Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-16476 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Estrogen Receptor alpha Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA5-16476 targets Estrogen Receptor in IF, IHC (P), IP, and WB applications and shows reactivity with mouse, Rabbit, Rat, Bovine, Human, Ovine, and Porcine samples.
- Concentration
- 0.25 mg/mL
Submitted references A High-Cholesterol Diet Increases 27-Hydroxycholesterol and Modifies Estrogen Receptor Expression and Neurodegeneration in Rabbit Hippocampus.
Anti-inflammatory effects of a Chinese herbal medicine in atherosclerosis via estrogen receptor β mediating nitric oxide production and NF-κB suppression in endothelial cells.
Testosterone administration to adult rats differentially modulates androgen and oestrogen receptor-α expression in reproductive organs and pituitary.
The scaffold protein MEK Partner 1 is required for the survival of estrogen receptor positive breast cancer cells.
Brooks SW, Dykes AC, Schreurs BG
Journal of Alzheimer's disease : JAD 2017;56(1):185-196
Journal of Alzheimer's disease : JAD 2017;56(1):185-196
Anti-inflammatory effects of a Chinese herbal medicine in atherosclerosis via estrogen receptor β mediating nitric oxide production and NF-κB suppression in endothelial cells.
Wang L, Qiu XM, Hao Q, Li DJ
Cell death & disease 2013 Mar 21;4(3):e551
Cell death & disease 2013 Mar 21;4(3):e551
Testosterone administration to adult rats differentially modulates androgen and oestrogen receptor-α expression in reproductive organs and pituitary.
Kaushik MC, Misro MM, Sehgal N, Nandan D
Andrologia 2012 May;44 Suppl 1:312-22
Andrologia 2012 May;44 Suppl 1:312-22
The scaffold protein MEK Partner 1 is required for the survival of estrogen receptor positive breast cancer cells.
Marina M, Wang L, Conrad SE
Cell communication and signaling : CCS 2012 Jul 9;10(1):18
Cell communication and signaling : CCS 2012 Jul 9;10(1):18
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Estrogen Receptor (ER) was performed by loading the indicated amounts of recombinant full-length human ER alpha (Product # A15674) and ER beta (Product # A15664) proteins, and 10 µL of PageRuler Prestained Protein Ladder (Product # 26616) per well onto a Novex® 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a PVDF membrane (Product # 88518) using the G2 Blotter (Product # 62288), and blocked with 5% BSA in TBST for at least 1 hour at room temp. ER alpha was detected at ~66 kD (left panel) using an ER polyclonal antibody (Product # PA5-16476) at a dilution of 1:1000 in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-rabbit IgG secondary antibody (Product # 31460) at a dilution of 1:40,000. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080). Directly alongside the above blot, a second blot containing the same recombinant ER alpha and ER beta protein samples was probed with an ER-beta specific polyclonal antibody (Product # PA1-313, right panel) at a dilution of 1:2000 overnight at 4C, followed by an HRP-conjugated goat anti-rabbit IgG secondary antibody (Product # 31460) at a dilution of 1:40,000. Chemiluminescent detection was also performed using SuperSignal West Pico (Product # 34080).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of Estrogen Receptor using Estrogen Receptor Polyclonal Antibody (Product # PA5-16476) on MCF-7 Cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Estrogen Receptor (ER, green) in T47D and Hs 578T breast cancer cells. The cells were fixed with 4% paraformaldehyde for 15 min at room temp, permeabilized with 0.1% Triton X-100 for 15 min at room temp, and blocked with 0.3% BSA in PBS for at least 30 min at room temp. Cells were stained with an ER polyclonal antibody (Product # PA5-16476) at a dilution of 1:100 in blocking buffer overnight at 4C, and then incubated with a DyLight 488-conjugated goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:500 for at least 1 hour at room temp. Nuclei (blue) were stained with DAPI (Product # 62247). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human breast carcinoma stained with ER, alpha antibody7 using peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of Estrogen Receptor using Estrogen Receptor Polyclonal Antibody (Product # PA5-16476) on denatured Human MCF-7 Cells.