Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- M00057-2 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-ER alpha ESR1 Rabbit Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Monoclonal antibody for ESTROGEN RECEPTOR ALPHA/ESR1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: ChIP, Flow Cytometry, IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat, Dog. ESTROGEN RECEPTOR ALPHA/ESR1 information: Molecular Weight: 66216 MW; Subcellular Localization: Isoform 1: Nucleus . Cytoplasm . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . A minor fraction is associated with the inner membrane; Tissue Specificity: Widely expressed. Isoform 3 is not expressed in the pituitary gland.
- Reactivity
- Human, Mouse, Rat, Canine
- Host
- Rabbit
- Antibody clone number
- IE-5
- Vial size
- 100ug/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. Avoid repeated freezing and thawing.
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human cervix carcinoma, using ER alpha Antibody(M00057-2)ESR1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ESR1 Antibody (M00057-2)overnight at 4
- Additional image