Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB8304 - Provider product page

- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB8304, RRID:AB_10629940
- Product name
- ESR1 monoclonal antibody, clone 5D4B1,5D4E3
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against partial recombinant ESR1.
- Isotype
- IgG
- Antibody clone number
- 5D4B1,5D4E3
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Phosphatidylinositol 3-kinase/AKT-mediated activation of estrogen receptor alpha: a new model for anti-estrogen resistance.
Activation of estrogen receptor alpha by S118 phosphorylation involves a ligand-dependent interaction with TFIIH and participation of CDK7.
Campbell RA, Bhat-Nakshatri P, Patel NM, Constantinidou D, Ali S, Nakshatri H
The Journal of biological chemistry 2001 Mar 30;276(13):9817-24
The Journal of biological chemistry 2001 Mar 30;276(13):9817-24
Activation of estrogen receptor alpha by S118 phosphorylation involves a ligand-dependent interaction with TFIIH and participation of CDK7.
Chen D, Riedl T, Washbrook E, Pace PE, Coombes RC, Egly JM, Ali S
Molecular cell 2000 Jul;6(1):127-37
Molecular cell 2000 Jul;6(1):127-37
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image

- Experimental details
- Western blot analysis using ESR1 monoclonal antibody, clone 5D4B1, 5D4E3 (Cat # MAB8304) against truncated ESR1 recombinant protein (Lane 1) and MCF-7 cell lysate (Lane 2).