MA5-13191

antibody from Invitrogen Antibodies

Targeting: ESR1 ER-alpha, Era, ESR, NR3A1

Provider product page for MA5-13191

Western blot

Immunocytochemistry

Immunohistochemistry

Other assay

Antibody data

Antibody Data
Antigen structure
References [81]
Comments [0]
Validations
Western blot [2]
Immunocytochemistry [1]
Immunohistochemistry [1]
Other assay [10]

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Product number: MA5-13191 - Provider product page
Provider: Invitrogen Antibodies
Product name: Estrogen Receptor alpha Monoclonal Antibody (1D5)
Antibody type: Monoclonal
Antigen: Recombinant full-length protein
Description: Staining of formalin-fixed, paraffin-embedded tissues requires boiling tissues in 10mM citrate buffer (pH 6.0) for 10-20 min, followed by cooling at room temperature for 20 min. A recommended positive control is MCF-7 cells. For optimal IF staining, it is recommended to fix and permeabilize cells with methanol.
Reactivity: Human
Host: Mouse
Isotype: IgG
Antibody clone number: 1D5
Vial size: 500 µL
Concentration: 0.8 mg/mL
Storage: 4° C

ESR1 protein structure - MA5-13191 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 Loss of tissue integrity and leukocyte infiltration after mating in the absence of vaginal epithelial ESR1. Immunohistochemical (IHC) staining of ESR1, H&E, Ki67 IHC, Masson's Trichrome (MT), and Periodic Acid-Schiff (PAS) staining in ( A ) the upper vaginal tract and ( B ) the lower vaginal tract, respectively, at 0.5 dpc in Esr1 f/f and Wnt7a cre/+ ; Esr1 f/f animals. Black arrows indicate a site of cell infiltration from stromal and epithelial layers into the vaginal lumen. Yellow arrowheads indicate the distinct cornified layer in Esr1 f/f tissues. Black arrowheads denote the PAS-positive cell on the apical side of Wnt7a cre/+ ; Esr1 f/f vaginal lumen. Insets are negative control without primary antibodies. Scale bars = 100 um. n = 4-6 mice/genotype.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig 7 Endogenous gene expression of KLK and serine protease inhibitor ( SPINK ) in human cell lines. A. Expression levels of KLK1-15 , SPINK5 and SPINK6 in immortalized human ectocervical (hEct1), endocervical (hEnd1), uterine adenocarcinoma (Ishikawa), and prostate cancer (LNCaP) cells in the absence of any hormonal treatment. Note that undetectable values were plotted as expression levels of 0 in the graph. B. Transcript levels of ESR1 normalized to RPL5 in hEct1, hEnd1, Ishikawa, and LNCaP cell lines. ** p

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2. Representative images of immunohistochemical staining for (A) STS, (B) SULT1E1 and (C) ERalpha subsequent in paraffin-embedded sections from serous epithelial ovarian cancer. Cytoplasmic staining was observed for STS and SULT1E1, while ERalpha staining was predominantly observed in the nucleus. Respective images from negative control samples for (D) STS, (E) SULT1E1 and (F) ERalpha. Magnification, x200. STS, steroid sulfatase; SULT1E1, estrogen sulfotransferase; ERalpha, estrogen receptor alpha.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig 1 Loss of ESR1 in uterine epithelial cells leads to a semen liquefaction defect in female mice. A. Immunohistochemical analysis indicates the expression of ESR1 in the uteri of Esr1 f/f mice and its absence in Wnt7a Cre/+ ; Esr1 f/f animals at 0.5 dpc. LE = luminal epithelial cells; GE = glandular epithelial cells. B. Gross morphology of the reproductive tract of Esr1 f/f and Wnt7a Cre/+ ; Esr1 f/f animals at 0.5 dpc. C. Diameters of the uteri prior to dissection at 0.5 dpc, n = 9-10 mice/genotype. D. Total fluid volume (muL) collected from the uteri of Esr1 f/f and Wnt7a Cre/+ ; Esr1 f/f animals at 0.5 dpc, n = 3 mice/genotype. E. Expression of Aqp transcripts in the uteri at 0.5 dpc, n = 4-5 mice/genotype. F. Liquefaction time measured as the time it took for semen collected from the uteri at 0.5 dpc to fill a 25-muL capillary tube (minutes). Note that the liquefaction time for semen collected from Wnt7a Cre/+ ; Esr1 f/f uteri was over 1 h (>60 min), n = 3 mice/genotype. G. Hematoxylin & Eosin (H&E) staining of uterine samples collected at 0.5 dpc, n = 9-10 mice/genotype. H. Sperm density was determined from the semen within the uterine lumen per microscopic field of H&E stained images and normalized to mm 2 , n = 7-10 mice/genotype. I. Total sperm number in the uterus was calculated after normalization to the total fluid volume collected, n = 3 mice/genotype. J. Percentage of sperm motility per microscopic field in the semen collected from the uteri of Esr1 f/f and Wnt

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig 2 Gel-forming proteins are not degraded in Wnt7a Cre/+ ; Esr1 f/f uteri. A. Masson's Trichrome staining of uterine cross-sections of Esr1 f/f and Wnt7a Cre/+ ; Esr1 f/f collected at 0.5 dpc. Blue staining represents collagen or connective tissues; red staining represents cytoplasm and muscle fibers. Representative images are shown. B. Immunoblotting of semenogelin 1 (SEMG1) in the uteri of Esr1 f/f and Wnt7a Cre/+ ; Esr1 f/f at 0.5 dpc. Upper bands indicate size of full length SEMG1 (~52 kDa). Lower bands indicate the product size of cleaved SEMG1. beta-Actin was used as a loading control (n = 3-4 mice/genotype). C. Quantitative analysis of SEMG1 protein expression normalized to beta-Actin. *** p

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig 4 Luminal epithelial, glandular epithelial, and stromal cell proliferation in Esr1 f/f and Wnt7a Cre/+ ; Esr1 f/f animals at 0.5 dpc. A. Immunohistochemical staining (IHC) of Ki67 (proliferative marker) in the uterine cross-section from Esr1 f/f and Wnt7a Cre/+ ; Esr1 f/f animals. Representative images are shown. B. Percentage of Ki67 positive cells of total cell type counted within microscopic field (3 fields per animal). Total cell counts for luminal epithelium (LE), glandular epithelium (GE), and stroma are 3058, 939, and 9160 cells, respectively. C. Expression of Foxa2 in whole uteri collected from Esr1 f/f and Wnt7a Cre/+ ; Esr1 f/f animals at 0.5 dpc determined by real-time RT-PCR analysis. * p