MA5-12328

antibody from Invitrogen Antibodies

Targeting: ESR1 ER-alpha, Era, ESR, NR3A1

Provider product page for MA5-12328

Western blot

Other assay

Antibody data

Antibody Data
Antigen structure
References [24]
Comments [0]
Validations
Western blot [1]
Other assay [2]

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Product number: MA5-12328 - Provider product page
Provider: Invitrogen Antibodies
Product name: Estrogen Receptor alpha Monoclonal Antibody (AER314)
Antibody type: Monoclonal
Antigen: Purifed from natural sources
Description: MA5-12328 targets Estrogen Receptor in WB, GS, and IP applications and shows reactivity with bovine, human, mouse, rabbit, and rat samples. By Western blot, MA5-12328 detects recombinant ER alpha, but may not be suitable for detection of endogenous protein.
Antibody clone number: AER314
Concentration: 0.2 mg/mL

ESR1 protein structure - MA5-12328 shown in red.

Submitted references Targeting BIG3-PHB2 interaction to overcome tamoxifen resistance in breast cancer cells.

Yoshimaru T, Komatsu M, Matsuo T, Chen YA, Murakami Y, Mizuguchi K, Mizohata E, Inoue T, Akiyama M, Yamaguchi R, Imoto S, Miyano S, Miyoshi Y, Sasa M, Nakamura Y, Katagiri T
Nature communications 2013;4:2443

Characterization of estrogen response element binding proteins as biomarkers of breast cancer behavior.

Kruer TL, Cummins TD, Powell DW, Wittliff JL
Clinical biochemistry 2013 Nov;46(16-17):1739-46

Location analysis for the estrogen receptor-alpha reveals binding to diverse ERE sequences and widespread binding within repetitive DNA elements.

Mason CE, Shu FJ, Wang C, Session RM, Kallen RG, Sidell N, Yu T, Liu MH, Cheung E, Kallen CB
Nucleic acids research 2010 Apr;38(7):2355-68

Expression and subcellular distribution of native estrogen receptor beta in murine C2C12 cells and skeletal muscle tissue.

Milanesi L, Vasconsuelo A, de Boland AR, Boland R
Steroids 2009 Jun;74(6):489-97

Expression and localization of estrogen receptor alpha in the C2C12 murine skeletal muscle cell line.

Milanesi L, Russo de Boland A, Boland R
Journal of cellular biochemistry 2008 Jul 1;104(4):1254-73

Repressive domain of unliganded human estrogen receptor alpha associates with Hsc70.

Ogawa S, Oishi H, Mezaki Y, Kouzu-Fujita M, Matsuyama R, Nakagomi M, Mori E, Murayama E, Nagasawa H, Kitagawa H, Yanagisawa J, Yano T, Kato S
Genes to cells : devoted to molecular & cellular mechanisms 2005 Dec;10(12):1095-102

Leukemogenic MLL fusion proteins bind across a broad region of the Hox a9 locus, promoting transcription and multiple histone modifications.

Milne TA, Martin ME, Brock HW, Slany RK, Hess JL
Cancer research 2005 Dec 15;65(24):11367-74

Interplay between estrogen response element sequence and ligands controls in vivo binding of estrogen receptor to regulated genes.

Krieg AJ, Krieg SA, Ahn BS, Shapiro DJ
The Journal of biological chemistry 2004 Feb 6;279(6):5025-34

Nuclear receptor target gene discovery using high-throughput chromatin immunoprecipitation.

Laganière J, Deblois G, Giguère V
Methods in enzymology 2003;364:339-50

Nuclear receptor target gene discovery using high-throughput chromatin immunoprecipitation.

Laganière J, Deblois G, Giguère V
Methods in enzymology 2003;364:339-50

Identification of estrogen receptor beta expression in Chinese hamster ovary (CHO) cells and comparison of estrogen-responsive gene transcription in cells adapted to serum-free media.

Thomas PB, Risinger KE, Klinge CM
The Journal of steroid biochemistry and molecular biology 2003 Jul;86(1):41-55

Identification of estrogen receptor beta expression in Chinese hamster ovary (CHO) cells and comparison of estrogen-responsive gene transcription in cells adapted to serum-free media.

Thomas PB, Risinger KE, Klinge CM
The Journal of steroid biochemistry and molecular biology 2003 Jul;86(1):41-55

The effects of estrogen, its antagonist ICI 182, 780, and interferon-tau on the expression of estrogen receptors and integrin alphaV beta 3 on cycle day 16 in bovine endometrium.

Kimmins S, Russell GL, Lim HC, Hall BK, MacLaren LA
Reproductive biology and endocrinology : RB&E 2003 Apr 24;1:38

The effects of estrogen, its antagonist ICI 182, 780, and interferon-tau on the expression of estrogen receptors and integrin alphaV beta 3 on cycle day 16 in bovine endometrium.

Kimmins S, Russell GL, Lim HC, Hall BK, MacLaren LA
Reproductive biology and endocrinology : RB&E 2003 Apr 24;1:38

Nuclear receptor function requires a TFTC-type histone acetyl transferase complex.

Yanagisawa J, Kitagawa H, Yanagida M, Wada O, Ogawa S, Nakagomi M, Oishi H, Yamamoto Y, Nagasawa H, McMahon SB, Cole MD, Tora L, Takahashi N, Kato S
Molecular cell 2002 Mar;9(3):553-62

Occurrence of estrogen receptor alpha in bovine placentomes throughout mid and late gestation and at parturition.

Schuler G, Wirth C, Teichmann U, Failing K, Leiser R, Thole H, Hoffmann B
Biology of reproduction 2002 Apr;66(4):976-82

Occurrence of estrogen receptor alpha in bovine placentomes throughout mid and late gestation and at parturition.

Schuler G, Wirth C, Teichmann U, Failing K, Leiser R, Thole H, Hoffmann B
Biology of reproduction 2002 Apr;66(4):976-82

Differential cellular localization of estrogen receptor alpha in uterine and mammary cells.

Monje P, Zanello S, Holick M, Boland R
Molecular and cellular endocrinology 2001 Jul 5;181(1-2):117-29

Regulation of the functional interaction between cyclin D1 and the estrogen receptor.

Lamb J, Ladha MH, McMahon C, Sutherland RL, Ewen ME
Molecular and cellular biology 2000 Dec;20(23):8667-75

P/CAF associates with cyclin D1 and potentiates its activation of the estrogen receptor.

McMahon C, Suthiphongchai T, DiRenzo J, Ewen ME
Proceedings of the National Academy of Sciences of the United States of America 1999 May 11;96(10):5382-7

Affinity chromatography of human estrogen receptor-alpha expressed in Saccharomyces cerevisiae. Combination of heparin- and 17beta-estradiol-affinity chromatography.

Feng W, Graumann K, Hahn R, Jungbauer A
Journal of chromatography. A 1999 Aug 6;852(1):161-73

Affinity chromatography of human estrogen receptor-alpha expressed in Saccharomyces cerevisiae. Combination of heparin- and 17beta-estradiol-affinity chromatography.

Feng W, Graumann K, Hahn R, Jungbauer A
Journal of chromatography. A 1999 Aug 6;852(1):161-73

Classification of breast cancer cells on the basis of a functional assay for estrogen receptor.

Biswas DK, Averboukh L, Sheng S, Martin K, Ewaniuk DS, Jawde TF, Wang F, Pardee AB
Molecular medicine (Cambridge, Mass.) 1998 Jul;4(7):454-67

Identification of a variant estrogen receptor lacking exon 4 and its coexpression with wild-type estrogen receptor in ovarian carcinomas.

Park W, Choi JJ, Hwang ES, Lee JH
Clinical cancer research : an official journal of the American Association for Cancer Research 1996 Dec;2(12):2029-35

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Estrogen Receptor (ER) was performed by loading the indicated amounts of recombinant full-length human ER alpha (Product # A15674) and ER beta (Product # A15664) proteins, and 10 µL of PageRuler Prestained Protein Ladder (Product # 26616) per well onto a Novex® 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a PVDF membrane (Product # 88518) using the G2 Blotter (Product # 62288), and blocked with 5% milk in TBST for at least 1 hour at room temperature. ER alpha was detected at ~66 kD (left panel) using an ER monoclonal antibody (Product # MA5-12328) at a dilution of 1:200 in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-mouse IgG secondary antibody (Product # 31430) at a dilution of 1:40,000. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080). The blot was then stripped with Restore Plus Western blot stripping buffer (Product # 46430) for at least 15 minutes at room temperature, re-blocked with 5% milk in TBST for at least 1 hour at room temperature, and re-probed with an ER beta polyclonal antibody (Product # PA1-313, right panel) at a dilution of 1:2000 overnight at 4C, followed by an HRP-conjugated goat anti-rabbit IgG secondary antibody (Product # 31460) at a dilution of 1:40,000. Chemiluminescent detection was again performed using SuperSignal West Pico (Product # 34080).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunoprecipitation of Estrogen Receptor using Estrogen Receptor Monoclonal Antibody (Product # MA5-12328) on Native Human T47D Cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunoprecipitation of Estrogen Receptor using Estrogen Receptor Monoclonal Antibody (Product # MA5-12328) on denatured Human MCF-7 Cells.