Antibody data

Product number
42-0452-82 - Provider product page
Provider
Invitrogen Antibodies
Product name
CD45R (B220) Monoclonal Antibody (RA3-6B2), eFluor™ 615, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen. Applications Reported: This RA3-6B2 antibody has been reported for use in immunohistochemical staining of frozen tissue sections (IHC-F), and immunocytochemistry. Applications Tested: This RA3-6B2 antibody has been tested by immunohistochemistry on frozen mouse spleen at less than or equal to 1 µg/mL. It is recommended that this antibody be carefully titrated for optimal performance in the assay of interest. This product has not been validated for flow cytometric analysis. Filter Recommendation: When using this eFluor® 615 antibody conjugate, we recommend a filter that will capture the 615 emission wavelength (for example, Excitation 560/55, 585LP, Emission 645/75). A standard Alexa Fluor® 594 filter is acceptable. Excitation: 595 nm; Emission: 615 nm. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human, Mouse
Host
Rat
Isotype
IgG
Antibody clone number
RA3-6B2
Vial size
100 µg
Concentration
0.2 mg/mL
Storage
4° C, store in dark, DO NOT FREEZE!
PTPRC protein structure - 42-0452-82 shown in red.