V2113

antibody from NSJ Bioreagents

Targeting: ESR1 ER-alpha, Era, ESR, NR3A1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Protein array (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

V2113

Provider

NSJ Bioreagents

Product name

ER Antibody / Estrogen Receptor alpha

Antibody type

Monoclonal

Description

This highly specific ER antibody is suitable for use in Flow cytometry/Western blot/Immunohistochemistry/Immunofluorescence applications with human samples.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

ER506

Vial size

20 ug (with BSA and sodium azide), 100 ug (with BSA and sodium azide), 100 ug (without BSA or sodium azide), 7 ml IHC only format (if applicable)

Concentration

0.2 mg/ml, 1 mg/ml

Storage

Store the ER antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of MCF-7 cell lysate with ER antibody (clone ER506). Predicted molecular weight of ER alpha: ~66kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of MCF-7 cell lysate with ER antibody (clone ER506). Predicted molecular weight of ER alpha: ~67 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Immunofluorescent staining of methanol-fixed human MCF7 cells with ER antibody (clone ER506, green) and Reddot nuclear stain (red).

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of human breast invasive ductal carcinoma with ER antibody (ER506).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human endometrium with ER antibody (clone ER506).

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

FACS: intracellular flow staining of MCF-7 cells using ER antibody (red) and isotype control (green).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

FACS testing of human MCF-7: Black=cells alone; Green=isotype control; Red=PE conjugate (V2113PE)

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Intracellular FACS testing of human MCF-7 cells: Black=cells alone; Green=isotype control; Red= PE conjugated ER antibody (V2113PE)

Protein array

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Analysis of HuProt(TM) microarray containing more than 19,000 full-length human proteins using ER antibody (clone ER506). These results demonstrate the foremost specificity of the ER506 mAb.Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

Other assay

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

SDS-PAGE analysis of purified, BSA-free ER antibody (clone ER506) as confirmation of integrity and purity.