Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
- Flow cytometry [2]
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Validation data
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- Product number
- NBP2-33321-0.1mg - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal ER alpha/NR3A1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. This MAb is specific to ER alpha and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. It strongly stains nuclei of epithelial cells in breast carcinomas.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: ER alpha/NR3A1 Antibody (ER506) - Azide and BSA Free [NBP2-33321] - Electropherogram image of the corresponding Simple Western lane. Estrogen Receptor antibody was used at 10 ug/ml dilution of MCF-7 and T47D lysates(s) respectively.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: ER alpha/NR3A1 Antibody (ER506) - Azide and BSA Free [NBP2-33321] - Simple Western lane view shows a specific band for Estrogen Receptor in 0.2 mg/ml of MCF-7 (left) and T47D (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ER alpha/NR3A1 Antibody (ER506) - Azide and BSA Free [NBP2-33321] - Western Blot Analysis of human MCF-7 cell lysate using ER alpha/NR3A1 Antibody (ER506).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (ER506) - Azide and BSA Free [NBP2-33321] - Human breast invasive ductal carcinoma stained with PE conjugated Estrogen Receptor MAb (ER506).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (ER506) - Azide and BSA Free [NBP2-33321] - Formalin-fixed, paraffin-embedded human Endometrium stained with ER alpha/NR3A1 Antibody (ER506).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: ER alpha/NR3A1 Antibody (ER506) - Azide and BSA Free [NBP2-33321] - Flow Cytometry for human ER-alpha on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled ER alpha/NR3A1 Antibody (ER506).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow (Intracellular): ER alpha/NR3A1 Antibody (ER506) - Azide and BSA Free [NBP2-33321] - Staining of MCF-7 cells using PE conjugated Estrogen Receptor MAb (ER506). Paraformaldehyde with 0.1% Tween-PBS was used to permealize the cells.