NBP1-41192

antibody from Novus Biologicals

Targeting: ESR1 ER-alpha, Era, ESR, NR3A1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

NBP1-41192

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-41192, RRID:AB_10005040

Product name

Mouse Monoclonal ER alpha/NR3A1 Antibody

Antibody type

Monoclonal

Description

Protein G purified. This antibody will recognize human ER alpha.

Reactivity

Human, Rabbit

Host

Mouse

Isotype

IgG

Vial size

100 ul

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ER alpha/NR3A1 Antibody (1F3) [NBP1-41192] - Whole cell extract (60 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ER alpha/NR3A1 Antibody (1F3) [NBP1-41192] - Sample (30 ug of whole cell lysate) A: MCF7 7. 5% SDS PAGE, antibody diluted at 1:500.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ER alpha/NR3A1 Antibody (1F3) [NBP1-41192] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody [1F3] diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ER alpha/NR3A1 Antibody (1F3) [NBP1-41192] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody diluted at 1:5000. HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.