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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- MAB0118 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB0118, RRID:AB_1019101
- Product name
- ESR1 monoclonal antibody, clone mAN1
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against synthetic peptide of ESR1.
- Isotype
- IgG
- Antibody clone number
- mAN1
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Analysis by Western blot of ESR1 using the ESR1 monoclonal antibody, clone mAN1 (Cat # MAB0118). Specificity of the antibody is assessed by Western blot using recombinant ESR1 (right side) and recombinant ESR2 (left side), 100 fmol each per well. The monoclonal antibody directed against ESR1 monoclonal antibody, clone mAN1 (Cat # MAB0118) is used at 7 ug/mL . The antibody is clearly recognizing the ESR1 isoform and not ESR2. (Note that the antibody is not recommended for use in WB for detection of endogenous low levels of ESR expression.)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunocytochemistry using the ESR1 monoclonal antibody, clone mAN1 (Cat # MAB0118). COS-7 cells transiently overexpressing human ESR1 (left panel) or ESR2 (right panel) were both labeled with the ESR1 monoclonal antibody, clone mAN1 (Cat # MAB0118) followed by biotinylated second antibody and peroxidase-labeled avidin. The monoclonal antibody directed against ESR1 is used at 15 ug/mL . The antibody is clearly recognizing the ESR1 isoform and not ESR2.
- Validation comment
- Immunocytochemistry
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ChIP results obtained with the ESR1 monoclonal antibody, clone mAN1 (Cat # MAB0118). ChIP assays were performed using MCF-7 cells.
- Validation comment
- ChIP
