PA5-16476

antibody from Invitrogen Antibodies

Targeting: ESR1 ER-alpha, Era, ESR, NR3A1

Antibody data

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Validation data

Product number: PA5-16476 - Provider product page
Provider: Invitrogen Antibodies
Product name: Estrogen Receptor alpha Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: PA5-16476 targets Estrogen Receptor in IF, IHC (P), IP, and WB applications and shows reactivity with mouse, Rabbit, Rat, Bovine, Human, Ovine, and Porcine samples.
Concentration: 0.25 mg/mL

ESR1 protein structure - PA5-16476 shown in red.

Brooks SW, Dykes AC, Schreurs BG
Journal of Alzheimer's disease : JAD 2017;56(1):185-196

Wang L, Qiu XM, Hao Q, Li DJ
Cell death & disease 2013 Mar 21;4(3):e551

Kaushik MC, Misro MM, Sehgal N, Nandan D
Andrologia 2012 May;44 Suppl 1:312-22

Marina M, Wang L, Conrad SE
Cell communication and signaling : CCS 2012 Jul 9;10(1):18

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Estrogen Receptor (ER) was performed by loading the indicated amounts of recombinant full-length human ER alpha (Product # A15674) and ER beta (Product # A15664) proteins, and 10 µL of PageRuler Prestained Protein Ladder (Product # 26616) per well onto a Novex® 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a PVDF membrane (Product # 88518) using the G2 Blotter (Product # 62288), and blocked with 5% BSA in TBST for at least 1 hour at room temp. ER alpha was detected at ~66 kD (left panel) using an ER polyclonal antibody (Product # PA5-16476) at a dilution of 1:1000 in blocking buffer overnight at 4C on a rocking platform, followed by an HRP-conjugated goat anti-rabbit IgG secondary antibody (Product # 31460) at a dilution of 1:40,000. Chemiluminescent detection was performed using SuperSignal West Pico (Product # 34080). Directly alongside the above blot, a second blot containing the same recombinant ER alpha and ER beta protein samples was probed with an ER-beta specific polyclonal antibody (Product # PA1-313, right panel) at a dilution of 1:2000 overnight at 4C, followed by an HRP-conjugated goat anti-rabbit IgG secondary antibody (Product # 31460) at a dilution of 1:40,000. Chemiluminescent detection was also performed using SuperSignal West Pico (Product # 34080).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot of Estrogen Receptor using Estrogen Receptor Polyclonal Antibody (Product # PA5-16476) on MCF-7 Cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunofluorescent analysis of Estrogen Receptor (ER, green) in T47D and Hs 578T breast cancer cells. The cells were fixed with 4% paraformaldehyde for 15 min at room temp, permeabilized with 0.1% Triton X-100 for 15 min at room temp, and blocked with 0.3% BSA in PBS for at least 30 min at room temp. Cells were stained with an ER polyclonal antibody (Product # PA5-16476) at a dilution of 1:100 in blocking buffer overnight at 4C, and then incubated with a DyLight 488-conjugated goat anti-rabbit IgG secondary antibody (Product # 35552) at a dilution of 1:500 for at least 1 hour at room temp. Nuclei (blue) were stained with DAPI (Product # 62247). Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Formalin-fixed, paraffin-embedded human breast carcinoma stained with ER, alpha antibody7 using peroxidase-conjugate and AEC chromogen. Note nuclear staining of tumor cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunoprecipitation of Estrogen Receptor using Estrogen Receptor Polyclonal Antibody (Product # PA5-16476) on denatured Human MCF-7 Cells.