Antibody data
- Antibody Data
- Antigen structure
- References [18]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 61-0459-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD45 Monoclonal Antibody (HI30), PE-eFluor 610, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The HI30 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes. Applications Reported: This HI30 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HI30 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome. Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- HI30
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Tumor-associated B cells and humoral immune response in head and neck squamous cell carcinoma.
Mechanisms of virus dissemination in bone marrow of HIV-1-infected humanized BLT mice.
Redundant and receptor-specific activities of TRADD, RIPK1 and FADD in death receptor signaling.
Single nucleotide variant profiles of viable single circulating tumour cells reveal CTC behaviours in breast cancer.
Helicase-Driven Activation of NFκB-COX2 Pathway Mediates the Immunosuppressive Component of dsRNA-Driven Inflammation in the Human Tumor Microenvironment.
Targeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis.
Preferential accumulation of regulatory T cells with highly immunosuppressive characteristics in breast tumor microenvironment.
Evaluation of a simple method for storage of blood samples that enables isolation of circulating tumor cells 96 h after sample collection.
Kinase-independent function of RIP1, critical for mature T-cell survival and proliferation.
Platelets confound the measurement of extracellular miRNA in archived plasma.
Targeting methyltransferase PRMT5 eliminates leukemia stem cells in chronic myelogenous leukemia.
UTX inhibition as selective epigenetic therapy against TAL1-driven T-cell acute lymphoblastic leukemia.
Circulating tumor cell detection in hepatocellular carcinoma based on karyoplasmic ratios using imaging flow cytometry.
Human esophageal myofibroblasts secrete proinflammatory cytokines in response to acid and Toll-like receptor 4 ligands.
Retinoic acid regulates hematopoietic development from human pluripotent stem cells.
Hypoxia-cultured human adipose-derived mesenchymal stem cells are non-oncogenic and have enhanced viability, motility, and tropism to brain cancer.
Microvesicles derived from human umbilical cord Wharton's jelly mesenchymal stem cells attenuate bladder tumor cell growth in vitro and in vivo.
Yield and characterization of subcutaneous human adipose-derived stem cells by flow cytometric and adipogenic mRNA analyzes.
Lechner A, Schlößer HA, Thelen M, Wennhold K, Rothschild SI, Gilles R, Quaas A, Siefer OG, Huebbers CU, Cukuroglu E, Göke J, Hillmer A, Gathof B, Meyer MF, Klussmann JP, Shimabukuro-Vornhagen A, Theurich S, Beutner D, von Bergwelt-Baildon M
Oncoimmunology 2019;8(3):1535293
Oncoimmunology 2019;8(3):1535293
Mechanisms of virus dissemination in bone marrow of HIV-1-infected humanized BLT mice.
Ladinsky MS, Khamaikawin W, Jung Y, Lin S, Lam J, An DS, Bjorkman PJ, Kieffer C
eLife 2019 Oct 28;8
eLife 2019 Oct 28;8
Redundant and receptor-specific activities of TRADD, RIPK1 and FADD in death receptor signaling.
Füllsack S, Rosenthal A, Wajant H, Siegmund D
Cell death & disease 2019 Feb 11;10(2):122
Cell death & disease 2019 Feb 11;10(2):122
Single nucleotide variant profiles of viable single circulating tumour cells reveal CTC behaviours in breast cancer.
Wang Y, Guo L, Feng L, Zhang W, Xiao T, Di X, Chen G, Zhang K
Oncology reports 2018 May;39(5):2147-2159
Oncology reports 2018 May;39(5):2147-2159
Helicase-Driven Activation of NFκB-COX2 Pathway Mediates the Immunosuppressive Component of dsRNA-Driven Inflammation in the Human Tumor Microenvironment.
Theodoraki MN, Yerneni S, Sarkar SN, Orr B, Muthuswamy R, Voyten J, Modugno F, Jiang W, Grimm M, Basse PH, Bartlett DL, Edwards RP, Kalinski P
Cancer research 2018 Aug 1;78(15):4292-4302
Cancer research 2018 Aug 1;78(15):4292-4302
Targeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis.
Li Y, Brauer PM, Singh J, Xhiku S, Yoganathan K, Zúñiga-Pflücker JC, Anderson MK
Stem cell reports 2017 Sep 12;9(3):779-795
Stem cell reports 2017 Sep 12;9(3):779-795
Preferential accumulation of regulatory T cells with highly immunosuppressive characteristics in breast tumor microenvironment.
Syed Khaja AS, Toor SM, El Salhat H, Faour I, Ul Haq N, Ali BR, Elkord E
Oncotarget 2017 May 16;8(20):33159-33171
Oncotarget 2017 May 16;8(20):33159-33171
Evaluation of a simple method for storage of blood samples that enables isolation of circulating tumor cells 96 h after sample collection.
Apostolou P, Ntanovasilis DA, Papasotiriou I
Journal of biological research (Thessalonike, Greece) 2017 Dec;24:11
Journal of biological research (Thessalonike, Greece) 2017 Dec;24:11
Kinase-independent function of RIP1, critical for mature T-cell survival and proliferation.
Dowling JP, Cai Y, Bertin J, Gough PJ, Zhang J
Cell death & disease 2016 Sep 29;7(9):e2379
Cell death & disease 2016 Sep 29;7(9):e2379
Platelets confound the measurement of extracellular miRNA in archived plasma.
Mitchell AJ, Gray WD, Hayek SS, Ko YA, Thomas S, Rooney K, Awad M, Roback JD, Quyyumi A, Searles CD
Scientific reports 2016 Sep 13;6:32651
Scientific reports 2016 Sep 13;6:32651
Targeting methyltransferase PRMT5 eliminates leukemia stem cells in chronic myelogenous leukemia.
Jin Y, Zhou J, Xu F, Jin B, Cui L, Wang Y, Du X, Li J, Li P, Ren R, Pan J
The Journal of clinical investigation 2016 Oct 3;126(10):3961-3980
The Journal of clinical investigation 2016 Oct 3;126(10):3961-3980
UTX inhibition as selective epigenetic therapy against TAL1-driven T-cell acute lymphoblastic leukemia.
Benyoucef A, Palii CG, Wang C, Porter CJ, Chu A, Dai F, Tremblay V, Rakopoulos P, Singh K, Huang S, Pflumio F, Hébert J, Couture JF, Perkins TJ, Ge K, Dilworth FJ, Brand M
Genes & development 2016 Mar 1;30(5):508-21
Genes & development 2016 Mar 1;30(5):508-21
Circulating tumor cell detection in hepatocellular carcinoma based on karyoplasmic ratios using imaging flow cytometry.
Liu Z, Guo W, Zhang D, Pang Y, Shi J, Wan S, Cheng K, Wang J, Cheng S
Scientific reports 2016 Dec 23;6:39808
Scientific reports 2016 Dec 23;6:39808
Human esophageal myofibroblasts secrete proinflammatory cytokines in response to acid and Toll-like receptor 4 ligands.
Gargus M, Niu C, Vallone JG, Binkley J, Rubin DC, Shaker A
American journal of physiology. Gastrointestinal and liver physiology 2015 Jun 1;308(11):G904-23
American journal of physiology. Gastrointestinal and liver physiology 2015 Jun 1;308(11):G904-23
Retinoic acid regulates hematopoietic development from human pluripotent stem cells.
Rönn RE, Guibentif C, Moraghebi R, Chaves P, Saxena S, Garcia B, Woods NB
Stem cell reports 2015 Feb 10;4(2):269-81
Stem cell reports 2015 Feb 10;4(2):269-81
Hypoxia-cultured human adipose-derived mesenchymal stem cells are non-oncogenic and have enhanced viability, motility, and tropism to brain cancer.
Feng Y, Zhu M, Dangelmajer S, Lee YM, Wijesekera O, Castellanos CX, Denduluri A, Chaichana KL, Li Q, Zhang H, Levchenko A, Guerrero-Cazares H, Quiñones-Hinojosa A
Cell death & disease 2014 Dec 11;5:e1567
Cell death & disease 2014 Dec 11;5:e1567
Microvesicles derived from human umbilical cord Wharton's jelly mesenchymal stem cells attenuate bladder tumor cell growth in vitro and in vivo.
Wu S, Ju GQ, Du T, Zhu YJ, Liu GH
PloS one 2013;8(4):e61366
PloS one 2013;8(4):e61366
Yield and characterization of subcutaneous human adipose-derived stem cells by flow cytometric and adipogenic mRNA analyzes.
Yu G, Wu X, Dietrich MA, Polk P, Scott LK, Ptitsyn AA, Gimble JM
Cytotherapy 2010 Jul;12(4):538-46
Cytotherapy 2010 Jul;12(4):538-46
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE-eFluor® 610 (Product # 61-4714-82) (blue histogram) or Anti-Human CD45 PE-eFluor® 610 (purple histogram). Cells in the lymphocyte gate were used for analysis.