Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Chromatin Immunoprecipitation [2]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 49-1002 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Estrogen Receptor alpha Monoclonal Antibody
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 50 µg
- Concentration
- 2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Neonatal overexpression of estrogen receptor-α alters midbrain dopamine neuron development and reverses the effects of low maternal care in female offspring.
Correlation of immunoglobulin G expression and histological subtype and stage in breast cancer.
Peña CJ, Champagne FA
Developmental neurobiology 2015 Oct;75(10):1114-24
Developmental neurobiology 2015 Oct;75(10):1114-24
Correlation of immunoglobulin G expression and histological subtype and stage in breast cancer.
Yang B, Ma C, Chen Z, Yi W, McNutt MA, Wang Y, Korteweg C, Gu J
PloS one 2013;8(3):e58706
PloS one 2013;8(3):e58706
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Recombinant ERα (right side) and recombinant ERβ1 (left side) (100 fmol each per well) were analyzed by western blot using the anti-hERα monoclonal antibody (clone: mAN1; Product # 49-1002) at 7 µg⁄ml. The antibody is clearly recognizing the ERα isoform and not ERβ. (Note that the antibody is not recommended for use in WB for detection of endogenous low levels of ER expression.)
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Recombinant ERα (right side) and recombinant ERβ1 (left side) (100 fmol each per well) were analyzed by western blot using the anti-hERα monoclonal antibody (clone: mAN1; Product # 49-1002) at 7 µg⁄ml. The antibody is clearly recognizing the ERα isoform and not ERβ. (Note that the antibody is not recommended for use in WB for detection of endogenous low levels of ER expression.)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- COS-7 cells transiently overexpressing human ERα (left panel) or ERβ1 (right panel) were both labeled with the anti-ERα antibody followed by biotinylated secondary antibody and peroxidase-labeled avidin. The monoclonal antibody directed against ERα (clone: mAN1, Product # 49-1002) is used at 15 µg⁄ml. The antibody is clearly recognizing the ERα isoform and not ERβ
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ChIP assays were performed using MCF7 cells, the anti-ERα monoclonal antibody (Product # 49-1002; clone: mAN1) and optimized PCR primer sets for qPCR, . The cells were treated with estradiol (ER agonist) for 3 hours prior to cell harvesting. Each ChIP assay used sheared chromatin from 3 million cells and 5 µg of anti-hERα antibody. Recovery (%: ChIP⁄input) and occupancy (x fold: positive⁄negative) are shown here. Recovery (red bar) and occupancy (yellow bar) of human GREB1 promoter by ERα, respectively. (Recovery of human myoglobin exon 2 (myo ex2) by ERα is shown as a negative control.) Occupancy of the human GREB1 promoter by ERα is evident based on fluorescent qPCR analysis of immunoprecipitated DNA. Controls for IP and PCR specificity include antibody directed against ERβ (data not shown) and primers for human myoglobin exon 2 (myo ex2; negative control) respectively.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ChIP assays were performed using MCF7 cells, the anti-ERα monoclonal antibody (Product # 49-1002; clone: mAN1) and optimized PCR primer sets for qPCR, . The cells were treated with estradiol (ER agonist) for 3 hours prior to cell harvesting. Each ChIP assay used sheared chromatin from 3 million cells and 5 µg of anti-hERα antibody. Recovery (%: ChIP⁄input) and occupancy (x fold: positive⁄negative) are shown here. Recovery (red bar) and occupancy (yellow bar) of human GREB1 promoter by ERα, respectively. (Recovery of human myoglobin exon 2 (myo ex2) by ERα is shown as a negative control.) Occupancy of the human GREB1 promoter by ERα is evident based on fluorescent qPCR analysis of immunoprecipitated DNA. Controls for IP and PCR specificity include antibody directed against ERβ (data not shown) and primers for human myoglobin exon 2 (myo ex2; negative control) respectively.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
