Antibody data
- Antibody Data
- Antigen structure
- References [18]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 69-0459-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD45 Monoclonal Antibody (HI30), eFluor 506, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The HI30 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes. Applications Reported: This HI30 antibody has been reported for use in flow cytometric analysis. Applications Tested: This HI30 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 506 can be excited with the violet laser line (405 nm) and emits at 506 nm. We recommend using a 510/20 band pass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome. Excitation: 405 nm; Emission: 506 nm; Laser: Violet Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- HI30
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Mechanisms of virus dissemination in bone marrow of HIV-1-infected humanized BLT mice.
Single nucleotide variant profiles of viable single circulating tumour cells reveal CTC behaviours in breast cancer.
Targeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis.
Preferential accumulation of regulatory T cells with highly immunosuppressive characteristics in breast tumor microenvironment.
Evaluation of a simple method for storage of blood samples that enables isolation of circulating tumor cells 96 h after sample collection.
Kinase-independent function of RIP1, critical for mature T-cell survival and proliferation.
Platelets confound the measurement of extracellular miRNA in archived plasma.
Targeting methyltransferase PRMT5 eliminates leukemia stem cells in chronic myelogenous leukemia.
A 3D culture system enhances the ability of human bone marrow stromal cells to support the growth of limbal stem/progenitor cells.
UTX inhibition as selective epigenetic therapy against TAL1-driven T-cell acute lymphoblastic leukemia.
Circulating tumor cell detection in hepatocellular carcinoma based on karyoplasmic ratios using imaging flow cytometry.
Human esophageal myofibroblasts secrete proinflammatory cytokines in response to acid and Toll-like receptor 4 ligands.
Retinoic acid regulates hematopoietic development from human pluripotent stem cells.
Caveolin-1 regulates chemokine receptor 5-mediated contribution of bone marrow-derived cells to dermal fibrosis.
A Small Molecule β2 Integrin Agonist Improves Chronic Kidney Allograft Survival by Reducing Leukocyte Recruitment and Accompanying Vasculopathy.
Hypoxia-cultured human adipose-derived mesenchymal stem cells are non-oncogenic and have enhanced viability, motility, and tropism to brain cancer.
Microvesicles derived from human umbilical cord Wharton's jelly mesenchymal stem cells attenuate bladder tumor cell growth in vitro and in vivo.
Yield and characterization of subcutaneous human adipose-derived stem cells by flow cytometric and adipogenic mRNA analyzes.
Ladinsky MS, Khamaikawin W, Jung Y, Lin S, Lam J, An DS, Bjorkman PJ, Kieffer C
eLife 2019 Oct 28;8
eLife 2019 Oct 28;8
Single nucleotide variant profiles of viable single circulating tumour cells reveal CTC behaviours in breast cancer.
Wang Y, Guo L, Feng L, Zhang W, Xiao T, Di X, Chen G, Zhang K
Oncology reports 2018 May;39(5):2147-2159
Oncology reports 2018 May;39(5):2147-2159
Targeted Disruption of TCF12 Reveals HEB as Essential in Human Mesodermal Specification and Hematopoiesis.
Li Y, Brauer PM, Singh J, Xhiku S, Yoganathan K, Zúñiga-Pflücker JC, Anderson MK
Stem cell reports 2017 Sep 12;9(3):779-795
Stem cell reports 2017 Sep 12;9(3):779-795
Preferential accumulation of regulatory T cells with highly immunosuppressive characteristics in breast tumor microenvironment.
Syed Khaja AS, Toor SM, El Salhat H, Faour I, Ul Haq N, Ali BR, Elkord E
Oncotarget 2017 May 16;8(20):33159-33171
Oncotarget 2017 May 16;8(20):33159-33171
Evaluation of a simple method for storage of blood samples that enables isolation of circulating tumor cells 96 h after sample collection.
Apostolou P, Ntanovasilis DA, Papasotiriou I
Journal of biological research (Thessalonike, Greece) 2017 Dec;24:11
Journal of biological research (Thessalonike, Greece) 2017 Dec;24:11
Kinase-independent function of RIP1, critical for mature T-cell survival and proliferation.
Dowling JP, Cai Y, Bertin J, Gough PJ, Zhang J
Cell death & disease 2016 Sep 29;7(9):e2379
Cell death & disease 2016 Sep 29;7(9):e2379
Platelets confound the measurement of extracellular miRNA in archived plasma.
Mitchell AJ, Gray WD, Hayek SS, Ko YA, Thomas S, Rooney K, Awad M, Roback JD, Quyyumi A, Searles CD
Scientific reports 2016 Sep 13;6:32651
Scientific reports 2016 Sep 13;6:32651
Targeting methyltransferase PRMT5 eliminates leukemia stem cells in chronic myelogenous leukemia.
Jin Y, Zhou J, Xu F, Jin B, Cui L, Wang Y, Du X, Li J, Li P, Ren R, Pan J
The Journal of clinical investigation 2016 Oct 3;126(10):3961-3980
The Journal of clinical investigation 2016 Oct 3;126(10):3961-3980
A 3D culture system enhances the ability of human bone marrow stromal cells to support the growth of limbal stem/progenitor cells.
González S, Mei H, Nakatsu MN, Baclagon ER, Deng SX
Stem cell research 2016 Mar;16(2):358-64
Stem cell research 2016 Mar;16(2):358-64
UTX inhibition as selective epigenetic therapy against TAL1-driven T-cell acute lymphoblastic leukemia.
Benyoucef A, Palii CG, Wang C, Porter CJ, Chu A, Dai F, Tremblay V, Rakopoulos P, Singh K, Huang S, Pflumio F, Hébert J, Couture JF, Perkins TJ, Ge K, Dilworth FJ, Brand M
Genes & development 2016 Mar 1;30(5):508-21
Genes & development 2016 Mar 1;30(5):508-21
Circulating tumor cell detection in hepatocellular carcinoma based on karyoplasmic ratios using imaging flow cytometry.
Liu Z, Guo W, Zhang D, Pang Y, Shi J, Wan S, Cheng K, Wang J, Cheng S
Scientific reports 2016 Dec 23;6:39808
Scientific reports 2016 Dec 23;6:39808
Human esophageal myofibroblasts secrete proinflammatory cytokines in response to acid and Toll-like receptor 4 ligands.
Gargus M, Niu C, Vallone JG, Binkley J, Rubin DC, Shaker A
American journal of physiology. Gastrointestinal and liver physiology 2015 Jun 1;308(11):G904-23
American journal of physiology. Gastrointestinal and liver physiology 2015 Jun 1;308(11):G904-23
Retinoic acid regulates hematopoietic development from human pluripotent stem cells.
Rönn RE, Guibentif C, Moraghebi R, Chaves P, Saxena S, Garcia B, Woods NB
Stem cell reports 2015 Feb 10;4(2):269-81
Stem cell reports 2015 Feb 10;4(2):269-81
Caveolin-1 regulates chemokine receptor 5-mediated contribution of bone marrow-derived cells to dermal fibrosis.
Lee R, Perry B, Heywood J, Reese C, Bonner M, Hatfield CM, Silver RM, Visconti RP, Hoffman S, Tourkina E
Frontiers in pharmacology 2014;5:140
Frontiers in pharmacology 2014;5:140
A Small Molecule β2 Integrin Agonist Improves Chronic Kidney Allograft Survival by Reducing Leukocyte Recruitment and Accompanying Vasculopathy.
Khan SQ, Guo L, Cimbaluk DJ, Elshabrawy H, Faridi MH, Jolly M, George JF, Agarwal A, Gupta V
Frontiers in medicine 2014;1:45
Frontiers in medicine 2014;1:45
Hypoxia-cultured human adipose-derived mesenchymal stem cells are non-oncogenic and have enhanced viability, motility, and tropism to brain cancer.
Feng Y, Zhu M, Dangelmajer S, Lee YM, Wijesekera O, Castellanos CX, Denduluri A, Chaichana KL, Li Q, Zhang H, Levchenko A, Guerrero-Cazares H, Quiñones-Hinojosa A
Cell death & disease 2014 Dec 11;5:e1567
Cell death & disease 2014 Dec 11;5:e1567
Microvesicles derived from human umbilical cord Wharton's jelly mesenchymal stem cells attenuate bladder tumor cell growth in vitro and in vivo.
Wu S, Ju GQ, Du T, Zhu YJ, Liu GH
PloS one 2013;8(4):e61366
PloS one 2013;8(4):e61366
Yield and characterization of subcutaneous human adipose-derived stem cells by flow cytometric and adipogenic mRNA analyzes.
Yu G, Wu X, Dietrich MA, Polk P, Scott LK, Ptitsyn AA, Gimble JM
Cytotherapy 2010 Jul;12(4):538-46
Cytotherapy 2010 Jul;12(4):538-46
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control eFluor® 506 (Product # 69-4714-82) (blue histogram) or Anti-Human CD45 eFluor® 506 (purple histogram). Cells in the lymphocyte gate were used for analysis.