Nov 7;19(1):189
Genome biology 2018 Nov 7;19(1):189
Targeted delivery of immune therapeutics to lymph nodes prolongs cardiac allograft survival.
Bahmani B, Uehara M, Jiang L, Ordikhani F, Banouni N, Ichimura T, Solhjou Z, Furtmüller GJ, Brandacher G, Alvarez D, von Andrian UH, Uchimura K, Xu Q, Vohra I, Yilmam OA, Haik Y, Azzi J, Kasinath V, Bromberg JS, McGrath MM, Abdi R
The Journal of clinical investigation 2018 Nov 1;128(11):4770-4786
The Journal of clinical investigation 2018 Nov 1;128(11):4770-4786
Microbial symbionts regulate the primary Ig repertoire.
Chen Y, Chaudhary N, Yang N, Granato A, Turner JA, Howard SL, Devereaux C, Zuo T, Shrestha A, Goel RR, Neuberg D, Wesemann DR
The Journal of experimental medicine 2018 May 7;215(5):1397-1415
The Journal of experimental medicine 2018 May 7;215(5):1397-1415
Establishment of regulatory elements during erythro-megakaryopoiesis identifies hematopoietic lineage-commitment points.
Heuston EF, Keller CA, Lichtenberg J, Giardine B, Anderson SM, NIH Intramural Sequencing Center, Hardison RC, Bodine DM
Epigenetics & chromatin 2018 May 28;11(1):22
Epigenetics & chromatin 2018 May 28;11(1):22
Early cellular interactions and immune transcriptome profiles in human factor VIII-exposed hemophilia A mice.
Lai JD, Cartier D, Hartholt RB, Swystun LL, van Velzen AS, den Haan JMM, Hough C, Voorberg J, Lillicrap D
Journal of thrombosis and haemostasis : JTH 2018 Mar;16(3):533-545
Journal of thrombosis and haemostasis : JTH 2018 Mar;16(3):533-545
A prominent air pollutant, Indeno[1,2,3-cd]pyrene, enhances allergic lung inflammation via aryl hydrocarbon receptor.
Wong TH, Lee CL, Su HH, Lee CL, Wu CC, Wang CC, Sheu CC, Lai RS, Leung SY, Lin CC, Wei YF, Wang CJ, Lin YC, Chen HL, Huang MS, Yen JH, Huang SK, Suen JL
Scientific reports 2018 Mar 26;8(1):5198
Scientific reports 2018 Mar 26;8(1):5198
Host Tumor Suppressor p18(INK4c) Functions as a Potent Cell-Intrinsic Inhibitor of Murine Gammaherpesvirus 68 Reactivation and Pathogenesis.
Niemeyer BF, Oko LM, Medina EM, Oldenburg DG, White DW, Cool CD, Clambey ET, van Dyk LF
Journal of virology 2018 Mar 15;92(6)
Journal of virology 2018 Mar 15;92(6)
Adrenergic nerve degeneration in bone marrow drives aging of the hematopoietic stem cell niche.
Maryanovich M, Zahalka AH, Pierce H, Pinho S, Nakahara F, Asada N, Wei Q, Wang X, Ciero P, Xu J, Leftin A, Frenette PS
Nature medicine 2018 Jun;24(6):782-791
Nature medicine 2018 Jun;24(6):782-791
Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis.
Ziegler PK, Bollrath J, Pallangyo CK, Matsutani T, Canli Ö, De Oliveira T, Diamanti MA, Müller N, Gamrekelashvili J, Putoczki T, Horst D, Mankan AK, Öner MG, Müller S, Müller-Höcker J, Kirchner T, Slotta-Huspenina J, Taketo MM, Reinheckel T, Dröse S, Larner AC, Wels WS, Ernst M, Greten TF, Arkan MC, Korn T, Wirth D, Greten FR
Cell 2018 Jun 28;174(1):88-101.e16
Cell 2018 Jun 28;174(1):88-101.e16
Anti-inflammatory Effects of the Octapeptide NAP in Human Microbiota-Associated Mice Suffering from Subacute Ileitis.
Escher U, Giladi E, Dunay IR, Bereswill S, Gozes I, Heimesaat MM
European journal of microbiology & immunology 2018 Jun 25;8(2):34-40
European journal of microbiology & immunology 2018 Jun 25;8(2):34-40
miR-143/145 differentially regulate hematopoietic stem and progenitor activity through suppression of canonical TGFβ signaling.
Lam J, van den Bosch M, Wegrzyn J, Parker J, Ibrahim R, Slowski K, Chang L, Martinez-Høyer S, Condorelli G, Boldin M, Deng Y, Umlandt P, Fuller M, Karsan A
Nature communications 2018 Jun 20;9(1):2418
Nature communications 2018 Jun 20;9(1):2418
Group 2 Innate Lymphoid Cells Attenuate Inflammatory Arthritis and Protect from Bone Destruction in Mice.
Omata Y, Frech M, Primbs T, Lucas S, Andreev D, Scholtysek C, Sarter K, Kindermann M, Yeremenko N, Baeten DL, Andreas N, Kamradt T, Bozec A, Ramming A, Krönke G, Wirtz S, Schett G, Zaiss MM
Cell reports 2018 Jul 3;24(1):169-180
Cell reports 2018 Jul 3;24(1):169-180
A non-cell-autonomous role for Pml in the maintenance of leukemia from the niche.
Guarnerio J, Mendez LM, Asada N, Menon AV, Fung J, Berry K, Frenette PS, Ito K, Pandolfi PP
Nature communications 2018 Jan 4;9(1):66
Nature communications 2018 Jan 4;9(1):66
Targeting murine leukemic stem cells by antibody functionalized mesoporous silica nanoparticles.
Mandal T, Beck M, Kirsten N, Lindén M, Buske C
Scientific reports 2018 Jan 17;8(1):989
Scientific reports 2018 Jan 17;8(1):989
Oxysterol Sensing through the Receptor GPR183 Promotes the Lymphoid-Tissue-Inducing Function of Innate Lymphoid Cells and Colonic Inflammation.
Emgård J, Kammoun H, García-Cassani B, Chesné J, Parigi SM, Jacob JM, Cheng HW, Evren E, Das S, Czarnewski P, Sleiers N, Melo-Gonzalez F, Kvedaraite E, Svensson M, Scandella E, Hepworth MR, Huber S, Ludewig B, Peduto L, Villablanca EJ, Veiga-Fernandes H, Pereira JP, Flavell RA, Willinger T
Immunity 2018 Jan 16;48(1):120-132.e8
Immunity 2018 Jan 16;48(1):120-132.e8
Tanshinone IIA and Astragaloside IV promote the angiogenesis of mesenchymal stem cell-derived endothelial cell-like cells via upregulation of Cx37, Cx40 and Cx43.
Li Z, Zhang S, Cao L, Li W, Ye YC, Shi ZX, Wang ZR, Sun LX, Wang JW, Jia LT, Wang W
Experimental and therapeutic medicine 2018 Feb;15(2):1847-1854
Experimental and therapeutic medicine 2018 Feb;15(2):1847-1854
Multiple Levels of Control Determine How E4bp4/Nfil3 Regulates NK Cell Development.
Kostrzewski T, Borg AJ, Meng Y, Filipovic I, Male V, Wack A, DiMaggio PA, Brady HJM
Journal of immunology (Baltimore, Md. : 1950) 2018 Feb 15;200(4):1370-1381
Journal of immunology (Baltimore, Md. : 1950) 2018 Feb 15;200(4):1370-1381
Immunomodulatory role of Keratin 76 in oral and gastric cancer.
Sequeira I, Neves JF, Carrero D, Peng Q, Palasz N, Liakath-Ali K, Lord GM, Morgan PR, Lombardi G, Watt FM
Nature communications 2018 Aug 24;9(1):3437
Nature communications 2018 Aug 24;9(1):3437
Inactivation of mTORC1 Signaling in Osterix-Expressing Cells Impairs B-cell Differentiation.
Wang Y, Xiao M, Tao C, Chen J, Wang Z, Yang J, Chen Z, Zou Z, Liu A, Cai D, Jiang Y, Ding C, Li M, Bai X
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2018 Apr;33(4):732-742
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2018 Apr;33(4):732-742
Multidrug-resistant Pseudomonas aeruginosa aggravates inflammatory responses in murine chronic colitis.
von Klitzing E, Ekmekciu I, Kühl AA, Bereswill S, Heimesaat MM
Scientific reports 2018 Apr 27;8(1):6685
Scientific reports 2018 Apr 27;8(1):6685
Absence of the Non-Signalling Chemerin Receptor CCRL2 Exacerbates Acute Inflammatory Responses In Vivo.
Regan-Komito D, Valaris S, Kapellos TS, Recio C, Taylor L, Greaves DR, Iqbal AJ
Frontiers in immunology 2017;8:1621
Frontiers in immunology 2017;8:1621
Blocking CTLA-4 while priming with a whole cell vaccine reshapes the oligoclonal T cell infiltrate and eradicates tumors in an orthotopic glioma model.
Field CS, Hunn MK, Ferguson PM, Ruedl C, Ancelet LR, Hermans IF
Oncoimmunology 2017;7(1):e1376154
Oncoimmunology 2017;7(1):e1376154
The Interleukin (IL)-1R1 pathway is a critical negative regulator of PyMT-mediated mammary tumorigenesis and pulmonary metastasis.
Dagenais M, Dupaul-Chicoine J, Douglas T, Champagne C, Morizot A, Saleh M
Oncoimmunology 2017;6(3):e1287247
Oncoimmunology 2017;6(3):e1287247
Early derivation of IgM memory cells and bone marrow plasmablasts.
Papillion AM, Kenderes KJ, Yates JL, Winslow GM
PloS one 2017;12(6):e0178853
PloS one 2017;12(6):e0178853
Autophagy is a signature of a signaling network that maintains hematopoietic stem cells.
Nguyen-McCarty M, Klein PS
PloS one 2017;12(5):e0177054
PloS one 2017;12(5):e0177054
Multiple functional therapeutic effects of TnP: A small stable synthetic peptide derived from fish venom in a mouse model of multiple sclerosis.
Komegae EN, Souza TA, Grund LZ, Lima C, Lopes-Ferreira M
PloS one 2017;12(2):e0171796
PloS one 2017;12(2):e0171796
Human and Mouse Hematopoietic Stem Cells Are a Depot for Dormant Mycobacterium tuberculosis.
Tornack J, Reece ST, Bauer WM, Vogelzang A, Bandermann S, Zedler U, Stingl G, Kaufmann SH, Melchers F
PloS one 2017;12(1):e0169119
PloS one 2017;12(1):e0169119
Early IL-6 signalling promotes IL-27 dependent maturation of regulatory T cells in the lungs and resolution of viral immunopathology.
Pyle CJ, Uwadiae FI, Swieboda DP, Harker JA
PLoS pathogens 2017 Sep;13(9):e1006640
PLoS pathogens 2017 Sep;13(9):e1006640
Polymicrobial sepsis impairs bystander recruitment of effector cells to infected skin despite optimal sensing and alarming function of skin resident memory CD8 T cells.
Danahy DB, Anthony SM, Jensen IJ, Hartwig SM, Shan Q, Xue HH, Harty JT, Griffith TS, Badovinac VP
PLoS pathogens 2017 Sep;13(9):e1006569
PLoS pathogens 2017 Sep;13(9):e1006569
DNA-binding of the Tet-transactivator curtails antigen-induced lymphocyte activation in mice.
Ottina E, Peperzak V, Schoeler K, Carrington E, Sgonc R, Pellegrini M, Preston S, Herold MJ, Strasser A, Villunger A
Nature communications 2017 Oct 18;8(1):1028
Nature communications 2017 Oct 18;8(1):1028
The microanatomic segregation of selection by apoptosis in the germinal center.
Mayer CT, Gazumyan A, Kara EE, Gitlin AD, Golijanin J, Viant C, Pai J, Oliveira TY, Wang Q, Escolano A, Medina-Ramirez M, Sanders RW, Nussenzweig MC
Science (New York, N.Y.) 2017 Oct 13;358(6360)
Science (New York, N.Y.) 2017 Oct 13;358(6360)
EZH2 enables germinal centre formation through epigenetic silencing of CDKN1A and an Rb-E2F1 feedback loop.
Béguelin W, Rivas MA, Calvo Fernández MT, Teater M, Purwada A, Redmond D, Shen H, Challman MF, Elemento O, Singh A, Melnick AM
Nature communications 2017 Oct 12;8(1):877
Nature communications 2017 Oct 12;8(1):877
Treatment with Allogenic Mesenchymal Stromal Cells in a Murine Model of Systemic Lupus Erythematosus.
Tani C, Vagnani S, Carli L, Querci F, Kühl AA, Spieckermann S, Cieluch CP, Pacini S, Fazzi R, Mosca M
International journal of stem cells 2017 Nov 30;10(2):160-168
International journal of stem cells 2017 Nov 30;10(2):160-168
Oncostatin M drives intestinal inflammation and predicts response to tumor necrosis factor-neutralizing therapy in patients with inflammatory bowel disease.
West NR, Hegazy AN, Owens BMJ, Bullers SJ, Linggi B, Buonocore S, Coccia M, Görtz D, This S, Stockenhuber K, Pott J, Friedrich M, Ryzhakov G, Baribaud F, Brodmerkel C, Cieluch C, Rahman N, Müller-Newen G, Owens RJ, Kühl AA, Maloy KJ, Plevy SE, Oxford IBD Cohort Investigators, Keshav S, Travis SPL, Powrie F
Nature medicine 2017 May;23(5):579-589
Nature medicine 2017 May;23(5):579-589
Differential cytokine contributions of perivascular haematopoietic stem cell niches.
Asada N, Kunisaki Y, Pierce H, Wang Z, Fernandez NF, Birbrair A, Ma'ayan A, Frenette PS
Nature cell biology 2017 Mar;19(3):214-223
Nature cell biology 2017 Mar;19(3):214-223
Bispecific antibody targets multiple Pseudomonas aeruginosa evasion mechanisms in the lung vasculature.
Thanabalasuriar A, Surewaard BG, Willson ME, Neupane AS, Stover CK, Warrener P, Wilson G, Keller AE, Sellman BR, DiGiandomenico A, Kubes P
The Journal of clinical investigation 2017 Jun 1;127(6):2249-2261
The Journal of clinical investigation 2017 Jun 1;127(6):2249-2261
Functional characterization of the lysosomal membrane protein TMEM192 in mice.
Nguyen TL, Schneppenheim J, Rudnik S, Lüllmann-Rauch R, Bernreuther C, Hermans-Borgmeyer I, Glatzel M, Saftig P, Schröder B
Oncotarget 2017 Jul 4;8(27):43635-43652
Oncotarget 2017 Jul 4;8(27):43635-43652
LRCH1 interferes with DOCK8-Cdc42-induced T cell migration and ameliorates experimental autoimmune encephalomyelitis.
Xu X, Han L, Zhao G, Xue S, Gao Y, Xiao J, Zhang S, Chen P, Wu ZY, Ding J, Hu R, Wei B, Wang H
The Journal of experimental medicine 2017 Jan;214(1):209-226
The Journal of experimental medicine 2017 Jan;214(1):209-226
Th2 responses are primed by skin dendritic cells with distinct transcriptional profiles.
Connor LM, Tang SC, Cognard E, Ochiai S, Hilligan KL, Old SI, Pellefigues C, White RF, Patel D, Smith AA, Eccles DA, Lamiable O, McConnell MJ, Ronchese F
The Journal of experimental medicine 2017 Jan;214(1):125-142
The Journal of experimental medicine 2017 Jan;214(1):125-142
Astaxanthin attenuates total body irradiation-induced hematopoietic system injury in mice via inhibition of oxidative stress and apoptosis.
Xue XL, Han XD, Li Y, Chu XF, Miao WM, Zhang JL, Fan SJ
Stem cell research & therapy 2017 Jan 23;8(1):7
Stem cell research & therapy 2017 Jan 23;8(1):7
The podoplanin-CLEC-2 axis inhibits inflammation in sepsis.
Rayes J, Lax S, Wichaiyo S, Watson SK, Di Y, Lombard S, Grygielska B, Smith SW, Skordilis K, Watson SP
Nature communications 2017 Dec 21;8(1):2239
Nature communications 2017 Dec 21;8(1):2239
Alternative activation generates IL-10 producing type 2 innate lymphoid cells.
Seehus CR, Kadavallore A, Torre B, Yeckes AR, Wang Y, Tang J, Kaye J
Nature communications 2017 Dec 1;8(1):1900
Nature communications 2017 Dec 1;8(1):1900
Differential Role of B Cells and IL-17 Versus IFN-γ During Early and Late Rejection of Pig Islet Xenografts in Mice.
Kang HK, Wang S, Dangi A, Zhang X, Singh A, Zhang L, Rosati JM, Suarez-Pinzon W, Deng X, Chen X, Thorp EB, Hering BJ, Miller SD, Luo X
Transplantation 2017 Aug;101(8):1801-1810
Transplantation 2017 Aug;101(8):1801-1810
Irgm1 coordinately regulates autoimmunity and host defense at select mucosal surfaces.
Azzam KM, Madenspacher JH, Cain DW, Lai L, Gowdy KM, Rai P, Janardhan K, Clayton N, Cunningham W, Jensen H, Patel PS, Kearney JF, Taylor GA, Fessler MB
JCI insight 2017 Aug 17;2(16)
JCI insight 2017 Aug 17;2(16)
Epithelial Fli1 deficiency drives systemic autoimmunity and fibrosis: Possible roles in scleroderma.
Takahashi T, Asano Y, Sugawara K, Yamashita T, Nakamura K, Saigusa R, Ichimura Y, Toyama T, Taniguchi T, Akamata K, Noda S, Yoshizaki A, Tsuruta D, Trojanowska M, Sato S
The Journal of experimental medicine 2017 Apr 3;214(4):1129-1151
The Journal of experimental medicine 2017 Apr 3;214(4):1129-1151
Combined antiangiogenic and anti-PD-L1 therapy stimulates tumor immunity through HEV formation.
Allen E, Jabouille A, Rivera LB, Lodewijckx I, Missiaen R, Steri V, Feyen K, Tawney J, Hanahan D, Michael IP, Bergers G
Science translational medicine 2017 Apr 12;9(385)
Science translational medicine 2017 Apr 12;9(385)
Orally administered β-glucan attenuates the Th2 response in a model of airway hypersensitivity.
Burg AR, Quigley L, Jones AV, O'Connor GM, Boelte K, McVicar DW, Orr SJ
SpringerPlus 2016;5(1):815
SpringerPlus 2016;5(1):815
PD-1 pathway-mediated regulation of islet-specific CD4(+) T cell subsets in autoimmune diabetes.
Martinov T, Spanier JA, Pauken KE, Fife BT
Immunoendocrinology (Houston, Tex.) 2016;3
Immunoendocrinology (Houston, Tex.) 2016;3
CRISPR-Mediated Triple Knockout of SLAMF1, SLAMF5 and SLAMF6 Supports Positive Signaling Roles in NKT Cell Development.
Huang B, Gomez-Rodriguez J, Preite S, Garrett LJ, Harper UL, Schwartzberg PL
PloS one 2016;11(6):e0156072
PloS one 2016;11(6):e0156072
Proliferation and Recruitment Contribute to Myocardial Macrophage Expansion in Chronic Heart Failure.
Sager HB, Hulsmans M, Lavine KJ, Moreira MB, Heidt T, Courties G, Sun Y, Iwamoto Y, Tricot B, Khan OF, Dahlman JE, Borodovsky A, Fitzgerald K, Anderson DG, Weissleder R, Libby P, Swirski FK, Nahrendorf M
Circulation research 2016 Sep 16;119(7):853-64
Circulation research 2016 Sep 16;119(7):853-64
Reprogramming the Local Lymph Node Microenvironment Promotes Tolerance that Is Systemic and Antigen Specific.
Tostanoski LH, Chiu YC, Gammon JM, Simon T, Andorko JI, Bromberg JS, Jewell CM
Cell reports 2016 Sep 13;16(11):2940-2952
Cell reports 2016 Sep 13;16(11):2940-2952
LYN- and AIRE-mediated tolerance checkpoint defects synergize to trigger organ-specific autoimmunity.
Proekt I, Miller CN, Jeanne M, Fasano KJ, Moon JJ, Lowell CA, Gould DB, Anderson MS, DeFranco AL
The Journal of clinical investigation 2016 Oct 3;126(10):3758-3771
The Journal of clinical investigation 2016 Oct 3;126(10):3758-3771
M-CSF improves protection against bacterial and fungal infections after hematopoietic stem/progenitor cell transplantation.
Kandalla PK, Sarrazin S, Molawi K, Berruyer C, Redelberger D, Favel A, Bordi C, de Bentzmann S, Sieweke MH
The Journal of experimental medicine 2016 Oct 17;213(11):2269-2279
The Journal of experimental medicine 2016 Oct 17;213(11):2269-2279
Blood Stem Cell Activity Is Arrested by Th1-Mediated Injury Preventing Engraftment following Nonmyeloablative Conditioning.
Müller AM, Florek M, Kohrt HE, Küpper NJ, Filatenkov A, Linderman JA, Hadeiba H, Negrin RS, Shizuru JA
Journal of immunology (Baltimore, Md. : 1950) 2016 Nov 15;197(10):4151-4162
Journal of immunology (Baltimore, Md. : 1950) 2016 Nov 15;197(10):4151-4162
Deficiency of base excision repair enzyme NEIL3 drives increased predisposition to autoimmunity.
Massaad MJ, Zhou J, Tsuchimoto D, Chou J, Jabara H, Janssen E, Glauzy S, Olson BG, Morbach H, Ohsumi TK, Schmitz K, Kyriacos M, Kane J, Torisu K, Nakabeppu Y, Notarangelo LD, Chouery E, Megarbane A, Kang PB, Al-Idrissi E, Aldhekri H, Meffre E, Mizui M, Tsokos GC, Manis JP, Al-Herz W, Wallace SS, Geha RS
The Journal of clinical investigation 2016 Nov 1;126(11):4219-4236
The Journal of clinical investigation 2016 Nov 1;126(11):4219-4236
Balancing intestinal and systemic inflammation through cell type-specific expression of the aryl hydrocarbon receptor repressor.
Brandstätter O, Schanz O, Vorac J, König J, Mori T, Maruyama T, Korkowski M, Haarmann-Stemmann T, von Smolinski D, Schultze JL, Abel J, Esser C, Takeyama H, Weighardt H, Förster I
Scientific reports 2016 May 17;6:26091
Scientific reports 2016 May 17;6:26091
B-cell survival and development controlled by the coordination of NF-κB family members RelB and cRel.
Almaden JV, Liu YC, Yang E, Otero DC, Birnbaum H, Davis-Turak J, Asagiri M, David M, Goldrath AW, Hoffmann A
Blood 2016 Mar 10;127(10):1276-86
Blood 2016 Mar 10;127(10):1276-86
Chronic Gastrointestinal Nematode Infection Mutes Immune Responses to Mycobacterial Infection Distal to the Gut.
Obieglo K, Feng X, Bollampalli VP, Dellacasa-Lindberg I, Classon C, Österblad M, Helmby H, Hewitson JP, Maizels RM, Gigliotti Rothfuchs A, Nylén S
Journal of immunology (Baltimore, Md. : 1950) 2016 Mar 1;196(5):2262-71
Journal of immunology (Baltimore, Md. : 1950) 2016 Mar 1;196(5):2262-71
TNF and CD28 Signaling Play Unique but Complementary Roles in the Systemic Recruitment of Innate Immune Cells after Staphylococcus aureus Enterotoxin A Inhalation.
Svedova J, Tsurutani N, Liu W, Khanna KM, Vella AT
Journal of immunology (Baltimore, Md. : 1950) 2016 Jun 1;196(11):4510-21
Journal of immunology (Baltimore, Md. : 1950) 2016 Jun 1;196(11):4510-21
Bmp signaling in colonic mesenchyme regulates stromal microenvironment and protects from polyposis initiation.
Allaire JM, Roy SA, Ouellet C, Lemieux É, Jones C, Paquet M, Boudreau F, Perreault N
International journal of cancer 2016 Jun 1;138(11):2700-12
International journal of cancer 2016 Jun 1;138(11):2700-12
Group 2 innate lymphoid cells utilize the IRF4-IL-9 module to coordinate epithelial cell maintenance of lung homeostasis.
Mohapatra A, Van Dyken SJ, Schneider C, Nussbaum JC, Liang HE, Locksley RM
Mucosal immunology 2016 Jan;9(1):275-86
Mucosal immunology 2016 Jan;9(1):275-86
The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells.
Saulep-Easton D, Vincent FB, Quah PS, Wei A, Ting SB, Croce CM, Tam C, Mackay F
Leukemia 2016 Jan;30(1):163-72
Leukemia 2016 Jan;30(1):163-72
A20 Deficiency in Lung Epithelial Cells Protects against Influenza A Virus Infection.
Maelfait J, Roose K, Vereecke L, Mc Guire C, Sze M, Schuijs MJ, Willart M, Ibañez LI, Hammad H, Lambrecht BN, Beyaert R, Saelens X, van Loo G
PLoS pathogens 2016 Jan;12(1):e1005410
PLoS pathogens 2016 Jan;12(1):e1005410
Postnatal lethality and abnormal development of foregut and spleen in Ndrg4 mutant mice.
Qu X, Li J, Baldwin HS
Biochemical and biophysical research communications 2016 Feb 12;470(3):613-619
Biochemical and biophysical research communications 2016 Feb 12;470(3):613-619
miR-150 regulates obesity-associated insulin resistance by controlling B cell functions.
Ying W, Tseng A, Chang RC, Wang H, Lin YL, Kanameni S, Brehm T, Morin A, Jones B, Splawn T, Criscitiello M, Golding MC, Bazer FW, Safe S, Zhou B
Scientific reports 2016 Feb 1;6:20176
Scientific reports 2016 Feb 1;6:20176
This paper is the winner of an SFB Award in the Hospital Intern, Residency category: Peptide biomaterials raising adaptive immune responses in wound healing contexts.
Vigneswaran Y, Han H, De Loera R, Wen Y, Zhang X, Sun T, Mora-Solano C, Collier JH
Journal of biomedical materials research. Part A 2016 Aug;104(8):1853-62
Journal of biomedical materials research. Part A 2016 Aug;104(8):1853-62
Murine iPSC-Derived Macrophages as a Tool for Disease Modeling of Hereditary Pulmonary Alveolar Proteinosis due to Csf2rb Deficiency.
Mucci A, Kunkiel J, Suzuki T, Brennig S, Glage S, Kühnel MP, Ackermann M, Happle C, Kuhn A, Schambach A, Trapnell BC, Hansen G, Moritz T, Lachmann N
Stem cell reports 2016 Aug 9;7(2):292-305
Stem cell reports 2016 Aug 9;7(2):292-305
Resident T Cells Are Unable To Control Herpes Simplex Virus-1 Activity in the Brain Ependymal Region during Latency.
Menendez CM, Jinkins JK, Carr DJ
Journal of immunology (Baltimore, Md. : 1950) 2016 Aug 15;197(4):1262-75
Journal of immunology (Baltimore, Md. : 1950) 2016 Aug 15;197(4):1262-75
Trypanosoma cruzi Causes Paralyzing Systemic Necrotizing Vasculitis Driven by Pathogen-Specific Type I Immunity in Mice.
Roffê E, Marino APMP, Weaver J, Wan W, de Araújo FF, Hoffman V, Santiago HC, Murphy PM
Infection and immunity 2016 Apr;84(4):1123-1136
Infection and immunity 2016 Apr;84(4):1123-1136
Protective Role for B-1b B Cells and IgM in Obesity-Associated Inflammation, Glucose Intolerance, and Insulin Resistance.
Harmon DB, Srikakulapu P, Kaplan JL, Oldham SN, McSkimming C, Garmey JC, Perry HM, Kirby JL, Prohaska TA, Gonen A, Hallowell P, Schirmer B, Tsimikas S, Taylor AM, Witztum JL, McNamara CA
Arteriosclerosis, thrombosis, and vascular biology 2016 Apr;36(4):682-91
Arteriosclerosis, thrombosis, and vascular biology 2016 Apr;36(4):682-91
NFκB1 is essential to prevent the development of multiorgan autoimmunity by limiting IL-6 production in follicular B cells.
de Valle E, Grigoriadis G, O'Reilly LA, Willis SN, Maxwell MJ, Corcoran LM, Tsantikos E, Cornish JK, Fairfax KA, Vasanthakumar A, Febbraio MA, Hibbs ML, Pellegrini M, Banerjee A, Hodgkin PD, Kallies A, Mackay F, Strasser A, Gerondakis S, Gugasyan R
The Journal of experimental medicine 2016 Apr 4;213(4):621-41
The Journal of experimental medicine 2016 Apr 4;213(4):621-41
Type I IFN Does Not Promote Susceptibility to Foodborne Listeria monocytogenes.
Pitts MG, Myers-Morales T, D'Orazio SE
Journal of immunology (Baltimore, Md. : 1950) 2016 Apr 1;196(7):3109-16
Journal of immunology (Baltimore, Md. : 1950) 2016 Apr 1;196(7):3109-16
LNK/SH2B3 regulates IL-7 receptor signaling in normal and malignant B-progenitors.
Cheng Y, Chikwava K, Wu C, Zhang H, Bhagat A, Pei D, Choi JK, Tong W
The Journal of clinical investigation 2016 Apr 1;126(4):1267-81
The Journal of clinical investigation 2016 Apr 1;126(4):1267-81
Vitamin A Impairs the Reprogramming of Tregs into IL-17-Producing Cells during Intestinal Inflammation.
Tejón G, Manríquez V, De Calisto J, Flores-Santibáñez F, Hidalgo Y, Crisóstomo N, Fernández D, Sauma D, Mora JR, Bono MR, Rosemblatt M
BioMed research international 2015;2015:137893
BioMed research international 2015;2015:137893
The immunosenescence-related gene Zizimin2 is associated with early bone marrow B cell development and marginal zone B cell formation.
Matsuda T, Yanase S, Takaoka A, Maruyama M
Immunity & ageing : I & A 2015;12:1
Immunity & ageing : I & A 2015;12:1
Evaluation of the BDCA2-DTR Transgenic Mouse Model in Chronic and Acute Inflammation.
Mandl M, Drechsler M, Jansen Y, Neideck C, Noels H, Faussner A, Soehnlein O, Weber C, Döring Y
PloS one 2015;10(8):e0134176
PloS one 2015;10(8):e0134176
Mitochondrial ATP transporter Ant2 depletion impairs erythropoiesis and B lymphopoiesis.
Cho J, Seo J, Lim CH, Yang L, Shiratsuchi T, Lee MH, Chowdhury RR, Kasahara H, Kim JS, Oh SP, Lee YJ, Terada N
Cell death and differentiation 2015 Sep;22(9):1437-50
Cell death and differentiation 2015 Sep;22(9):1437-50
Inflammation rapidly reorganizes mouse bone marrow B cells and their environment in conjunction with early IgM responses.
Moreau JM, Berger A, Nelles ME, Mielnik M, Furlonger C, Cen SY, Besla R, Robbins CS, Paige CJ
Blood 2015 Sep 3;126(10):1184-92
Blood 2015 Sep 3;126(10):1184-92
Metformin ameliorates ionizing irradiation-induced long-term hematopoietic stem cell injury in mice.
Xu G, Wu H, Zhang J, Li D, Wang Y, Wang Y, Zhang H, Lu L, Li C, Huang S, Xing Y, Zhou D, Meng A
Free radical biology & medicine 2015 Oct;87:15-25
Free radical biology & medicine 2015 Oct;87:15-25
Endothelial Gata5 transcription factor regulates blood pressure.
Messaoudi S, He Y, Gutsol A, Wight A, Hébert RL, Vilmundarson RO, Makrigiannis AP, Chalmers J, Hamet P, Tremblay J, McPherson R, Stewart AFR, Touyz RM, Nemer M
Nature communications 2015 Nov 30;6:8835
Nature communications 2015 Nov 30;6:8835
mTORC1 and mTORC2 selectively regulate CD8⁺ T cell differentiation.
Pollizzi KN, Patel CH, Sun IH, Oh MH, Waickman AT, Wen J, Delgoffe GM, Powell JD
The Journal of clinical investigation 2015 May;125(5):2090-108
The Journal of clinical investigation 2015 May;125(5):2090-108
Limited miR-17-92 overexpression drives hematologic malignancies.
Danielson LS, Reavie L, Coussens M, Davalos V, Castillo-Martin M, Guijarro MV, Coffre M, Cordon-Cardo C, Aifantis I, Ibrahim S, Liu C, Koralov SB, Hernando E
Leukemia research 2015 Mar;39(3):335-41
Leukemia research 2015 Mar;39(3):335-41
Enhanced CD8 T cell responses through GITR-mediated costimulation resolve chronic viral infection.
Pascutti MF, Geerman S, Slot E, van Gisbergen KP, Boon L, Arens R, van Lier RA, Wolkers MC, Nolte MA
PLoS pathogens 2015 Mar;11(3):e1004675
PLoS pathogens 2015 Mar;11(3):e1004675
LKB1 inhibition of NF-κB in B cells prevents T follicular helper cell differentiation and germinal center formation.
Walsh NC, Waters LR, Fowler JA, Lin M, Cunningham CR, Brooks DG, Rehg JE, Morse HC 3rd, Teitell MA
EMBO reports 2015 Jun;16(6):753-68
EMBO reports 2015 Jun;16(6):753-68
Peptide-MHC-I from Endogenous Antigen Outnumber Those from Exogenous Antigen, Irrespective of APC Phenotype or Activation.
Sei JJ, Haskett S, Kaminsky LW, Lin E, Truckenmiller ME, Bellone CJ, Buller RM, Norbury CC
PLoS pathogens 2015 Jun;11(6):e1004941
PLoS pathogens 2015 Jun;11(6):e1004941
Disruption of p21-activated kinase 1 gene diminishes atherosclerosis in apolipoprotein E-deficient mice.
Singh NK, Kotla S, Dyukova E, Traylor JG Jr, Orr AW, Chernoff J, Marion TN, Rao GN
Nature communications 2015 Jun 24;6:7450
Nature communications 2015 Jun 24;6:7450
SOCS3 Deficiency in Myeloid Cells Promotes Tumor Development: Involvement of STAT3 Activation and Myeloid-Derived Suppressor Cells.
Yu H, Liu Y, McFarland BC, Deshane JS, Hurst DR, Ponnazhagan S, Benveniste EN, Qin H
Cancer immunology research 2015 Jul;3(7):727-40
Cancer immunology research 2015 Jul;3(7):727-40
Control of signaling-mediated clearance of apoptotic cells by the tumor suppressor p53.
Yoon KW, Byun S, Kwon E, Hwang SY, Chu K, Hiraki M, Jo SH, Weins A, Hakroush S, Cebulla A, Sykes DB, Greka A, Mundel P, Fisher DE, Mandinova A, Lee SW
Science (New York, N.Y.) 2015 Jul 31;349(6247):1261669
Science (New York, N.Y.) 2015 Jul 31;349(6247):1261669
Structural and functional features of central nervous system lymphatic vessels.
Louveau A, Smirnov I, Keyes TJ, Eccles JD, Rouhani SJ, Peske JD, Derecki NC, Castle D, Mandell JW, Lee KS, Harris TH, Kipnis J
Nature 2015 Jul 16;523(7560):337-41
Nature 2015 Jul 16;523(7560):337-41
Comparison of tamoxifen and letrozole response in mammary preneoplasia of ER and aromatase overexpressing mice defines an immune-associated gene signature linked to tamoxifen resistance.
Dabydeen SA, Kang K, Díaz-Cruz ES, Alamri A, Axelrod ML, Bouker KB, Al-Kharboosh R, Clarke R, Hennighausen L, Furth PA
Carcinogenesis 2015 Jan;36(1):122-32
Carcinogenesis 2015 Jan;36(1):122-32
The CLEC-2-podoplanin axis controls the contractility of fibroblastic reticular cells and lymph node microarchitecture.
Astarita JL, Cremasco V, Fu J, Darnell MC, Peck JR, Nieves-Bonilla JM, Song K, Kondo Y, Woodruff MC, Gogineni A, Onder L, Ludewig B, Weimer RM, Carroll MC, Mooney DJ, Xia L, Turley SJ
Nature immunology 2015 Jan;16(1):75-84
Nature immunology 2015 Jan;16(1):75-84
CCR7-dependent trafficking of RORγ⁺ ILCs creates a unique microenvironment within mucosal draining lymph nodes.
Mackley EC, Houston S, Marriott CL, Halford EE, Lucas B, Cerovic V, Filbey KJ, Maizels RM, Hepworth MR, Sonnenberg GF, Milling S, Withers DR
Nature communications 2015 Jan 9;6:5862
Nature communications 2015 Jan 9;6:5862
F-box protein FBXW7 inhibits cancer metastasis in a non-cell-autonomous manner.
Yumimoto K, Akiyoshi S, Ueo H, Sagara Y, Onoyama I, Ueo H, Ohno S, Mori M, Mimori K, Nakayama KI
The Journal of clinical investigation 2015 Feb;125(2):621-35
The Journal of clinical investigation 2015 Feb;125(2):621-35
Role of TP53 mutations in the origin and evolution of therapy-related acute myeloid leukaemia.
Wong TN, Ramsingh G, Young AL, Miller CA, Touma W, Welch JS, Lamprecht TL, Shen D, Hundal J, Fulton RS, Heath S, Baty JD, Klco JM, Ding L, Mardis ER, Westervelt P, DiPersio JF, Walter MJ, Graubert TA, Ley TJ, Druley T, Link DC, Wilson RK
Nature 2015 Feb 26;518(7540):552-555
Nature 2015 Feb 26;518(7540):552-555
Small-molecule inhibitors targeting INK4 protein p18(INK4C) enhance ex vivo expansion of haematopoietic stem cells.
Gao Y, Yang P, Shen H, Yu H, Song X, Zhang L, Zhang P, Cheng H, Xie Z, Hao S, Dong F, Ma S, Ji Q, Bartlow P, Ding Y, Wang L, Liu H, Li Y, Cheng H, Miao W, Yuan W, Yuan Y, Cheng T, Xie XQ
Nature communications 2015 Feb 18;6:6328
Nature communications 2015 Feb 18;6:6328
The E-Id protein axis modulates the activities of the PI3K-AKT-mTORC1-Hif1a and c-myc/p19Arf pathways to suppress innate variant TFH cell development, thymocyte expansion, and lymphomagenesis.
Miyazaki M, Miyazaki K, Chen S, Chandra V, Wagatsuma K, Agata Y, Rodewald HR, Saito R, Chang AN, Varki N, Kawamoto H, Murre C
Genes & development 2015 Feb 15;29(4):409-25
Genes & development 2015 Feb 15;29(4):409-25
ELF5 Drives Lung Metastasis in Luminal Breast Cancer through Recruitment of Gr1+ CD11b+ Myeloid-Derived Suppressor Cells.
Gallego-Ortega D, Ledger A, Roden DL, Law AM, Magenau A, Kikhtyak Z, Cho C, Allerdice SL, Lee HJ, Valdes-Mora F, Herrmann D, Salomon R, Young AI, Lee BY, Sergio CM, Kaplan W, Piggin C, Conway JR, Rabinovich B, Millar EK, Oakes SR, Chtanova T, Swarbrick A, Naylor MJ, O'Toole S, Green AR, Timpson P, Gee JM, Ellis IO, Clark SJ, Ormandy CJ
PLoS biology 2015 Dec;13(12):e1002330
PLoS biology 2015 Dec;13(12):e1002330
Aging-associated inflammation promotes selection for adaptive oncogenic events in B cell progenitors.
Henry CJ, Casás-Selves M, Kim J, Zaberezhnyy V, Aghili L, Daniel AE, Jimenez L, Azam T, McNamee EN, Clambey ET, Klawitter J, Serkova NJ, Tan AC, Dinarello CA, DeGregori J
The Journal of clinical investigation 2015 Dec;125(12):4666-80
The Journal of clinical investigation 2015 Dec;125(12):4666-80
MHC-mismatched mixed chimerism augments thymic regulatory T-cell production and prevents relapse of EAE in mice.
Wu L, Li N, Zhang M, Xue SL, Cassady K, Lin Q, Riggs AD, Zeng D
Proceedings of the National Academy of Sciences of the United States of America 2015 Dec 29;112(52):15994-9
Proceedings of the National Academy of Sciences of the United States of America 2015 Dec 29;112(52):15994-9
Depletion of B220(+)NK1.1(+) cells enhances the rejection of established melanoma by tumor-specific CD4(+) T cells.
Wilson KA, Goding SR, Neely HR, Harris KM, Antony PA
Oncoimmunology 2015 Aug;4(8):e1019196
Oncoimmunology 2015 Aug;4(8):e1019196
TNF signals are dispensable for the generation of CD23+ CD21/35-high CD1d-high B cells in inflamed lymph nodes.
Kuzin II, Bouta EM, Schwarz EM, Bottaro A
Cellular immunology 2015 Aug;296(2):133-7
Cellular immunology 2015 Aug;296(2):133-7
Deficient Natural Killer Dendritic Cell Responses Underlay the Induction of Theiler's Virus-Induced Autoimmunity.
Chastain EM, Getts DR, Miller SD
mBio 2015 Aug 4;6(4):e01175
mBio 2015 Aug 4;6(4):e01175
Kindlin-3-mediated integrin adhesion is dispensable for quiescent but essential for activated hematopoietic stem cells.
Ruppert R, Moser M, Sperandio M, Rognoni E, Orban M, Liu WH, Schulz AS, Oostendorp RA, Massberg S, Fässler R
The Journal of experimental medicine 2015 Aug 24;212(9):1415-32
The Journal of experimental medicine 2015 Aug 24;212(9):1415-32
Serotonin and insulin-like peptides modulate leucokinin-producing neurons that affect feeding and water homeostasis in Drosophila.
Liu Y, Luo J, Carlsson MA, Nässel DR
The Journal of comparative neurology 2015 Aug 15;523(12):1840-63
The Journal of comparative neurology 2015 Aug 15;523(12):1840-63
Splenic red pulp macrophages are intrinsically superparamagnetic and contaminate magnetic cell isolates.
Franken L, Klein M, Spasova M, Elsukova A, Wiedwald U, Welz M, Knolle P, Farle M, Limmer A, Kurts C
Scientific reports 2015 Aug 11;5:12940
Scientific reports 2015 Aug 11;5:12940
Setd1a regulates progenitor B-cell-to-precursor B-cell development through histone H3 lysine 4 trimethylation and Ig heavy-chain rearrangement.
Tusi BK, Deng C, Salz T, Zeumer L, Li Y, So CW, Morel LM, Qiu Y, Huang S
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2015 Apr;29(4):1505-15
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2015 Apr;29(4):1505-15
Identification of Toll-like receptor 9 as parapoxvirus ovis-sensing receptor in plasmacytoid dendritic cells.
von Buttlar H, Siegemund S, Büttner M, Alber G
PloS one 2014;9(8):e106188
PloS one 2014;9(8):e106188
Nfatc2 and Tob1 have non-overlapping function in T cell negative regulation and tumorigenesis.
May SL, Zhou Q, Lewellen M, Carter CM, Coffey D, Highfill SL, Bucher CM, Matise I, Morse HC 3rd, O'Sullivan MG, Schutten M, Johnson C, Bellgrau D, Blazar BR, Modiano JF
PloS one 2014;9(6):e100629
PloS one 2014;9(6):e100629
Purinergic signaling on leukocytes infiltrating the LPS-injured lung.
Friebe D, Yang T, Schmidt T, Borg N, Steckel B, Ding Z, Schrader J
PloS one 2014;9(4):e95382
PloS one 2014;9(4):e95382
Activated macrophage survival is coordinated by TAK1 binding proteins.
Mihaly SR, Morioka S, Ninomiya-Tsuji J, Takaesu G
PloS one 2014;9(4):e94982
PloS one 2014;9(4):e94982
B-cell-intrinsic hepatitis C virus expression leads to B-cell-lymphomagenesis and induction of NF-κB signalling.
Kasama Y, Mizukami T, Kusunoki H, Peveling-Oberhag J, Nishito Y, Ozawa M, Kohara M, Mizuochi T, Tsukiyama-Kohara K
PloS one 2014;9(3):e91373
PloS one 2014;9(3):e91373
Administration of the resveratrol analogues isorhapontigenin and heyneanol-A protects mice hematopoietic cells against irradiation injuries.
Wang H, Yang YL, Zhang H, Yan H, Wu XJ, Zhang CZ
BioMed research international 2014;2014:282657
BioMed research international 2014;2014:282657
Antigen signal strength during priming determines effector CD4 T cell function and antigen sensitivity during influenza virus challenge.
Nagaoka M, Hatta Y, Kawaoka Y, Malherbe LP
Journal of immunology (Baltimore, Md. : 1950) 2014 Sep 15;193(6):2812-20
Journal of immunology (Baltimore, Md. : 1950) 2014 Sep 15;193(6):2812-20
Thymic epithelium determines a spontaneous chronic neuritis in Icam1(tm1Jcgr)NOD mice.
Meyer zu Horste G, Mausberg AK, Cordes S, El-Haddad H, Partke HJ, Leussink VI, Roden M, Martin S, Steinman L, Hartung HP, Kieseier BC
Journal of immunology (Baltimore, Md. : 1950) 2014 Sep 15;193(6):2678-90
Journal of immunology (Baltimore, Md. : 1950) 2014 Sep 15;193(6):2678-90
A NOTCH1-driven MYC enhancer promotes T cell development, transformation and acute lymphoblastic leukemia.
Herranz D, Ambesi-Impiombato A, Palomero T, Schnell SA, Belver L, Wendorff AA, Xu L, Castillo-Martin M, Llobet-Navás D, Cordon-Cardo C, Clappier E, Soulier J, Ferrando AA
Nature medicine 2014 Oct;20(10):1130-7
Nature medicine 2014 Oct;20(10):1130-7
CD28 expression is required after T cell priming for helper T cell responses and protective immunity to infection.
Linterman MA, Denton AE, Divekar DP, Zvetkova I, Kane L, Ferreira C, Veldhoen M, Clare S, Dougan G, Espéli M, Smith KG
eLife 2014 Oct 27;3
eLife 2014 Oct 27;3
Progressive replacement of embryo-derived cardiac macrophages with age.
Molawi K, Wolf Y, Kandalla PK, Favret J, Hagemeyer N, Frenzel K, Pinto AR, Klapproth K, Henri S, Malissen B, Rodewald HR, Rosenthal NA, Bajenoff M, Prinz M, Jung S, Sieweke MH
The Journal of experimental medicine 2014 Oct 20;211(11):2151-8
The Journal of experimental medicine 2014 Oct 20;211(11):2151-8
Cutting edge: CD8+ recent thymic emigrants exhibit increased responses to low-affinity ligands and improved access to peripheral sites of inflammation.
Berkley AM, Fink PJ
Journal of immunology (Baltimore, Md. : 1950) 2014 Oct 1;193(7):3262-6
Journal of immunology (Baltimore, Md. : 1950) 2014 Oct 1;193(7):3262-6
Internalization and TLR-dependent type I interferon production by monocytes in response to Toxoplasma gondii.
Han SJ, Melichar HJ, Coombes JL, Chan SW, Koshy AA, Boothroyd JC, Barton GM, Robey EA
Immunology and cell biology 2014 Nov;92(10):872-81
Immunology and cell biology 2014 Nov;92(10):872-81
INAM plays a critical role in IFN-γ production by NK cells interacting with polyinosinic-polycytidylic acid-stimulated accessory cells.
Kasamatsu J, Azuma M, Oshiumi H, Morioka Y, Okabe M, Ebihara T, Matsumoto M, Seya T
Journal of immunology (Baltimore, Md. : 1950) 2014 Nov 15;193(10):5199-207
Journal of immunology (Baltimore, Md. : 1950) 2014 Nov 15;193(10):5199-207
Lasting antibody responses are mediated by a combination of newly formed and established bone marrow plasma cells drawn from clonally distinct precursors.
Chernova I, Jones DD, Wilmore JR, Bortnick A, Yucel M, Hershberg U, Allman D
Journal of immunology (Baltimore, Md. : 1950) 2014 Nov 15;193(10):4971-9
Journal of immunology (Baltimore, Md. : 1950) 2014 Nov 15;193(10):4971-9
Transcription elongation factor ELL2 drives Ig secretory-specific mRNA production and the unfolded protein response.
Park KS, Bayles I, Szlachta-McGinn A, Paul J, Boiko J, Santos P, Liu J, Wang Z, Borghesi L, Milcarek C
Journal of immunology (Baltimore, Md. : 1950) 2014 Nov 1;193(9):4663-74
Journal of immunology (Baltimore, Md. : 1950) 2014 Nov 1;193(9):4663-74
Costimulation via the tumor-necrosis factor receptor superfamily couples TCR signal strength to the thymic differentiation of regulatory T cells.
Mahmud SA, Manlove LS, Schmitz HM, Xing Y, Wang Y, Owen DL, Schenkel JM, Boomer JS, Green JM, Yagita H, Chi H, Hogquist KA, Farrar MA
Nature immunology 2014 May;15(5):473-81
Nature immunology 2014 May;15(5):473-81
MyD88-dependent immunity to a natural model of vaccinia virus infection does not involve Toll-like receptor 2.
Davies ML, Sei JJ, Siciliano NA, Xu RH, Roscoe F, Sigal LJ, Eisenlohr LC, Norbury CC
Journal of virology 2014 Mar;88(6):3557-67
Journal of virology 2014 Mar;88(6):3557-67
Memory B cells form in aged mice despite impaired affinity maturation and germinal center kinetics.
Goenka R, Scholz JL, Naradikian MS, Cancro MP
Experimental gerontology 2014 Jun;54:109-15
Experimental gerontology 2014 Jun;54:109-15
Combined MEK and JAK inhibition abrogates murine myeloproliferative neoplasm.
Kong G, Wunderlich M, Yang D, Ranheim EA, Young KH, Wang J, Chang YI, Du J, Liu Y, Tey SR, Zhang X, Juckett M, Mattison R, Damnernsawad A, Zhang J, Mulloy JC, Zhang J
The Journal of clinical investigation 2014 Jun;124(6):2762-73
The Journal of clinical investigation 2014 Jun;124(6):2762-73
Intrinsic TGF-β signaling promotes age-dependent CD8+ T cell polyfunctionality attrition.
Bhadra R, Moretto MM, Castillo JC, Petrovas C, Ferrando-Martinez S, Shokal U, Leal M, Koup RA, Eleftherianos I, Khan IA
The Journal of clinical investigation 2014 Jun;124(6):2441-55
The Journal of clinical investigation 2014 Jun;124(6):2441-55
12-Hydroxyheptadecatrienoic acid promotes epidermal wound healing by accelerating keratinocyte migration via the BLT2 receptor.
Liu M, Saeki K, Matsunobu T, Okuno T, Koga T, Sugimoto Y, Yokoyama C, Nakamizo S, Kabashima K, Narumiya S, Shimizu T, Yokomizo T
The Journal of experimental medicine 2014 Jun 2;211(6):1063-78
The Journal of experimental medicine 2014 Jun 2;211(6):1063-78
Ly49 family receptors are required for cancer immunosurveillance mediated by natural killer cells.
Tu MM, Mahmoud AB, Wight A, Mottashed A, Bélanger S, Rahim MM, Abou-Samra E, Makrigiannis AP
Cancer research 2014 Jul 15;74(14):3684-94
Cancer research 2014 Jul 15;74(14):3684-94
Paradoxical resistance to high-fat diet-induced obesity and altered macrophage polarization in mineralocorticoid receptor-overexpressing mice.
Kuhn E, Bourgeois C, Keo V, Viengchareun S, Muscat A, Meduri G, Le Menuet D, Fève B, Lombès M
American journal of physiology. Endocrinology and metabolism 2014 Jan 1;306(1):E75-90
American journal of physiology. Endocrinology and metabolism 2014 Jan 1;306(1):E75-90
Lipoxin A₄ modulates adaptive immunity by decreasing memory B-cell responses via an ALX/FPR2-dependent mechanism.
Ramon S, Bancos S, Serhan CN, Phipps RP
European journal of immunology 2014 Feb;44(2):357-69
European journal of immunology 2014 Feb;44(2):357-69
B cell-specific loss of Lyn kinase leads to autoimmunity.
Lamagna C, Hu Y, DeFranco AL, Lowell CA
Journal of immunology (Baltimore, Md. : 1950) 2014 Feb 1;192(3):919-28
Journal of immunology (Baltimore, Md. : 1950) 2014 Feb 1;192(3):919-28
Central memory CD4+ T cells are responsible for the recombinant Bacillus Calmette-Guérin ΔureC::hly vaccine's superior protection against tuberculosis.
Vogelzang A, Perdomo C, Zedler U, Kuhlmann S, Hurwitz R, Gengenbacher M, Kaufmann SH
The Journal of infectious diseases 2014 Dec 15;210(12):1928-37
The Journal of infectious diseases 2014 Dec 15;210(12):1928-37
Rhof promotes murine marginal zone B cell development.
Kishimoto M, Matsuda T, Yanase S, Katsumi A, Suzuki N, Ikejiri M, Takagi A, Ikawa M, Kojima T, Kunishima S, Kiyoi H, Naoe T, Matsushita T, Maruyama M
Nagoya journal of medical science 2014 Aug;76(3-4):293-305
Nagoya journal of medical science 2014 Aug;76(3-4):293-305
APRIL mediates peritoneal B-1 cell homeostasis.
Sindhava VJ, Scholz JL, Stohl W, Cancro MP
Immunology letters 2014 Aug;160(2):120-7
Immunology letters 2014 Aug;160(2):120-7
In utero depletion of fetal hematopoietic stem cells improves engraftment after neonatal transplantation in mice.
Derderian SC, Togarrati PP, King C, Moradi PW, Reynaud D, Czechowicz A, Weissman IL, MacKenzie TC
Blood 2014 Aug 7;124(6):973-80
Blood 2014 Aug 7;124(6):973-80
Recognition of tumor cells by Dectin-1 orchestrates innate immune cells for anti-tumor responses.
Chiba S, Ikushima H, Ueki H, Yanai H, Kimura Y, Hangai S, Nishio J, Negishi H, Tamura T, Saijo S, Iwakura Y, Taniguchi T
eLife 2014 Aug 22;3:e04177
eLife 2014 Aug 22;3:e04177
The transcription factor E4bp4/Nfil3 controls commitment to the NK lineage and directly regulates Eomes and Id2 expression.
Male V, Nisoli I, Kostrzewski T, Allan DS, Carlyle JR, Lord GM, Wack A, Brady HJ
The Journal of experimental medicine 2014 Apr 7;211(4):635-42
The Journal of experimental medicine 2014 Apr 7;211(4):635-42
IκBε is a key regulator of B cell expansion by providing negative feedback on cRel and RelA in a stimulus-specific manner.
Alves BN, Tsui R, Almaden J, Shokhirev MN, Davis-Turak J, Fujimoto J, Birnbaum H, Ponomarenko J, Hoffmann A
Journal of immunology (Baltimore, Md. : 1950) 2014 Apr 1;192(7):3121-32
Journal of immunology (Baltimore, Md. : 1950) 2014 Apr 1;192(7):3121-32
TDP2-dependent non-homologous end-joining protects against topoisomerase II-induced DNA breaks and genome instability in cells and in vivo.
Gómez-Herreros F, Romero-Granados R, Zeng Z, Alvarez-Quilón A, Quintero C, Ju L, Umans L, Vermeire L, Huylebroeck D, Caldecott KW, Cortés-Ledesma F
PLoS genetics 2013;9(3):e1003226
PLoS genetics 2013;9(3):e1003226
A New Murine Model for Gastrointestinal Anthrax Infection.
Xie T, Sun C, Uslu K, Auth RD, Fang H, Ouyang W, Frucht DM
PloS one 2013;8(6):e66943
PloS one 2013;8(6):e66943
In vivo knockdown of TAK1 accelerates bone marrow proliferation/differentiation and induces systemic inflammation.
Vink PM, Smout WM, Driessen-Engels LJ, de Bruin AM, Delsing D, Krajnc-Franken MA, Jansen AJ, Rovers EF, van Puijenbroek AA, Kaptein A, Nolte MA, Garritsen A, van Eenennaam H
PloS one 2013;8(3):e57348
PloS one 2013;8(3):e57348
Transient B-cell depletion combined with apoptotic donor splenocytes induces xeno-specific T- and B-cell tolerance to islet xenografts.
Wang S, Tasch J, Kheradmand T, Ulaszek J, Ely S, Zhang X, Hering BJ, Miller SD, Luo X
Diabetes 2013 Sep;62(9):3143-50
Diabetes 2013 Sep;62(9):3143-50
COP1 targets C/EBPα for degradation and induces acute myeloid leukemia via Trib1.
Yoshida A, Kato JY, Nakamae I, Yoneda-Kato N
Blood 2013 Sep 5;122(10):1750-60
Blood 2013 Sep 5;122(10):1750-60
Macrophage microRNA-155 promotes cardiac hypertrophy and failure.
Heymans S, Corsten MF, Verhesen W, Carai P, van Leeuwen RE, Custers K, Peters T, Hazebroek M, Stöger L, Wijnands E, Janssen BJ, Creemers EE, Pinto YM, Grimm D, Schürmann N, Vigorito E, Thum T, Stassen F, Yin X, Mayr M, de Windt LJ, Lutgens E, Wouters K, de Winther MP, Zacchigna S, Giacca M, van Bilsen M, Papageorgiou AP, Schroen B
Circulation 2013 Sep 24;128(13):1420-32
Circulation 2013 Sep 24;128(13):1420-32
A component of the mir-17-92 polycistronic oncomir promotes oncogene-dependent apoptosis.
Olive V, Sabio E, Bennett MJ, De Jong CS, Biton A, McGann JC, Greaney SK, Sodir NM, Zhou AY, Balakrishnan A, Foth M, Luftig MA, Goga A, Speed TP, Xuan Z, Evan GI, Wan Y, Minella AC, He L
eLife 2013 Oct 15;2:e00822
eLife 2013 Oct 15;2:e00822
Neuronal and nonneuronal cholinergic structures in the mouse gastrointestinal tract and spleen.
Gautron L, Rutkowski JM, Burton MD, Wei W, Wan Y, Elmquist JK
The Journal of comparative neurology 2013 Nov;521(16):3741-67
The Journal of comparative neurology 2013 Nov;521(16):3741-67
A canonical to non-canonical Wnt signalling switch in haematopoietic stem-cell ageing.
Florian MC, Nattamai KJ, Dörr K, Marka G, Uberle B, Vas V, Eckl C, Andrä I, Schiemann M, Oostendorp RA, Scharffetter-Kochanek K, Kestler HA, Zheng Y, Geiger H
Nature 2013 Nov 21;503(7476):392-6
Nature 2013 Nov 21;503(7476):392-6
MIP-1α/CCL3-mediated maintenance of leukemia-initiating cells in the initiation process of chronic myeloid leukemia.
Baba T, Naka K, Morishita S, Komatsu N, Hirao A, Mukaida N
The Journal of experimental medicine 2013 Nov 18;210(12):2661-73
The Journal of experimental medicine 2013 Nov 18;210(12):2661-73
Natural IgG antibodies provide innate protection against ficolin-opsonized bacteria.
Panda S, Zhang J, Tan NS, Ho B, Ding JL
The EMBO journal 2013 Nov 13;32(22):2905-19
The EMBO journal 2013 Nov 13;32(22):2905-19
Ebf1 and c-Myb repress rag transcription downstream of Stat5 during early B cell development.
Timblin GA, Schlissel MS
Journal of immunology (Baltimore, Md. : 1950) 2013 Nov 1;191(9):4676-87
Journal of immunology (Baltimore, Md. : 1950) 2013 Nov 1;191(9):4676-87
Natural killer cells are required for extramedullary hematopoiesis following murine cytomegalovirus infection.
Jordan S, Ruzsics Z, Mitrović M, Baranek T, Arapović J, Krmpotić A, Vivier E, Dalod M, Jonjić S, Dölken L, Koszinowski UH
Cell host & microbe 2013 May 15;13(5):535-545
Cell host & microbe 2013 May 15;13(5):535-545
Critical role of SAP in progression and reactivation but not maintenance of T cell-dependent humoral immunity.
Zhong MC, Veillette A
Molecular and cellular biology 2013 Mar;33(6):1223-32
Molecular and cellular biology 2013 Mar;33(6):1223-32
SAP modulates B cell functions in a genetic background-dependent manner.
Detre C, Yigit B, Keszei M, Castro W, Magelky EM, Terhorst C
Immunology letters 2013 Jun;153(1-2):15-21
Immunology letters 2013 Jun;153(1-2):15-21
Histone demethylase Lsd1 represses hematopoietic stem and progenitor cell signatures during blood cell maturation.
Kerenyi MA, Shao Z, Hsu YJ, Guo G, Luc S, O'Brien K, Fujiwara Y, Peng C, Nguyen M, Orkin SH
eLife 2013 Jun 18;2:e00633
eLife 2013 Jun 18;2:e00633
RIP1-driven autoinflammation targets IL-1α independently of inflammasomes and RIP3.
Lukens JR, Vogel P, Johnson GR, Kelliher MA, Iwakura Y, Lamkanfi M, Kanneganti TD
Nature 2013 Jun 13;498(7453):224-7
Nature 2013 Jun 13;498(7453):224-7
Reciprocal effects of rab7 deletion in activated and neglected T cells.
Roy SG, Stevens MW, So L, Edinger AL
Autophagy 2013 Jul;9(7):1009-23
Autophagy 2013 Jul;9(7):1009-23
MYBL2 is a sub-haploinsufficient tumor suppressor gene in myeloid malignancy.
Heinrichs S, Conover LF, Bueso-Ramos CE, Kilpivaara O, Stevenson K, Neuberg D, Loh ML, Wu WS, Rodig SJ, Garcia-Manero G, Kantarjian HM, Look AT
eLife 2013 Jul 16;2:e00825
eLife 2013 Jul 16;2:e00825
Avirulent Toxoplasma gondii generates therapeutic antitumor immunity by reversing immunosuppression in the ovarian cancer microenvironment.
Baird JR, Fox BA, Sanders KL, Lizotte PH, Cubillos-Ruiz JR, Scarlett UK, Rutkowski MR, Conejo-Garcia JR, Fiering S, Bzik DJ
Cancer research 2013 Jul 1;73(13):3842-51
Cancer research 2013 Jul 1;73(13):3842-51
Adhesion to substrates induces dendritic cell endothelization and decreases immunological response.
Osterbur J, Sprague L, Muccioli M, Pate M, Mansfield K, McGinty J, Li Y, Li Y, Shirure V, Courreges MC, Benencia F
Immunobiology 2013 Jan;218(1):64-75
Immunobiology 2013 Jan;218(1):64-75
Shared and distinct transcriptional programs underlie the hybrid nature of iNKT cells.
Cohen NR, Brennan PJ, Shay T, Watts GF, Brigl M, Kang J, Brenner MB, ImmGen Project Consortium
Nature immunology 2013 Jan;14(1):90-9
Nature immunology 2013 Jan;14(1):90-9
TiF1-gamma plays an essential role in murine hematopoiesis and regulates transcriptional elongation of erythroid genes.
Bai X, Trowbridge JJ, Riley E, Lee JA, DiBiase A, Kaartinen VM, Orkin SH, Zon LI
Developmental biology 2013 Jan 15;373(2):422-30
Developmental biology 2013 Jan 15;373(2):422-30
The intramembrane protease SPPL2a promotes B cell development and controls endosomal traffic by cleavage of the invariant chain.
Schneppenheim J, Dressel R, Hüttl S, Lüllmann-Rauch R, Engelke M, Dittmann K, Wienands J, Eskelinen EL, Hermans-Borgmeyer I, Fluhrer R, Saftig P, Schröder B
The Journal of experimental medicine 2013 Jan 14;210(1):41-58
The Journal of experimental medicine 2013 Jan 14;210(1):41-58
Cbx4 regulates the proliferation of thymic epithelial cells and thymus function.
Liu B, Liu YF, Du YR, Mardaryev AN, Yang W, Chen H, Xu ZM, Xu CQ, Zhang XR, Botchkarev VA, Zhang Y, Xu GL
Development (Cambridge, England) 2013 Feb;140(4):780-8
Development (Cambridge, England) 2013 Feb;140(4):780-8
T cell-positive selection uses self-ligand binding strength to optimize repertoire recognition of foreign antigens.
Mandl JN, Monteiro JP, Vrisekoop N, Germain RN
Immunity 2013 Feb 21;38(2):263-274
Immunity 2013 Feb 21;38(2):263-274
HDL and Glut1 inhibition reverse a hypermetabolic state in mouse models of myeloproliferative disorders.
Gautier EL, Westerterp M, Bhagwat N, Cremers S, Shih A, Abdel-Wahab O, Lütjohann D, Randolph GJ, Levine RL, Tall AR, Yvan-Charvet L
The Journal of experimental medicine 2013 Feb 11;210(2):339-53
The Journal of experimental medicine 2013 Feb 11;210(2):339-53
The kinase mTOR modulates the antibody response to provide cross-protective immunity to lethal infection with influenza virus.
Keating R, Hertz T, Wehenkel M, Harris TL, Edwards BA, McClaren JL, Brown SA, Surman S, Wilson ZS, Bradley P, Hurwitz J, Chi H, Doherty PC, Thomas PG, McGargill MA
Nature immunology 2013 Dec;14(12):1266-76
Nature immunology 2013 Dec;14(12):1266-76
An intact immune system is required for the anticancer activities of histone deacetylase inhibitors.
West AC, Mattarollo SR, Shortt J, Cluse LA, Christiansen AJ, Smyth MJ, Johnstone RW
Cancer research 2013 Dec 15;73(24):7265-76
Cancer research 2013 Dec 15;73(24):7265-76
Temporal requirements for B cells in the establishment of CD4 T cell memory.
Mollo SB, Zajac AJ, Harrington LE
Journal of immunology (Baltimore, Md. : 1950) 2013 Dec 15;191(12):6052-9
Journal of immunology (Baltimore, Md. : 1950) 2013 Dec 15;191(12):6052-9
Hematopoietic progenitor cell lines with myeloid and lymphoid potential.
Redecke V, Wu R, Zhou J, Finkelstein D, Chaturvedi V, High AA, Häcker H
Nature methods 2013 Aug;10(8):795-803
Nature methods 2013 Aug;10(8):795-803
The β2 integrin-kindlin-3 interaction is essential for T-cell homing but dispensable for T-cell activation in vivo.
Morrison VL, MacPherson M, Savinko T, Lek HS, Prescott A, Fagerholm SC
Blood 2013 Aug 22;122(8):1428-36
Blood 2013 Aug 22;122(8):1428-36
Myeloid-derived suppressor cells play crucial roles in the regulation of mouse collagen-induced arthritis.
Fujii W, Ashihara E, Hirai H, Nagahara H, Kajitani N, Fujioka K, Murakami K, Seno T, Yamamoto A, Ishino H, Kohno M, Maekawa T, Kawahito Y
Journal of immunology (Baltimore, Md. : 1950) 2013 Aug 1;191(3):1073-81
Journal of immunology (Baltimore, Md. : 1950) 2013 Aug 1;191(3):1073-81
Inflammatory neovascularization during graft-versus-host disease is regulated by αv integrin and miR-100.
Leonhardt F, Grundmann S, Behe M, Bluhm F, Dumont RA, Braun F, Fani M, Riesner K, Prinz G, Hechinger AK, Gerlach UV, Dierbach H, Penack O, Schmitt-Gräff A, Finke J, Weber WA, Zeiser R
Blood 2013 Apr 25;121(17):3307-18
Blood 2013 Apr 25;121(17):3307-18
T cell factor 1 is required for group 2 innate lymphoid cell generation.
Yang Q, Monticelli LA, Saenz SA, Chi AW, Sonnenberg GF, Tang J, De Obaldia ME, Bailis W, Bryson JL, Toscano K, Huang J, Haczku A, Pear WS, Artis D, Bhandoola A
Immunity 2013 Apr 18;38(4):694-704
Immunity 2013 Apr 18;38(4):694-704
Extra-thymic physiological T lineage progenitor activity is exclusively confined to cells expressing either CD127, CD90, or high levels of CD117.
Saran N, Pommerencke J, Witzlau K, Regelin M, Krueger A
PloS one 2012;7(2):e30864
PloS one 2012;7(2):e30864
Dominant Role of CD80-CD86 Over CD40 and ICOSL in the Massive Polyclonal B Cell Activation Mediated by LAT(Y136F) CD4(+) T Cells.
Chevrier S, Genton C, Malissen B, Malissen M, Acha-Orbea H
Frontiers in immunology 2012;3:27
Frontiers in immunology 2012;3:27
Incomplete TCR-β allelic exclusion accelerates spontaneous autoimmune arthritis in K/BxN TCR transgenic mice.
Auger JL, Haasken S, Steinert EM, Binstadt BA
European journal of immunology 2012 Sep;42(9):2354-62
European journal of immunology 2012 Sep;42(9):2354-62
Lymphotoxin-beta receptor blockade induces inflammation and fibrosis in tolerized cardiac allografts.
Nakayama Y, Bromberg JS
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2012 Sep;12(9):2322-34
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons 2012 Sep;12(9):2322-34
NK cells are required for costimulatory blockade induced tolerance to vascularized allografts.
van der Touw W, Burrell B, Lal G, Bromberg JS
Transplantation 2012 Sep 27;94(6):575-84
Transplantation 2012 Sep 27;94(6):575-84
Activation-induced cytidine deaminase-initiated off-target DNA breaks are detected and resolved during S phase.
Hasham MG, Snow KJ, Donghia NM, Branca JA, Lessard MD, Stavnezer J, Shopland LS, Mills KD
Journal of immunology (Baltimore, Md. : 1950) 2012 Sep 1;189(5):2374-82
Journal of immunology (Baltimore, Md. : 1950) 2012 Sep 1;189(5):2374-82
PIDDosome-independent tumor suppression by Caspase-2.
Manzl C, Peintner L, Krumschnabel G, Bock F, Labi V, Drach M, Newbold A, Johnstone R, Villunger A
Cell death and differentiation 2012 Oct;19(10):1722-32
Cell death and differentiation 2012 Oct;19(10):1722-32
Molecular definition of the identity and activation of natural killer cells.
Bezman NA, Kim CC, Sun JC, Min-Oo G, Hendricks DW, Kamimura Y, Best JA, Goldrath AW, Lanier LL, Immunological Genome Project Consortium
Nature immunology 2012 Oct;13(10):1000-9
Nature immunology 2012 Oct;13(10):1000-9
Kruppel-like factor 7 overexpression suppresses hematopoietic stem and progenitor cell function.
Schuettpelz LG, Gopalan PK, Giuste FO, Romine MP, van Os R, Link DC
Blood 2012 Oct 11;120(15):2981-9
Blood 2012 Oct 11;120(15):2981-9
Peroxisome proliferator-activated receptor γ B cell-specific-deficient mice have an impaired antibody response.
Ramon S, Bancos S, Thatcher TH, Murant TI, Moshkani S, Sahler JM, Bottaro A, Sime PJ, Phipps RP
Journal of immunology (Baltimore, Md. : 1950) 2012 Nov 15;189(10):4740-7
Journal of immunology (Baltimore, Md. : 1950) 2012 Nov 15;189(10):4740-7
A role for GPx3 in activity of normal and leukemia stem cells.
Herault O, Hope KJ, Deneault E, Mayotte N, Chagraoui J, Wilhelm BT, Cellot S, Sauvageau M, Andrade-Navarro MA, Hébert J, Sauvageau G
The Journal of experimental medicine 2012 May 7;209(5):895-901
The Journal of experimental medicine 2012 May 7;209(5):895-901
Dll4-Notch signaling in Flt3-independent dendritic cell development and autoimmunity in mice.
Billiard F, Lobry C, Darrasse-Jèze G, Waite J, Liu X, Mouquet H, DaNave A, Tait M, Idoyaga J, Leboeuf M, Kyratsous CA, Burton J, Kalter J, Klinakis A, Zhang W, Thurston G, Merad M, Steinman RM, Murphy AJ, Yancopoulos GD, Aifantis I, Skokos D
The Journal of experimental medicine 2012 May 7;209(5):1011-28
The Journal of experimental medicine 2012 May 7;209(5):1011-28
CD70-driven costimulation induces survival or Fas-mediated apoptosis of T cells depending on antigenic load.
Wensveen FM, Unger PP, Kragten NA, Derks IA, ten Brinke A, Arens R, van Lier RA, Eldering E, van Gisbergen KP
Journal of immunology (Baltimore, Md. : 1950) 2012 May 1;188(9):4256-67
Journal of immunology (Baltimore, Md. : 1950) 2012 May 1;188(9):4256-67
Exposure to nicotine adversely affects the dendritic cell system and compromises host response to vaccination.
Nouri-Shirazi M, Guinet E
Journal of immunology (Baltimore, Md. : 1950) 2012 Mar 1;188(5):2359-70
Journal of immunology (Baltimore, Md. : 1950) 2012 Mar 1;188(5):2359-70
Zbtb46 expression distinguishes classical dendritic cells and their committed progenitors from other immune lineages.
Satpathy AT, KC W, Albring JC, Edelson BT, Kretzer NM, Bhattacharya D, Murphy TL, Murphy KM
The Journal of experimental medicine 2012 Jun 4;209(6):1135-52
The Journal of experimental medicine 2012 Jun 4;209(6):1135-52
Fibroblast growth factor-7 partially reverses murine thymocyte progenitor aging by repression of Ink4a.
Berent-Maoz B, Montecino-Rodriguez E, Signer RA, Dorshkind K
Blood 2012 Jun 14;119(24):5715-21
Blood 2012 Jun 14;119(24):5715-21
Lung B cells promote early pathogen dissemination and hasten death from inhalation anthrax.
Rayamajhi M, Delgado C, Condon TV, Riches DW, Lenz LL
Mucosal immunology 2012 Jul;5(4):444-54
Mucosal immunology 2012 Jul;5(4):444-54
Identification of a genetic locus controlling bacteria-driven colitis and associated cancer through effects on innate inflammation.
Boulard O, Kirchberger S, Royston DJ, Maloy KJ, Powrie FM
The Journal of experimental medicine 2012 Jul 2;209(7):1309-24
The Journal of experimental medicine 2012 Jul 2;209(7):1309-24
IL-21 promotes lupus-like disease in chronic graft-versus-host disease through both CD4 T cell- and B cell-intrinsic mechanisms.
Nguyen V, Luzina I, Rus H, Tegla C, Chen C, Rus V
Journal of immunology (Baltimore, Md. : 1950) 2012 Jul 15;189(2):1081-93
Journal of immunology (Baltimore, Md. : 1950) 2012 Jul 15;189(2):1081-93
Role of mast cells and basophils in IgE responses and in allergic airway hyperresponsiveness.
Sawaguchi M, Tanaka S, Nakatani Y, Harada Y, Mukai K, Matsunaga Y, Ishiwata K, Oboki K, Kambayashi T, Watanabe N, Karasuyama H, Nakae S, Inoue H, Kubo M
Journal of immunology (Baltimore, Md. : 1950) 2012 Feb 15;188(4):1809-18
Journal of immunology (Baltimore, Md. : 1950) 2012 Feb 15;188(4):1809-18
Critical role of B cell lymphoma 10 in BAFF-regulated NF-κB activation and survival of anergic B cells.
Yu M, Chen Y, He Y, Podd A, Fu G, Wright JA, Kleiman E, Khan WN, Wen R, Wang D
Journal of immunology (Baltimore, Md. : 1950) 2012 Dec 1;189(11):5185-93
Journal of immunology (Baltimore, Md. : 1950) 2012 Dec 1;189(11):5185-93
MicroRNA profiling identifies microRNA-155 as an adverse mediator of cardiac injury and dysfunction during acute viral myocarditis.
Corsten MF, Papageorgiou A, Verhesen W, Carai P, Lindow M, Obad S, Summer G, Coort SL, Hazebroek M, van Leeuwen R, Gijbels MJ, Wijnands E, Biessen EA, De Winther MP, Stassen FR, Carmeliet P, Kauppinen S, Schroen B, Heymans S
Circulation research 2012 Aug 3;111(4):415-25
Circulation research 2012 Aug 3;111(4):415-25
PAD4-mediated neutrophil extracellular trap formation is not required for immunity against influenza infection.
Hemmers S, Teijaro JR, Arandjelovic S, Mowen KA
PloS one 2011;6(7):e22043
PloS one 2011;6(7):e22043
FTY720 reduces post-ischemic brain lymphocyte influx but does not improve outcome in permanent murine cerebral ischemia.
Liesz A, Sun L, Zhou W, Schwarting S, Mracsko E, Zorn M, Bauer H, Sommer C, Veltkamp R
PloS one 2011;6(6):e21312
PloS one 2011;6(6):e21312
Genetics and the environment converge to dysregulate N-glycosylation in multiple sclerosis.
Mkhikian H, Grigorian A, Li CF, Chen HL, Newton B, Zhou RW, Beeton C, Torossian S, Tatarian GG, Lee SU, Lau K, Walker E, Siminovitch KA, Chandy KG, Yu Z, Dennis JW, Demetriou M
Nature communications 2011;2:334
Nature communications 2011;2:334
The inflammasome adaptor ASC regulates the function of adaptive immune cells by controlling Dock2-mediated Rac activation and actin polymerization.
Ippagunta SK, Malireddi RK, Shaw PJ, Neale GA, Vande Walle L, Green DR, Fukui Y, Lamkanfi M, Kanneganti TD
Nature immunology 2011 Sep 4;12(10):1010-6
Nature immunology 2011 Sep 4;12(10):1010-6
NKT cells are required for complete Freund's adjuvant-mediated protection from autoimmune diabetes.
Lee IF, van den Elzen P, Tan R, Priatel JJ
Journal of immunology (Baltimore, Md. : 1950) 2011 Sep 15;187(6):2898-904
Journal of immunology (Baltimore, Md. : 1950) 2011 Sep 15;187(6):2898-904
Anti-CD180 (RP105) activates B cells to rapidly produce polyclonal Ig via a T cell and MyD88-independent pathway.
Chaplin JW, Kasahara S, Clark EA, Ledbetter JA
Journal of immunology (Baltimore, Md. : 1950) 2011 Oct 15;187(8):4199-209
Journal of immunology (Baltimore, Md. : 1950) 2011 Oct 15;187(8):4199-209
Chemokines control naive CD8+ T cell selection of optimal lymph node antigen presenting cells.
Hickman HD, Li L, Reynoso GV, Rubin EJ, Skon CN, Mays JW, Gibbs J, Schwartz O, Bennink JR, Yewdell JW
The Journal of experimental medicine 2011 Nov 21;208(12):2511-24
The Journal of experimental medicine 2011 Nov 21;208(12):2511-24
Identification of semaphorin 4B as a negative regulator of basophil-mediated immune responses.
Nakagawa Y, Takamatsu H, Okuno T, Kang S, Nojima S, Kimura T, Kataoka TR, Ikawa M, Toyofuku T, Katayama I, Kumanogoh A
Journal of immunology (Baltimore, Md. : 1950) 2011 Mar 1;186(5):2881-8
Journal of immunology (Baltimore, Md. : 1950) 2011 Mar 1;186(5):2881-8
L2pB1: a new player in autoimmunity.
Zhong X, Rothstein TL
Molecular immunology 2011 Jun;48(11):1292-300
Molecular immunology 2011 Jun;48(11):1292-300
Recent thymic emigrants are biased against the T-helper type 1 and toward the T-helper type 2 effector lineage.
Hendricks DW, Fink PJ
Blood 2011 Jan 27;117(4):1239-49
Blood 2011 Jan 27;117(4):1239-49
RNA-seq analysis of 2 closely related leukemia clones that differ in their self-renewal capacity.
Wilhelm BT, Briau M, Austin P, Faubert A, Boucher G, Chagnon P, Hope K, Girard S, Mayotte N, Landry JR, Hébert J, Sauvageau G
Blood 2011 Jan 13;117(2):e27-38
Blood 2011 Jan 13;117(2):e27-38
Nuclear export of the NF-κB inhibitor IκBα is required for proper B cell and secondary lymphoid tissue formation.
Wuerzberger-Davis SM, Chen Y, Yang DT, Kearns JD, Bates PW, Lynch C, Ladell NC, Yu M, Podd A, Zeng H, Huang TT, Wen R, Hoffmann A, Wang D, Miyamoto S
Immunity 2011 Feb 25;34(2):188-200
Immunity 2011 Feb 25;34(2):188-200
A subset of interleukin-21+ chemokine receptor CCR9+ T helper cells target accessory organs of the digestive system in autoimmunity.
McGuire HM, Vogelzang A, Ma CS, Hughes WE, Silveira PA, Tangye SG, Christ D, Fulcher D, Falcone M, King C
Immunity 2011 Apr 22;34(4):602-15
Immunity 2011 Apr 22;34(4):602-15
T-bet and eomesodermin are required for T cell-mediated antitumor immune responses.
Zhu Y, Ju S, Chen E, Dai S, Li C, Morel P, Liu L, Zhang X, Lu B
Journal of immunology (Baltimore, Md. : 1950) 2010 Sep 15;185(6):3174-83
Journal of immunology (Baltimore, Md. : 1950) 2010 Sep 15;185(6):3174-83
Glycosidic Tn-based vaccines targeting dermal dendritic cells favor germinal center B-cell development and potent antibody response in the absence of adjuvant.
Freire T, Zhang X, Dériaud E, Ganneau C, Vichier-Guerre S, Azria E, Launay O, Lo-Man R, Bay S, Leclerc C
Blood 2010 Nov 4;116(18):3526-36
Blood 2010 Nov 4;116(18):3526-36
Store-operated Ca2+ entry through ORAI1 is critical for T cell-mediated autoimmunity and allograft rejection.
McCarl CA, Khalil S, Ma J, Oh-hora M, Yamashita M, Roether J, Kawasaki T, Jairaman A, Sasaki Y, Prakriya M, Feske S
Journal of immunology (Baltimore, Md. : 1950) 2010 Nov 15;185(10):5845-58
Journal of immunology (Baltimore, Md. : 1950) 2010 Nov 15;185(10):5845-58
Notch dimerization is required for leukemogenesis and T-cell development.
Liu H, Chi AW, Arnett KL, Chiang MY, Xu L, Shestova O, Wang H, Li YM, Bhandoola A, Aster JC, Blacklow SC, Pear WS
Genes & development 2010 Nov 1;24(21):2395-407
Genes & development 2010 Nov 1;24(21):2395-407
Steroid-resistant lymphatic remodeling in chronically inflamed mouse airways.
Yao LC, Baluk P, Feng J, McDonald DM
The American journal of pathology 2010 Mar;176(3):1525-41
The American journal of pathology 2010 Mar;176(3):1525-41
Dendritic cell (DC)-specific targeting reveals Stat3 as a negative regulator of DC function.
Melillo JA, Song L, Bhagat G, Blazquez AB, Plumlee CR, Lee C, Berin C, Reizis B, Schindler C
Journal of immunology (Baltimore, Md. : 1950) 2010 Mar 1;184(5):2638-45
Journal of immunology (Baltimore, Md. : 1950) 2010 Mar 1;184(5):2638-45
Separation of graft-versus-host disease from graft-versus-leukemia responses by targeting CC-chemokine receptor 7 on donor T cells.
Coghill JM, Carlson MJ, Panoskaltsis-Mortari A, West ML, Burgents JE, Blazar BR, Serody JS
Blood 2010 Jun 10;115(23):4914-22
Blood 2010 Jun 10;115(23):4914-22
Mad3 negatively regulates B cell differentiation in the spleen by inducing Id2 expression.
Gore Y, Lantner F, Hart G, Shachar I
Molecular biology of the cell 2010 Jun 1;21(11):1864-71
Molecular biology of the cell 2010 Jun 1;21(11):1864-71
Intermediate-term hematopoietic stem cells with extended but time-limited reconstitution potential.
Benveniste P, Frelin C, Janmohamed S, Barbara M, Herrington R, Hyam D, Iscove NN
Cell stem cell 2010 Jan 8;6(1):48-58
Cell stem cell 2010 Jan 8;6(1):48-58
AUF1 is involved in splenic follicular B cell maintenance.
Sadri N, Lu JY, Badura ML, Schneider RJ
BMC immunology 2010 Jan 11;11:1
BMC immunology 2010 Jan 11;11:1
Suppression of B-cell lymphomagenesis by the BH3-only proteins Bmf and Bad.
Frenzel A, Labi V, Chmelewskij W, Ploner C, Geley S, Fiegl H, Tzankov A, Villunger A
Blood 2010 Feb 4;115(5):995-1005
Blood 2010 Feb 4;115(5):995-1005
The PI3K isoforms p110alpha and p110delta are essential for pre-B cell receptor signaling and B cell development.
Ramadani F, Bolland DJ, Garcon F, Emery JL, Vanhaesebroeck B, Corcoran AE, Okkenhaug K
Science signaling 2010 Aug 10;3(134):ra60
Science signaling 2010 Aug 10;3(134):ra60
A novel heat-shock protein coinducer boosts stress protein Hsp70 to activate T cell regulation of inflammation in autoimmune arthritis.
Wieten L, van der Zee R, Spiering R, Wagenaar-Hilbers J, van Kooten P, Broere F, van Eden W
Arthritis and rheumatism 2010 Apr;62(4):1026-35
Arthritis and rheumatism 2010 Apr;62(4):1026-35
Toll-like receptor 3-mediated suppression of TRAMP prostate cancer shows the critical role of type I interferons in tumor immune surveillance.
Chin AI, Miyahira AK, Covarrubias A, Teague J, Guo B, Dempsey PW, Cheng G
Cancer research 2010 Apr 1;70(7):2595-603
Cancer research 2010 Apr 1;70(7):2595-603
Gene expression profiling of leukemia stem cells.
Krivtsov AV, Wang Y, Feng Z, Armstrong SA
Methods in molecular biology (Clifton, N.J.) 2009;538:231-46
Methods in molecular biology (Clifton, N.J.) 2009;538:231-46
PI3 kinase signals BCR-dependent mature B cell survival.
Srinivasan L, Sasaki Y, Calado DP, Zhang B, Paik JH, DePinho RA, Kutok JL, Kearney JF, Otipoby KL, Rajewsky K
Cell 2009 Oct 30;139(3):573-86
Cell 2009 Oct 30;139(3):573-86
Id1 represses osteoclast-dependent transcription and affects bone formation and hematopoiesis.
Chan AS, Jensen KK, Skokos D, Doty S, Lederman HK, Kaplan RN, Rafii S, Rivella S, Lyden D
PloS one 2009 Nov 24;4(11):e7955
PloS one 2009 Nov 24;4(11):e7955
Chemokine-like receptor-1 expression by central nervous system-infiltrating leukocytes and involvement in a model of autoimmune demyelinating disease.
Graham KL, Zabel BA, Loghavi S, Zuniga LA, Ho PP, Sobel RA, Butcher EC
Journal of immunology (Baltimore, Md. : 1950) 2009 Nov 15;183(10):6717-23
Journal of immunology (Baltimore, Md. : 1950) 2009 Nov 15;183(10):6717-23
Chronic CD70-driven costimulation impairs IgG responses by instructing T cells to inhibit germinal center B cell formation through FasL-Fas interactions.
Beishuizen CR, Kragten NA, Boon L, Nolte MA, van Lier RA, van Gisbergen KP
Journal of immunology (Baltimore, Md. : 1950) 2009 Nov 15;183(10):6442-51
Journal of immunology (Baltimore, Md. : 1950) 2009 Nov 15;183(10):6442-51
A critical role of TAK1 in B-cell receptor-mediated nuclear factor kappaB activation.
Schuman J, Chen Y, Podd A, Yu M, Liu HH, Wen R, Chen ZJ, Wang D
Blood 2009 May 7;113(19):4566-74
Blood 2009 May 7;113(19):4566-74
Dynamic imaging of T cell-parasite interactions in the brains of mice chronically infected with Toxoplasma gondii.
Schaeffer M, Han SJ, Chtanova T, van Dooren GG, Herzmark P, Chen Y, Roysam B, Striepen B, Robey EA
Journal of immunology (Baltimore, Md. : 1950) 2009 May 15;182(10):6379-93
Journal of immunology (Baltimore, Md. : 1950) 2009 May 15;182(10):6379-93
Critical roles of lysosomal acid lipase in T cell development and function.
Qu P, Du H, Wilkes DS, Yan C
The American journal of pathology 2009 Mar;174(3):944-56
The American journal of pathology 2009 Mar;174(3):944-56
Oncogenic Kras initiates leukemia in hematopoietic stem cells.
Sabnis AJ, Cheung LS, Dail M, Kang HC, Santaguida M, Hermiston ML, Passegué E, Shannon K, Braun BS
PLoS biology 2009 Mar 17;7(3):e59
PLoS biology 2009 Mar 17;7(3):e59
CD93 is required for maintenance of antibody secretion and persistence of plasma cells in the bone marrow niche.
Chevrier S, Genton C, Kallies A, Karnowski A, Otten LA, Malissen B, Malissen M, Botto M, Corcoran LM, Nutt SL, Acha-Orbea H
Proceedings of the National Academy of Sciences of the United States of America 2009 Mar 10;106(10):3895-900
Proceedings of the National Academy of Sciences of the United States of America 2009 Mar 10;106(10):3895-900
AID constrains germinal center size by rendering B cells susceptible to apoptosis.
Zaheen A, Boulianne B, Parsa JY, Ramachandran S, Gommerman JL, Martin A
Blood 2009 Jul 16;114(3):547-54
Blood 2009 Jul 16;114(3):547-54
Apoptotic cells promote their own clearance and immune tolerance through activation of the nuclear receptor LXR.
A-Gonzalez N, Bensinger SJ, Hong C, Beceiro S, Bradley MN, Zelcer N, Deniz J, Ramirez C, Díaz M, Gallardo G, de Galarreta CR, Salazar J, Lopez F, Edwards P, Parks J, Andujar M, Tontonoz P, Castrillo A
Immunity 2009 Aug 21;31(2):245-58
Immunity 2009 Aug 21;31(2):245-58
Migratory and antigen presentation functions of IFN-producing killer dendritic cells.
Himoudi N, Yan M, Bouma G, Morgenstern D, Wallace R, Seddon B, Buddle J, Eddaoudi A, Howe SJ, Cooper N, Anderson J
Cancer research 2009 Aug 15;69(16):6598-606
Cancer research 2009 Aug 15;69(16):6598-606
Peripheral dendritic cells are essential for both the innate and adaptive antiviral immune responses in the central nervous system.
Steel CD, Hahto SM, Ciavarra RP
Virology 2009 Apr 25;387(1):117-26
Virology 2009 Apr 25;387(1):117-26
Fundamental differences in the dynamics of CNS lesion development and composition in MP4- and MOG peptide 35-55-induced experimental autoimmune encephalomyelitis.
Kuerten S, Javeri S, Tary-Lehmann M, Lehmann PV, Angelov DN
Clinical immunology (Orlando, Fla.) 2008 Nov;129(2):256-67
Clinical immunology (Orlando, Fla.) 2008 Nov;129(2):256-67
Age-dependent tolerance to an endogenous tumor-associated antigen.
McWilliams JA, Sullivan RT, Jordan KR, McMahan RH, Kemmler CB, McDuffie M, Slansky JE
Vaccine 2008 Mar 28;26(15):1863-1873
Vaccine 2008 Mar 28;26(15):1863-1873
The receptor tyrosine kinase Flt3 is required for dendritic cell development in peripheral lymphoid tissues.
Waskow C, Liu K, Darrasse-Jèze G, Guermonprez P, Ginhoux F, Merad M, Shengelia T, Yao K, Nussenzweig M
Nature immunology 2008 Jun;9(6):676-83
Nature immunology 2008 Jun;9(6):676-83
Donor major histocompatibility complex class I expression determines the outcome of prenatal transplantation.
Durkin ET, Jones KA, Elnaggar D, Shaaban AF
Journal of pediatric surgery 2008 Jun;43(6):1142-7
Journal of pediatric surgery 2008 Jun;43(6):1142-7
Regulatory role of B cells in a murine model of allergic airway disease.
Singh A, Carson WF 4th, Secor ER Jr, Guernsey LA, Flavell RA, Clark RB, Thrall RS, Schramm CM
Journal of immunology (Baltimore, Md. : 1950) 2008 Jun 1;180(11):7318-26
Journal of immunology (Baltimore, Md. : 1950) 2008 Jun 1;180(11):7318-26
Regulatory role of B cells in a murine model of allergic airway disease.
Singh A, Carson WF 4th, Secor ER Jr, Guernsey LA, Flavell RA, Clark RB, Thrall RS, Schramm CM
Journal of immunology (Baltimore, Md. : 1950) 2008 Jun 1;180(11):7318-26
Journal of immunology (Baltimore, Md. : 1950) 2008 Jun 1;180(11):7318-26
Activation-induced cytidine deaminase deficiency causes organ-specific autoimmune disease.
Hase K, Takahashi D, Ebisawa M, Kawano S, Itoh K, Ohno H
PloS one 2008 Aug 21;3(8):e3033
PloS one 2008 Aug 21;3(8):e3033
p27 deficiency cooperates with Bcl-2 but not Bax to promote T-cell lymphoma.
Cheng N, van de Wetering CI, Knudson CM
PloS one 2008 Apr 2;3(4):e1911
PloS one 2008 Apr 2;3(4):e1911
Phospholipase Cgamma2 contributes to light-chain gene activation and receptor editing.
Bai L, Chen Y, He Y, Dai X, Lin X, Wen R, Wang D
Molecular and cellular biology 2007 Sep;27(17):5957-67
Molecular and cellular biology 2007 Sep;27(17):5957-67
N-glycan processing deficiency promotes spontaneous inflammatory demyelination and neurodegeneration.
Lee SU, Grigorian A, Pawling J, Chen IJ, Gao G, Mozaffar T, McKerlie C, Demetriou M
The Journal of biological chemistry 2007 Nov 16;282(46):33725-33734
The Journal of biological chemistry 2007 Nov 16;282(46):33725-33734
Selective depletion of Foxp3+ regulatory T cells induces a scurfy-like disease.
Lahl K, Loddenkemper C, Drouin C, Freyer J, Arnason J, Eberl G, Hamann A, Wagner H, Huehn J, Sparwasser T
The Journal of experimental medicine 2007 Jan 22;204(1):57-63
The Journal of experimental medicine 2007 Jan 22;204(1):57-63
Murine CXCL14 is dispensable for dendritic cell function and localization within peripheral tissues.
Meuter S, Schaerli P, Roos RS, Brandau O, Bösl MR, von Andrian UH, Moser B
Molecular and cellular biology 2007 Feb;27(3):983-92
Molecular and cellular biology 2007 Feb;27(3):983-92
Small intestine lamina propria dendritic cells promote de novo generation of Foxp3 T reg cells via retinoic acid.
Sun CM, Hall JA, Blank RB, Bouladoux N, Oukka M, Mora JR, Belkaid Y
The Journal of experimental medicine 2007 Aug 6;204(8):1775-85
The Journal of experimental medicine 2007 Aug 6;204(8):1775-85
Identification of a radio-resistant and cycling dermal dendritic cell population in mice and men.
Bogunovic M, Ginhoux F, Wagers A, Loubeau M, Isola LM, Lubrano L, Najfeld V, Phelps RG, Grosskreutz C, Scigliano E, Frenette PS, Merad M
The Journal of experimental medicine 2006 Nov 27;203(12):2627-38
The Journal of experimental medicine 2006 Nov 27;203(12):2627-38
Essential role of phospholipase C gamma 2 in early B-cell development and Myc-mediated lymphomagenesis.
Wen R, Chen Y, Bai L, Fu G, Schuman J, Dai X, Zeng H, Yang C, Stephan RP, Cleveland JL, Wang D
Molecular and cellular biology 2006 Dec;26(24):9364-76
Molecular and cellular biology 2006 Dec;26(24):9364-76
A role for brain-derived neurotrophic factor in B cell development.
Schuhmann B, Dietrich A, Sel S, Hahn C, Klingenspor M, Lommatzsch M, Gudermann T, Braun A, Renz H, Nockher WA
Journal of neuroimmunology 2005 Jun;163(1-2):15-23
Journal of neuroimmunology 2005 Jun;163(1-2):15-23
Impaired immune responses and B-cell proliferation in mice lacking the Id3 gene.
Pan L, Sato S, Frederick JP, Sun XH, Zhuang Y
Molecular and cellular biology 1999 Sep;19(9):5969-80
Molecular and cellular biology 1999 Sep;19(9):5969-80
Identification of monoclonal antibodies for immunohistochemical staining of feline B lymphocytes in frozen and formalin-fixed paraffin-embedded tissues.
Monteith CE, Chelack BJ, Davis WC, Haines DM
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire 1996 Jul;60(3):193-8
Canadian journal of veterinary research = Revue canadienne de recherche veterinaire 1996 Jul;60(3):193-8
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- Immunohistochemical analysis of CD45R (B220) was performed using formalin-fixed paraffin-embedded human tonsil tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using using eBioscience™ IHC Antigen Retrieval Solution - Low pH (10X) (Product # 00-4955-58) diluted to 1X solution in water in a microwave at 100 degree Celsius for 10 minutes. Following antigen retrieval, the sections were blocked with 2% normal goat serum in 1X PBS for 45 minutes at room temperature and then probed with or without CD45R (B220) Monoclonal Antibody (RA3-6B2), eBioscience™ (Product # 14-0452-82) at 5 µg/mL concentration in 0.1% normal goat serum overnight at 4 degree Celsius in a humidified chamber. Detection was performed using Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (Product # A-11006) at a dilution of 1:2000 in 0.1% normal goat serum for 45 minutes at room temperature. ReadyProbes™ Tissue Autofluorescence Quenching Kit (Product # R37630) was used to quench autofluorescence from the tissues. Nuclei were stained with DAPI (Product # D1306) and the sections were mounted using ProLong™ Glass Antifade Mountant (Product # P36984). The images were captured on EVOS™ M7000 Imaging System (Product # AMF7000) at 20X magnification and deconvoluted externally.
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- Immunohistochemical analysis of CD45R (B220) was performed using formalin-fixed paraffin-embedded human tonsil tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using using eBioscience™ IHC Antigen Retrieval Solution - Low pH (10X) (Product # 00-4955-58) diluted to 1X solution in water in a microwave at 100 degree Celsius for 10 minutes. Following antigen retrieval, the sections were blocked with 2% normal goat serum in 1X PBS for 45 minutes at room temperature and then probed with or without CD45R (B220) Monoclonal Antibody (RA3-6B2), eBioscience™ (Product # 14-0452-82) at 5 µg/mL concentration in 0.1% normal goat serum overnight at 4 degree Celsius in a humidified chamber. Detection was performed using Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488 (Product # A-11006) at a dilution of 1:2000 in 0.1% normal goat serum for 45 minutes at room temperature. ReadyProbes™ Tissue Autofluorescence Quenching Kit (Product # R37630) was used to quench autofluorescence from the tissues. Nuclei were stained with DAPI (Product # D1306) and the sections were mounted using ProLong™ Glass Antifade Mountant (Product # P36984). The images were captured on EVOS™ M7000 Imaging System (Product # AMF7000) at 20X magnification and deconvoluted externally.
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- Immunohistochemistry of formalin-fixed paraffin embedded mouse spleen using 5 µg/mL Anti-Human/Mouse CD45R (B220) Purified followed by Anti-Rat IgG Biotin, Avidin HRP, and DAB visualization. Nuclei are counterstained with hematoxylin.
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- Staining of C57Bl/6 splenocytes with 0.5 µg of Rat IgG2a Isotype Control Purified (Product # 14-4321-82) (open histogram) or 0.5 µg of Anti-Human/Mouse CD45R (B220) Purified (filled histogram) followed by Anti-Rat IgG FITC (Product # 11-4811-85). Cells in the lymphocyte gate were used for analysis.
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- Figure 4 Double immunofluorescence localisation of B220 (Green) and NF-kappaB p65 (Red) in HCV-Tg mice and the fractionation analysis of mouse tissues. A : Co-localisation of NF-kappaB p65 immunoreactivity with B220 is indicated by arrows. (a-b) Cells double-positive for B220 and NF-kappaB in the control mouse (CD19cre). (c-d) Cells double-positive for B220 and NF-kappaB in the asymptomatic HCV-Tg mouse (RzCD19cre). (e-f) Cells double-positive for B220 and NF-kappaB in the lymphomatous HCV-Tg mouse (RzCD19cre). B : Quantitative analysis of the ratio of double-positive cells among B220-positive cells in each HCV-Tg mouse. Bar graph indicates the percentage of cells with NF-kappaB p65 nuclear translocation in B220-positive cells. C : Bar graph shows the ratio of double-positive cells within the B220-positive cells in normal, asymptomatic and lymphomatous HCV-Tg mice. Ho: Hoechst33342 Data are presented as means +- S.E., * P
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- Figure 7 Marginal zone B cell regions were narrowed in Ziz2 KO mice. (A) Spleen sections were stained with anti-B220 (Red) and anti-CD169 (Green) antibodies for the MZ B cell region (B220-positive region outside CD169-positive cells). All mice were 10 weeks old. Scale bars: 100 mum. (B) MZ B cell regions were narrower in Ziz2 and Ziz3 KO mice than in wild type mice. (C) The proliferative activity of MZ B cells in response to LPS was not altered in both KO mice. Three mice per group were used. Data from three independent experiments (one mouse per group per experiment was used) were summarized. (D) The migratory activity of MZ B cells was analyzed using a transwell and flow cytometry. Activity against BLC or SDF1 (SDF) was not altered in both KO mice. Four mice per group were used. Each plot indicates data from one mouse. Data from four independent experiments (one mouse per group per experiment was used) were summarized. 2KO: Ziz2 KO. 3KO: Ziz3 KO. ***: P < 0.0001.
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- Figure 3 GPR183 and 7alpha,25-OHC Promote ILC3 Localization to CPs and ILFs (A) Distribution of Gpr183 +/+ and Gpr183 -deficient hematopoietic cells in the colon of mixed bone marrow chimeras. Bone marrow cells from Gpr183 +/+ or Gpr183 -/- mice (CD45.2 + ) were mixed 9:1 with B6 cells (CD45.1 + ) and injected into irradiated Rag1 -/- recipients (CD45.1 + ) for the generation of bone marrow chimeras. Sections were stained for detection of Gpr183 +/+ and Gpr183 -/- cells (CD45.2, red) or B6 cells (CD45.1, green). Nuclei were visualized by DAPI staining (blue). Scale bars on the right (white) represent 100 mum. (B) Distribution of Gpr183 +/+ and Gpr183 -deficient ILCs in the colon of mixed bone marrow chimeras. Bone marrow cells from Gpr183 +/+ or Gpr183 -/- mice (CD90.2 + ) were mixed 9:1 with B6 cells (CD90.1 + ) and injected into irradiated B6 recipients (CD90.1 + ) for the generation of bone marrow chimeras. Colon sections were stained for detection of Gpr183 +/+ and Gpr183 -/- (CD90.2, red) or B6 (CD90.1, green) ILCs. Scale bars (red) represent 100 mum. The lower panel shows the number of clusters in Gpr183 +/+ -B6 and Gpr183 -/- -B6 chimeras consisting of CD90.2 + (red) or CD90.1 + (green) ILCs. Data are represented as means +- SEM. p value by two-way ANOVA. (C and D) Distribution of donor-derived ILC3s (RORgammat-GFP + , green) in the colon (C) and small intestine (D) of bone marrow chimeras. Bone marrow cells from Rag1 -deficient Rorc(gamma
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- Figure 3. Localization of intestinal IgD + B Cells. (A) Representative photomicrographs of small intestinal sections stained with DAPI- and fluorophore-conjugated Ab for IgD (FITC), IgA (PE), and B220 (APC) as indicated from GF ( n = 4) and conventionalized SW littermates (Col, n = 4). Photomicrographs shown are representative of one of two independent experiments. (B) Dot plot showing the number of LP IgA + cells and IgD + B220 + cells per high power field (200x) per GF ( n = 4; 10 fields/mouse) and conventionalized SW littermates (Col; n = 4; 11 fields/mouse) described as in A. (C) Dot plots showing the perpendicular distance (see Materials and methods) between individual LP IgA + cells ( n = 85 for GF; n = 971 for Col), IgD + B220 + cells ( n = 59 for GF; n = 108 for Col), and the serosal surface from GF ( n = 4) and colonized SW littermates (Col, n = 4) described as in A. (D) Representative photomicrographs of small intestinal sections stained as in A from SPF SW mice at the age of postnatal day 21 ( n = 3), 42 ( n = 3), and 120 ( n = 3). Negative control uMT mice ( n = 2) are also shown. White arrows indicate IgD + B220 + cells. Bars, 60 um. Photomicrographs are representative images from one of three independent experiments for each time point and two independent experiments for uMT mice. (E) Dot plot showing the number of IgA + cells and IgD + B220 + cells per high power field (200x) from SPF SW mice and uMT mice described as
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- Fig. 1 Gdpd3 is implicated in CML disease initiation in vivo. a Diagram of pathways of lysophospholipid biosynthesis. G3P is converted into LPAs, and LPAs are then converted into phospholipids by the addition of polar bases via the Kennedy (de novo) pathway. The Lands'' cycle (remodelling pathway) generates lysophospholipids of distinct composition by substituting fatty acid ester and polar base groups of phospholipids. Lysophospholipase D Gdpd3 converts lysophospholipids back into LPAs by catalysing hydrolysis (magenta dotted line). (PC Phosphatidylcholine, PS Phosphatidylserine, PE Phosphatidylethanolamine, PI Phosphatidylinositol, LPC Lysophosphatidylcholine, LPS Lysophosphatidylserine, LPE Lysophosphatidylethanolamine, LPI Lysophosphatidylinositol). b qRT-PCR determination of Gdpd3 mRNA expression in LT-stem (LT), CD48, MPP, and LK cells (see Supplementary Fig.2) isolated from Gdpd3 +/+ tet-CML-affected (SCL-tTA + TRE-BCR-ABL1 + ) mice (one male, six females) or normal littermate (SCL-tTA + ) mice (four males, four females). Data are the mean ratio +- s.d. of transcript levels normalised to Actb ( n = 3 biologically independent samples) ( P -value, unpaired two-sided Student''s t -test). c Quantitation of the colony-forming capacity of Gdpd3 +/+ CML-LSK cells that were transduced with/without Cy3-labelled siRNA targetting mouse Gdpd3 mRNA (mGdpd3 siRNA #1 or #3). Cy3 + and Cy3 - CML-LSK cells were purified at 3 days post-transduction and plated in semi-solid methylcellulo
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- Fig. 7 Lgr4/Gpr48 is involved in CML stem cell self-renewal in vivo. a RNA-Seq determinations of mRNA levels of the indicated GPCR family genes in LT-CML stem cells isolated from Gdpd3 +/+ tet-CML-affected mice (22 males, 12 females) and Gdpd3 -/- tet-CML-affected mice (five males, five females). Results are expressed as FPKM (see Methods). Data are the mean FPKM +- s.d. ( n = 3 biologically independent samples) ( P -value, unpaired two-sided Student''s t -test). Results of the MA-plot and GO term enrichment analyses for these RNA-Seq data are shown in Supplementary Fig. 11a, b . b Quantitation of the colony-forming capacity of Gdpd3 +/+ CML-LSK cells that were transduced with/without Cy3-labelled siRNA targetting mouse Lgr4/Gpr48 mRNA (mLgr4 #3 or Lgr4 #4). Cy3 + and Cy3 - CML-LSK cells were purified at 3 days post-transduction and plated in a semi-solid methylcellulose medium. Data are the mean colony number +- s.d. ( n = 3) and are representative of three biologically independent experiments. ( P -value compared with control, unpaired two-sided Student''s t -test). The relevant FACS data are shown in Supplementary Fig. 12 . c Absolute numbers of LT-CML stem cells isolated from BM of the two hind limbs of Lgr4 +/+ tet-CML-affected mice (four males, one female) and Lgr4 Gt/Gt tet-CML-affected mice (five females) ( n = 5 biologically independent samples). Data are the mean absolute numbers +- s.d. of LT-CML stem cells ( P -value, unpaired two-sided Student''s t -test). (See S
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- Figure 4 Lung leukocyte infiltration during influenza infection is comparable between PAD4 WT and KO mice. Experimental setup as described in Figure 2 . (A-C) Five mice per group were analyzed at d3 or d7 post infection. Leukocytes were isolated from infected lungs. (A) Total cell numbers of lung-infiltrating leukocytes at d3 and d7 p.i. Each symbol represents an individual mouse, filled squares represent PAD4 WT, open squares depict PAD4 KO mice. (B+C) The subsets of infiltrating leukocytes were enumerated by flow cytometry both at d3 p.i. (B) and d7 p.i. (C). Gated populations are indicated at the bottom of the graph. Total numbers of infiltrating cells are shown. WT mice are depicted in white bars, KO mice as grey bars. Bars represent mean + SEM. (D) Lungs for histological examination were harvested at d8 p.i. (5 mice/group). Leukocyte infiltration was assessed on H&E stained paraffin sections. Sections from two representative mice per group are shown. The scale bar indicates 200 um. Data is representative of two independent experiments.
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- Fig. 4 Analysis of B cell development in bone marrow and spleen. A. FACS analysis of BM B cells. BM cells from WT and RhoF KO mice (6-7 weeks old, n = 5, male: 40%) were stained with antibodies against CD43, B220, BP1, and CD24 and analyzed by flow cytometry. The number shows the percentage (mean +- standard deviation) of the indicated subpopulation within the parent population; Fraction A (germline pro-B cells), fraction B (DJ-rearranged pro-B cells), fraction C (Early pre-B cells), fraction D (Late pre-B cells), fraction E (newly formed B cells), and fraction F (follicular-type recirculating B cells). The figures are representatives of three independent experiments. B. FACS analysis of B cells from the spleen. Splenocytes from WT and RhoF KO mice (6-7 weeks old, n = 6, male: 33%) were stained with antibodies against CD23, CD21, and IgM and analyzed by flow cytometry. The number shows the percentage (mean +- standard deviation) of the indicated subpopulation within the parent population; T1 (Transitional 1), T2 (Transitional 2), Fo (mature follicular B cells), and MZ (MZ B cells). C. The number of B cell subsets in BM was calculated by multiplying the total number of viable (trypan blue negative) BM cells by the fraction of the target population in viable (7AAD negative) cells. The data are shown as mean +- standard deviation (6-7 weeks old, n = 5, male: 40%). White bars: WT. Black bars: RhoF KO. D. The number of B cell subsets in the spleen was also calculated by multiplyin
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- Figure 2 Loss of Gata5 from renal endothelial cells leads to renal alterations. ( a ) GATA5 is expressed in the kidney as assessed by western blot performed on total kidney extracts. ( b ) Gata5 is essentially expressed in the glomeruli as assessed by qPCR on isolated glomeruli (Glo) and microdissected tubules (Tub) from Wt mice kidneys. ( n =3-5 per group). The results are reported as mean+-s.e.m. * P
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- Figure 5 NG2-cre, but not NG2-cre ERTM , targeted cells are the source of Scf in the bone marrow (a) Whole-mount sternum from NG2-cre/ iTdTomato/ Scf-GFP mice, anti-VE-cadherin. Representative images from 3 mice. Scale bars, 20 mum. (b) Representative FACS plot showing percentage of NG2-cre/ iTdTomato + cells within CD45 - TER119 - CD31 - Scf-GFP + cells. n=3 mice. (c-e) Analyses of LepR-cre/ Scf fl/- mice. (c) Numbers of HSCs (left) in BM and LSK cells in spleen (right). n=4 mice for cre (-), n=3 mice for cre (+). (d) FACS analyses of HSC (CD150 + CD48 - LSK) cell cycle with Ki-67 and Hoechst 33342 staining. n=5 mice for cre (-), n=6 mice for cre (+). (e) HSC localization relative to arterioles. Error bars: n=3 mice. P value has been calculated using n=272 HSCs for cre (-), 293 HSCs for cre (+) pooled from 3 mice per group. P =0.3402. (f-i) Analyses of NG2-cre/ Scf fl/- mice. (f) Numbers of total BM cells (left) and CD150 + CD48 - LSK HSCs (right) in BM. n=5 mice for cre (-), n=7 mice for cre (+). (g) Percentages of donor-derived cells after competitive reconstitution. n=5 mice for cre (-), n=7 mice for cre (+). (h) FACS analyses of HSC cell cycle with Ki-67 and Hoechst 33342 staining. n=6 mice for cre (-), n=7 mice for cre (+). (i) HSC localization relative to arterioles. Error bars: n=3 mice. P value has been calculated using n=224 HSCs for cre (-), 274 HSCs for cre (+) pooled from 3 mice per group. P =0.2872. (j-l) Analyses of NG2-cre ERTM / Scf fl/- mice. (j) Absolute nu
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- Figure 2 Splenic T cell hyperplasia and T cell size in p27 -/- Lck-Bcl-2 mice. A) Total splenic cells from mice of the indicated genotypes were isolated and stained with anti-B220-PE and anti-CD3-FITC antibodies as described in the Materials and Methods . The percentage of CD3-positive cells and the total number of splenic T cells (total # of splenic cells X %CD3) is shown. The data are representative of at least three mice from each genotype. B) The Mean Forward Scatter of CD3 positive splenic T cells was determined by staining with an anti-CD3 antibody as described in the Materials and Methods . The Mean+-SD of at least 5 mice is shown for the genotypes indicated. * P
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- Figure 7. NOX2 and FOXC1 Are Important for the Growth of Primary Human Myeloid Leukemia Cells (A) The Cancer Genome Atlas (TCGA) database for AML was used to analyze the expression levels of several NADPH-dependent oxidases (NOX1-NOX5), accessory subunits (NCF1, NCF2, NCF4, NOXA1, NOXO1, p22Phox, RAC1, and RAC2) as well as related oxido-reductase enzymes (dual-oxidases 1,2, A2). The reads per kilobase of transcript per million mapped reads (RPKM) values for each gene in a total of 188 AMLs are shown. (B) RNA-seq analysis was performed on functionally validated leukemic stem cells isolated from human primary AMLs. The RPKM value for each gene is shown. Unpublished data are used with permission. Additional supporting data are shown in Figure S7A . (C) Equal numbers of control or shNOX2-transduced primary AML cells were cultured in vitro in the presence of 10 ng/mL of IL-3, SCF, and FL3, and the relative percent expansion is reported for each specimen. n = 3, mean +- SD. *p < 0.05; **p < 0.01. (D) Control and shNOX2 primary AML cells were purified and cultured in vitro for 12 days. Annexin V, DAPI staining was performed to evaluate the degree of apoptotic cell death. A representative flow plot (left) and quantitation of 3 technical triplicates (right) are shown. Additional data are shown in Figure S7B . (E) The relative expression level of NOX2 , CEBP epsilon, Elane , and CTSG is shown in AML specimens in which NOX2 was knocked down using shRNAs. Additional supporting data are p
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- Figure 6 The characterization of Il-2 producers regulated by FX and TAC. FX stimulated Il-2 production in CD45R + CD25 + ILCregs. Some Il-2 + CD45R + CD25 + ILCregs were observed in TAC treated skin and others were CD45R - CD25 - cells. The panel is focused on the dermis.
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- Fig. 4. Macrophage-derived MCPIP1 regulates B-lymphocyte expansion in lymphoid organs. (A-D) Spleen sections were stained with anti-mouse IRF4, BLIMP1, CD95 and B220 antibodies and quantified by Adobe Photoshop software as percentage of positively stained high-power field (HPF) from eight mice per group; *** P
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- Fig. 1 Ubiquitous miR-210 over-expression attenuates the inflammatory response after hindlimb ischemia. A Representative immunofluorescence staining for CD45 (green), a common leukocyte marker expressed in all nucleated hematopoietic cells, of gastrocnemius muscle sections of Wt and Tg-210 mice, 7 days after ischemia. Nuclei were stained by Hoechst (blue). Images are presented as merge. Magnification x400. Calibration bar 50 um. B Quantification of CD45-positive cells/mm 2 ( n = 5, test T * P < 0.03). C Representative immunofluorescence staining for F4/80 (green), a pan-macrophage marker, in gastrocnemius muscle sections of Wt, and Tg-210 mice, 7 days after ischemia. Nuclei were stained by Hoechst (Blue). Images are presented as merge. Magnification 63 x 0.5. Calibration bar 50 um. D Quantification of F4/80-positive macrophages/mm 2 ( n = 6-7, ** P < 0.009). In both A , C insets show magnification of the indicated areas.
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- Figure 6 Assessments of organ damage and mouse metabolites during HLSA or recovery from HLSA. A , upper left panel , MMP-1, IL-1beta, and CRP levels in plasma had no significant change between the control group, HLSA group, and R-HLSA group. Upper right panel , CK-MB as a marker of heart injury in the plasma had no significant change between the control group, HLSA group, and R-HLSA group. Lower left panel , creatinine and BUN as markers of kidney injury in the plasma slightly increased in the HLSA group and returned to normal level after recovery from HLSA. Lower right panel , AST and ALT as markers of liver injury in the plasma showed no change between the control group, HLSA group, and R-HLSA group. Data were presented as mean +- SD (N = 6; ANOVA: ** p < 0.01). B , upper panel , food consumption and water consumption 3 days before and 3 days after a single ATP-induced HLSA depicted no change. Data were presented as mean +- SD (N = 6; ANOVA: p > 0.05). Lower panel , metabolomics analysis of scores plot from PCA analysis based on 1 H NMR data from the plasma, brain, liver, and kidney of the control group, HLSA group, and R-HLSA group. The PCA score showcased clusters correspond to metabolic patterns in different groups, with each point representing one sample. Circles represent 95% confidence interval for each score in each group (see also Figs. S5-S8 ). C , draining lymph nodes of mice that underwent HLSA once a day for 10 consecutive days were analyzed with FACS. CD44 and
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- Figure 5 CD4 surface expression of CD45RB and global glycosylation are altered in SLE123 mice. ( A and B ) Cell surface expression of CD45RB and total CD45 were measured by flow cytometry on CD4 + T cells. SLE123 demonstrated a downregulation of CD45RB on the cell surface as compared with B6 CD4 + T cells. There was no difference in pan-CD45 expression. Quantified in B . ( C ) CD45 is composed of an intracellular region that controls cytoskeletal binding and its phosphatase activity. The extracellular domain is composed of a region with fibronectin repeats that is heavily N-glycosylated. An alternatively spliced region is heavily O-glycosylated, and this region imparts unique functions to each CD45 isoform. Additionally, a sialic acid residue on the B portion of this region is essential for the binding of therapeutic aCD45RB. ( D and E ) We utilized lectins to detect the level of alpha-2,3-linked sialylation (MALII), O-linked glycosylation (Jacalin), and N-linked glycosylation (PHA-L) in CD4 + T cells from B6 and SLE123 mice. We determined SLE123 CD4 + T cells had reduced levels of alpha-2,3-linked sialic acids and O-glycosylation and an increase in N-glycosylation. Quantified in E . ( F ) Utilizing an antibody that detects a desialylated form of anti-CD45RB, we determined SLE123 CD4 + T cells possessed increased binding of this antibody compared with B6. ( G and H ) To determine the binding of therapeutic aCD45RB to B6 and SLE123 CD4 + T cells, we incubated splenocytes from
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- Figure 2. pIgR -/- mice spontaneously develop COPD-like lung pathology and adaptive immune activation. ( A ) Representative image of emphysema in an 18-month-old pIgR -/- and age-matched WT mouse (hematoxylin and eosin, scale bar = 50 mum). ( B ) Measurement of mean linear intercept (MLI), a morphometric measurement of emphysema, in WT and pIgR -/- mice at the indicated ages. ( C ) Representative image of small airway wall thickening in an 18-month-old pIgR -/- and age-matched WT mouse (Masson's trichrome, scale bar = 50 mum). ( D ) Measurement of VV airway , a morphometric measurement of small airway wall thickness, in WT and pIgR -/- mice at the indicated ages. ( E ) Example of a tertiary lymphoid structure (TLS) in an 18-month-old pIgR -/- mouse as indicated by immunostaining for B220. Scale bar = 50 mum. ( F ) Morphometric analysis of TLS area in lungs of WT or pIgR -/- mice at the indicated ages. ( G-J ) Quantification of total, CD45 + , CD19 + , CD3 + , CD4 + , and CD8 + cells in the lungs of 18-month-old WT and pIgR -/- mice by flow cytometry. ( B and D ) * = p
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- Fig. 7 Upregulation of PD-L1 by MTHFD2 is required for tumorigenesis. a - b A total of 2 x 10 6 ( a ) or 5 x 10 6 ( b ) Pan02 cells stably infected with lentivirus carrying indicated shRNAs or exogenous expressed PD-L1, were subcutaneously injected into athymic nude mice ( a ) or C57 mice ( b ), tumor volume was calculated every 7 days. Tumor xenografts at the 28th day in nude mice ( a ) or the 21th day in C57 mice ( b ) were shown. Data represent the means +- s.e.m ( n = 8 mice per group); p value (Student's t test, two-sided) with control is presented. c , PD-L1 mRNA levels in tumor tissues in C57 mice were analyzed by real-time PCR. The values are presented as mean +- s.e.m ( n = 3); p values (Student's t test, two-sided) with control or the indicated groups are presented. d The lysates of 8 pooled tumor tissues in C57 mice were subjected to immunoblotting analyses using the indicated antibodies. e Immunohistochemical staining was performed on tumor sections in C57 mice with anti-CD8 antibody. Representative images are shown. Scale bars, 50 mum. Histological semi-quantification was performed. f Cells digested from indicate tumor tissues in C57 mice were stained with anti-CD45 antibody and subjected to flow cytometric analyses (also see Supplementary Fig. 7b ). Representative images (1 out of 3 experiments) are shown. g A schematic model showing the role of MTHFD2 in tumor immune evasion. MTHFD2 promotes PD-L1 mediated tumor immune evasion through the folate-cycle-UTP-UDP-G
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- Figure 6 Colonic innate and adaptive immune cell responses following peroral C. coli infection of human microbiota-associated TLR4-deficient IL10 -/- mice. IL10 -/- mice (open symbols) and TLR4-deficient IL10 -/- mice (TLR4 -/- IL10 -/- ; closed symbols) with a human gut microbiota were either perorally infected with C. coli (circles) or received vehicle (mock; diamonds) on days 0 and 1 by gavage. Upon necropsy on day 21 post-infection, the average counts of ( A ) macrophages and monocytes (F4/80 + ), ( B ) T lymphocytes (CD3 + ), ( C ) regulatory T cells (FOXP3+) and ( D ) B lymphocytes (B220+) were microscopically determined in six high power fields (HPF, 400 times magnification) per mouse in immunohistochemically stained colonic paraffin sections. Medians (black bars) and numbers of examined mice (in parentheses), as well as levels of significance ( p -values) determined by the one-way ANOVA test and Tukey's post-correction or the Kruskal-Wallis test and Dunn's post-correction, are shown. Data were pooled from three independent experiments.
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- Figure 6 Serum CXCL13 levels in mouse ktx. Post ktx levels of serum CXCL13 at day 1 were significantly increased compared to baseline. A higher increase was observed in longer cold ischemia time (30 vs. 60 min cold ischemia time). Isogenic ktx with prolonged cold ischemia time of 60 min had significantly lower CXCL13 levels compared to allogenic ktx (A) . PAS stain at day 7 revealed enhanced cell infiltration in allogenic compared to isogenic ktx (B) . Double staining for CD3+ T-lymphocytes (green) and CD45R+ B-cells (red) was performed at day 7. More interstitial CD3+ T-lymphocytes were observed in allografts compared to isografts. Allografts exhibited scattered B-cells in interstitial tissue, but also clusters of CD45R+ cells. Isografts showed only few B cells in the interstitium at day 7 (B) (bar: 100 mum, n = 6 per group, one-way ANOVA * p < 0.05; ** p < 0.01; *** p < 0.001). BL, baseline.
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- Figure 7 Colonic immune cell responses upon oral C. coli infection of microbiota-depleted IL-10 -/- mice that had been challenged with human or murine fecal microbiota transplantations. On days -7, -6, and -5, mice were subjected to murine (open symbols) or human (closed symbols) fecal microbiota transplantations and challenged with a C. coli patient isolate (circles) or vehicle as mock controls (diamonds) on days 0 and 1 by gavage. On day 21 post-infection, the average numbers of ( A ) macrophages and monocytes (F4/80+), ( B ) T lymphocytes (CD3+), ( C ) regulatory T cells (FOXP3+), and ( D ) B lymphocytes (B220+) were determined in the large intestinal mucosa and lamina propria out of 6 high-power fields (HPF, 400x magnification) per mouse. Medians (black bars), significance levels ( p -values calculated by the Kruskal-Wallis test and Dunn's post-correction), and total numbers of mice under investigation (in parentheses) are shown. Results from four experiments were pooled.
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- Figure 5 Innate and adaptive immune cell responses in the colon following PACAP treatment of C. jejuni infected secondary abiotic IL-10 -/- mice. Mice were perorally infected with C. jejuni strain 81-176 by gavage on day (d) 0 and d1, and subjected to intraperitoneal treatment with either synthetic PACAP or vehicle (mock) from d2 until d5 post-infection. On d6 the average numbers of ( A ) macrophages and monocytes (F4/80 + ), ( B ) T lymphocytes (CD3 + ), ( C ) regulatory T cells (FOXP3 + ) and ( D ) B lymphocytes (B220 + ) were quantitated in six high power fields (HPF) of colonic paraffin sections applying immunohistochemistry. Naive mice were used as negative controls. Box plots indicate the 75th and 25th percentiles of the median (black bar within boxes). The total range, the significance levels ( p values calculated by the one-way ANOVA test followed by the Tukey post-correction test for multiple comparisons) and the total numbers of mice under investigation (in parentheses) are indicated. Results pooled from four independent experiments are shown.
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- Figure 1 Ibrutinib promotes renal fibrosis after unilateral ureteric obstruction (UUO). ( A ) Design of the study. ( B - E ) Representatives images and quantifications of Fibronectin ( B ), Sirius Red ( C ), B220 ( D ) and F4/80 ( E ) staining in sham and UUO mice receiving ibrutinib (Ibru) or vehicle (Veh). Data are represented as means +- SEM. *p < 0.05 (Mann-Whitney test; n = 6 mice per group); ns, not significant.
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- Figure 3 Ibrutinib promotes rhabdomyolysis-induced renal fibrosis. ( A ) Design of the study. ( B ) Kaplan-Meier survival curves after glycerol injection in mice receiving ibrutinib (Ibru) or vehicle (Veh) from day 3. ( C ) Blood urea nitrogen (BUN) 20 days after glycerol injection. ( D - G ) Representatives images and quantification of Sirius Red ( D ), Fibronectin ( E ), F4/80 ( F ) and B220 ( G ) staining 20 days after glycerol injection in mice receiving ibrutinib (Ibru) or vehicle (Veh) from day 3. Values are expressed as mean +- SEM. * p < 0.05, compared with the vehicle group (Mann-Whitney test, n = 5-10 mice); ns, not significant.
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- Fig. 2 Generating immuno-CRISPR (iCR) mice and evaluating the CRISPR-mediated modifications by sequencing. ( A ) Model describing the experimental setup where CD45.1 + Lin- BM cells were modified by CRISPR targeting Zap70 and grafted into irradiated CD45.2 + recipients. ( B ) Flow cytometry analysis of cells in the blood of Zap70 iCR mice and WT control mice eight weeks post transplantation. Cells gated on viable, CD45.1+, single lymphocytes. ( C ) Quantification of B and T cells in the blood of WT and Zap70 iCR mice in (B). ( D ) Analysis of the level of mutations in the sgRNA targeted Zap70 region in the BM cells used for transplantation, total cells from the blood, as well as in B and T cells sorted from the spleen of Zap70 iCR mice and WT control mice 8 weeks after engraftment. ( E ) Model describing the experimental setup where a secondary transplantation was used to amplify the population of successfully modified mice. ( F ) Analysis of the level of mutations in the sgRNA targeted GFP region in blood cells of the GFP iCR mice four weeks after transplantation, in an experiment with low efficiency. One mouse showed good knockout efficiency (labeled in orange) and was used as BM donor for secondary transplantation. ( G ) Kinetics of the level of mutations of GFP in the secondary iCR mice. ( H ) As examples, representative flow cytometry plots and ( I ) GFP + cell population percentage of alveolar macrophages, B1 cells, neutrophils and germinal center B cells from secondary
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- Figure 4 Proof-of-mechanism study for TAX2 use as an anti-tumor immunomodulatory drug. The effects of TAX2 peptide treatment on the activation of an anti-tumor immune response were investigated in two syngeneic models of epithelial ovarian carcinoma. We inoculated 5 x 10 6 ID8 cells either subcutaneously or intraperitoneally to C57BL/6JRj mice in order to mimic primary tumor growth ( a - h ) and metastatic dissemination (peritoneal carcinosis, i - l ), respectively. TAX2 treatments (30 mg * kg -1 BW) vs. vehicle injections (0.9% NaCl) were performed thrice weekly for 4 and 8 weeks for the s.c. and i.p. ID8 models, respectively ( n = 11 per group for both models). ( a - h ) IHC staining of s.c. ovarian carcinoma allografts for the analysis of the vascular/lymphovascular features, as well as immune-cell-infiltration profile. ( a ) Representative microphotograph for CD31 immunostaining of intra-tumor vascular structures. Histogram displays results of automated quantification for the percent of CD31-positive pixels across the whole tumor section (mean +- SEM, Mann-Whitney U test; n.s., not significant). ( b ) Lymphatic vessel density analysis. Microscopic views are shown, while histogram displays the number of Lyve-1-positive functional lymphatic vessels in 5 high power fields (HPF), as determined by a pathologist who was blinded to the treatment (mean +- SEM, Mann-Whitney U test, * p < 0.05). ( c - f ) Macroscopic views (x20 magnification) of s.c. tumor allograft sections after
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- Figure 8. Increased IL-2 production impairs T follicular helper (Tfh) cell formation and the germinal centre response. Assessment of the Tfh cell and germinal centre response in Il2 cre/+ ; Rosa26 stop-flox-Il2/+ transgenic mice that do not switch off IL-2 production, and Il2 cre/+ ; Rosa26 +/+ control mice 12 days after influenza A infection. Flow cytometric contour plots ( A ) and quantification of the percentage of CXCR5 high PD-1 high Foxp3 - CD4 + Tfh cells in the mediastinal lymph node ( B ) and spleen ( C ). Flow cytometric contour plots ( D ) and quantification of the percentage of Bcl6 + Ki67 + B220 + germinal centre B cells in the mediastinal lymph node ( E ) and spleen ( F ). The height of the bars indicates the median, each symbol represents one mouse, data are pooled from two independent experiments. p-Values calculated between genotype groups by Mann-Whitney U test.
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- Fig. 2. Effect of different PVA hydrolysis rates and molecular weights on ex vivo cultured human cord blood CD34 + hematopoietic stem and progenitor cells. a , Human cord blood CD34 + cells were cultured in PVA media and cell numbers were counted at day 14. Three independent experiments were performed with cord blood CD34 + cells derived from different donors. Cell cultures were started from 7 x 10 3 (Exp.1) or 5 x 10 3 (Exp. 2 and 3) CD34 + cells. Mean +- S.D is shown. Statistical significance was calculated using ANOVA followed by Tukey-Kramer; *p < 0.05, **p < 0.01. b , Proliferation of the two different CD34 + cell fractions after 7-day culture in PVA-containing media. Fifty cells were sorted into four wells in a 96-well plate and cell numbers in each well were counted at 7-day. Statistical significance was calculated using ANOVA and Tukey-Kramer. **p < 0.01.
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- Figure 8 Establishment of adaptive immunity in the infected lungs of WT and CXCL5 -/- mice. (A) Lung lobe sections from WT and CXCL5 -/- mice were stained at 10 d.p.i. with an anti-B220 antibody and hematoxylin (x30 magnification). (B) The positively stained area (iBALT structure) in each lung lobe section was calculated using CaseViewer software (3DHISTECH). The total area of iBALT per lung section was calculated from 4 mice per group (3 lung lobe sections per mouse). (C) Lung sections were stained with anti-B220 (red) and anti-CD4 (yellow) antibodies for immunofluorescence detection. The immunofluorescence sections were counterstained with DAPI (blue). (D) GO functional analysis of upregulated genes in the infected lungs of CXCL5 -/- mice compared to WT mice at 7 and 10 d.p.i. The methods and criteria for filtration of differentially expressed genes are described in the MATERIALS AND METHODS section. (E) Mice were infected with H1N1 or H3N2 (3000 CCID 50 ), and the body weight loss (n=12) of the mice was monitored until 2 weeks post infection. WT-1 H1N1: WT mice infected with H1N1; WT-1 H3N2: WT mice infected with H3N2; CXCL5 -/- 1 H1N1: CXCL5 -/- mice infected with H1N1; CXCL5 -/- 1 H3N2: CXCL5 -/- mice infected with H3N2; WT-2 H1N1: WT mice recovered from H1N1 infection and infected with H1N1; WT-2 H3N2: WT mice recovered from H1N1 infection and infected with H3N2; CXCL5 -/- 2 H1N1: CXCL5 -/- mice recovered from H1N1 infection and infected with H1N1; CXCL5 -/- 2 H3N2: CXC
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- Figure 3. Histone deacetylase 3 (HDAC3)-deficient CD4 + T cells are capable of differentiation, but produce fewer cells than wild-type (WT). ( a ) In vitro differentiation assays were performed to examine differentiation into the T h 1, T h 2, T h 17, and T reg lineages characterized by transcription factor expression. Splenocytes were harvested and magnetically enriched for naive (CD44 - ) CD4 + T cells by negative selection. Cells in all assays were stimulated with 2 ug/ml plate-bound alphaCD3 and 0.5 ug/ml alphaCD28 for 4 days. For T h 1 differentiation, 1 ug/ml alphaIL-4 antibody and 10 ng/ml of IL-12 were added to the media. For T h 2 differentiation, 1 ug/ml of each alphaIFNgamma and alphaIL-12 antibody, as well as 10 ng/ml of IL-4 was added to the media. For T h 17 differentiation, media was supplemented with 10 ug/ml of alphaIFNgamma and alphaIL-4 antibody as well as 10 ng/ml of rIL-23, 5 ng/ml TGF-beta1, and 20 ng/ml IL-6. For T reg differentiation, media was supplemented with 10 ug/ml alphaIFNgamma and alphaIL-4 antibody as well as 2 ng/ml TGF-beta1, and 2 ng/ml interleukin-2 (IL-2). Unstimulated control samples did not receive alphaCD3/alphaCD28 stimulation, but did receive 10 ng/ml IL-7 to maintain cell survival during culture. Bar plots on the right show % of cells from the total culture that are positive for the transcription factor +- standard deviation (SD), total cell number +- SD, and geometric mean of expression +- SD ( n = 2-4 mice/group from two to three
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- Figure 6 MALP ablation blocks bone marrow recovery after radiation. ( A ) Representative fluorescence images of Td + cells, Perilipin + LiLAs, and CD45 + hematopoietic cells in femoral bone marrow of Adipoq/Td/DTR mice after receiving 2 weeks of vehicle (Veh) or DT injections with or without prior radiation. Scale bar: 20 mum (top), 100 mum (middle), and 20 mum (bottom). ( B ) Quantification of CD45 + cells per bone marrow area. n = 3-6 mice/group. ( C ) Bone marrow cells were flushed from femurs and counted. n = 3-8 mice/group. ( D ) Cell counts of hematopoietic lineage cells in the bone marrow. n = 3-11 mice/group. B cells = B220 + , T cells = CD3 + , myeloid cells = Gr1 + and/or Mac1 + . ( E ) Cell counts of HSPCs. n = 3-11 mice/group. LK, Lineage - cKit + , LSK, Lineage - Sca1 + cKit + , SLAM LSK, Lineage - Sca1 + cKit + CD48 - CD150 + , MPP, Lineage - Sca1 + cKit + CD48 + CD150 - . ( F ) Representative fluorescence images of Adipoq/Td/DTR femoral bone marrow with Emcn staining (vessels). Arrows point to Td + pericytes. Scale bar: 20 mum. ( G ) Quantification of bone marrow vessel diameter, density, and area. ( H ) The number of pericytes per vessel length (VL) was measured. n = 3-4 mice/group. ( I ) The percentage of Emcn + endothelial cells in bone marrow was measured by flow cytometry. n = 3-4 mice/group. ( J ) qRT-PCR analysis of hematopoietic and angiogenic factors in sorted Td - and Td + cells from bone marrow before and after radiation. n = 4 mice/group. Statistica
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- Figure 2. The miR-92 deficient mir-17-92 cooperates with c-Myc to promote highly aggressive B-lymphomas. ( A ) The percentage of IgM positive and IgM negative B-lymphomas was calculated for each genotype ( Emu-myc/MSCV, n = 10 ; Emu-myc/17-92, n = 9; Emu-myc/17-92Delta92, n = 10; Emu-myc/17-92Mut92, n = 10). ( B ) The Emu-myc/17-92Mut92 and Emu-myc/17-92Delta92 mice developed high grade B-lymphomas that were frequently disseminated into the liver. When compared to Emu-myc/MSCV and Emu-myc/17-92 mice, Emu-myc/17-92Mut92 and Emu-myc/17-92Delta92 lymphomas gave rise to more liver dissemination, as indicated by H&E and B220 staining. ( C ) Emu-myc/17-92Mut92 and Emu-myc/17-92Delta92 lymphomas exhibited a decreased apoptosis compared to Emu-myc/MSCV or Emu-myc/17-92 lymphomas. Representative lymphomas were stained for H&E, cleaved caspase-3 and PCNA. Arrow, 'starry sky' feature of apoptotic lymphoma cells; arrowhead, apoptotic cells with positive staining for cleaved caspase-3; scale bar, 50 mum. ( D and E ) Apoptosis was quantitatively measured in representative lymphomas of each genotype using the 'starry sky' features ( D ) and cleaved caspase-3 staining ( E ). *p < 0.05, **p < 0.01, ***p < 0.001. DOI: http://dx.doi.org/
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- Lenvatinib insignificantly affects the immune microenvironment in NPC in humanized NSG mice. A Schematic diagram of the establishment of humanized NSG mice. B Representative FACS analysis of human CD45 + cells in mouse peripheral blood. Human CD45 + cell percentage greater than 25% was considered successful in modeling. C - E Tumor growth ( C ), tumor weights ( D ) were measured in vehicle-, anti-VEGF-, and lenvatinib-treated NPC tumors. The tumor inhibition ratio were calculated ( E ) ( n = 3 samples per group). F Representative micrographs of Ki67 + proliferative cells and cleaved caspase-3 + apoptotic cells in vehicle-, anti-VEGF-, and lenvatinib-treated NPC tumors. Scale bar = 50 mum. Quantification of Ki67 + , cleaved caspase-3 + signals, and PA index in vehicle-, anti-VEGF-, and lenvatinib-treated NPC tumors. ( n = 8 random fields per group) G Representative micrographs of CD31 + microvessels and CA9 + hypoxic areas in vehicle-, anti-VEGF-, and lenvatinib-treated NPC tumors. Scale bar in upper panel = 100 mum, scale bar in lower panel = 50 mum. Quantification of CD31 + tumor vessel parameters and CA9 + signals in vehicle-, anti-VEGF-, and lenvatinib-treated NPC tumors ( n = 8 random fields per group). H Quantification of hCD45 + hCD14 + population, hCD45 + hCD19 + population, hCD45 + hCD3 + population, and hCD45 + hCD56 + population in the NPC TME ( n = 3 samples per group). I Quantification of mCD45 + mCD11b + mF4/80 + population, mCD45 + mB220 + population, mCD45 + mC
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- Figure 6 Disease Modeling of herPAP Using miPAP iPSCs (A) Number of CD41 + progenitor-derived colonies in methylcellulose-based clonogenic assays employing complete (IL-6, erythropoietin, SCF, IL-3, and supplemented with 20 ng/ml human G-CSF + 20 ng/ml murine GM-CSF) or basic medium (50 ng/ml murine GM-CSF only; independent experiments, n = 3 miPAP1 and 2, n = 2 CD45.1 iPSC, mean +- SD) and representative pictures of colonies. Scale bars, 500 mum. (B) GM-CSF clearance assay comparing miPAP-Mphi with CD45.1(10.3) iPSC-Mphi, BMlin - -Mphi, and no cells incubated with 2 ng/ml GM-CSF: at indicated time points (0, 4, 10, 24, and 30 hr) GM-CSF concentrations in supernatants were analyzed by ELISA, normalized to 0 hr (independent experiments, n = 3 BMlin - and CD45.1 iPSC, n = 2 miPAP1 and 2, mean +- SD). (C and D) Flow cytometry plots of STAT5 phosphorylation levels upon stimulation with mGM-CSF (C) and (D) summary of mean fluorescence intensity (MFI) data (independent experiments, n = 3 BMlin - and CD45.1 iPSC, n = 2 miPAP1 and 2, mean +- SD). ns, not significant; ** p
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- Fig 6 Tn P acts systemically during the induction phase modulating DCs and induces regulatory cells. Splenocytes from EAE mice treated with vehicle or Tn P (n = 5/group) were isolated at day 7, and analyzed. A ) The MFI of MHC class II, CD40, CD80, CD86 in cDC (CD11c+CD11b+) and B ) MFI of PDL-1 and PDL-2 in pDC (CD11c+B220 low ) were analyzed by flow cytometry (50,000 events). C ) The percentage of CD4+ cells, D ) and the MFI of CD18, CD40L, and CD69 in the CD4+ gate were analyzed by flow cytometry. E , F , G ) The percentages of FOXP3-positive CD4+CD25+ Treg, IL-4-positive CD4 Th2 cells and CD5-positive CD19+CD1d+ Breg cells were analyzed by flow cytometry. Values in the bar graphs are the mean +- SEM. * p < 0.05 and ** p < 0.01 compared with vehicle-treated EAE-mice.
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- Fig. 1 In vivo depletion of Cdkn1a rescues GC formation in Ezh2 -/- mice. Ezh2 fl/fl , Cdkn1a -/- , Ezh2 fl/fl ;Cgamma1-cre and Ezh2 fl/fl ;Cgamma1-cre; Cdkn1a -/- littermate mice were immunized with SRBC to induce germinal center (GC) formation and were killed 10 days later. a Flow cytometry plot of one representative mouse spleen per group. The gated area shows the percentage of GC B cells (GL7 + FAS + ) within live B cells (B220 + DAPI-, see Supplementary Fig. 2A for gating strategy). b Average of GC B populations of each group of mice quantified by flow cytometry as in a ( n = 7 mice per group). c Formalin fixed paraffin embedded splenic tissue was stained for PNA, Ki67, EZH2, and B220. One representative picture of three spleens analyzed per group is shown. d - f Quantification of PNA staining from c ( n = 3 spleens per group). d ""#GC/spleen section"" is the count of all GC per spleen section. e ""GC area/total spleen area"" is the quantified area of each individual GC divided by the total area of the spleen section. f ""Total GC area/total spleen area"" is the sum of all GC quantified areas in a certain section divided by the total area of that spleen section. g Splenocytes were permeabilized and stained for GC B and EZH2 using a fluorochrome-conjugated anti EZH2 antibody. The gated area shows the percentage of GC B cells (GL7 + FAS + ) within live B cells (B220 + DAPI-, see Supplementary Fig. 2A for gating strategy) that are EZH2 positive. The flow plot shown is repre
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- Fig. 3 Characterization of a 3D B cell follicular organoid to model the GC reaction. a Scheme of 3D B cell follicular organoid fabrication. Splenic B cells are co-encapsulated with 40LB stromal cells and IL4 into an RGD-presenting nanocomposite hydrogel that contain gelatin ionically cross-linked with synthetic silicate nanoparticles. b Representative fluorescence pictures of splenic GFP B cells in 3D organoid culture. c Flow cytometry plot of a 3D B cell follicular organoid. The gated area on the top shows the live B cells (B220 + DAPI-) and on the bottom plot, the organoid GC B cells (GL7 + FAS + ) within live B cells. d Average of GC B populations of organoids quantified as in c . e Average of percentage of proliferating organoid GC B populations, quantified as indicated in Supplementary Fig. 4B . f MFI of proliferation dye from e . g Flow cytometry plot of GC B cells stained with annexinV. h Average of percentage of apoptotic GC B cells quantified as in g . i RNA-seq profiles of organoid GC B cells after 4 and 6 days in culture (organoid GCB d4, n = 4 spleens, and d6, n = 3 spleens) were projected into the principal component space defined by GC B cells sorted from immunized mice (in vivo GCB, n = 6 mice), CD138 + plasma cells (in vivo PC, n = 6 mice) and FAS-GL7-IgD + B220 + naive B cells (NB, n = 3 mice). j , k Heat maps of gene expression level of GC B cells showed in i , represented as log2 ratio relative to mean naive B cells j and to mean plasma cells k . PC1/2 = pr
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- Fig. 4 CDKN1A repression by EZH2 is required for GC B cell cycle progression. a - f Organoids were generated using B cells isolated from Ezh2 fl/fl ;Cgamma1-cre, Cdkn1a -/- , Ezh2 fl/fl ;Cgamma1-cre; Cdkn1a -/- and Ezh2 fl/fl control mice ( n = 3 mice per group) and were harvested for flow cytometry analysis after 4 days in culture. a Flow cytometry plots of representative organoids from each genotype. The gated area shows the percentage of organoid GC B cells (GL7 + FAS + ) within live B cells (B220 + DAPI-). b Average of percentage of organoid GC B populations quantified by flow cytometry as in a ( n = 3 biological replicates per group). c Organoids received a BrdU pulse of 2 h before harvest. Cell cycle was analyzed by BrdU staining and 7AAD to measure DNA content. The representative gated area shows the percentage of organoid GC B cells (GL7 + FAS + B220 + DAPI-) that are in S phase (BrdU + ). d Average of percentage of organoid GC B populations in S phase of each group of genotype quantified by flow cytometry as in c ( n = 3 biological replicates per group). e Organoid cells were permeabilized and stained for EZH2 and GC markers GL7, FAS, and B220 to identify organoid GC B cells. The flow cytometry plot shows one representative sample per genotype group. f MFI of EZH2 in organoid GC B cells ( n = 3) quantified by flow cytometry as in e . g - m Organoids were generated using B cells isolated from 3 Cdkn1a +/+ and 3 Cdkn1a -/- mice. g Scheme of EZH2 inhibitor GSK343 and Br
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- Figure 1. VEGF and bFGF promote the differentiation of MSCs into EC-like cells. (A) Expression of MSC surface markers was confirmed using flow cytometry. (B) Morphological features of isolated MSCs and MSC-derived EC-like cells following induction as observed under an inverted microscope. The red and white arrows indicate spindle-shaped and polygon-shaped cells, respectively. Magnification, x20. (C) Factor VIII expression was detected in MSC-derived EC-like cells via immunohistochemistry and light microscopy with brown staining indicating the positive signal. VEGF, vascular endothelial growth factor; bFGF, basic fibroblast growth factor; MSCs, mesenchymal stem cells; EC, endothelial cell; Ctrl, control; CD, cluster of differentiation.
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- Fig. 1 Loss of miR-143/145 results in reduced LT-HSC frequency. a Frequency of long-term-HSC (LT-HSC, CD45 + EPCR + CD48 - CD150 + ), LSK/short-term HSC (Lin - Sca1 + c-Kit + ), common myeloid progenitors (CMP, Lin - Sca1 - c-Kit + CD34 + CD16/32 lo ), granulocyte-macrophage progenitors (GMP, Lin - Sca1 - c-Kit + CD34 + CD16/32 hi ), and megakaryocyte-erythrocyte progenitors (MEPs, Lin - Sca1 - c-Kit + CD34 - CD16/32 lo ) in the marrow of 8-12-week- old wild-type (WT), miR-143/145 +/- , and miR-143/145 -/- mice, as analyzed by flow cytometry (median +- 1.5 IQR, WT n = 11, 143/145 +/- n = 9, 143/145 -/- n = 8). b Marrow cellularity (2 femurs and 2 tibias) (WT n = 3, 143/145 +/- n = 4, 143/145 -/- n = 8). c Colony-forming unit (CFU) assay of marrow cells from WT and miR-143/145 -/- mice (mean +- SEM, WT n = 11, 143/145 -/- n = 10). d Primary CFU cells were replated in equal proportions per condition, normalized to the number of input cells, to generate secondary CFUs (mean +- SEM, WT n = 9, 143/145 -/- n = 7). e Estimate of HSC frequency in WT and miR-143/145 -/- mice by limiting dilution assay. Shown is a log-fraction plot of the limiting dilution model. The slope of the line is the log-active cell fraction. The dotted lines give the 95% CI
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- Figure 1. Intestinal immune cell responses upon NAP treatment of human microbiota-associated mice suffering from subacute ileitis. Subacute ileitis was induced in human microbiota-associated (hma) mice on day 0 by peroral low-dose T. gondii infection as described in Materials and Methods section. Hma mice were then treated with synthetic NAP (crossed circles) or placebo (PLC; black circles) from day 1 until day 8 postinfection (p.i.). At day 9 p.i., the average numbers of (A) ileal and (B) colonic T lymphocytes (positive for CD3) and of (C) colonic B lymphocytes (positive for B220) were determined microscopically in immunohistochemically stained intestinal paraffin sections from six high power fields (HPF, 400x magnification) per animal. Naive mice (i.e., without ileitis and without treatment; white circles) served as negative controls. Numbers of animals (in parentheses), means, and significance levels ( p values) determined by Mann-Whitney U test are indicated. Data shown were pooled from four independent experiments
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- Figure 4 Surgical denervation of young bone marrow induces premature HSC and niche aging (a) Schematic illustration of surgical denervation experiment. The sciatic and femoral nerves were transected in young (2 months old) C57BL/6 mice and the mice were analyzed 4 months post surgery, at 6 months of age. (b) Absolute numbers of HSCs (lineage - Sca-1 + c-Kit + CD48 - CD150 + ) isolated from Sham and Denervated (Den) femurs (n=7 mice). (c, d) Total peripheral blood (CD45.2 + ), blood myeloid (Mac-1 + CD45.2 + ) and blood B cell (B220 + CD45.2 + ) donor chimerism at the indicated time points post transplantation (c) and bone marrow donor chimerism (Total CD45.2 + (BM); Myeloid (My), Mac-1 + CD45.2 + ; B cell (B), B220 + CD45.2 +; and T cell (T), CD4 + /CD8 + CD45.2 + ) 5 months after primary transplantation of 200 HSCs derived from either sham or denervated (Den) femurs and transplanted in competition with young BM competitor cells (n=4 sham, 6 denervated mice) (left) and 5 months after secondary transplantation of 3 x 10 6 bone marrow from primary recipients (right) (d). (e) Left, representative confocal z -stack projections of HSCs sorted from sham or denervated femurs and stained with Cdc42, Tubulin and DAPI. Scale bar, 10 mum. Right, quantification of the percentage of Cdc42 and Tubulin polarized HSCs out of total HSCs scored (total of 356 sham and 353 denervated HSCs isolated from 4 mice per group). (f) Left, representative confocal z -stack pro
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- Figure 1 ATF3 maintains intestinal homeostasis. (A) Comparison of colon length between naive mice as indicated. (B) Colon crypts from mice were isolated by shaking colon fragments in EDTA and counted under light microscopy. (C) Flow cytometry analysis of Ki67 and CD24 expression in ileum crypts, gated on the CD45 - EpCAM + populations, from the indicated naive mice. (D) Representative micrographs showing intestinal organoids derived from naive mice. (E) Quantitative real-time PCR analysis of cell cycle genes in naive ileum organoids at day 6 of culture (""n"" indicates organoids derived from 4 mice each group). (F) Representative confocal images of whole mount tissues with co-immunofluorescence staining of UEA-1 and WGA in naive ileum villi. Results were from at least two independent experiments and ""n"" refers to the number of mice unless indicated otherwise. All mice were at the age of 2~3 months old when analyzed. Statistical analysis was done using Multiple T -test on Prism software. * P < 0.05, ** P < 0.005, *** P < 0.0005.
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- Figure 7 ATF3 promotes IL-22-induced STAT3 phosphorylation by suppressing phosphatases. (A) Freshly isolated ileum crypts, or (B) ileum organoids at day 6 of culture, were stimulated with IL-22, followed by fixation and intracellular staining of phospho-STAT3, and analyzed by flow cytometry. Western blot analysis of (C) IL-22-stimulated CMT93 cells, or (D) IL-22-stimuated colon fragments isolated from the indicated mice, for the expression of the indicated proteins. (E) Quantitative real-time PCR analysis of IL-22R1 and IL-10R2 mRNA levels in freshly isolated ileum crypts from mice. (F) Flow cytometry analysis of IL-22R1 in freshly isolated ileum crypt cells gated on the CD45 - EpCAM + population. (G,H) Western blot analysis of unstimulated or IL-22-stimulated CMT93 cells for the indicated proteins. ATF3 -/- CMT93 cells with SHP2 knockdown (ATF3 -/- SHP2 KD ) were indicated. Images were representative of four independent experiments (G-H) . Results were from two independent experiments (A-F) . ""n"" refers to the number of mice analyzed (A,B,E,F) . Statistical analysis was done by multiple comparison in Two-way ANOVA test using Prism software. * P < 0.05, ** P < 0.005, *** P < 0.0005.
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- Figure 2 Tpl2 didn't affect peripheral immune activation during FH pathogenesis. The splenic cells or peripheral blood immune cells were isolated from P. acnes -primed WT and Tpl2 -KO mice at day 3, 5, and 7 as described in Materials and methods ( n = 4 mice/group), and subjected for flow cytometry analysis. (A-F) Flow cytometry analysis of the frequencies and absolute numbers of CD4 + T cells, CD8 + T cells, B220 + B cells, CD11c + dendritic cells (A,B) , CD4 + Foxp3 + Treg cells (C,D) , and IFN-gamma- and TNF-alpha-producing pathogenic Th1 cells (E,F) in the spleens of WT and Tpl2 -KO mice at day 7 after P. acnes priming. Data are presented as representative plots of the frequencies of immune cell subpopulations (A,C,E) and a summary graph of the cell frequencies or absolute cell numbers (B,D,F) . (G-J) Flow cytometry analysis of the frequencies and absolute numbers of CD4 + T cells in the spleens (G,H) or peripheral blood (I,J) of WT and Tpl2 -KO mice at day 3, 5, and 7 after P. acnes priming. Data are presented as representative plots of the frequencies of immune cell subpopulations (G,I) and a summary graph of the cell frequencies or absolute cell numbers (H,J) . Results are mean +- SD from three independent experiments.
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- Figure 3 Tpl2 reduced liver infiltration of pathogenic CD4 + T cells. Liver-infiltrating immune cells were isolated from P. acnes -primed WT and Tpl2 -KO mice at day 3, 5, and 7 as described in Materials and methods ( n = 4 mice/group). (A-G) Flow cytometry analysis of the frequencies and absolute numbers of CD4 + and CD8 + T cells, B220 + B cell, CD11c + dendritic cells (A-C) , CD4 + Foxp3 + Treg cells (D,E) , and IFN-gamma- and TNF-alpha-producing pathogenic Th1 cells (F,G) in the livers of WT and Tpl2 -KO mice at day 7 after P. acnes priming. Data are presented as representative plots of the frequencies of immune cell subpopulations (A,D,F) and a summary graph of the frequencies and absolute cell numbers (B,C,E,G) . (H,I) Flow cytometry analysis of the frequencies and absolute numbers of CD4 + T cells in the livers of WT and Tpl2 -KO mice at day 3, 5, and 7 after P. acnes priming. Data are presented as representative plots of the frequencies of immune cell subpopulations (H) and a summary graph of the absolute cell numbers (I) . Results are mean +- SD from three independent experiments. Two-tailed Student's t -tests were performed. * P < 0.05; ** P < 0.01.
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- Figure 5 MNP FcgammaR A:I Ratio Modulates Intestinal Inflammation (A) Weight loss in co-housed sex-matched WT (black circles, n = 8) and Fcgr2b -/- (red circles, n = 9) mice after a single 6-day course of 2% DSS. Means +- SEM are indicated. Data are representative of 3 independent experiments. (B) Confocal microscopy of colonic CD45 + leukocytes in mice treated as in (A). Data are representative of 2 independent experiments. (C) Flow-cytometric quantification of colonic neutrophil frequency (top) and absolute neutrophil counts (bottom) in WT and Fcgr2b -/- colons at day 25 after aDSS (n = 5-7 per group) or in healthy controls (day 0) (n = 3-5 per group). Medians are indicated. Data are representative of 3 independent experiments. (D) Pro-IL-1beta expression by colonic CD11b + CX3CR1 + MNPs at day 25 after aDSS (n = 5-7 per group). (E) Frequency of pro-IL-1beta expression within the Ly6C hi MHC-II - monocyte and Ly6C + MHC-II int macrophage populations as shown in aDSS (n = 5-7 per group) versus controls (day 0) (n = 3-5 per group). Medians are indicated. Data are representative of 3 independent experiments. (F) Chemokine mRNA levels in flow-sorted Ly6C lo MHC-II hi colonic macrophages at day 21 after aDSS (n = 4-5 per group). Data normalized to WT mRNA levels. Medians are indicated. (G and H) Weight loss (G) and clinical colonic and lymphoid organ features (H) of co-housed sex-matched M-TG (green circles, n = 5 or 6) and N-TG littermate controls (black circles, n = 6) after a
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- Fig. 6 TLR2 + macrophages, pDCs, and PB in the small intestine bind PSA and induce IL-10 secretion. Gating strategy for mononuclear cells isolated from a duodenum (Duod) and b ileum (Ile) of WT and Rag mice analyzed for binding of fluorescent A488-conjugated PSA (left two plots). CD45 - intra-epithelial cells (CD45 - IEC: middle histogram) and CD45 + gated intra-epithelial leukocytes (CD45 + IEL: second right histogram) isolated from the a Duod and b Ile of WT (red) and Rag (blue) mice were analyzed for reactivity to PSA-A488. CD45 + CD11c - B220 + B cells, PDCA1 + B220 + CD11c + pDCs and CD138 + B220 + PB and B220 low PC isolated from a Duod and b Ile of WT mice were analyzed for PSA reactivity (right histogram). Flow cytometry plots show CD11c + PDCA1 - cDC, PDCA1 + B220 + pDC, and B220 + CD19 + B cells isolated from spleen, MLN, PP, IEL, or LP of WT mice were stimulated with c PSA or d LTA-SA (TLR2 agonist) and analyzed for IL-10 expression. e Plots summarize data from c and d and show % IL-10 + B cells (left), cDCs (middle), and pDCs (right) from spleen, MLN, PP, IEL, and LP stimulated with PSA or TLR2 (LTA-SA) ( n = 3 mice). All data show mean +- SEM
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- Figure 2 Analysis of the B lymphocyte populations expanded in TRAF3/BCL2 double-tg mice with lymphoid dyscrasias. Three-color flow-cytometry analysis was performed to determine the phenotype of expanded B lymphocyte populations. Gating of the expanded population was based on the CD45R/B220 and FSC plot of each sample analyzed and is indicated in the figure. The surface molecules analyzed are indicated in the plots, as well as the percentage of cells found in each quadrant. The quadrants settings were selected based on the staining of isotype-controls (not shown). The tissue source where the analyzed lymphocytes were extracted from and the type of B cell malignancy developed by the TRAF3/BCL2 double-tg mice, according to the flow-cytometry and immunohistochemical analysis, is indicated in the figure.
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- Figure 4 Ptpn6 deletion drives robust anti-tumor immunity in two immune-rich syngeneic tumor lines. (A) Flow cytometric analysis of live CD45 + cells from B16F10 melanoma tumors isolated from tamoxifen-treated Ptpn6 fl/fl and Ptpn6 fl/fl ERT2-cre mice at day 14 post tumor implantation. (B) B16F10 tumor volume measurements in tamoxifen-treated Ptpn6 fl/fl ERT2-cre and Ptpn6 fl/fl mice. Each data point represents the average tumor volume of all mice in a given group. Data is representative of three independent experiments with 5-7 mice/group. (C) Shp1 protein relative to total Erk2 protein in peripheral blood cells from indicated mice 14 days after initial tamoxifen dose (200 mg/kg bid for 4 days) and 7 days after B16F10 tumor cells were implanted. Data is representative of at least three independent experiments with 5-7 mice per group. (D) Flow cytometric analysis of live CD45 + cells from E0771 tumors isolated from tamoxifen-treated Ptpn6 fl/fl and Ptpn6 fl/fl ERT2-cre mice at day 19 post tumor implantation. (E) E0771 tumor volume measurements in tamoxifen-treated Ptpn6 fl/fl ERT2-cre and Ptpn6 fl/fl mice. Each data point represents the average tumor volume of all mice in a given group. Data is representative of three independent experiments with 4-5 mice per group. Statistical significance was calculated at each time point using an unpaired t -test. (F) Shp1 protein relative to total Erk2 protein in peripheral blood cells from indicated mice 14 days after initial tamoxifen d
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- Figure 4. K14Cre;fl/fl mice develop esophageal fibrosis and ILD. (A) Masson-Trichrome staining of the lower esophagus of 3-mo-old fl/fl and K14Cre; fl/fl mice. (Bottom) Higher magnification images of the regions indicated by squares in the top panels. Bars: (top) 500 um; (bottom) 200 um. (B) The lower esophagi were cut along the longitudinal direction, spread on sheets, fixed in formalin, and embedded in the paraffin. Masson-Trichrome staining was performed, and the thickness of the lamina propria was evaluated at the ages of 1, 2, and 3 mo. Vertical bars represent lamina propria thickness. Bars, 50 um. (C) The thickness of lamina propria at upper (thoracic) esophagus was evaluated at the age of 3 mo. (D) The thickness of the circular muscle layer was evaluated in the sections of 3-mo-old mice. (E) Hydroxyproline contents of the lower esophagus were compared between these two strains of mice at the age of 3 mo. (F) mRNA levels of type I collagen, cytokines, chemokines, and growth factors in the lower esophagi of 3-mo-old mice were assessed. (G) The lower esophagus sections from 3-mo-old mice were immunohistochemically stained for IL-1beta. Bars, 50 um. (Right) The graph shows the result of semiquantitative scoring of the staining intensity. (H) H&E staining of the lungs from 1-, 2-, and 3-mo-old fl/fl and K14Cre;fl/fl mice. Arrows indicate the peribronchial lymphocyte aggregates. Bars, 200 um. (I) Infiltrating inflammatory cells were immunohistochemically stained for CD3 and
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- Figure 5. K14Cre;fl/fl mice exhibit prominent autoimmunity with Th2/Th17 cell polarization and abnormal B cell activation leading to autoantibody production. (A) A representative image of the gross appearance of spleens and inguinal lymph nodes from 3-mo-old fl/fl and K14Cre;fl/fl mice. (B) The number of splenocytes in 3-mo-old fl/fl and K14Cre;fl/fl mice was counted. n = 4 per each group. (C) Lymphocytes isolated from inguinal lymph nodes were stained for CD3 and CD4 and intracellularly stained for IL-4, IL-17A, and IFN-gamma. (Top) Representative two-dimensional plots for IL-4 and IL-17A in CD3 + CD4 + cells. (Bottom) The percentages of cells positive for IL-4, IL-17A, and IFN-gamma are summarized. n = 7 mice per each group. (D) Splenocytes from 3-mo-old fl/fl and K14Cre;fl/fl mice were stained for B220 and CD19. B220 + CD19 + cells were analyzed for CD19 expression. A representative histogram of mean fluorescence intensity (MFI; top) and the comparison of mean fluorescence intensities (bottom) are shown. n = 4 mice per genotype. (E) Sera from 3- and 8-mo-old mice were analyzed for the concentrations of total IgG, IgM, and IgA. n = 5-7 mice per genotype. Note that the results are shown with log scale. (F) Sera from 1-, 2-, 3-, and 8-mo-old mice were analyzed for the concentration of IL-6. n = 6-7 mice per genotype. (G) B cells were isolated from splenocytes of 3-mo-old mice and cultured for 48 h, either unstimulated or stimulated with anti-CD40 antibody (alphaCD40Ab). The c
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- Figure 4 Host humoral immune responses to vector delivery. ( a ) The titer of anti-adenoviral antibodies in mouse bronchoalveolar lavage fluid (BALF). The total anti-Ad antibodies (IgA, IgE, IgGs, IgM) were detected with ELISA in all groups. Data were presented as mean +- SD (standard deviation). ( b ) Neutralizing antibody in mouse BALF. n = 5, #: p < 0.05; **: p < 0.01. ( c ) B-cell presence in mouse lungs. B-cells were detected in mouse lungs with antibodies against B220 by immunohistochemistry staining. The positive cells are shown in brown color. ns, no significant difference.
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- Figure 1 Development of myeloid cell populations is not detectably altered in MphCK2alpha -/- mice. (A) Schematic of Csnk2a targeting vector. (B) Immunoblots of lysates from BMDMs, splenocytes, or peritoneal cavity cells of LysM cre or LysM cre CK2alpha fl/fl (MphCK2alpha - / - ) mice were probed for CK2alpha or beta-actin as a loading control. n = 2. (C) Splenic myeloid cell populations were stained and analyzed by flow cytometry. Plots depict gating for representative F4/80+ macrophages, Ly6C+ monocytes, and neutrophils (also Ly6G+). (D) Cells from spleen were stained for flow cytometry to enumerate immune cell populations. Data are from pooled experiments, n = 4-12 mice/group. (E) Cells from bone marrow were stained for flow cytometry to evaluate bone marrow precursor populations including common myeloid progenitors (CMPs), granulocyte-monocyte progenitors (GMPs), and megakaryocyte-erythrocyte progenitor (MEP). Cells were pre-gated on Lin-(B220, CD3, CD11b, GR-1 and TER-119), Sca-1-, cKit-. Density plots are representative from two experiments.
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- Figure 1. Steady-state Flt3-L BMDCs and splenic DCs display remarkably high levels of eIF2alpha without inhibition of translation. (A) Immunohistochemistry of mouse spleen with staining for CD11c (red) and p-eIF2alpha (green). Scale bar: 200 mum, magnification: 10x. Single color images are shown and merged picture (right row), high level of p-eIF2alpha staining is mostly found co-localizing in cells positive for CD11c+ (DCs, white arrowheads). (B) Immunohistochemistry of mouse spleen in the white pulp for CD11c (red), p-eIF2alpha (green), CD11b (blue), and B220 (turquoise). Scale bars: 50 mum, magnification: 40x. In the upper panel, magnified areas show p-eIF2 detection in cDC2 (CD11c+/CD11b+) and cDC1 (CD11c+/CD11b-). In the lower panel, magnified areas show p-eIF2 detection in pDCs (B220+/CD11c+) and in B cells (B220+ and CD11c-). (C) Relative p-eIF2alpha levels measured by flow in different mouse spleen populations. Statistical analysis was performed by Mann-Whitney test. ** P < 0.01. (D) Levels of p-eIF2alpha and total eIF2alpha were measured in DC populations by immunoblot. Sorted steady-state Flt3-L BMDCs were compared with MEFs and freshly isolated CD8alpha+ T cells stimulated or not with thapsigargin (Tg) for 2 h (200 nM). Ratio of p-eIF2alpha/eIF2alpha is quantified in the graph of the lower panel. (E) Levels of p-eIF2alpha and total eIF2alpha were measured in bulk Flt3-L BMDCs during different days of BM differentiation in vitro. (F) Levels of protein synthesis were
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- Fig 1 Agriculture (swine) exposure-related Organic Dust Extract (ODE) induced airway inflammation coupled with Collagen Induced Arthritis (CIA) model. ( A ) Line graph depicts mean with SE bars of arthritis inflammatory score at respective time points from treatment groups. Statistical difference versus sham denoted as ""a"" (p