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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- 50-9458-82 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD45RB Monoclonal Antibody (PD7/26), eFluor™ 660, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The monoclonal antibody PD7/26 recognizes CD45RB, which is an isoform of the Leukocyte Common Antigen (LCA) also known as CD45. CD45RB is a protein tyrosine phosphatase receptor that is present on most hematopoietic cells and absent on non-hematopoietic cells. Expression of CD45RB is more restricted than CD45 and can only be found on T cell subsets, B, NK, and myeloid cells. Regulatory T cells (Tregs) have been show to express low levels of CD45RB, which may correlate with increased migration to sites of infection. In contrast, high CD45RB expression results in IL-2 and IFN gamma production instead of IL-4 in vitro.
- Antibody clone number
- PD7/26
- Concentration
- 0.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry on formalin-fixed paraffin embedded human tonsil, using 10 µg/mL of Mouse IgG1 eFluor® 660 Isotype Control (left) or 10 µg/mL Anti-Human CD45RB eFluor® 660 (right). Nuclei are stained with DAPI.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of human peripheral blood mononuclear cells with CD45 Pacific Orange, CD45RA Pacific Blue, CD45RO FITC, and CD45RB eFluor 660. As expected based on known relative expression patterns, CD45RB clone PD7/26 stains a subset of lymphocytes (pink) and monocytes (orange), but not granulocytes (blue).
