NB200-347

antibody from Novus Biologicals

Targeting: ESR1 ER-alpha, Era, ESR, NR3A1

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

NB200-347

Provider

Novus Biologicals

Proper citation

Novus Cat#NB200-347, RRID:AB_10000682

Product name

Rabbit Polyclonal ER alpha/NR3A1 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 ul

Concentration

1.0 mg/ml

Storage

Store at 4C. Do not freeze.

References

Submitted references

Lactoferrin-endothelin-1 axis contributes to the development and invasiveness of triple-negative breast cancer phenotypes.

Ha NH, Nair VS, Reddy DN, Mudvari P, Ohshiro K, Ghanta KS, Pakala SB, Li DQ, Costa L, Lipton A, Badwe RA, Fuqua S, Wallon M, Prendergast GC, Kumar R
Cancer research 2011 Dec 1;71(23):7259-69

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Identification of a novel estrogen receptor-alpha variant and its upstream splicing regulator.

Ohshiro K, Mudvari P, Meng QC, Rayala SK, Sahin AA, Fuqua SA, Kumar R
Molecular endocrinology (Baltimore, Md.) 2010 May;24(5):914-22

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MicroRNA cluster 221-222 and estrogen receptor alpha interactions in breast cancer.

Di Leva G, Gasparini P, Piovan C, Ngankeu A, Garofalo M, Taccioli C, Iorio MV, Li M, Volinia S, Alder H, Nakamura T, Nuovo G, Liu Y, Nephew KP, Croce CM
Journal of the National Cancer Institute 2010 May 19;102(10):706-21

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The cell fate determination factor DACH1 is expressed in estrogen receptor-alpha-positive breast cancer and represses estrogen receptor-alpha signaling.

Popov VM, Zhou J, Shirley LA, Quong J, Yeow WS, Wright JA, Wu K, Rui H, Vadlamudi RK, Jiang J, Kumar R, Wang C, Pestell RG
Cancer research 2009 Jul 15;69(14):5752-60

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Cathepsin L inhibition suppresses drug resistance in vitro and in vivo: a putative mechanism.

Zheng X, Chu F, Chou PM, Gallati C, Dier U, Mirkin BL, Mousa SA, Rebbaa A
American journal of physiology. Cell physiology 2009 Jan;296(1):C65-74

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ER alpha/NR3A1 Antibody [NB200-347] - Samples: MCF7 WCE (5, 15, 50 ug for WB; 1 mg for IP, 1/4 of IP loaded). Antibodies: NB 200-344, NB 200-345, NB 200-346, and NB 200-347, each used at 3 ug/ml lysate for IP. ER alpha was blotted (WB and IP) using NB 200-347 (0.1 ug/ml). ECL: 3 minutes.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ER alpha/NR3A1 Antibody [NB200-347] - Detection of Human Estrogen Receptor Alpha by Western Blot. Samples: Whole cell lysate (50 ug) from MCF7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Estrogen Receptor Alpha antibody NB200-347 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation: ER alpha/NR3A1 Antibody [NB200-347] - Detection of human ER alpha/NR3A1 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-ER alpha/NR3A1 antibody NB200-347 (lot 3) used for IP at 6 ug per reaction. ER alpha/NR3A1 was also immunoprecipitated by previous lot of this antibody (lot 2). For blotting immunoprecipitated ER alpha/NR3A1, NB200-347 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.