PA5-87427

antibody from Invitrogen Antibodies

Targeting: PTPRC CD45, GP180, LCA, T200

Western blot

ELISA

Antibody data

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Validation data

Product number: PA5-87427 - Provider product page
Provider: Invitrogen Antibodies
Product name: CD45 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Positive Samples: THP-1; Cellular Location: Membrane, Membrane raft, Single-pass type I membrane protein Immunogen sequence: NLDEQQELVE RDDEKQLMNV EPIHADILLE TYKRKIADEG RLFLAEFQSI PRVFSKFPIK EARKPFNQNK NRYVDILPYD YNRVELSEIN GDAGSNYINA SYIDGFKEPR KYIAAQGPRD
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 μL
Concentration: 1.1 mg/mL
Storage: -20°C, Avoid Freeze/Thaw Cycles

PTPRC protein structure - PA5-87427 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of Jurkat cells using PTPRC Polyclonal antibody (Product # PA5-87427) at dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of CD45 was performed in Jurkat cells using CD45 Polyclonal Antibody (Product # PA5-87427) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of CD45 was performed in Jurkat cells using CD45 Polyclonal Antibody (Product # PA5-87427) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of CD45 was performed in RAW264.7 cells using CD45 Polyclonal Antibody (Product # PA5-87427) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of CD45 in Jurkat cells. Samples were incubated with CD45 Polyclonal antibody (Product # PA5-87427) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of CD45 in rat spleen cells. Samples were incubated with CD45 Polyclonal antibody (Product # PA5-87427) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescence analysis of CD45 in mouse spleen cells. Samples were incubated with CD45 Polyclonal antibody (Product # PA5-87427) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.