MA5-13062

antibody from Invitrogen Antibodies

Targeting: ESR1 ER-alpha, Era, ESR, NR3A1

Provider product page for MA5-13062

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Antibody Data
Antigen structure
References [61]
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Product number: MA5-13062 - Provider product page
Provider: Invitrogen Antibodies
Product name: Anti-Estrogen Receptor alpha Monoclonal Antibody (TE111.5D11), Biotin
Antibody type: Monoclonal
Antigen: Other
Description: MA5-13065 targets Estrogen Receptor in ChIP, GS, IP, and WB applications and shows reactivity with Bovine, Canine, Chicken, Hamster, Human, mouse, Ovine, Porcine, Rabbit, and Rat samples. The TE111.5D11 clone was developed against the entire ER ligand binding domain (LBD), but has greatest affinity for ER-alpha. Because ER-alpha and ER-beta share 5060% homology, MA5-13065 recognizes ER-beta1 with lower affinity. The MA5-13065 immunogen is 35kDa, C-terminal fragment (aa 302-595) of human ER expressed in E. coli.
Reactivity: Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Hamster, Porcine, Rabbit
Host: Mouse
Conjugate: Biotin
Isotype: IgG
Antibody clone number: TE111.5D11
Vial size: 500 µL
Concentration: 0.2 mg/ml
Storage: 4° C

ESR1 protein structure - MA5-13062 shown in red.

Submitted references Changes in the cellular localization of estrogen receptor alpha in the growing and regressing ovaries of Gallus domesticus during development.

González-Morán MG
Biochemical and biophysical research communications 2014 Apr 25;447(1):197-204

Early histone modifications in the ventromedial hypothalamus and preoptic area following oestradiol administration.

Gagnidze K, Weil ZM, Faustino LC, Schaafsma SM, Pfaff DW
Journal of neuroendocrinology 2013 Oct;25(10):939-55

Characterization of estrogen response element binding proteins as biomarkers of breast cancer behavior.

Kruer TL, Cummins TD, Powell DW, Wittliff JL
Clinical biochemistry 2013 Nov;46(16-17):1739-46

MLL-ENL inhibits polycomb repressive complex 1 to achieve efficient transformation of hematopoietic cells.

Maethner E, Garcia-Cuellar MP, Breitinger C, Takacova S, Divoky V, Hess JL, Slany RK
Cell reports 2013 May 30;3(5):1553-66

Interplay between estrogen receptor and AKT in estradiol-induced alternative splicing.

Bhat-Nakshatri P, Song EK, Collins NR, Uversky VN, Dunker AK, O'Malley BW, Geistlinger TR, Carroll JS, Brown M, Nakshatri H
BMC medical genomics 2013 Jun 11;6:21

Estrogen receptor α L543A,L544A mutation changes antagonists to agonists, correlating with the ligand binding domain dimerization associated with DNA binding activity.

Arao Y, Hamilton KJ, Coons LA, Korach KS
The Journal of biological chemistry 2013 Jul 19;288(29):21105-16

Reprogramming the chromatin landscape: interplay of the estrogen and glucocorticoid receptors at the genomic level.

Miranda TB, Voss TC, Sung MH, Baek S, John S, Hawkins M, Grøntved L, Schiltz RL, Hager GL
Cancer research 2013 Aug 15;73(16):5130-9

Localization of estrogen receptor in the central lymphoid organs of chickens during the late stage of embryogenesis.

Katayama M, Fukuda T, Narabara K, Abe A, Kondo Y
Bioscience, biotechnology, and biochemistry 2012;76(11):2003-7

Generation of NSE-MerCreMer transgenic mice with tamoxifen inducible Cre activity in neurons.

Kam MK, Lee KY, Tam PK, Lui VC
PloS one 2012;7(5):e35799

Novel senescence associated gene, YPEL3, is repressed by estrogen in ER+ mammary tumor cells and required for tamoxifen-induced cellular senescence.

Tuttle R, Miller KR, Maiorano JN, Termuhlen PM, Gao Y, Berberich SJ
International journal of cancer 2012 May 15;130(10):2291-9

Differential DNase I hypersensitivity reveals factor-dependent chromatin dynamics.

He HH, Meyer CA, Chen MW, Jordan VC, Brown M, Liu XS
Genome research 2012 Jun;22(6):1015-25

Integrated genome-wide chromatin occupancy and expression analyses identify key myeloid pro-differentiation transcription factors repressed by Myb.

Zhao L, Glazov EA, Pattabiraman DR, Al-Owaidi F, Zhang P, Brown MA, Leo PJ, Gonda TJ
Nucleic acids research 2011 Jun;39(11):4664-79

GATA4 regulates estrogen receptor-alpha-mediated osteoblast transcription.

Miranda-Carboni GA, Guemes M, Bailey S, Anaya E, Corselli M, Peault B, Krum SA
Molecular endocrinology (Baltimore, Md.) 2011 Jul;25(7):1126-36

Subcellular localization of activated AKT in estrogen receptor- and progesterone receptor-expressing breast cancers: potential clinical implications.

Badve S, Collins NR, Bhat-Nakshatri P, Turbin D, Leung S, Thorat M, Dunn SE, Geistlinger TR, Carroll JS, Brown M, Bose S, Teitell MA, Nakshatri H
The American journal of pathology 2010 May;176(5):2139-49

The tri-nucleotide spacer sequence between estrogen response element half-sites is conserved and modulates ERalpha-mediated transcriptional responses.

Shu FJ, Sidell N, Yang D, Kallen CB
The Journal of steroid biochemistry and molecular biology 2010 Jun;120(4-5):172-9

Estrogen-dependent transcription of the NEL-like 2 (NELL2) gene and its role in protection from cell death.

Choi EJ, Kim DH, Kim JG, Kim DY, Kim JD, Seol OJ, Jeong CS, Park JW, Choi MY, Kang SG, Costa ME, Ojeda SR, Lee BJ
The Journal of biological chemistry 2010 Aug 6;285(32):25074-84

Location analysis for the estrogen receptor-alpha reveals binding to diverse ERE sequences and widespread binding within repetitive DNA elements.

Mason CE, Shu FJ, Wang C, Session RM, Kallen RG, Sidell N, Yu T, Liu MH, Cheung E, Kallen CB
Nucleic acids research 2010 Apr;38(7):2355-68

Differential actions of estrogen and SERMs in regulation of the actin cytoskeleton of endometrial cells.

Flamini MI, Sanchez AM, Goglia L, Tosi V, Genazzani AR, Simoncini T
Molecular human reproduction 2009 Oct;15(10):675-85

Catechol-o-methyltransferase expression and 2-methoxyestradiol affect microtubule dynamics and modify steroid receptor signaling in leiomyoma cells.

Salama SA, Kamel MW, Botting S, Salih SM, Borahay MA, Hamed AA, Kilic GS, Saeed M, Williams MY, Diaz-Arrastia CR
PloS one 2009 Oct 7;4(10):e7356

Expression and subcellular distribution of native estrogen receptor beta in murine C2C12 cells and skeletal muscle tissue.

Milanesi L, Vasconsuelo A, de Boland AR, Boland R
Steroids 2009 Jun;74(6):489-97

Coactivator function defines the active estrogen receptor alpha cistrome.

Lupien M, Eeckhoute J, Meyer CA, Krum SA, Rhodes DR, Liu XS, Brown M
Molecular and cellular biology 2009 Jun;29(12):3413-23

EKLF/KLF1 controls cell cycle entry via direct regulation of E2f2.

Tallack MR, Keys JR, Humbert PO, Perkins AC
The Journal of biological chemistry 2009 Jul 31;284(31):20966-74

Two estrogen response element sequences near the PCNA gene are not responsible for its estrogen-enhanced expression in MCF7 cells.

Wang C, Yu J, Kallen CB
PloS one 2008;3(10):e3523

Extra-nuclear signalling of estrogen receptor to breast cancer cytoskeletal remodelling, migration and invasion.

Giretti MS, Fu XD, De Rosa G, Sarotto I, Baldacci C, Garibaldi S, Mannella P, Biglia N, Sismondi P, Genazzani AR, Simoncini T
PloS one 2008 May 21;3(5):e2238

Expression and localization of estrogen receptor alpha in the C2C12 murine skeletal muscle cell line.

Milanesi L, Russo de Boland A, Boland R
Journal of cellular biochemistry 2008 Jul 1;104(4):1254-73

Estrogen protects bone by inducing Fas ligand in osteoblasts to regulate osteoclast survival.

Krum SA, Miranda-Carboni GA, Hauschka PV, Carroll JS, Lane TF, Freedman LP, Brown M
The EMBO journal 2008 Feb 6;27(3):535-45

Estrogen-induced activation of hypoxia-inducible factor-1alpha, vascular endothelial growth factor expression, and edema in the uterus are mediated by the phosphatidylinositol 3-kinase/Akt pathway.

Kazi AA, Koos RD
Endocrinology 2007 May;148(5):2363-74

Erythroid Kruppel-like factor regulates the G1 cyclin dependent kinase inhibitor p18INK4c.

Tallack MR, Keys JR, Perkins AC
Journal of molecular biology 2007 Jun 1;369(2):313-21

Interplay between estrogen-related receptor alpha (ERRalpha) and gamma (ERRgamma) on the regulation of ERRalpha gene expression.

Zhang Z, Teng CT
Molecular and cellular endocrinology 2007 Jan 29;264(1-2):128-41

Interaction of MLL amino terminal sequences with menin is required for transformation.

Caslini C, Yang Z, El-Osta M, Milne TA, Slany RK, Hess JL
Cancer research 2007 Aug 1;67(15):7275-83

Physiologically high concentrations of 17beta-estradiol enhance NF-kappaB activity in human T cells.

Hirano S, Furutama D, Hanafusa T
American journal of physiology. Regulatory, integrative and comparative physiology 2007 Apr;292(4):R1465-71

Estrogen receptor protein interaction with phosphatidylinositol 3-kinase leads to activation of phosphorylated Akt and extracellular signal-regulated kinase 1/2 in the same population of cortical neurons: a unified mechanism of estrogen action.

Mannella P, Brinton RD
The Journal of neuroscience : the official journal of the Society for Neuroscience 2006 Sep 13;26(37):9439-47

Genome-wide analysis of estrogen receptor binding sites.

Carroll JS, Meyer CA, Song J, Li W, Geistlinger TR, Eeckhoute J, Brodsky AS, Keeton EK, Fertuck KC, Hall GF, Wang Q, Bekiranov S, Sementchenko V, Fox EA, Silver PA, Gingeras TR, Liu XS, Brown M
Nature genetics 2006 Nov;38(11):1289-97

Vascular endothelial growth factor restores erectile function through modulation of the insulin-like growth factor system and sex hormone receptors in diabetic rat.

Shirai M, Yamanaka M, Shiina H, Igawa M, Kawakami T, Ishii N, Lue TF, Fujime M, Dahiya R
Biochemical and biophysical research communications 2006 Mar 17;341(3):755-62

Identification of estrogen receptor ligands leading to activation of non-genomic signaling pathways while exhibiting only weak transcriptional activity.

Wessler S, Otto C, Wilck N, Stangl V, Fritzemeier KH
The Journal of steroid biochemistry and molecular biology 2006 Jan;98(1):25-35

Combinatorial analysis of transcription factor partners reveals recruitment of c-MYC to estrogen receptor-alpha responsive promoters.

Cheng AS, Jin VX, Fan M, Smith LT, Liyanarachchi S, Yan PS, Leu YW, Chan MW, Plass C, Nephew KP, Davuluri RV, Huang TH
Molecular cell 2006 Feb 3;21(3):393-404

Estrogen receptor alpha interacts with Galpha13 to drive actin remodeling and endothelial cell migration via the RhoA/Rho kinase/moesin pathway.

Simoncini T, Scorticati C, Mannella P, Fadiel A, Giretti MS, Fu XD, Baldacci C, Garibaldi S, Caruso A, Fornari L, Naftolin F, Genazzani AR
Molecular endocrinology (Baltimore, Md.) 2006 Aug;20(8):1756-71

An estrogen receptor-alpha/p300 complex activates the BRCA-1 promoter at an AP-1 site that binds Jun/Fos transcription factors: repressive effects of p53 on BRCA-1 transcription.

Jeffy BD, Hockings JK, Kemp MQ, Morgan SS, Hager JA, Beliakoff J, Whitesell LJ, Bowden GT, Romagnolo DF
Neoplasia (New York, N.Y.) 2005 Sep;7(9):873-82

Sumoylation of the estrogen receptor alpha hinge region regulates its transcriptional activity.

Sentis S, Le Romancer M, Bianchin C, Rostan MC, Corbo L
Molecular endocrinology (Baltimore, Md.) 2005 Nov;19(11):2671-84

Chromosome-wide mapping of estrogen receptor binding reveals long-range regulation requiring the forkhead protein FoxA1.

Carroll JS, Liu XS, Brodsky AS, Li W, Meyer CA, Szary AJ, Eeckhoute J, Shao W, Hestermann EV, Geistlinger TR, Fox EA, Silver PA, Brown M
Cell 2005 Jul 15;122(1):33-43

Leukemogenic MLL fusion proteins bind across a broad region of the Hox a9 locus, promoting transcription and multiple histone modifications.

Milne TA, Martin ME, Brock HW, Slany RK, Hess JL
Cancer research 2005 Dec 15;65(24):11367-74

Formation of an IKKalpha-dependent transcription complex is required for estrogen receptor-mediated gene activation.

Park KJ, Krishnan V, O'Malley BW, Yamamoto Y, Gaynor RB
Molecular cell 2005 Apr 1;18(1):71-82

Estrogen response element and the promoter context of the human and mouse lactoferrin genes influence estrogen receptor alpha-mediated transactivation activity in mammary gland cells.

Stokes K, Alston-Mills B, Teng C
Journal of molecular endocrinology 2004 Oct;33(2):315-34

Inhibition of serum-stimulated mitogen activated protein kinase by 1alpha,25(OH)2-vitamin D3 in MCF-7 breast cancer cells.

Capiati DA, Rossi AM, Picotto G, Benassati S, Boland RL
Journal of cellular biochemistry 2004 Oct 1;93(2):384-97

Overexpression of the colony-stimulating factor (CSF-1) and/or its receptor c-fms in mammary glands of transgenic mice results in hyperplasia and tumor formation.

Kirma N, Luthra R, Jones J, Liu YG, Nair HB, Mandava U, Tekmal RR
Cancer research 2004 Jun 15;64(12):4162-70

Context-dependent regulation of GATA-1 by friend of GATA-1.

Letting DL, Chen YY, Rakowski C, Reedy S, Blobel GA
Proceedings of the National Academy of Sciences of the United States of America 2004 Jan 13;101(2):476-81

Molecular mechanism of estrogen receptor (ER)alpha-specific, estradiol-dependent expression of the progesterone receptor (PR) B-isoform.

Flötotto T, Niederacher D, Hohmann D, Heimerzheim T, Dall P, Djahansouzi S, Bender HG, Hanstein B
The Journal of steroid biochemistry and molecular biology 2004 Feb;88(2):131-42

Molecular basis of cell membrane estrogen receptor interaction with phosphatidylinositol 3-kinase in endothelial cells.

Simoncini T, Rabkin E, Liao JK
Arteriosclerosis, thrombosis, and vascular biology 2003 Feb 1;23(2):198-203

Estrogen receptor expression in laryngeal muscle in relation to estrogen-dependent increases in synapse strength.

Wu KH, Tobias ML, Kelley DB
Neuroendocrinology 2003 Aug;78(2):72-80

Growth factor requirements and basal phenotype of an immortalized mammary epithelial cell line.

DiRenzo J, Signoretti S, Nakamura N, Rivera-Gonzalez R, Sellers W, Loda M, Brown M
Cancer research 2002 Jan 1;62(1):89-98

Progesterone receptor isoforms expression pattern in human astrocytomas.

González-Agüero G, Ondarza R, Gamboa-Domínguez A, Cerbón MA, Camacho-Arroyo I
Brain research bulletin 2001 Sep 1;56(1):43-8

Ligand-independent activation of oestrogen receptor alpha by caveolin-1.

Schlegel A, Wang C, Pestell RG, Lisanti MP
The Biochemical journal 2001 Oct 1;359(Pt 1):203-10

Overexpression of aromatase leads to hyperplasia and changes in the expression of genes involved in apoptosis, cell cycle, growth, and tumor suppressor functions in the mammary glands of transgenic mice.

Kirma N, Gill K, Mandava U, Tekmal RR
Cancer research 2001 Mar 1;61(5):1910-8

Differential cellular localization of estrogen receptor alpha in uterine and mammary cells.

Monje P, Zanello S, Holick M, Boland R
Molecular and cellular endocrinology 2001 Jul 5;181(1-2):117-29

A therapeutic target for hormone-independent estrogen receptor-positive breast cancers.

Biswas DK, Cruz A, Pettit N, Mutter GL, Pardee AB
Molecular medicine (Cambridge, Mass.) 2001 Jan;7(1):59-67

Role of estrogen receptor ligand and estrogen response element sequence on interaction with chicken ovalbumin upstream promoter transcription factor (COUP-TF).

Klinge CM
The Journal of steroid biochemistry and molecular biology 1999 Nov;71(1-2):1-19

17 Beta-estradiol increases VEGF receptor-2 and promotes DNA synthesis in retinal microvascular endothelial cells.

Suzuma I, Mandai M, Takagi H, Suzuma K, Otani A, Oh H, Kobayashi K, Honda Y
Investigative ophthalmology & visual science 1999 Aug;40(9):2122-9

Ligand-independent recruitment of steroid receptor coactivators to estrogen receptor by cyclin D1.

Zwijsen RM, Buckle RS, Hijmans EM, Loomans CJ, Bernards R
Genes & development 1998 Nov 15;12(22):3488-98

Classification of breast cancer cells on the basis of a functional assay for estrogen receptor.

Biswas DK, Averboukh L, Sheng S, Martin K, Ewaniuk DS, Jawde TF, Wang F, Pardee AB
Molecular medicine (Cambridge, Mass.) 1998 Jul;4(7):454-67

Calmodulin is essential for estrogen receptor interaction with its motif and activation of responsive promoter.

Biswas DK, Reddy PV, Pickard M, Makkad B, Pettit N, Pardee AB
The Journal of biological chemistry 1998 Dec 11;273(50):33817-24

Cyclin D1 stimulation of estrogen receptor transcriptional activity independent of cdk4.

Neuman E, Ladha MH, Lin N, Upton TM, Miller SJ, DiRenzo J, Pestell RG, Hinds PW, Dowdy SF, Brown M, Ewen ME
Molecular and cellular biology 1997 Sep;17(9):5338-47

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunoprecipitation of Estrogen Receptor using Estrogen Receptor Monoclonal Antibody (Product # MA5-13062) on Native Human MCF-7 Cells.
Conjugate: Biotin

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunoprecipitation of Estrogen Receptor using Estrogen Receptor Monoclonal Antibody (Product # MA5-13062) on denatured Human MCF-7 Cells.
Conjugate: Biotin