- MA3-310 - Invitrogen Antibodies ESR1 antibody

Submitted references Raloxifene Stimulates Estrogen Signaling to Protect Against Age- and Sex-Related Intervertebral Disc Degeneration in Mice.

Bhadouria N, Berman AG, Wallace JM, Holguin N
Frontiers in bioengineering and biotechnology 2022;10:924918

Simultaneous defeat of MCF7 and MDA-MB-231 resistances by a hypericin PDT-tamoxifen hybrid therapy.

Theodossiou TA, Ali M, Grigalavicius M, Grallert B, Dillard P, Schink KO, Olsen CE, Wälchli S, Inderberg EM, Kubin A, Peng Q, Berg K
NPJ breast cancer 2019;5:13

High Estradiol Differentially Affects the Expression of the Glucose Transporter Type 4 in Pelvic Floor Muscles of Rats.

Carrasco-Ruiz MLÁ, Hernández-Aragón LG, Chávez-Ríos JR, Rodríguez-Antolín J, Pacheco P, Martínez-Gómez M, Cuevas-Romero E, Castelán F
International neurourology journal 2018 Sep;22(3):161-168

Role of Estrogens in the Size of Neuronal Somata of Paravaginal Ganglia in Ovariectomized Rabbits.

Hernández-Aragón LG, García-Villamar V, Carrasco-Ruiz ML, Nicolás-Toledo L, Ortega A, Cuevas-Romero E, Martínez-Gómez M, Castelán F
BioMed research international 2017;2017:2089645

Calmodulin-like protein 3 is an estrogen receptor alpha coregulator for gene expression and drug response in a SNP, estrogen, and SERM-dependent fashion.

Qin S, Ingle JN, Liu M, Yu J, Wickerham DL, Kubo M, Weinshilboum RM, Wang L
Breast cancer research : BCR 2017 Aug 18;19(1):95

Aromatase expression is linked to estrogenic sensitivity of periurethral muscles in female rabbits.

de los Ángeles Carrasco-Ruiz M, García-Villamar V, López-García K, Sánchez-García O, Pacheco P, Cuevas E, Martínez-Gómez M, Castelán F
Cell biochemistry and function 2015 Jun;33(4):188-95

Oestrogen upregulates L-type Ca²⁺ channels via oestrogen-receptor- by a regional genomic mechanism in female rabbit hearts.

Yang X, Chen G, Papp R, Defranco DB, Zeng F, Salama G
The Journal of physiology 2012 Feb 1;590(3):493-508

Immunodetection of nmt55/p54nrb isoforms in human breast cancer.

Pavao M, Huang YH, Hafer LJ, Moreland RB, Traish AM
BMC cancer 2001;1:15

Estradiol-induced phosphorylation of serine 118 in the estrogen receptor is independent of p42/p44 mitogen-activated protein kinase.

Joel PB, Traish AM, Lannigan DA
The Journal of biological chemistry 1998 May 22;273(21):13317-23

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Estrogen Receptor alpha (ER-alpha) (upper panel) using recombinant ER-alpha (Product # A15674) (lane 1), HeLa (lane 2), MCF7 (lane 3) and T-47D (lane 4) cell lysates (100 ng/lane) in reducing sample buffer (Product # 39000) and Page Ruler Plus Protein Ladder (Product # 26619) onto a Novex 4-20% Tris-HCl polyacrylamide gel (Product # WT4201BX10). Proteins were transferred to nitrocellulose membrane (Product # 88018) with Transfer Buffer (Product # 84731) using the G2 Fast Blotter (Product # 62288). Membrane was blocked in StartingBlock T20 (Product # 37543) for 30 minutes at room temperature. ER-alpha was detected at ~67 kD using an ER-alpha monoclonal antibody (Product # MA3-310) at a 1:1000 dilution in StartingBlock T20 overnight at at 4°C, followed by a goat anti-mouse IgG-HRP secondary antibody (Product # A28177) at a 1:5,000 dilution for 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product # 34076) and myECL Imager (Product # 62236). A loading control (lower panel) was performed by stripping the blot in Restore™ PLUS (Product # 46430) and reprobed with GAPDH antibody (Product # PA1-987) at a 1:1000 dilution in StartingBlock T20 for 1 hour at room temperature, followed by a goat anti-rabbit superclonal IgG-HRP secondary antibody (Product # A27036) at a 1:5000 dilution for 1 hour. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34078) and myECL Imager (Product # 62236).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Estrogen Receptor alpha using Anti-Estrogen Receptor alpha Monoclonal Antibody (EVG F9) (Product # MA3-310) shows staining in MCF-7 Cells. Estrogen Receptor alpha staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Estrogen Receptor alpha (Product # MA3-310) at a dilution of 1:200 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503, Goat Anti-Mouse). Images were taken at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Estrogen Receptor alpha using Anti-Estrogen Receptor alpha Monoclonal Antibody (EVG F9) (Product # MA3-310) shows staining in U251 Cells. Estrogen Receptor alpha staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Estrogen Receptor alpha (Product # MA3-310) at a dilution of 1:200 over night at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody (Product # 35503, Goat Anti-Mouse). Images were taken at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry was performed on cancer biopsies of deparaffinized Human breast carcinoma tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:20 with a mouse monoclonal antibody recognizing Estrogen Receptor alpha (Product # MA3-310) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry was performed on normal deparaffinized Human uterus tissue tissues. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:100 with a mouse monoclonal antibody recognizing Estrogen Receptor alpha (Product # MA3-310) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIGURE 1 Raloxifene increased ER-alpha protein expression in young and old IVDs, whereas OVX reduced ER-alpha protein expression. (A) Estrogen receptor-alpha (ER-alpha) immunostaining (arrow: presence of stain, arrowhead: absence of stain) in the (B) AF and (C) NP of young-adult (4 months), old (24 months), and OVX (6 months) mice. Data are represented as box plots with mean marked as cross (x), 25/75% deviation lines, and maximum/minimum whiskers. *: control (CON, n = 5/sex/group) vs. raloxifene (RAL); R: CON vs. RAL; S: male vs. female; RxS: interaction, vehicle (VEH, n = 8/group) vs. RAL, SHAM ( n = 4-5/group) vs. ovariectomized (OVX); # : aging effect (4 months vs. 6 months vs. 24 months), p < 0.05. AF, annulus fibrosus; NP, nucleus pulposus. Scale: 100 mum.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 2 CALML3 as a coregulator of ERalpha affects the expression of ZNF423 and BRCA1. a Interaction of ERalpha and CALML3 in ZNF423 rs9940645 WT or variant genotype breast cancer cells treated with 0.01 nM E2. Co-IP performed by immunoprecipitating the protein complex with anti-ERalpha antibody, followed by immunoblotting for CALML3, or vice versa. b - e CALML3 was knocked down in ERalpha + breast cancer cells homozygous for ZNF423 WT or variant SNP genotypes and followed by treatment with 0.01 nM E2 +- 4-OH-TAM/raloxifene 10 -7 mol/L. ZNF423 and BRCA1mRNA expression levels relative to beta-ACTN shown as the mean of three independent experiments (+- SEM). * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001 by two-tailed Student's t test. Protein levels determined by western blot ( WB ) analysis. IP immunoprecipitation, 4-OH-TAM 4-hydroxytamoxifene, E2 estradiol, ER alpha estrogen receptor alpha, Ral raloxifene

MA3-310

Antibody data