Antibody data
- Antibody Data
- Antigen structure
- References [27]
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- Validations
- Flow cytometry [1]
- Other assay [13]
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- Product number
- 45-9459-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD45 Monoclonal Antibody (2D1), PerCP-Cyanine5.5, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The 2D1 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes. Applications Reported: This 2D1 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 2D1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2D1
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Plasma Extracellular Vesicle Subtypes May be Useful as Potential Biomarkers of Immune Activation in People With HIV.
Bcl-xL mutant promotes cartilage differentiation of BMSCs by upregulating TGF-β/BMP expression levels.
N(6)-Methyladenosine on mRNA facilitates a phase-separated nuclear body that suppresses myeloid leukemic differentiation.
High Fructose Drives the Serine Synthesis Pathway in Acute Myeloid Leukemic Cells.
VAP-PLGA microspheres (VAP-PLGA) promote adipose-derived stem cells (ADSCs)-induced wound healing in chronic skin ulcers in mice via PI3K/Akt/HIF-1α pathway.
Down-Regulated Exosomal MicroRNA-221 - 3p Derived From Senescent Mesenchymal Stem Cells Impairs Heart Repair.
Single residue in CD28-costimulated CAR-T cells limits long-term persistence and antitumor durability.
TGF‑β induces periodontal ligament stem cell senescence through increase of ROS production.
Microglia innately develop within cerebral organoids.
Bone marrow niche trafficking of miR-126 controls the self-renewal of leukemia stem cells in chronic myelogenous leukemia.
A transcriptionally and functionally distinct PD-1(+) CD8(+) T cell pool with predictive potential in non-small-cell lung cancer treated with PD-1 blockade.
A fully defined static suspension culture system for large-scale human embryonic stem cell production.
Similarities and differences between helminth parasites and cancer cell lines in shaping human monocytes: Insights into parallel mechanisms of immune evasion.
Pancreatic cancer therapy with combined mesothelin-redirected chimeric antigen receptor T cells and cytokine-armed oncolytic adenoviruses.
Antibody-dependent fragmentation is a newly identified mechanism of cell killing in vivo.
The N(6)-methyladenosine (m(6)A)-forming enzyme METTL3 controls myeloid differentiation of normal hematopoietic and leukemia cells.
Increased expression of triggering receptor expressed on myeloid cells-1 in the population with obesity and insulin resistance.
The transcriptome of HIV-1 infected intestinal CD4+ T cells exposed to enteric bacteria.
Tumor immune microenvironment characterization in clear cell renal cell carcinoma identifies prognostic and immunotherapeutically relevant messenger RNA signatures.
Overexpression of TLR2 and TLR9 on monocyte subsets of active rheumatoid arthritis patients contributes to enhance responsiveness to TLR agonists.
Vascular niche promotes hematopoietic multipotent progenitor formation from pluripotent stem cells.
Progression of Lung Cancer Is Associated with Increased Dysfunction of T Cells Defined by Coexpression of Multiple Inhibitory Receptors.
Quantitative reconstruction of leukocyte subsets using DNA methylation.
Functional heterogeneity of genetically defined subclones in acute myeloid leukemia.
Epithelial cell differentiation of human mesenchymal stromal cells in decellularized lung scaffolds.
Neuregulin autocrine signaling promotes self-renewal of breast tumor-initiating cells by triggering HER2/HER3 activation.
Small molecule inhibitor of antigen binding and presentation by HLA-DR2b as a therapeutic strategy for the treatment of multiple sclerosis.
Bazié WW, Boucher J, Vitry J, Goyer B, Routy JP, Tremblay C, Trottier S, Jenabian MA, Provost P, Alary M, Gilbert C
Pathogens & immunity 2021;6(1):1-28
Pathogens & immunity 2021;6(1):1-28
Bcl-xL mutant promotes cartilage differentiation of BMSCs by upregulating TGF-β/BMP expression levels.
Xiao K, Yang L, Xie W, Gao X, Huang R, Xie M
Experimental and therapeutic medicine 2021 Jul;22(1):736
Experimental and therapeutic medicine 2021 Jul;22(1):736
N(6)-Methyladenosine on mRNA facilitates a phase-separated nuclear body that suppresses myeloid leukemic differentiation.
Cheng Y, Xie W, Pickering BF, Chu KL, Savino AM, Yang X, Luo H, Nguyen DT, Mo S, Barin E, Velleca A, Rohwetter TM, Patel DJ, Jaffrey SR, Kharas MG
Cancer cell 2021 Jul 12;39(7):958-972.e8
Cancer cell 2021 Jul 12;39(7):958-972.e8
High Fructose Drives the Serine Synthesis Pathway in Acute Myeloid Leukemic Cells.
Jeong S, Savino AM, Chirayil R, Barin E, Cheng Y, Park SM, Schurer A, Mullarky E, Cantley LC, Kharas MG, Keshari KR
Cell metabolism 2021 Jan 5;33(1):145-159.e6
Cell metabolism 2021 Jan 5;33(1):145-159.e6
VAP-PLGA microspheres (VAP-PLGA) promote adipose-derived stem cells (ADSCs)-induced wound healing in chronic skin ulcers in mice via PI3K/Akt/HIF-1α pathway.
Jiang W, Zhang J, Zhang X, Fan C, Huang J
Bioengineered 2021 Dec;12(2):10264-10284
Bioengineered 2021 Dec;12(2):10264-10284
Down-Regulated Exosomal MicroRNA-221 - 3p Derived From Senescent Mesenchymal Stem Cells Impairs Heart Repair.
Sun L, Zhu W, Zhao P, Zhang J, Lu Y, Zhu Y, Zhao W, Liu Y, Chen Q, Zhang F
Frontiers in cell and developmental biology 2020;8:263
Frontiers in cell and developmental biology 2020;8:263
Single residue in CD28-costimulated CAR-T cells limits long-term persistence and antitumor durability.
Guedan S, Madar A, Casado-Medrano V, Shaw C, Wing A, Liu F, Young RM, June CH, Posey AD Jr
The Journal of clinical investigation 2020 Jun 1;130(6):3087-3097
The Journal of clinical investigation 2020 Jun 1;130(6):3087-3097
TGF‑β induces periodontal ligament stem cell senescence through increase of ROS production.
Fan C, Ji Q, Zhang C, Xu S, Sun H, Li Z
Molecular medicine reports 2019 Oct;20(4):3123-3130
Molecular medicine reports 2019 Oct;20(4):3123-3130
Microglia innately develop within cerebral organoids.
Ormel PR, Vieira de Sá R, van Bodegraven EJ, Karst H, Harschnitz O, Sneeboer MAM, Johansen LE, van Dijk RE, Scheefhals N, Berdenis van Berlekom A, Ribes Martínez E, Kling S, MacGillavry HD, van den Berg LH, Kahn RS, Hol EM, de Witte LD, Pasterkamp RJ
Nature communications 2018 Oct 9;9(1):4167
Nature communications 2018 Oct 9;9(1):4167
Bone marrow niche trafficking of miR-126 controls the self-renewal of leukemia stem cells in chronic myelogenous leukemia.
Zhang B, Nguyen LXT, Li L, Zhao D, Kumar B, Wu H, Lin A, Pellicano F, Hopcroft L, Su YL, Copland M, Holyoake TL, Kuo CJ, Bhatia R, Snyder DS, Ali H, Stein AS, Brewer C, Wang H, McDonald T, Swiderski P, Troadec E, Chen CC, Dorrance A, Pullarkat V, Yuan YC, Perrotti D, Carlesso N, Forman SJ, Kortylewski M, Kuo YH, Marcucci G
Nature medicine 2018 May;24(4):450-462
Nature medicine 2018 May;24(4):450-462
A transcriptionally and functionally distinct PD-1(+) CD8(+) T cell pool with predictive potential in non-small-cell lung cancer treated with PD-1 blockade.
Thommen DS, Koelzer VH, Herzig P, Roller A, Trefny M, Dimeloe S, Kiialainen A, Hanhart J, Schill C, Hess C, Savic Prince S, Wiese M, Lardinois D, Ho PC, Klein C, Karanikas V, Mertz KD, Schumacher TN, Zippelius A
Nature medicine 2018 Jul;24(7):994-1004
Nature medicine 2018 Jul;24(7):994-1004
A fully defined static suspension culture system for large-scale human embryonic stem cell production.
Li X, Ma R, Gu Q, Liang L, Wang L, Zhang Y, Wang X, Liu X, Li Z, Fang J, Wu J, Wang Y, Li W, Hu B, Wang L, Zhou Q, Hao J
Cell death & disease 2018 Aug 30;9(9):892
Cell death & disease 2018 Aug 30;9(9):892
Similarities and differences between helminth parasites and cancer cell lines in shaping human monocytes: Insights into parallel mechanisms of immune evasion.
Narasimhan PB, Akabas L, Tariq S, Huda N, Bennuru S, Sabzevari H, Hofmeister R, Nutman TB, Tolouei Semnani R
PLoS neglected tropical diseases 2018 Apr;12(4):e0006404
PLoS neglected tropical diseases 2018 Apr;12(4):e0006404
Pancreatic cancer therapy with combined mesothelin-redirected chimeric antigen receptor T cells and cytokine-armed oncolytic adenoviruses.
Watanabe K, Luo Y, Da T, Guedan S, Ruella M, Scholler J, Keith B, Young RM, Engels B, Sorsa S, Siurala M, Havunen R, Tähtinen S, Hemminki A, June CH
JCI insight 2018 Apr 5;3(7)
JCI insight 2018 Apr 5;3(7)
Antibody-dependent fragmentation is a newly identified mechanism of cell killing in vivo.
Liew PX, Kim JH, Lee WY, Kubes P
Scientific reports 2017 Sep 5;7(1):10515
Scientific reports 2017 Sep 5;7(1):10515
The N(6)-methyladenosine (m(6)A)-forming enzyme METTL3 controls myeloid differentiation of normal hematopoietic and leukemia cells.
Vu LP, Pickering BF, Cheng Y, Zaccara S, Nguyen D, Minuesa G, Chou T, Chow A, Saletore Y, MacKay M, Schulman J, Famulare C, Patel M, Klimek VM, Garrett-Bakelman FE, Melnick A, Carroll M, Mason CE, Jaffrey SR, Kharas MG
Nature medicine 2017 Nov;23(11):1369-1376
Nature medicine 2017 Nov;23(11):1369-1376
Increased expression of triggering receptor expressed on myeloid cells-1 in the population with obesity and insulin resistance.
Subramanian S, Pallati PK, Rai V, Sharma P, Agrawal DK, Nandipati KC
Obesity (Silver Spring, Md.) 2017 Mar;25(3):527-538
Obesity (Silver Spring, Md.) 2017 Mar;25(3):527-538
The transcriptome of HIV-1 infected intestinal CD4+ T cells exposed to enteric bacteria.
Yoder AC, Guo K, Dillon SM, Phang T, Lee EJ, Harper MS, Helm K, Kappes JC, Ochsenbauer C, McCarter MD, Wilson CC, Santiago ML
PLoS pathogens 2017 Feb;13(2):e1006226
PLoS pathogens 2017 Feb;13(2):e1006226
Tumor immune microenvironment characterization in clear cell renal cell carcinoma identifies prognostic and immunotherapeutically relevant messenger RNA signatures.
Şenbabaoğlu Y, Gejman RS, Winer AG, Liu M, Van Allen EM, de Velasco G, Miao D, Ostrovnaya I, Drill E, Luna A, Weinhold N, Lee W, Manley BJ, Khalil DN, Kaffenberger SD, Chen Y, Danilova L, Voss MH, Coleman JA, Russo P, Reuter VE, Chan TA, Cheng EH, Scheinberg DA, Li MO, Choueiri TK, Hsieh JJ, Sander C, Hakimi AA
Genome biology 2016 Nov 17;17(1):231
Genome biology 2016 Nov 17;17(1):231
Overexpression of TLR2 and TLR9 on monocyte subsets of active rheumatoid arthritis patients contributes to enhance responsiveness to TLR agonists.
Lacerte P, Brunet A, Egarnes B, Duchêne B, Brown JP, Gosselin J
Arthritis research & therapy 2016 Jan 13;18:10
Arthritis research & therapy 2016 Jan 13;18:10
Vascular niche promotes hematopoietic multipotent progenitor formation from pluripotent stem cells.
Gori JL, Butler JM, Chan YY, Chandrasekaran D, Poulos MG, Ginsberg M, Nolan DJ, Elemento O, Wood BL, Adair JE, Rafii S, Kiem HP
The Journal of clinical investigation 2015 Mar 2;125(3):1243-54
The Journal of clinical investigation 2015 Mar 2;125(3):1243-54
Progression of Lung Cancer Is Associated with Increased Dysfunction of T Cells Defined by Coexpression of Multiple Inhibitory Receptors.
Thommen DS, Schreiner J, Müller P, Herzig P, Roller A, Belousov A, Umana P, Pisa P, Klein C, Bacac M, Fischer OS, Moersig W, Savic Prince S, Levitsky V, Karanikas V, Lardinois D, Zippelius A
Cancer immunology research 2015 Dec;3(12):1344-55
Cancer immunology research 2015 Dec;3(12):1344-55
Quantitative reconstruction of leukocyte subsets using DNA methylation.
Accomando WP, Wiencke JK, Houseman EA, Nelson HH, Kelsey KT
Genome biology 2014 Mar 5;15(3):R50
Genome biology 2014 Mar 5;15(3):R50
Functional heterogeneity of genetically defined subclones in acute myeloid leukemia.
Klco JM, Spencer DH, Miller CA, Griffith M, Lamprecht TL, O'Laughlin M, Fronick C, Magrini V, Demeter RT, Fulton RS, Eades WC, Link DC, Graubert TA, Walter MJ, Mardis ER, Dipersio JF, Wilson RK, Ley TJ
Cancer cell 2014 Mar 17;25(3):379-92
Cancer cell 2014 Mar 17;25(3):379-92
Epithelial cell differentiation of human mesenchymal stromal cells in decellularized lung scaffolds.
Mendez JJ, Ghaedi M, Steinbacher D, Niklason LE
Tissue engineering. Part A 2014 Jun;20(11-12):1735-46
Tissue engineering. Part A 2014 Jun;20(11-12):1735-46
Neuregulin autocrine signaling promotes self-renewal of breast tumor-initiating cells by triggering HER2/HER3 activation.
Lee CY, Lin Y, Bratman SV, Feng W, Kuo AH, Scheeren FA, Engreitz JM, Varma S, West RB, Diehn M
Cancer research 2014 Jan 1;74(1):341-52
Cancer research 2014 Jan 1;74(1):341-52
Small molecule inhibitor of antigen binding and presentation by HLA-DR2b as a therapeutic strategy for the treatment of multiple sclerosis.
Ji N, Somanaboeina A, Dixit A, Kawamura K, Hayward NJ, Self C, Olson GL, Forsthuber TG
Journal of immunology (Baltimore, Md. : 1950) 2013 Nov 15;191(10):5074-84
Journal of immunology (Baltimore, Md. : 1950) 2013 Nov 15;191(10):5074-84
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PerCP-Cyanine5-5 (Product # 45-4714-82) (blue histogram) or Anti-Human CD45 PerCP-Cyanine5-5 (purple histogram). Cells in the lymphocyte gate were used for analysis.
Supportive validation
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- Fig. 2 In silico validation of the immune cell scoring method. a In silico validation of immune cell scores using simulated mixing proportions. RNA-Seq profiles of FACS-sorted NK cells, macrophages, CD 4 + and CD 8 + T cells, and non-immune CD 45 - cells were mixed with known proportions to obtain a ""clean"" mixture. Noise was added at varying SNRs. Mixing levels were then inferred by ssGSEA from the ""clean"" and noisy mixtures. The Spearman correlations between the simulated and inferred levels ( top panel ) and the bootstrap p values for these correlation values ( bottom panel ) are shown on the y-axes (Additional file 1 : Figure S18 and "" Methods "" for the calculation of the bootstrap p values). b Validation of IIS with methylation-based leukocyte fractions. Spearman correlations between the two orthogonal scores are shown on the x-axis for 13 tumor types. c Validation of TIS with TCR beta chain abundance. Both scores are computationally inferred from RNA-Seq data but employ different approaches to measure T cell levels. Spearman correlations are shown on the x-axis for 19 tumor types
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- Figure 2. EV quantification and cell origin in association with HIV. Purified plasma EVs fromuninfected (Control, n = 8) and HIV+ patients (n = 17) were stained with the lipophilic fluorescent tracer dye DiD to count total vesicles ( Figure 2A and B ), then labeled with antibodies directed against receptors CD45 ( Figure 2C and D ), CD4 ( Figure 2E and F ), and CD8 ( Figure 2 G and H ) to evaluate cell origin by cytofluorometry. Asterisks denote significant difference (** P
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- FIGURE 1 Characterization of young and aged MSCs and exosomes. (A) Surface marker profiling of young-MSCs and aged-MSCs. (B) SA-beta-Gal staining showed that senescence increased significantly in aged MSCs. (C) Representative immunoblot images and quantitative analysis of p21, p53, and p16 protein level in young and aged-MSCs. ( n = 3). (D) Quantitation of cell cycle phases by propidium iodide staining. ( n = 3). (E) The CCK-8 assay showed that aged MSCs grew more slowly than young MSCs. ( n = 6). (F) Young and aged exosomes were observed using TEM. (G) The exosome surface markers were analyzed by Western blot. (H) Nanoparticle tracking analysis was used to analyze the particle size and concentration of Young-Exo and Aged-Exo. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001; NS, not significant.
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- Figure 1 Culture and identification of bone marrow mesenchymal stem cells. (A) Light microscopy of BMSCs (scale bar=100 um for the left image and 200 um for the right image). (B) Percentage of CD34-, CD45-, CD73-, CD90- and CD105-positive BMSCs were detected by flow cytometry. BMSC, bone marrow mesenchymal stem cell.
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- Figure 1. Morphology and immune phenotype of adipose-derived stem cells (ADSCs) were identified by morphological observation and flow cytometry. (a) Morphology of the primary (P1) and third passage (P3) of ADSCs. Images were acquired at 200x magnification. (b) Immune phenotype of ADSCs. The average data from three independent experiments were shown as mean +- standard deviation
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- Fig. 4 oMG expressed microglia-characteristic cell surface markers and showed similar functional immune and phagocytic properties as adult MG. a Flow cytometric analyses of the expression pattern of microglial extracellular markers on CD11b+-gated oMG (oMG 1, 3, and 5) compared to adult MG derived from three separate brain regions from adult MG1.1. (eight organoids were pooled per donor (oMG 1, 3, and 5) after 52 days in culture). b Morphology of magnetic automated cell sorted CD11b+ oMG 1 and adult MG in bright field microscope after 1 week in culture. Scale bar 40 mum. c mRNA expression, determined by qRT-PCR, of pro-inflammatory cytokines IL6 and IL1B after 6 h stimulation with LPS was significantly higher in oMG compared to adult MG (Mann-Whitney test IL6 and IL1B: U = 0, n = 4, p = 0.03). LPS-stimulated response relative to control condition without LPS. ( n = 4 experiments, eight organoids pooled per experiment; adult MG1.1) (* p < 0.05). d Anti-inflammatory response of oMG and adult MG was compared by qRT-PCR for expression of anti-inflammatory genes CD163 and MRC1 upon 72 h stimulation with dexamethasone. Dexamethasone-stimulated response relative to control condition without dexamethasone. (oMG, n = 3 separate experiments in which oMG were isolated from > 4 pooled cerebral organoids from iPSC 1 per experiment; adult MG, n = 4). e Phagocytosis capacity was tested oMG 1 and adult MG by performing a phagocytosis assay with iC3b-coated green-yellow
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- 01 Figure S1 Isotype control for TREM-1 and TREM-2 expression in monocytes and neutrophils of the study subjects. Cell phenotype was performed using anti-human antibodies and isotypes for monocytes (CD45+, CD14+, HLA-DR+) represented in figure A and neutrophils (CD45+, CD16+) in figure B. A) The representative figures for isotype control for monocytes gating; live monocytes were gated from FSC/SSC gating. Then CD45+ were gated using the basal level of CD45 Isotype and analyzed for CD14 and HLA-DR expression with respective CD14 and HLA-DR Isotypes. The double positive populations (CD14+ HLA-DR+) were further gated for expression of TREM-1+ and TREM-2+ using the basal level of TREM-1 and TREM-2 Isotypes. B) The representative figures for Isotype control for neutrophils gating; live granulocytes were gated from FSC/SSC gating. Then CD45+ were gated using the basal level of CD45 Isotype and analyzed for CD16 expression using the basal level of CD16 Isotype. CD16+ populations were further gated for expression of TREM-1 and TREM-2 using the basal level of TREM-1 and TREM-2 Isotypes. Figure S2 Hematoxylin and Eosin staining for fatty liver grading and inflammation. H & E in biopsy samples of SO - D - , SO + D - and SO + D + groups respectively in liver (images Aa, Ab & Ac), omental fat (images Ba, Bb & Bc) and subcutaneous fat (images Ca, Cb & Cc). Liver biopsy samples showed steatosis in SO + D - and fibrosis and cirrhosis in SO + D + groups. Size of the adipocyte was larger in su
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- Figure 1. Characterization of PDLSCs. (A) PDL cell clusters exhibited radiating or whirlpool-like morphology. The central structure in this image is a fragment of PDL tissue. Scale bar, 200 mum (B) CD146 + PDLSCs were small, round, fusiform and triangular. Scale bar, 100 mum. (C) PDLSCs were positive for the stem cell markers CD44, CD90 and CD105, but negative for CD34 and CD45, as detected by flow cytometry. PDL, periodontal ligament; PDLSCs, PDL stem cells.
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- Fig 3 Cancer cell lines and mf significantly upregulates the cell surface expressions of PDL1, PDL2, CD206 and VCAM-1 on human monocytes. Human monocytes were cultured in media alone, or with CMFDA-labeled three different cancer cell lines (MDA, OVCAR, U87), or with live mf of Brugia malayi for 48hr. Cells were harvested and cell surface expression PDL1, PDL2, CD206, VCAM-1, and CD163 was measured using flow cytometry gated on CD45 + /CMFDA - monocytes. (A) One representative set (n = 15) of flow histograms demonstrating cell surface expression in unexposed human monocytes and after exposure to mf or different cancer cell lines. (B). The data are expressed as the geometric mean with 95% confidence interval of the mean fluorescent intensity of unexposed and exposed monocytes ( n = 15). * P< 0.05, ** P< 0.005.
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- Fig. 4 Comparison of MSCs derived from 2D-hESCs and 3D-hESCs. a The morphology of 2D- and 3D-hESC-MSCs at different stages of differentiation. b Flow cytometry analysis revealed specific MSC surface markers (CD44, CD29, and CD105) with negative controls (CD34, CD19, and CD45) in 2D- and 3D-hESC-MSCs. c Immunostaining of differentiated 3D-hESC-MSCs expressing an adipocyte marker (FABP-4), osteocytes maker (osteocalcin), and chondrocytes marker (aggrecan)