Explore
Validate
Learn
Immunohistochemistry
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [14]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [3]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 14-0457-80 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD45RO Monoclonal Antibody (UCHL1), eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The UCHL1 monoclonal antibody reacts with human CD45RO, a 180 kDa isoform of CD45. CD45RO is expressed by most thymocytes, activated memory T cells, granulocytes and monocytes. CD22 is a ligand for CD45RO. Expression of CD45RO and CD45RA is used commonly to discriminate subsets of peripheral T cells. Applications Reported: The UCHL1 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. Applications Tested: The UCHL1 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- UCHL1
- Vial size
- 25 µg
- Concentration
- 0.5 mg/mL
- Storage
- 4° C
Submitted references Longitudinal analysis of subtype C envelope tropism for memory CD4(+) T cell subsets over the first 3 years of untreated HIV-1 infection.
Blocking the recruitment of naive CD4(+) T cells reverses immunosuppression in breast cancer.
Efficient generation of antigen-specific CTLs by the BAFF-activated human B Lymphocytes as APCs: a novel approach for immunotherapy.
TET-catalyzed oxidation of intragenic 5-methylcytosine regulates CTCF-dependent alternative splicing.
Aging influences the response of T cells to stimulation by the ellagitannin, oenothein B.
Defining CD4 T cell memory by the epigenetic landscape of CpG DNA methylation.
Functional expression of CD137 (4-1BB) on T helper follicular cells.
CD39 and CD161 modulate Th17 responses in Crohn's disease.
Prevailing role of contact guidance in intrastromal T-cell trapping in human pancreatic cancer.
Neutralizing IL-6 reduces human arterial allograft rejection by allowing emergence of CD161+ CD4+ regulatory T cells.
Characterization of human DNGR-1+ BDCA3+ leukocytes as putative equivalents of mouse CD8alpha+ dendritic cells.
Expression of endothelia and lymphocyte adhesion molecules in bronchus-associated lymphoid tissue (BALT) in adult human lung.
TCR-induced downregulation of protein tyrosine phosphatase PEST augments secondary T cell responses.
Interaction of human PD-L1 and B7-1.
Gartner MJ, Gorry PR, Tumpach C, Zhou J, Dantanarayana A, Chang JJ, Angelovich TA, Ellenberg P, Laumaea AE, Nonyane M, Moore PL, Lewin SR, Churchill MJ, Flynn JK, Roche M
Retrovirology 2020 Aug 6;17(1):24
Retrovirology 2020 Aug 6;17(1):24
Blocking the recruitment of naive CD4(+) T cells reverses immunosuppression in breast cancer.
Su S, Liao J, Liu J, Huang D, He C, Chen F, Yang L, Wu W, Chen J, Lin L, Zeng Y, Ouyang N, Cui X, Yao H, Su F, Huang JD, Lieberman J, Liu Q, Song E
Cell research 2017 Apr;27(4):461-482
Cell research 2017 Apr;27(4):461-482
Efficient generation of antigen-specific CTLs by the BAFF-activated human B Lymphocytes as APCs: a novel approach for immunotherapy.
Yiwen Z, Shilin G, Yingshi C, Lishi S, Baohong L, Chao L, Linghua L, Ting P, Hui Z
Oncotarget 2016 Nov 22;7(47):77732-77748
Oncotarget 2016 Nov 22;7(47):77732-77748
TET-catalyzed oxidation of intragenic 5-methylcytosine regulates CTCF-dependent alternative splicing.
Marina RJ, Sturgill D, Bailly MA, Thenoz M, Varma G, Prigge MF, Nanan KK, Shukla S, Haque N, Oberdoerffer S
The EMBO journal 2016 Feb 1;35(3):335-55
The EMBO journal 2016 Feb 1;35(3):335-55
Aging influences the response of T cells to stimulation by the ellagitannin, oenothein B.
Ramstead AG, Schepetkin IA, Todd K, Loeffelholz J, Berardinelli JG, Quinn MT, Jutila MA
International immunopharmacology 2015 Jun;26(2):367-77
International immunopharmacology 2015 Jun;26(2):367-77
Defining CD4 T cell memory by the epigenetic landscape of CpG DNA methylation.
Komori HK, Hart T, LaMere SA, Chew PV, Salomon DR
Journal of immunology (Baltimore, Md. : 1950) 2015 Feb 15;194(4):1565-79
Journal of immunology (Baltimore, Md. : 1950) 2015 Feb 15;194(4):1565-79
Functional expression of CD137 (4-1BB) on T helper follicular cells.
Alfaro C, Echeveste JI, Rodriguez-Ruiz ME, Solorzano JL, Perez-Gracia JL, Idoate MA, Lopez-Picazo JM, Sanchez-Paulete AR, Labiano S, Rouzaut A, Oñate C, Aznar A, Lozano MD, Melero I
Oncoimmunology 2015 Dec;4(12):e1054597
Oncoimmunology 2015 Dec;4(12):e1054597
CD39 and CD161 modulate Th17 responses in Crohn's disease.
Bai A, Moss A, Kokkotou E, Usheva A, Sun X, Cheifetz A, Zheng Y, Longhi MS, Gao W, Wu Y, Robson SC
Journal of immunology (Baltimore, Md. : 1950) 2014 Oct 1;193(7):3366-77
Journal of immunology (Baltimore, Md. : 1950) 2014 Oct 1;193(7):3366-77
Prevailing role of contact guidance in intrastromal T-cell trapping in human pancreatic cancer.
Hartmann N, Giese NA, Giese T, Poschke I, Offringa R, Werner J, Ryschich E
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Jul 1;20(13):3422-33
Clinical cancer research : an official journal of the American Association for Cancer Research 2014 Jul 1;20(13):3422-33
Neutralizing IL-6 reduces human arterial allograft rejection by allowing emergence of CD161+ CD4+ regulatory T cells.
Fogal B, Yi T, Wang C, Rao DA, Lebastchi A, Kulkarni S, Tellides G, Pober JS
Journal of immunology (Baltimore, Md. : 1950) 2011 Dec 15;187(12):6268-80
Journal of immunology (Baltimore, Md. : 1950) 2011 Dec 15;187(12):6268-80
Characterization of human DNGR-1+ BDCA3+ leukocytes as putative equivalents of mouse CD8alpha+ dendritic cells.
Poulin LF, Salio M, Griessinger E, Anjos-Afonso F, Craciun L, Chen JL, Keller AM, Joffre O, Zelenay S, Nye E, Le Moine A, Faure F, Donckier V, Sancho D, Cerundolo V, Bonnet D, Reis e Sousa C
The Journal of experimental medicine 2010 Jun 7;207(6):1261-71
The Journal of experimental medicine 2010 Jun 7;207(6):1261-71
Expression of endothelia and lymphocyte adhesion molecules in bronchus-associated lymphoid tissue (BALT) in adult human lung.
Kawamata N, Xu B, Nishijima H, Aoyama K, Kusumoto M, Takeuchi T, Tei C, Michie SA, Matsuyama T
Respiratory research 2009 Oct 22;10(1):97
Respiratory research 2009 Oct 22;10(1):97
TCR-induced downregulation of protein tyrosine phosphatase PEST augments secondary T cell responses.
Arimura Y, Vang T, Tautz L, Williams S, Mustelin T
Molecular immunology 2008 Jun;45(11):3074-84
Molecular immunology 2008 Jun;45(11):3074-84
Interaction of human PD-L1 and B7-1.
Butte MJ, Peña-Cruz V, Kim MJ, Freeman GJ, Sharpe AH
Molecular immunology 2008 Aug;45(13):3567-72
Molecular immunology 2008 Aug;45(13):3567-72
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with 0.25 µg of Mouse IgG2a kappa Isotype Control Purified (Product # 14-4724-82) (open histogram) or 0.25 µg of Anti-Human CD45RO Purified (filled histogram) followed by Anti-Mouse IgG FITC (Product # 11-4011-85). Cells in the lymphocyte gate were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 3 Transitional memory and effector memory cells were most frequently infected by C-HIV Envs. a Each data point represents the percentage of infected CD4 + T cells with one pseudovirus (averaged from four independent seronegative blood bank donors). The Env donor is indicated as follows; CAP88 (blue circles), CAP177 (red squares), CAP228 (green triangles), CAP255 (purple inverted triangles) and CAP257 (orange diamonds). Black lines represents the median of all pseudoviruses within each time point. Comparisons were made using a Kruskal-Wallis test with Dunn's post hoc test for multiple comparisons. b Stacked bar graphs represent the contribution of each T cell subset to the pool of infected CD4 + T cells. Values represent the median percentage of infected CD4 + T-cells (averaged across four HIV-seronegative PBMC donors) that belong to the indicated subset [naive; dark blue, T stem cell memory (TSCM); red, central memory (CM); yellow, transitional memory (TM); light blue, effector memory (EM); purple and terminally differentiated (TD); green], and are stratified by participant and time point. Error bars represent the interquartile range. c Dot plot representing the proportion of each T cell subset contributing to the total pool of infected cells for all Env-pseudoviruses. Each point represents a single virus averaged across four seronegative donors, lines represent median and error bars represent interquartile range. Comparisons were made using a Kruskal-Wallis test with Du
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 PITPNM3 is a CCL18 receptor on naive CD4 + T cells. (A) Representative flow cytometry staining for PITPNM3 and CCR8, potential CCL18 receptors, on gated PB T cell subsets and paired TI naive CD4 + T cells of a breast cancer patient. Cells were gated on CD3 + CD45RA + CD45RO - CD25 - CD4 + /CD8 + for naive CD4 + /CD8 + T cells, CD3 + CD45RA - CD45RO + CD25 - CD4 + /CD8 + for memory CD4 + /CD8 + T cells and CD3 + CD4 + CD25 + for Tregs). Quantitation of PITPNM3 and CCR8 expression on T cell subsets for eight breast cancer patients is provided in Supplementary information, Figure S8A . (B-F) Knockdown of PITPNM3 in naive CD4 + T cells inhibits CCL18 binding, signaling and chemotaxis. (B) Binding of 125 I-CCL18 to naive CD4 + T cells, knocked down or not for PITPNM3 (shPI-1,2) in the presence of increasing concentrations of unlabeled CCL18. Shown are the representative assays for three independent experiments using PB T cells from three normal donors. (C) Representative fluorescence microscopy images of CCL18 binding to naive CD4 + T cells, knocked down or not for PITPNM3 , stained for PITPNM3 and CCL18 3 h after adding CCL18. Scale bar, 5 mum. Shown are the representative images for three independent experiments using PB T cells from three normal donors. (D) Immunoblot of CCL18-treated naive CD4 + T cells, knocked down or not for PITPNM3 , showing expression of PITPNM3 and phosphorylated/total (t-) Erk1/2 and Akt, relative to GAPDH as a loading control. Blots are repres
