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Immunoprecipitation
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [3]
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- Product number
- NB100-65018-0.1mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal B220/CD45R Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. This antibody recognizes canine CD45R and was clustered as a canine CD45R at the first canine Leucocyte Antigen workshop. It precipitates an antigen of 220kD from Con A Blasts (1) and may be against a CD45R isoform. It is expressed on subsets of leucocytes (neutrophils are weak) in canine blood of all out bred mongrels and beagles tested.
- Reactivity
- Canine
- Host
- Rat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: B220/CD45R Antibody (YKIX753.22.2) [NB100-65018] - Figure A. Alexa Fluor 488 conjugated Rat anti Canine CD44 and RPE conjugated Rat IgG2a isotype control. Figure B. Alexa Fluor 488 conjugated Rat anti Canine CD44 and RPE conjugated Rat anti Canine CD45R.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: B220/CD45R Antibody (YKIX753.22.2) [NB100-65018] - Figure A. Alexa Fluor 488 conjugated Rat anti Dog CD44 and Alexa Fluor 647 conjugated Rat IgG2b isotype control. Figure B. Alexa Fluor 488 conjugated Rat anti Dog CD44 and Alexa Fluor 647 conjugated Rat anti Dog CD45R.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: B220/CD45R Antibody (YKIX753.22.2) [NB100-65018] - Figure A. RPE conjugated Rat anti Canine CD45R and Alexa Fluor 488 conjugated Rat IgG1 isotype control. Figure B. RPE conjugated Mouse anti Canine CD45R and Alexa Fluor 488 conjugated Rat anti Canine CD44.
