14-9458-82

antibody from Invitrogen Antibodies
Targeting: PTPRC CD45, GP180, LCA, T200
Provider product page for 14-9458-82

Antibody data

Product number
14-9458-82 - Provider product page
Provider
Invitrogen Antibodies
Product name
CD45RB Monoclonal Antibody (PD7/26), eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The monoclonal antibody PD7/26 recognizes CD45RB, which is an isoform of the Leukocyte Common Antigen (LCA) also known as CD45. CD45RB is a protein tyrosine phosphatase receptor that is present on most hematopoietic cells and absent on non-hematopoietic cells. Expression of CD45RB is more restricted than CD45 and can only be found on T cell subsets, B, NK, and myeloid cells. Regulatory T cells (Tregs) have been show to express low levels of CD45RB, which may correlate with increased migration to sites of infection. In contrast, high CD45RB expression results in IL-2 and IFN gamma production instead of IL-4 in vitro. Applications Reported: This PD7/26 antibody has been reported for use in Flow Cytometric Analysis, Western Blotting, Immunohistochemical Staining of Formalin-Fixed Paraffin Embedded Tissue Sections, and Immunocytochemistry. Applications Tested: This PD7/26 antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tonsil using low pH antigen retrieval. This can be used at less than or equal to 5 µg/mL. This PD7/26 antibody has also been tested by western blot at 5 µg/mL and and by flow cytometric analysis on human peripheral blood cells at 0.25 µg/test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
PD7/26
Vial size
100 µg
Concentration
0.5 mg/mL
Storage
4° C
PTPRC protein structure - 14-9458-82 shown in red.