MA5-13032

antibody from Invitrogen Antibodies

Targeting: ERBB2 CD340, HER-2, HER2, NEU, NGL

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-13032

Provider

Invitrogen Antibodies

Product name

ErbB2 (HER-2) Monoclonal Antibody (N28)

Antibody type

Monoclonal

Antigen

Other

Description

MA5-13032 targets HER-2 in IP applications and shows reactivity with Human samples.

Antibody clone number

N28

Concentration

0.2 mg/mL

References

Submitted references

Clathrin-independent endocytosis of ErbB2 in geldanamycin-treated human breast cancer cells.

Barr DJ, Ostermeyer-Fay AG, Matundan RA, Brown DA
Journal of cell science 2008 Oct 1;121(Pt 19):3155-66

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of Her2 was performed using 70% confluent log phase SK-BR-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Her2 Mouse monoclonal Antibody (Product # MA5-13032) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree Celsius and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membranous and cytoplasmic localization. Panel f represents MDAMB-231 cells as negative controls, showing no Her2 staining. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.