- MA5-13197 - Invitrogen Antibodies PTPRC antibody

Submitted references High Therapeutic and Esthetic Properties of Extracellular Vesicles Produced from the Stem Cells and Their Spheroids Cultured from Ocular Surgery-Derived Waste Orbicularis Oculi Muscle Tissues.

Lim KM, Dayem AA, Choi Y, Lee Y, An J, Gil M, Lee S, Kwak HJ, Vellingirl B, Shin HJ, Cho SG
Antioxidants (Basel, Switzerland) 2021 Aug 16;10(8)

Immune Suppressive Effects of Plasma-Derived Exosome Populations in Head and Neck Cancer.

Beccard IJ, Hofmann L, Schroeder JC, Ludwig S, Laban S, Brunner C, Lotfi R, Hoffmann TK, Jackson EK, Schuler PJ, Theodoraki MN
Cancers 2020 Jul 21;12(7)

Human breast cancer biopsies induce eosinophil recruitment and enhance adjacent cancer cell proliferation.

Szalayova G, Ogrodnik A, Spencer B, Wade J, Bunn J, Ambaye A, James T, Rincon M
Breast cancer research and treatment 2016 Jun;157(3):461-74

Revisiting Cardiac Cellular Composition.

Pinto AR, Ilinykh A, Ivey MJ, Kuwabara JT, D'Antoni ML, Debuque R, Chandran A, Wang L, Arora K, Rosenthal NA, Tallquist MD
Circulation research 2016 Feb 5;118(3):400-9

An improved protocol for efficient engraftment in NOD/LTSZ-SCIDIL-2Rγnull mice allows HIV replication and development of anti-HIV immune responses.

Singh M, Singh P, Gaudray G, Musumeci L, Thielen C, Vaira D, Vandergeeten C, Delacroix L, Van Gulck E, Vanham G, de Leval L, Rahmouni S, Moutschen M
PloS one 2012;7(6):e38491

Differentiation of human mesenchymal stem cells: the potential mechanism for estrogen-induced preferential osteoblast versus adipocyte differentiation.

Zhao JW, Gao ZL, Mei H, Li YL, Wang Y
The American journal of the medical sciences 2011 Jun;341(6):460-8

Composite lymphoma in the anterior mediastinum: a case report and review of the literature.

Yu G, Kong L, Qu G, Zhang Q, Wang W, Jiang L
Diagnostic pathology 2011 Jul 6;6:60

Genetic predisposition directs breast cancer phenotype by dictating progenitor cell fate.

Proia TA, Keller PJ, Gupta PB, Klebba I, Jones AD, Sedic M, Gilmore H, Tung N, Naber SP, Schnitt S, Lander ES, Kuperwasser C
Cell stem cell 2011 Feb 4;8(2):149-63

A 19-year follow-up of a patient with type 3 ectrodactyly-ectodermal dysplasia-clefting syndrome who developed non-Hodgkin lymphoma.

Balci S, Engiz O, Okten G, Sipahier M, Gursu G, Kandemir B
Oral surgery, oral medicine, oral pathology, oral radiology, and endodontics 2009 Sep;108(3):e91-5

Mesenchymal stem cells transduced by PLEGFP-N1 retroviral vector maintain their biological features and differentiation.

He X, Li YL, Wang XR, Guo X, Niu Y
Chinese medical journal 2005 Oct 20;118(20):1728-34

CIK cells from patients with HCC possess strong cytotoxicity to multidrug-resistant cell line Bel-7402/R.

Zhang YS, Yuan FJ, Jia GF, Zhang JF, Hu LY, Huang L, Wang J, Dai ZQ
World journal of gastroenterology 2005 Jun 14;11(22):3339-45

CIK cells from patients with HCC possess strong cytotoxicity to multidrug-resistant cell line Bel-7402/R.

Zhang YS, Yuan FJ, Jia GF, Zhang JF, Hu LY, Huang L, Wang J, Dai ZQ
World journal of gastroenterology 2005 Jun 14;11(22):3339-45

Invasive inflammatory pseudotumor of uterine cervix: a case report.

Gücer F, Altaner S, Mülayim N, Yapicier O
Gynecologic oncology 2005 Aug;98(2):325-8

Involvement of the Fas/FasL pathway in the pathogenesis of germ cell tumours of the adult testis.

Kersemaekers AM, van Weeren PC, Oosterhuis JW, Looijenga LH
The Journal of pathology 2002 Apr;196(4):423-9

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of CD45 was performed by loading 25 µg of Daudi (lane 1) and CEM (lane 2) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a CD45 monoclonal antibody (Product # MA5-13197) at a dilution of 1:50 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at ~200 kDa.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Formalin-fixed, paraffin-embedded human tonsil stained with CD45 antibody using peroxidase-conjugate and AEC chromogen. Note cell membrane staining of leucocytes.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of CD45 in Jurkat cells (green) compared to an isotype control (blue). Cells were harvested, adjusted to a concentration of 1-5x10^6 cells/mL, fixed with 2% paraformaldehyde and washed with PBS. Cells were blocked with a 2% solution of BSA-PBS for 30 min at room temperature and incubated with a CD45 monoclonal antibody (Product # MA5-13197) at a dilution of 0.5 µg/test for 60 min at room temperature. Cells were then incubated for 40 min at room temperature in the dark using a Dylight 488-conjugated goat anti-mouse IgG (H+L) secondary antibody and re-suspended in PBS for FACS analysis.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of CD45 in PBMC cells (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 µL of cell solution was added to each tube at a dilution of 2x10^7 cells/mL, followed by the addition of 50 µL of isotype control and primary antibody (Product # MA5-13197) at a dilution of 0.5 µg/test. Cells were incubated for 30 min at 4ºC and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated goat anti-mouse IgG (H+L) secondary for 30 min at 4ºC in the dark. FACS analysis was performed using 400 µL of cell buffer.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry analysis of CD45 in PBMC cells (green) compared to an isotype control (blue). Human blood was collected, combined with a hydrophilic polysaccharide, centrifuged, transferred to a conical tube and washed with PBS. 50 µL of cell solution was added to each tube at a dilution of 2x10^7 cells/mL, followed by the addition of 50 µL of isotype control and primary antibody (Product # MA5-13197) at a dilution of 0.5 µg/test. Cells were incubated for 30 min at 4ºC and washed with a cell buffer, followed by incubation with a DyLight 488-conjugated goat anti-mouse IgG (H+L) secondary for 30 min at 4ºC in the dark. FACS analysis was performed using 400 µL of cell buffer.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 Exosome characterization. ( A ) Representative transmission electron microscopy pictures of CD45(-) (left) and total (middle and right) exosomes. ( B ) Western blot for detection of TSG 101, EpCAM and CD45 of total, CD45(-) and (+) exosomes from plasma of head and neck squamous cell carcinoma (HNSCC) patients (#1&2 high stage). Note that no CD45 signal was detected when using CD45(-) exosomes. Uncropped Western blots can be found at Figure S1 . ( C ) Nanoparticle tracking analysis with ZetaView of total (left) and CD45(-) exosomes (right). Vesicles between 30 and 200 nm diameter were detected.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 Verification of OOM-SCs Characteristics. ( A ) Microscopic images depict the CFU of OOM-SCs that were visualized with 0.15% crystal violet solution after two weeks of culture. Images of the stained colonies were captured using phase-contrast microscopy. ( B ) RT-PCR results of the expression of the stemness markers Nanog, Sox2, and Rex1 in comparison with those of WJ-MSCs. GAPDH was used as a housekeeping gene. ( C ) RT-PCR results of specific markers, including ITGA6, CD146, and TM4SF1, distinguish between multipotent MSCs and NHDFs. These genes are only expressed in multipotent MSCs. ( D ) RT-PCR data for the expression levels of the surface markers of the isolated OOM-SCs compared with those of WJ-MSCs. These markers included the negatively (CD19 and CD45) and positively (CD73, CD105, and CD166) expressed markers. ( E ) FACS analysis for confirming the expression of the negative markers CD34 and CD45 and positive markers CD73, CD90, and CD105 for OOM-SCs, compared with those of WJ-MSCs. FACS results are summarized in the table (right panel), and all results are presented in percentages. ( F ) Microscopic images of adipogenic, chondrogenic, and osteogenic differentiation were evaluated by Oil Red, Alcian Blue, and Alizarin Red S staining, respectively. The upper and lower panels represent undifferentiated and differentiated cells, respectively. Scale bar = 200 mum.

MA5-13197

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