Antibody data
- Antibody Data
- Antigen structure
- References [11]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [3]
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- Product number
- 25-0457-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD45RO Monoclonal Antibody (UCHL1), PE-Cyanine7, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The UCHL1 monoclonal antibody reacts with human CD45RO, a 180 kDa isoform of CD45. CD45RO is expressed by most thymocytes, activated memory T cells, granulocytes and monocytes. CD22 is a ligand for CD45RO. Expression of CD45RO and CD45RA is used commonly to discriminate subsets of peripheral T cells. Applications Reported: This UCHL1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This UCHL1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- UCHL1
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references CD8(+) T cells specific for an immunodominant SARS-CoV-2 nucleocapsid epitope display high naive precursor frequency and TCR promiscuity.
Development of CAR-T cell therapy for B-ALL using a point-of-care approach.
Longitudinal analysis of subtype C envelope tropism for memory CD4(+) T cell subsets over the first 3 years of untreated HIV-1 infection.
Single-Cell Approach to Influenza-Specific CD8(+) T Cell Receptor Repertoires Across Different Age Groups, Tissues, and Following Influenza Virus Infection.
Selective blockade of CD28 on human T cells facilitates regulation of alloimmune responses.
Blocking the recruitment of naive CD4(+) T cells reverses immunosuppression in breast cancer.
Modeling the dynamics of neonatal CD8(+) T-cell responses.
Promoter H3K4 methylation dynamically reinforces activation-induced pathways in human CD4 T cells.
Functional expression of CD137 (4-1BB) on T helper follicular cells.
Differential expression of CD300a/c on human TH1 and TH17 cells.
Human Th1 cells that express CD300a are polyfunctional and after stimulation up-regulate the T-box transcription factor eomesodermin.
Nguyen THO, Rowntree LC, Petersen J, Chua BY, Hensen L, Kedzierski L, van de Sandt CE, Chaurasia P, Tan HX, Habel JR, Zhang W, Allen LF, Earnest L, Mak KY, Juno JA, Wragg K, Mordant FL, Amanat F, Krammer F, Mifsud NA, Doolan DL, Flanagan KL, Sonda S, Kaur J, Wakim LM, Westall GP, James F, Mouhtouris E, Gordon CL, Holmes NE, Smibert OC, Trubiano JA, Cheng AC, Harcourt P, Clifton P, Crawford JC, Thomas PG, Wheatley AK, Kent SJ, Rossjohn J, Torresi J, Kedzierska K
Immunity 2021 May 11;54(5):1066-1082.e5
Immunity 2021 May 11;54(5):1066-1082.e5
Development of CAR-T cell therapy for B-ALL using a point-of-care approach.
de Macedo Abdo L, Barros LRC, Saldanha Viegas M, Vieira Codeço Marques L, de Sousa Ferreira P, Chicaybam L, Bonamino MH
Oncoimmunology 2020;9(1):1752592
Oncoimmunology 2020;9(1):1752592
Longitudinal analysis of subtype C envelope tropism for memory CD4(+) T cell subsets over the first 3 years of untreated HIV-1 infection.
Gartner MJ, Gorry PR, Tumpach C, Zhou J, Dantanarayana A, Chang JJ, Angelovich TA, Ellenberg P, Laumaea AE, Nonyane M, Moore PL, Lewin SR, Churchill MJ, Flynn JK, Roche M
Retrovirology 2020 Aug 6;17(1):24
Retrovirology 2020 Aug 6;17(1):24
Single-Cell Approach to Influenza-Specific CD8(+) T Cell Receptor Repertoires Across Different Age Groups, Tissues, and Following Influenza Virus Infection.
Sant S, Grzelak L, Wang Z, Pizzolla A, Koutsakos M, Crowe J, Loudovaris T, Mannering SI, Westall GP, Wakim LM, Rossjohn J, Gras S, Richards M, Xu J, Thomas PG, Loh L, Nguyen THO, Kedzierska K
Frontiers in immunology 2018;9:1453
Frontiers in immunology 2018;9:1453
Selective blockade of CD28 on human T cells facilitates regulation of alloimmune responses.
Zaitsu M, Issa F, Hester J, Vanhove B, Wood KJ
JCI insight 2017 Oct 5;2(19)
JCI insight 2017 Oct 5;2(19)
Blocking the recruitment of naive CD4(+) T cells reverses immunosuppression in breast cancer.
Su S, Liao J, Liu J, Huang D, He C, Chen F, Yang L, Wu W, Chen J, Lin L, Zeng Y, Ouyang N, Cui X, Yao H, Su F, Huang JD, Lieberman J, Liu Q, Song E
Cell research 2017 Apr;27(4):461-482
Cell research 2017 Apr;27(4):461-482
Modeling the dynamics of neonatal CD8(+) T-cell responses.
Reynaldi A, Smith NL, Schlub TE, Venturi V, Rudd BD, Davenport MP
Immunology and cell biology 2016 Oct;94(9):838-848
Immunology and cell biology 2016 Oct;94(9):838-848
Promoter H3K4 methylation dynamically reinforces activation-induced pathways in human CD4 T cells.
LaMere SA, Thompson RC, Komori HK, Mark A, Salomon DR
Genes and immunity 2016 Jul;17(5):283-97
Genes and immunity 2016 Jul;17(5):283-97
Functional expression of CD137 (4-1BB) on T helper follicular cells.
Alfaro C, Echeveste JI, Rodriguez-Ruiz ME, Solorzano JL, Perez-Gracia JL, Idoate MA, Lopez-Picazo JM, Sanchez-Paulete AR, Labiano S, Rouzaut A, Oñate C, Aznar A, Lozano MD, Melero I
Oncoimmunology 2015 Dec;4(12):e1054597
Oncoimmunology 2015 Dec;4(12):e1054597
Differential expression of CD300a/c on human TH1 and TH17 cells.
Simhadri VR, Mariano JL, Zhou Q, DeBell KE, Borrego F
BMC immunology 2011 Nov 2;12:62
BMC immunology 2011 Nov 2;12:62
Human Th1 cells that express CD300a are polyfunctional and after stimulation up-regulate the T-box transcription factor eomesodermin.
Narayanan S, Silva R, Peruzzi G, Alvarez Y, Simhadri VR, Debell K, Coligan JE, Borrego F
PloS one 2010 May 13;5(5):e10636
PloS one 2010 May 13;5(5):e10636
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Anti-Human CD45RA APC (Product # 17-0458-42) and Mouse IgG2a K Isotype Control PE-Cyanine7 (Product # 25-4724-81) (left) or Anti-Human CD45RO PE-Cyanine7 (right). Cells in the lymphocyte gate were used for analysis.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 3 Transitional memory and effector memory cells were most frequently infected by C-HIV Envs. a Each data point represents the percentage of infected CD4 + T cells with one pseudovirus (averaged from four independent seronegative blood bank donors). The Env donor is indicated as follows; CAP88 (blue circles), CAP177 (red squares), CAP228 (green triangles), CAP255 (purple inverted triangles) and CAP257 (orange diamonds). Black lines represents the median of all pseudoviruses within each time point. Comparisons were made using a Kruskal-Wallis test with Dunn's post hoc test for multiple comparisons. b Stacked bar graphs represent the contribution of each T cell subset to the pool of infected CD4 + T cells. Values represent the median percentage of infected CD4 + T-cells (averaged across four HIV-seronegative PBMC donors) that belong to the indicated subset [naive; dark blue, T stem cell memory (TSCM); red, central memory (CM); yellow, transitional memory (TM); light blue, effector memory (EM); purple and terminally differentiated (TD); green], and are stratified by participant and time point. Error bars represent the interquartile range. c Dot plot representing the proportion of each T cell subset contributing to the total pool of infected cells for all Env-pseudoviruses. Each point represents a single virus averaged across four seronegative donors, lines represent median and error bars represent interquartile range. Comparisons were made using a Kruskal-Wallis test with Du
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 5 PITPNM3 is a CCL18 receptor on naive CD4 + T cells. (A) Representative flow cytometry staining for PITPNM3 and CCR8, potential CCL18 receptors, on gated PB T cell subsets and paired TI naive CD4 + T cells of a breast cancer patient. Cells were gated on CD3 + CD45RA + CD45RO - CD25 - CD4 + /CD8 + for naive CD4 + /CD8 + T cells, CD3 + CD45RA - CD45RO + CD25 - CD4 + /CD8 + for memory CD4 + /CD8 + T cells and CD3 + CD4 + CD25 + for Tregs). Quantitation of PITPNM3 and CCR8 expression on T cell subsets for eight breast cancer patients is provided in Supplementary information, Figure S8A . (B-F) Knockdown of PITPNM3 in naive CD4 + T cells inhibits CCL18 binding, signaling and chemotaxis. (B) Binding of 125 I-CCL18 to naive CD4 + T cells, knocked down or not for PITPNM3 (shPI-1,2) in the presence of increasing concentrations of unlabeled CCL18. Shown are the representative assays for three independent experiments using PB T cells from three normal donors. (C) Representative fluorescence microscopy images of CCL18 binding to naive CD4 + T cells, knocked down or not for PITPNM3 , stained for PITPNM3 and CCL18 3 h after adding CCL18. Scale bar, 5 mum. Shown are the representative images for three independent experiments using PB T cells from three normal donors. (D) Immunoblot of CCL18-treated naive CD4 + T cells, knocked down or not for PITPNM3 , showing expression of PITPNM3 and phosphorylated/total (t-) Erk1/2 and Akt, relative to GAPDH as a loading control. Blots are repres