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Western blot
Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- HPA000449 - Provider product page
- Provider
- Atlas Antibodies
- Proper citation
- Atlas Antibodies Cat#HPA000449, RRID:AB_1078753
- Product name
- Anti-ESR1
- Antibody type
- Polyclonal
- Description
- Polyclonal Antibody against Human ESR1, Gene description: estrogen receptor 1, Alternative Gene Names: Era, ESR, NR3A1, Validated applications: WB, IHC, ICC, Uniprot ID: P03372, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 0.1 mg/ml
- Storage
- Store at +4°C for short term storage. Long time storage is recommended at -20°C.
- Handling
- The antibody solution should be gently mixed before use.
Submitted references Alternative promoters and splicing create multiple functionally distinct isoforms of oestrogen receptor alpha in breast cancer and healthy tissues.
Epigenetic silencing of SALL 2 confers tamoxifen resistance in breast cancer
Antigen Masking During Fixation and Embedding, Dissected
Antibody performance in western blot applications is context‐dependent
Balcazar Lopez CE, Albrecht J, Hafstað V, Börjesson Freitag C, Vallon-Christersson J, Bellodi C, Persson H
Cancer medicine 2023 Sep;12(18):18931-18945
Cancer medicine 2023 Sep;12(18):18931-18945
Epigenetic silencing of SALL 2 confers tamoxifen resistance in breast cancer
Ye L, Lin C, Wang X, Li Q, Li Y, Wang M, Zhao Z, Wu X, Shi D, Xiao Y, Ren L, Jian Y, Yang M, Ou R, Deng G, Ouyang Y, Chen X, Li J, Song L
EMBO Molecular Medicine 2019;11(12)
EMBO Molecular Medicine 2019;11(12)
Antigen Masking During Fixation and Embedding, Dissected
Scalia C, Boi G, Bolognesi M, Riva L, Manzoni M, DeSmedt L, Bosisio F, Ronchi S, Leone B, Cattoretti G
Journal of Histochemistry & Cytochemistry 2016;65(1):5-20
Journal of Histochemistry & Cytochemistry 2016;65(1):5-20
Antibody performance in western blot applications is context‐dependent
Älgenäs C, Agaton C, Fagerberg L, Asplund A, Björling L, Björling E, Kampf C, Lundberg E, Nilsson P, Persson A, Wester K, Pontén F, Wernérus H, Uhlén M, Ottosson Takanen J, Hober S
Biotechnology Journal 2014;9(3):435-445
Biotechnology Journal 2014;9(3):435-445
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Enhanced validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Western blot analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-ESR1 antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
- Sample type
- Human
- Protocol
- Protocol
Supportive validation
- Submitted by
- Atlas Antibodies (provider)
- Enhanced method
- Orthogonal validation
- Main image
- Experimental details
- Immunohistochemistry analysis in human endometrium and cerebral cortex tissues using HPA000449 antibody. Corresponding ESR1 RNA-seq data are presented for the same tissues.
- Sample type
- Human
- Protocol
- Protocol
