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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-37571 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-Estrogen Receptor alpha (Tyr537) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control for Western blot is K562 cells; suggested positive control for IHC is human lung carcinoma.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20°C
Submitted references Vps11 and Vps18 of Vps-C membrane traffic complexes are E3 ubiquitin ligases and fine-tune signalling.
Segala G, Bennesch MA, Ghahhari NM, Pandey DP, Echeverria PC, Karch F, Maeda RK, Picard D
Nature communications 2019 Apr 23;10(1):1833
Nature communications 2019 Apr 23;10(1):1833
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of extracts from K562 cells (Lane 2) using Estrogen Receptor-alpha (pTyr537) polyclonal antibody (Product # PA5-37571) compared to the same antibody preincubated with a blocking peptide (Lane 1).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-Estrogen Receptor alpha (Tyr537) in paraffin-embedded Human lung carcinoma tissue using Phospho-Estrogen Receptor alpha (Tyr537) Polyclonal Antibody (Product # PA5-37571) (left) or the same antibody preincubated with blocking peptide (right).
