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Immunoprecipitation
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [5]
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- Product number
- NB100-77420SS - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal B220/CD45R Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified.
- Reactivity
- Human, Mouse, Feline
- Host
- Rat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: B220/CD45R Antibody (RA3-6B2) [NB100-77420] - Analysis using the Cy5 conjugate of NB100-77420. Multiple staining of BALB/c splenocytes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: B220/CD45R Antibody (RA3-6B2) [NB100-77420] - Analysis of Biotin conjugate of NB100-77420. Staining of BALB/c splenocytes with 0.06 ug of Rat IgG2a kappa Isotype Control Biotin (open histogram) or 0.06 ug of Anti-Human/Mouse CD45R (B220) Biotin (filled histogram) followed by Streptavidin PE.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: B220/CD45R Antibody (RA3-6B2) [NB100-77420] - C57BL/6 mouse splenocytes stained with purified RA3-6B2, followed by anti-rat IgG FITC
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Cell Surface): B220/CD45R Antibody (RA3-6B2) [NB100-77420] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-77420. Staining of CD45R on 10^6 BALB/c mouse splenocytes using 0.25 ug of this antibody. Green histogram represents isotype control; red represents CD45R antibody. Cell surface flow kit was used for this test (cells were not fixed for testing).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Cell Surface): B220/CD45R Antibody (RA3-6B2) [NB100-77420] - Analysis using the Alexa Fluor (R) 647 conjugate of NB100-77420. Staining of CD45R on 10^6 BALB/c mouse splenocytes using 0.25 ug of this antibody. Green histogram represents isotype control; red represents CD45R antibody. Cell surface flow kit was used for this test (cells were not fixed for testing).
