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LearnMA1-12600
antibody from Invitrogen Antibodies
Targeting: ESR1
ER-alpha, Era, ESR, NR3A1
Western blot
ELISA Immunocytochemistry Immunoprecipitation Immunohistochemistry Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [17]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- MA1-12600 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Estrogen Receptor alpha Monoclonal Antibody (AER311)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Bovine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- AER311
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Endometrial expression of progesterone, estrogen, and oxytocin receptors and of 20α-hydroxysteroid dehydrogenase and cyclooxygenase II 2 and 5 days after ovulation in induced short and normal estrous cycles in dairy cows.
Guggulsterone-mediated enhancement of radiosensitivity in human tumor cell lines.
Analytical Validation of a Highly Quantitative, Sensitive, Accurate, and Reproducible Assay (HERmark) for the Measurement of HER2 Total Protein and HER2 Homodimers in FFPE Breast Cancer Tumor Specimens.
A comparison of oestrogen receptor and progesterone receptor expression in endometrial polyps and endometrium of premenopausal women.
Immunohistochemical localization of oestrogen receptors alpha and beta, progesterone receptor and aromatase in the equine placenta.
Estrogen-dependent signaling in a molecularly distinct subclass of aggressive prostate cancer.
The tumor suppressor activity of the lysyl oxidase propeptide reverses the invasive phenotype of Her-2/neu-driven breast cancer.
HER-2/neu x aromatase double transgenic mice model: the effects of aromatase overexpression on mammary tumorigenesis.
Tuberin nuclear localization can be regulated by phosphorylation of its carboxyl terminus.
Estrogen receptor alpha and beta immunoreactive neurons in normal adult and aged female rat hippocampus: a qualitative and quantitative study.
Hyaluronan-CD44 interaction with IQGAP1 promotes Cdc42 and ERK signaling, leading to actin binding, Elk-1/estrogen receptor transcriptional activation, and ovarian cancer progression.
Conjugated linoleic acid blocks estrogen signaling in human breast cancer cells.
Occurrence of estrogen receptor alpha in bovine placentomes throughout mid and late gestation and at parturition.
Occurrence of estrogen receptor alpha in bovine placentomes throughout mid and late gestation and at parturition.
Affinity chromatography of human estrogen receptor-alpha expressed in Saccharomyces cerevisiae. Combination of heparin- and 17beta-estradiol-affinity chromatography.
Affinity chromatography of human estrogen receptor-alpha expressed in Saccharomyces cerevisiae. Combination of heparin- and 17beta-estradiol-affinity chromatography.
Expression of estrogen receptor variant messenger RNAs and determination of estrogen receptor status in human breast cancer.
Rantala MH, Mutikainen M, Schuler G, Katila T, Taponen J
Theriogenology 2014 Jun;81(9):1181-8
Theriogenology 2014 Jun;81(9):1181-8
Guggulsterone-mediated enhancement of radiosensitivity in human tumor cell lines.
Choudhuri R, Degraff W, Gamson J, Mitchell JB, Cook JA
Frontiers in oncology 2011;1:19
Frontiers in oncology 2011;1:19
Analytical Validation of a Highly Quantitative, Sensitive, Accurate, and Reproducible Assay (HERmark) for the Measurement of HER2 Total Protein and HER2 Homodimers in FFPE Breast Cancer Tumor Specimens.
Larson JS, Goodman LJ, Tan Y, Defazio-Eli L, Paquet AC, Cook JW, Rivera A, Frankson K, Bose J, Chen L, Cheung J, Shi Y, Irwin S, Kiss LD, Huang W, Utter S, Sherwood T, Bates M, Weidler J, Parry G, Winslow J, Petropoulos CJ, Whitcomb JM
Pathology research international 2010 Jun 28;2010:814176
Pathology research international 2010 Jun 28;2010:814176
A comparison of oestrogen receptor and progesterone receptor expression in endometrial polyps and endometrium of premenopausal women.
Peng X, Li T, Xia E, Xia C, Liu Y, Yu D
Journal of obstetrics and gynaecology : the journal of the Institute of Obstetrics and Gynaecology 2009 May;29(4):340-6
Journal of obstetrics and gynaecology : the journal of the Institute of Obstetrics and Gynaecology 2009 May;29(4):340-6
Immunohistochemical localization of oestrogen receptors alpha and beta, progesterone receptor and aromatase in the equine placenta.
Abd-Elnaeim MM, Derar IR, Wilsher S, Allen WR, Leiser R, Schuler G
Reproduction in domestic animals = Zuchthygiene 2009 Apr;44(2):312-9
Reproduction in domestic animals = Zuchthygiene 2009 Apr;44(2):312-9
Estrogen-dependent signaling in a molecularly distinct subclass of aggressive prostate cancer.
Setlur SR, Mertz KD, Hoshida Y, Demichelis F, Lupien M, Perner S, Sboner A, Pawitan Y, Andrén O, Johnson LA, Tang J, Adami HO, Calza S, Chinnaiyan AM, Rhodes D, Tomlins S, Fall K, Mucci LA, Kantoff PW, Stampfer MJ, Andersson SO, Varenhorst E, Johansson JE, Brown M, Golub TR, Rubin MA
Journal of the National Cancer Institute 2008 Jun 4;100(11):815-25
Journal of the National Cancer Institute 2008 Jun 4;100(11):815-25
The tumor suppressor activity of the lysyl oxidase propeptide reverses the invasive phenotype of Her-2/neu-driven breast cancer.
Min C, Kirsch KH, Zhao Y, Jeay S, Palamakumbura AH, Trackman PC, Sonenshein GE
Cancer research 2007 Feb 1;67(3):1105-12
Cancer research 2007 Feb 1;67(3):1105-12
HER-2/neu x aromatase double transgenic mice model: the effects of aromatase overexpression on mammary tumorigenesis.
Tekmal RR, Nair HB, Perla RP, Kirma N
The Journal of steroid biochemistry and molecular biology 2007 Aug-Sep;106(1-5):111-8
The Journal of steroid biochemistry and molecular biology 2007 Aug-Sep;106(1-5):111-8
Tuberin nuclear localization can be regulated by phosphorylation of its carboxyl terminus.
York B, Lou D, Noonan DJ
Molecular cancer research : MCR 2006 Nov;4(11):885-97
Molecular cancer research : MCR 2006 Nov;4(11):885-97
Estrogen receptor alpha and beta immunoreactive neurons in normal adult and aged female rat hippocampus: a qualitative and quantitative study.
Mehra RD, Sharma K, Nyakas C, Vij U
Brain research 2005 Sep 14;1056(1):22-35
Brain research 2005 Sep 14;1056(1):22-35
Hyaluronan-CD44 interaction with IQGAP1 promotes Cdc42 and ERK signaling, leading to actin binding, Elk-1/estrogen receptor transcriptional activation, and ovarian cancer progression.
Bourguignon LY, Gilad E, Rothman K, Peyrollier K
The Journal of biological chemistry 2005 Mar 25;280(12):11961-72
The Journal of biological chemistry 2005 Mar 25;280(12):11961-72
Conjugated linoleic acid blocks estrogen signaling in human breast cancer cells.
Tanmahasamut P, Liu J, Hendry LB, Sidell N
The Journal of nutrition 2004 Mar;134(3):674-80
The Journal of nutrition 2004 Mar;134(3):674-80
Occurrence of estrogen receptor alpha in bovine placentomes throughout mid and late gestation and at parturition.
Schuler G, Wirth C, Teichmann U, Failing K, Leiser R, Thole H, Hoffmann B
Biology of reproduction 2002 Apr;66(4):976-82
Biology of reproduction 2002 Apr;66(4):976-82
Occurrence of estrogen receptor alpha in bovine placentomes throughout mid and late gestation and at parturition.
Schuler G, Wirth C, Teichmann U, Failing K, Leiser R, Thole H, Hoffmann B
Biology of reproduction 2002 Apr;66(4):976-82
Biology of reproduction 2002 Apr;66(4):976-82
Affinity chromatography of human estrogen receptor-alpha expressed in Saccharomyces cerevisiae. Combination of heparin- and 17beta-estradiol-affinity chromatography.
Feng W, Graumann K, Hahn R, Jungbauer A
Journal of chromatography. A 1999 Aug 6;852(1):161-73
Journal of chromatography. A 1999 Aug 6;852(1):161-73
Affinity chromatography of human estrogen receptor-alpha expressed in Saccharomyces cerevisiae. Combination of heparin- and 17beta-estradiol-affinity chromatography.
Feng W, Graumann K, Hahn R, Jungbauer A
Journal of chromatography. A 1999 Aug 6;852(1):161-73
Journal of chromatography. A 1999 Aug 6;852(1):161-73
Expression of estrogen receptor variant messenger RNAs and determination of estrogen receptor status in human breast cancer.
Huang A, Leygue ER, Snell L, Murphy LC, Watson PH
The American journal of pathology 1997 May;150(5):1827-33
The American journal of pathology 1997 May;150(5):1827-33
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Estrogen Receptor alpha Monoclonal Antibody (AER311) (Product # PA1-12600) and a 66kDa band corresponding to Estrogen Receptor alpha was observed across all the cell lines tested. Whole cell extracts (30 µg lysate) of T-47D (Lane 1), MCF-7 (Lane 2), SK-BR-3 (Lane 3), HepG2 (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Estrogen Receptor alpha was performed using 70% confluent log phase T-47D cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with beta Estrogen Receptor alpha Monoclonal Antibody (AER311) (Product # MA1-12600) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32723), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing nuclear localization. Panel e represents merged image for SK-BR-3 cells showing no staining for Estrogen Receptor alpha. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation (ChIP) assay of endogenous Estrogen Receptor alpha protein using ESR alpha Antibody: ChIP was performed using Anti-Estrogen Receptor alpha Monoclonal Antibody (AER311) (Product # MA1-12600, 2.5 µg) on sheared chromatin from MCF7 cells treated with beta estradiol (10 nm for 45 mins) using the MAGnify ChIP System kit (Product # 49-2024). Chromatin from untreated MCF7 cells were prepared the same way. Normal Mouse IgG was used as a negative IP control. The purified DNA was analyzed by qPCR using primers binding to XBP1, GREB1 (Active) and GAPDH (+4.5kb downstream of promoter) and SAT2 satellite repeats (Inactive). Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.
