Ab102356

antibody from Aladdin Scientific

Targeting: ESR1 ER-alpha, Era, ESR, NR3A1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

Ab102356

Provider

Aladdin Scientific

Product name

Recombinant Estrogen Receptor alpha Antibody

Antibody type

Recombinant

Description

Rabbit anti Human Estrogen Receptor alpha Antibody, Recombinant, could be used for WB, IHC, ICC/IF and so on.ApplicationWB: 1/500-1/1000 IHC: 1/50-1/100ICC/IF: 1/50-1/200 Protein FunctionNuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Can activate the transcriptional activity of TFF1.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

AA 98-116


Vial size

100_l,10_l,1ml,50_l

Concentration

2 mg/ml

Storage

Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Western blot

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Recombinant Estrogen Receptor alpha Antibody ( Ab102356 ) - Western Blot All lanes: Recombinant Estrogen Receptor alpha Antibody (Ab102356) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 66 kDa Observed band size: 66 kDa Exposure time: 30.2 s

Immunocytochemistry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Recombinant Estrogen Receptor alpha Antibody (Ab102356) - IF/ICC Immunocytochemistry analysis of Estrogen Receptor alpha (green) in MCF7 using Recombinant Estrogen Receptor alpha Antibody (Ab102356), and cells were counterstained with DAPI (blue).

Immunohistochemistry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Recombinant Estrogen Receptor alpha Antibody (Ab102356) - IHC Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Recombinant Estrogen Receptor alpha Antibody (Ab102356). High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.