Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [11]
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Validation data
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- Product number
- NBP1-84826 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-84826, RRID:AB_11016376
- Product name
- Rabbit Polyclonal ER alpha/NR3A1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human ER alpha/NR3A1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Antibody performance in western blot applications is context-dependent.
Algenäs C, Agaton C, Fagerberg L, Asplund A, Björling L, Björling E, Kampf C, Lundberg E, Nilsson P, Persson A, Wester K, Pontén F, Wernérus H, Uhlén M, Ottosson Takanen J, Hober S
Biotechnology journal 2014 Mar;9(3):435-45
Biotechnology journal 2014 Mar;9(3):435-45
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Western Blot: ER alpha/NR3A1 Antibody [NBP1-84826] - Analysis in control (vector only transfected HEK293T lysate) and ESR1 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Staining of human endometrium shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Staining of human rectum shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Staining of human colon using Anti-ESR1 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Staining of human cerebral cortex using Anti-ESR1 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Staining of human smooth muscle using Anti-ESR1 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Analysis in human endometrium and cerebral cortex tissues. Corresponding ER alpha/NR3A1 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Staining of human cerebral cortex shows no positivity in neurons as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Staining of human cerebral cortex, cervix, uterine, endometrium and gastrointestinal using Anti-ER alpha/NR3A1 antibody NBP1-84826 (A) shows similar protein distribution across tissues to independent antibody NBP1-84827 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Staining of human endometrium shows strong nuclear positivity in glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Staining of human small intestine shows no positivity in glandular cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ER alpha/NR3A1 Antibody [NBP1-84826] - Staining of human uterine cervix shows strong nuclear positivity in squamous epithelial cells.