Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-37570 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-Estrogen Receptor alpha (Ser167) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control for Western blot is MCF-7 cells; suggested positive control for IHC is human breast carcinoma; suggested positive control for ICC/IF is MCF-7 cells.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Vps11 and Vps18 of Vps-C membrane traffic complexes are E3 ubiquitin ligases and fine-tune signalling.
Icariin, but Not Genistein, Exerts Osteogenic and Anti-apoptotic Effects in Osteoblastic Cells by Selective Activation of Non-genomic ERα Signaling.
FGFR2-Driven Signaling Counteracts Tamoxifen Effect on ERα-Positive Breast Cancer Cells.
Segala G, Bennesch MA, Ghahhari NM, Pandey DP, Echeverria PC, Karch F, Maeda RK, Picard D
Nature communications 2019 Apr 23;10(1):1833
Nature communications 2019 Apr 23;10(1):1833
Icariin, but Not Genistein, Exerts Osteogenic and Anti-apoptotic Effects in Osteoblastic Cells by Selective Activation of Non-genomic ERα Signaling.
Ho MX, Poon CC, Wong KC, Qiu ZC, Wong MS
Frontiers in pharmacology 2018;9:474
Frontiers in pharmacology 2018;9:474
FGFR2-Driven Signaling Counteracts Tamoxifen Effect on ERα-Positive Breast Cancer Cells.
Turczyk L, Kitowska K, Mieszkowska M, Mieczkowski K, Czaplinska D, Piasecka D, Kordek R, Skladanowski AC, Potemski P, Romanska HM, Sadej R
Neoplasia (New York, N.Y.) 2017 Oct;19(10):791-804
Neoplasia (New York, N.Y.) 2017 Oct;19(10):791-804
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from MCF-7 cells using Estrogen Receptor-alpha (pSer167) polyclonal antibody (Product # PA5-37570) (Lane 2) and the same antibody preincubated with blocking peptide (Lane 1).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of Phospho-Estrogen Receptor alpha (Ser167) in methanol-fixed MCF cells, using Phospho-Estrogen Receptor alpha (Ser167) Polyclonal Antibody (Product # PA5-37570).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-Estrogen Receptor alpha (Ser167) in paraffin-embedded Human breast carcinoma tissue using Phospho-Estrogen Receptor alpha (Ser167) Polyclonal Antibody (Product # PA5-37570) (left) or the same antibody preincubated with blocking peptide (right).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- FIGURE 3 The acute effects of genistein and icariin on phosphorylation of ERalpha (Ser118) and ERalpha (Ser167) in MC3T3-E1 cells and UMR-106 cells. Cells were treated with vehicle (C), 17beta-estradiol (E2; 10 nM), genistein (GEN; 10 nM), or icariin (ICA; 0.1 muM) for 10 min. Representative immunoblots showing the protein expressions of p-ERalpha (Ser118), p-ERalpha (Ser167), ERalpha, and beta-actin (A) in MC3T3-E1 cells and (D) transfected UMR-106 cells. The ratio of p-ERalpha (Ser118)/ERalpha protein expression in (B) MC3T3-E1 cells and (E) transfected UMR-106 cells and the ratio of p-ERalpha (Ser167)/ERalpha protein expression in (C) MC3T3-E1 cells and (F) transfected UMR-106 cells were shown. Results were expressed as mean +- SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001 versus the control ( n = 3).