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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB1430 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CD45 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human CD45. This antibody recognizes all isoforms of human CD45.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 2D1
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references FGL2 promotes tumor progression in the CNS by suppressing CD103(+) dendritic cell differentiation.
Optimal design, anti-tumour efficacy and tolerability of anti-CXCR4 antibody drug conjugates.
Fluoxetine induces direct inhibitory effects on mesenchymal stem cell‑derived osteoprogenitor cells independent of serotonin concentration.
Potential role of CBX7 in regulating pluripotency of adult human pluripotent-like olfactory stem cells in stroke model.
Preferential Lineage-Specific Differentiation of Osteoblast-Derived Induced Pluripotent Stem Cells into Osteoprogenitors.
Proteomic profiling of peripheral blood neutrophils identifies two inflammatory phenotypes in stable COPD patients.
Regional Differences Between Perisynovial and Infrapatellar Adipose Tissue Depots and Their Response to Class II and Class III Obesity in Patients With Osteoarthritis.
Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against Stroke.
Switch from canonical to noncanonical Wnt signaling mediates high glucose-induced adipogenesis.
Isolation and characterization of synovial mesenchymal stem cells.
Yan J, Zhao Q, Gabrusiewicz K, Kong LY, Xia X, Wang J, Ott M, Xu J, Davis RE, Huo L, Rao G, Sun SC, Watowich SS, Heimberger AB, Li S
Nature communications 2019 Jan 25;10(1):448
Nature communications 2019 Jan 25;10(1):448
Optimal design, anti-tumour efficacy and tolerability of anti-CXCR4 antibody drug conjugates.
Costa MJ, Kudaravalli J, Ma JT, Ho WH, Delaria K, Holz C, Stauffer A, Chunyk AG, Zong Q, Blasi E, Buetow B, Tran TT, Lindquist K, Dorywalska M, Rajpal A, Shelton DL, Strop P, Liu SH
Scientific reports 2019 Feb 21;9(1):2443
Scientific reports 2019 Feb 21;9(1):2443
Fluoxetine induces direct inhibitory effects on mesenchymal stem cell‑derived osteoprogenitor cells independent of serotonin concentration.
Koura SM, Salama M, El-Hussiny M, Khalil MEA, Lotfy A, Hassan SA, Gad Elhak SA, Sobh MA
Molecular medicine reports 2019 Apr;19(4):2611-2619
Molecular medicine reports 2019 Apr;19(4):2611-2619
Potential role of CBX7 in regulating pluripotency of adult human pluripotent-like olfactory stem cells in stroke model.
Fan JR, Lee HT, Lee W, Lin CH, Hsu CY, Hsieh CH, Shyu WC
Cell death & disease 2018 May 1;9(5):502
Cell death & disease 2018 May 1;9(5):502
Preferential Lineage-Specific Differentiation of Osteoblast-Derived Induced Pluripotent Stem Cells into Osteoprogenitors.
Roberts CL, Chen SS, Murchison AC, Ogle RA, Francis MP, Ogle RC, Sachs PC
Stem cells international 2017;2017:1513281
Stem cells international 2017;2017:1513281
Proteomic profiling of peripheral blood neutrophils identifies two inflammatory phenotypes in stable COPD patients.
Loi ALT, Hoonhorst S, van Aalst C, Langereis J, Kamp V, Sluis-Eising S, Ten Hacken N, Lammers JW, Koenderman L
Respiratory research 2017 May 22;18(1):100
Respiratory research 2017 May 22;18(1):100
Regional Differences Between Perisynovial and Infrapatellar Adipose Tissue Depots and Their Response to Class II and Class III Obesity in Patients With Osteoarthritis.
Harasymowicz NS, Clement ND, Azfer A, Burnett R, Salter DM, Simpson AHWR
Arthritis & rheumatology (Hoboken, N.J.) 2017 Jul;69(7):1396-1406
Arthritis & rheumatology (Hoboken, N.J.) 2017 Jul;69(7):1396-1406
Feasibility and Efficiency of Human Bone Marrow Stromal Cell Culture with Allogeneic Platelet Lysate-Supplementation for Cell Therapy against Stroke.
Tan C, Shichinohe H, Wang Z, Hamauchi S, Abumiya T, Nakayama N, Kazumata K, Ito T, Kudo K, Takamoto S, Houkin K
Stem cells international 2016;2016:6104780
Stem cells international 2016;2016:6104780
Switch from canonical to noncanonical Wnt signaling mediates high glucose-induced adipogenesis.
Keats EC, Dominguez JM 2nd, Grant MB, Khan ZA
Stem cells (Dayton, Ohio) 2014 Jun;32(6):1649-60
Stem cells (Dayton, Ohio) 2014 Jun;32(6):1649-60
Isolation and characterization of synovial mesenchymal stem cells.
Harvanová D, Tóthová T, Sarišský M, Amrichová J, Rosocha J
Folia biologica 2011;57(3):119-24
Folia biologica 2011;57(3):119-24
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CD45 in Human PBMCs. CD45 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA blast using Mouse Anti-Human CD45 Monoclonal Antibody (Catalog # MAB1430) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CD45 in Hodgkin's Lymphoma Tissue. CD45 was detected in immersion fixed paraffin-embedded sections of Hodgkins lymphoma tissue using Mouse Anti-Human CD45 Monoclonal Antibody (Catalog # MAB1430) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell surfaces. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CD45 in Human Blood Lymphocytes by Flow Cytometry. Human peripheral blood lymphocytes were stained with Mouse Anti-Human CD45 Monoclonal Antibody (Catalog # MAB1430, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.
