Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NB300-560 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-560, RRID:AB_10001032
- Product name
- Mouse Monoclonal ER alpha/NR3A1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Detects both the steroid occupied and unoccupied forms of the estrogen receptor (ER) alpha. This does not cross react with ER beta, androgen receptor, progesterone receptor, or glucocorticoid receptor.
- Reactivity
- Human, Mouse, Rat, Bovine, Chicken/Avian, Simian
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100ug
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Impact of perinatal bisphenol A and 17β estradiol exposure: Comparing hormone receptor response.
Impact of caudal hindbrain glycogen metabolism on A2 noradrenergic neuron AMPK activation and ventromedial hypothalamic nucleus norepinephrine activity and glucoregulatory neurotransmitter marker protein expression.
Sex-dimorphic estrogen receptor regulation of ventromedial hypothalamic nucleus glucoregulatory neuron adrenergic receptor expression in hypoglycemic male and female rats.
Role of Brf1 interaction with ERα, and significance of its overexpression, in human breast cancer.
Development and characterization of monoclonal antibodies to a specific domain of human estrogen receptor.
Development and characterization of monoclonal antibodies to a specific domain of human estrogen receptor.
Leonel ECR, Campos SGP, Guerra LHA, Bedolo CM, Vilamaior PSL, Calmon MF, Rahal P, Amorim CA, Taboga SR
Ecotoxicology and environmental safety 2020 Jan 30;188:109918
Ecotoxicology and environmental safety 2020 Jan 30;188:109918
Impact of caudal hindbrain glycogen metabolism on A2 noradrenergic neuron AMPK activation and ventromedial hypothalamic nucleus norepinephrine activity and glucoregulatory neurotransmitter marker protein expression.
Alshamrani AA, Bheemanapally K, Ibrahim MMH, Briski KP
Neuropeptides 2020 Aug;82:102055
Neuropeptides 2020 Aug;82:102055
Sex-dimorphic estrogen receptor regulation of ventromedial hypothalamic nucleus glucoregulatory neuron adrenergic receptor expression in hypoglycemic male and female rats.
Uddin MM, Mahmood ASMH, Ibrahim MMH, Briski KP
Brain research 2019 Oct 1;1720:146311
Brain research 2019 Oct 1;1720:146311
Role of Brf1 interaction with ERα, and significance of its overexpression, in human breast cancer.
Fang Z, Yi Y, Shi G, Li S, Chen S, Lin Y, Li Z, He Z, Li W, Zhong S
Molecular oncology 2017 Dec;11(12):1752-1767
Molecular oncology 2017 Dec;11(12):1752-1767
Development and characterization of monoclonal antibodies to a specific domain of human estrogen receptor.
Traish AM, Ettinger R, Kim N, Marshak-Rothstein A, Wotiz HH
Steroids 1990 May;55(5):196-208
Steroids 1990 May;55(5):196-208
Development and characterization of monoclonal antibodies to a specific domain of human estrogen receptor.
Traish AM, Ettinger R, Kim N, Marshak-Rothstein A, Wotiz HH
Steroids 1990 May;55(5):196-208
Steroids 1990 May;55(5):196-208
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ER alpha/NR3A1 Antibody (33) [NB300-560] - Analysis of recombinant ER alpha.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast tissue (B) and magnified section (C) compared with an isotype control (A).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: ER alpha/NR3A1 Antibody (33) [NB300-560] - Human breast carcinoma (B) and magnified section (C) compared with an isotype control (A).