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Immunocytochemistry
Immunoprecipitation
Other assayAntibody data
- Antibody Data
- Antigen structure
- References [15]
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- Validations
- Other assay [2]
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- Product number
- MA5-12325 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-Estrogen Receptor alpha Monoclonal Antibody (AER314), Biotin
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-12328 targets Estrogen Receptor in WB, GS, and IP applications and shows reactivity with bovine, human, mouse, rabbit, and rat samples. By Western blot, MA5-12328 detects recombinant ER alpha, but may not be suitable for detection of endogenous protein. The MA5-12328 immunogen is purified, SDS-denatured calf uterus estrogen receptor (ER).
- Reactivity
- Human, Mouse, Rat, Bovine, Rabbit
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- AER314
- Vial size
- 500 µL
- Concentration
- 0.2 mg/ml
- Storage
- 4° C
Submitted references Characterization of estrogen response element binding proteins as biomarkers of breast cancer behavior.
Location analysis for the estrogen receptor-alpha reveals binding to diverse ERE sequences and widespread binding within repetitive DNA elements.
Expression and subcellular distribution of native estrogen receptor beta in murine C2C12 cells and skeletal muscle tissue.
Expression and localization of estrogen receptor alpha in the C2C12 murine skeletal muscle cell line.
Repressive domain of unliganded human estrogen receptor alpha associates with Hsc70.
Leukemogenic MLL fusion proteins bind across a broad region of the Hox a9 locus, promoting transcription and multiple histone modifications.
Nuclear receptor target gene discovery using high-throughput chromatin immunoprecipitation.
Identification of estrogen receptor beta expression in Chinese hamster ovary (CHO) cells and comparison of estrogen-responsive gene transcription in cells adapted to serum-free media.
The effects of estrogen, its antagonist ICI 182, 780, and interferon-tau on the expression of estrogen receptors and integrin alphaV beta 3 on cycle day 16 in bovine endometrium.
Nuclear receptor function requires a TFTC-type histone acetyl transferase complex.
Occurrence of estrogen receptor alpha in bovine placentomes throughout mid and late gestation and at parturition.
Differential cellular localization of estrogen receptor alpha in uterine and mammary cells.
Affinity chromatography of human estrogen receptor-alpha expressed in Saccharomyces cerevisiae. Combination of heparin- and 17beta-estradiol-affinity chromatography.
Classification of breast cancer cells on the basis of a functional assay for estrogen receptor.
Identification of a variant estrogen receptor lacking exon 4 and its coexpression with wild-type estrogen receptor in ovarian carcinomas.
Kruer TL, Cummins TD, Powell DW, Wittliff JL
Clinical biochemistry 2013 Nov;46(16-17):1739-46
Clinical biochemistry 2013 Nov;46(16-17):1739-46
Location analysis for the estrogen receptor-alpha reveals binding to diverse ERE sequences and widespread binding within repetitive DNA elements.
Mason CE, Shu FJ, Wang C, Session RM, Kallen RG, Sidell N, Yu T, Liu MH, Cheung E, Kallen CB
Nucleic acids research 2010 Apr;38(7):2355-68
Nucleic acids research 2010 Apr;38(7):2355-68
Expression and subcellular distribution of native estrogen receptor beta in murine C2C12 cells and skeletal muscle tissue.
Milanesi L, Vasconsuelo A, de Boland AR, Boland R
Steroids 2009 Jun;74(6):489-97
Steroids 2009 Jun;74(6):489-97
Expression and localization of estrogen receptor alpha in the C2C12 murine skeletal muscle cell line.
Milanesi L, Russo de Boland A, Boland R
Journal of cellular biochemistry 2008 Jul 1;104(4):1254-73
Journal of cellular biochemistry 2008 Jul 1;104(4):1254-73
Repressive domain of unliganded human estrogen receptor alpha associates with Hsc70.
Ogawa S, Oishi H, Mezaki Y, Kouzu-Fujita M, Matsuyama R, Nakagomi M, Mori E, Murayama E, Nagasawa H, Kitagawa H, Yanagisawa J, Yano T, Kato S
Genes to cells : devoted to molecular & cellular mechanisms 2005 Dec;10(12):1095-102
Genes to cells : devoted to molecular & cellular mechanisms 2005 Dec;10(12):1095-102
Leukemogenic MLL fusion proteins bind across a broad region of the Hox a9 locus, promoting transcription and multiple histone modifications.
Milne TA, Martin ME, Brock HW, Slany RK, Hess JL
Cancer research 2005 Dec 15;65(24):11367-74
Cancer research 2005 Dec 15;65(24):11367-74
Nuclear receptor target gene discovery using high-throughput chromatin immunoprecipitation.
Laganière J, Deblois G, Giguère V
Methods in enzymology 2003;364:339-50
Methods in enzymology 2003;364:339-50
Identification of estrogen receptor beta expression in Chinese hamster ovary (CHO) cells and comparison of estrogen-responsive gene transcription in cells adapted to serum-free media.
Thomas PB, Risinger KE, Klinge CM
The Journal of steroid biochemistry and molecular biology 2003 Jul;86(1):41-55
The Journal of steroid biochemistry and molecular biology 2003 Jul;86(1):41-55
The effects of estrogen, its antagonist ICI 182, 780, and interferon-tau on the expression of estrogen receptors and integrin alphaV beta 3 on cycle day 16 in bovine endometrium.
Kimmins S, Russell GL, Lim HC, Hall BK, MacLaren LA
Reproductive biology and endocrinology : RB&E 2003 Apr 24;1:38
Reproductive biology and endocrinology : RB&E 2003 Apr 24;1:38
Nuclear receptor function requires a TFTC-type histone acetyl transferase complex.
Yanagisawa J, Kitagawa H, Yanagida M, Wada O, Ogawa S, Nakagomi M, Oishi H, Yamamoto Y, Nagasawa H, McMahon SB, Cole MD, Tora L, Takahashi N, Kato S
Molecular cell 2002 Mar;9(3):553-62
Molecular cell 2002 Mar;9(3):553-62
Occurrence of estrogen receptor alpha in bovine placentomes throughout mid and late gestation and at parturition.
Schuler G, Wirth C, Teichmann U, Failing K, Leiser R, Thole H, Hoffmann B
Biology of reproduction 2002 Apr;66(4):976-82
Biology of reproduction 2002 Apr;66(4):976-82
Differential cellular localization of estrogen receptor alpha in uterine and mammary cells.
Monje P, Zanello S, Holick M, Boland R
Molecular and cellular endocrinology 2001 Jul 5;181(1-2):117-29
Molecular and cellular endocrinology 2001 Jul 5;181(1-2):117-29
Affinity chromatography of human estrogen receptor-alpha expressed in Saccharomyces cerevisiae. Combination of heparin- and 17beta-estradiol-affinity chromatography.
Feng W, Graumann K, Hahn R, Jungbauer A
Journal of chromatography. A 1999 Aug 6;852(1):161-73
Journal of chromatography. A 1999 Aug 6;852(1):161-73
Classification of breast cancer cells on the basis of a functional assay for estrogen receptor.
Biswas DK, Averboukh L, Sheng S, Martin K, Ewaniuk DS, Jawde TF, Wang F, Pardee AB
Molecular medicine (Cambridge, Mass.) 1998 Jul;4(7):454-67
Molecular medicine (Cambridge, Mass.) 1998 Jul;4(7):454-67
Identification of a variant estrogen receptor lacking exon 4 and its coexpression with wild-type estrogen receptor in ovarian carcinomas.
Park W, Choi JJ, Hwang ES, Lee JH
Clinical cancer research : an official journal of the American Association for Cancer Research 1996 Dec;2(12):2029-35
Clinical cancer research : an official journal of the American Association for Cancer Research 1996 Dec;2(12):2029-35
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Estrogen Receptor using Estrogen Receptor Monoclonal Antibody (Product # MA5-12325) on Native Human T47D Cells.
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Estrogen Receptor using Estrogen Receptor Monoclonal Antibody (Product # MA5-12325) on denatured Human MCF-7 Cells.
- Conjugate
- Biotin
